Abstract:
:Insulin-like growth factor I (IGF-I) plays a major role in development and metabolism. Currently, the cDNA-derived primary structure of IGF-I is known for some mammals and for chicken, frog, and salmon. Additionally, the organization of the human, rat, and chicken IGF-I genes has been established. The investigation of IGF-I gene structure in fish would extend the evolutionary picture for this hormone and facilitate our understanding of the features of the IGF-I gene that are common to all vertebrate species. The cloned chum salmon IGF-I gene appears to be much more compact than the mammalian and avian genes, being less than 20 kb in length. As in other species, however, the mature IGF-I peptide appears to consist of 70 amino acids and is encoded by exons 2 and 3. Intriguingly, exon 1-encoded 5'-untranslated region sequences are highly conserved, while the coding sequences at the 3' end of the same exon are less conserved. The amino terminus of the signal peptide is four amino acids shorter than in the mammalian and avian peptides. The end of the B domain, the C, A, and D domains, and the first part of the E peptide are encoded by exon 3, but the exon 3-encoded E peptide sequence is 27 amino acids longer than in other species. These extra 27 amino acids, encoded by both coho and chum salmon cDNAs, may be deleted by alternative splicing, as suggested from the sequence of a coho salmon IGF-I cDNA.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Kavsan VM,Koval AP,Grebenjuk VA,Chan SJ,Steiner DF,Roberts CT Jr,LeRoith Ddoi
10.1089/dna.1993.12.729subject
Has Abstractpub_date
1993-10-01 00:00:00pages
729-37issue
8eissn
1044-5498issn
1557-7430journal_volume
12pub_type
杂志文章abstract::We have cloned and mapped the chromosomal location of three novel human genes encoding G protein-coupled receptors that we have named GPR6, GPR5, and GPR4. The entire coding region for each of these genes was contained on single exons. Gene GPR6 encoded a receptor that shared closest identity (71% in the transmembrane...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.25
更新日期:1995-01-01 00:00:00
abstract::The CDC6 gene product from Saccharomyces cerevisiae is required for transition from late G1 to S phase of the cell cycle. We have investigated the