Abstract:
:Disruption of cell-matrix interactions can lead to anoikis-apoptosis due to loss of matrix contacts. We previously showed that Nerve/glial antigen 2 (NG2) is a novel anoikis receptor. Specifically, overexpression of NG2 leads to anoikis propagation, whereas its suppression leads to anoikis attenuation. Interestingly, NG2 expression decreases in late anoikis, suggesting that NG2 reduction is also critical to this process. Thus, we hypothesized that NG2 undergoes cleavage to curtail anoikis propagation. Further, since matrix metalloproteinases (MMPs) cleave cell surface receptors, play a major role in modulating apoptosis, and are associated with death receptor cleavage during apoptosis, we further hypothesized that cleavage of NG2 could be mediated by MMPs to regulate anoikis. Indeed, anoikis conditions triggered release of the NG2 extracellular domain into condition media during late apoptosis, and this coincided with increased MMP-13 expression. Treatment with an MMP-13 inhibitor and MMP-13 siRNA increased anoikis, since these treatments blocked NG2 release. Further, NG2-positive cells exhibited increased anoikis upon MMP-13 inhibition, whereas MMP-13 inhibition did not increase anoikis in NG2-null cells, corroborating that retention of NG2 on the cell membrane is critical for sustaining anoikis, and its cleavage for mediating anoikis attenuation. Similarly, NG2 suppression with siRNA inhibited NG2 release and anoikis. In contrast, MMP-13 overexpression or exogenous MMP-13 reduced anoikis by more effectively shedding NG2. In conclusion, maintenance of NG2 on the cell surface promotes anoikis propagation, whereas its shedding by MMP-13 actions attenuates anoikis. Given that these findings are derived in the context of periodontal ligament fibroblasts, these data have implications for periodontal inflammation and periodontal disease pathogenesis.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Joo NE,Miao D,Bermúdez M,Stallcup WB,Kapila YLdoi
10.1089/dna.2014.2399subject
Has Abstractpub_date
2014-12-01 00:00:00pages
854-62issue
12eissn
1044-5498issn
1557-7430journal_volume
33pub_type
杂志文章abstract::Liver-expressed chemokine (LEC) is a CC chemokine that is selectively expressed in the liver. We report here the structures of the human and mouse genes for LEC. The human LEC gene (SCYA16) was isolated from a bacterial artificial chromosome (BAC) clone that also contained CC chemokine genes for MPIF-1/Ckbeta8, HCC-2/...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315330
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abstract::In this study, we isolated two cDNA molecules encoding putative glutamate receptor subunits, fGluR1 alpha and fGluR1 beta, from an Oreochromis sp. brain cDNA library by hybridizing with the glutamate receptor cDNA, fGluR2 beta, of the same fish. The deduced amino acid sequence of the fGluR1 alpha consists of 908 resid...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.717
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pub_type: 杂志文章
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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doi:10.1089/104454904322745907
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journal_title:DNA and cell biology
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doi:10.1089/104454903321908629
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abstract::The breast cancer gene 1 (BRCA1) is a tumor suppressor, and mutations or epigenetic inactivation will increase the risk of breast cancer oncogenesis. The current research aimed to explore the relationship between BRCA1 expression, prognosis, and tumor immunity in hepatocellular carcinoma (HCC). In this study, BRCA1 ex...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5644
更新日期:2020-10-01 00:00:00
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journal_title:DNA and cell biology
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更新日期:2005-03-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.609
更新日期:1995-07-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5066
更新日期:2020-08-01 00:00:00
abstract::The epidermal growth factor (EGF) pathway stimulates proliferation and differentiation of epidermal and epithelial tissues, and plays an important role in tumorigenesis. The association between EGF polymorphisms and cancer risk is controversial; thus, we performed this meta-analysis. Overall, 41 case-control studies w...
journal_title:DNA and cell biology
pub_type: 杂志文章,meta分析
doi:10.1089/dna.2011.1394
更新日期:2012-04-01 00:00:00
abstract::Quantitative determination of T-cell receptor (TCR) V beta expression is necessary to define the changes in TCR-V beta subfamily expression that occur during T-cell maturation and selection and to detect alterations of the TCR-V beta repertoire that may be associated with human diseases. Here we describe and validate ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.217
更新日期:1993-04-01 00:00:00
abstract::Glucose-stimulated insulin gene transcription is mainly regulated by a 340-bp promoter region upstream of the transcription start site by beta-cell-enriched transcription factors Pdx-1, MafA, and NeuroD1. Previous studies have shown that histone H4 hyperacetylation is important for acute up-regulation of insulin gene ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1336
更新日期:2012-01-01 00:00:00
abstract::We have analyzed transcription factor-mediated DNA supercoiling catalyzed by the Xenopus oocyte extract (S-150). Under conditions that inhibit endogenous supercoiling activity (2 mM EDTA), the 5S RNA specific transcription factor, TFIIIA, promotes a negative change in DNA linking number. The SV40 binding protein, T an...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1991.10.223
更新日期:1991-04-01 00:00:00
abstract::Electroporation has proven to be a highly effective technique for the in vivo delivery of genes to a number of solid tissues. In most of the reported methods, DNA is injected into the target tissue and electrodes are placed directly on or in the tissue for application of the electric field. While this works well for s...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454903322625000
更新日期:2003-12-01 00:00:00
abstract::We identified extracellular signal-regulated kinase 2 (erk2) from green mud crab, Scylla paramamosain, in this article. It was originally identified from an expressed sequence tag fragment from a normalized gonadal cDNA library. 5' Rapid amplification of cDNA end (RACE) technique was used to obtain the 5' untranslated...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1458
更新日期:2012-07-01 00:00:00
abstract::Epulopiscium sp. type B, a member of the Firmicutes, is a large (up to 300 microm), cigar-shaped bacterial symbiont of surgeonfish that propagates itself by forming multiple intracellular offspring. This unusual form of reproduction is an apparent modification of a developmental program used by some Firmicutes to prod...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2008.0801
更新日期:2009-02-01 00:00:00
abstract::Osteosarcoma is one of the most common primary malignant tumors of the bone and tends to develop in teenage years. Although multitreatments for the diagnosis and therapy of osteosarcoma have been developed, there are still needs of new methods to prevent and treat the osteosarcoma. Here, we performed bioinformatic ana...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5377
更新日期:2020-07-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.729
更新日期:1993-10-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454903322405464
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abstract::The impact of chromatin topology on the DNA synthetic process was studied in the human squamous-cell carcinoma cell line SQ-20B. A 1-h exposure < or = 10 microM VP16 produced an increase in DNA supercoil tension, measured by recording laser light scatter from salt-extracted nuclei. This change was precisely paralleled...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.613
更新日期:1998-07-01 00:00:00
abstract::We have isolated and sequenced cDNA clones that encode human spermine synthase (EC 2.5.1.22). The total length of the sequenced cDNA was 1,612 nucleotides, containing an open reading frame encoding a polypeptide chain of 368 amino acids. All of the previously sequenced peptide fragments of human and bovine spermine sy...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.841
更新日期:1995-10-01 00:00:00
abstract::The activation of H-plk (Human-proviral linked Krüppel), a human Krüppel-related zinc finger gene in organs such as placenta, adrenal cortex, and testis, is probably due to insertion of an endogenous retrovirus, ERV3, upstream of the gene. Several differently spliced transcripts originate from this locus, e.g., a tran...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.27
更新日期:1998-01-01 00:00:00
abstract::Screening of a mouse kidney cDNA library with a HNF-3/fork head domain probe revealed cDNA Hfh-1L containing the highly conserved fork head DNA-binding domain. The Hfh1L cDNA shows 92.7% homology at the nucleic acid level with the fork head gene HFH-1 from rat. Southern blot analyses demonstrated that the Hfh-1L gene ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.679
更新日期:1998-08-01 00:00:00
abstract::DNA methylation of CYP17 (steroid 17 alpha-hydroxylase) was studied in bovine adrenocortical cells, which lose the capacity to express this tissue-specific gene in culture by phenotypic switching. Restriction enzyme digestions, and sequencing of a lambda clone of a second CYP17 gene (CYP17A2), showed that there are at...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.385
更新日期:1992-06-01 00:00:00
abstract::The aim of this article is to review our current understanding of the role of cytokines, chemokines, T cells, Langerhans cells, and neutrophils (PMN) and their interactions in vivo in the host response to Pseudomonas aeruginosa ocular challenge. The cellular/cytokine network in vivo has begun to be unraveled, and the ...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
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abstract::Alzheimer's disease (AD) is a neurodegenerative disorder characterized by overproduction of beta-amyloid (Abeta), which is formed from amyloid precursor protein (APP), with the subsequent pathologic deposition of Abeta in regions of the brain important for memory and cognition. Recently, vaccination of murine models o...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490152717587
更新日期:2001-11-01 00:00:00
abstract::Clusterin (CLU) and clathrin assembly lymphoid myeloid (CALM) protein are implicated in the function of neuronal synapses. However, to our knowledge, whether they play roles in the maldevelopment of synaptic pathways in schizophrenia has not been studied. The purpose of this study was to examine whether single nucleot...
journal_title:DNA and cell biology
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doi:10.1089/dna.2010.1075
更新日期:2010-12-01 00:00:00
abstract::It is widely believed that cellular senescence is a tumor suppressor mechanism; however, it has not been understood why it is advantageous for organisms to retain mutant cells is a postmitotic state rather than simply eliminating them by apoptosis. It has recently been proposed that the primary role of cellular senesc...
journal_title:DNA and cell biology
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doi:10.1089/dna.2006.25.69
更新日期:2006-02-01 00:00:00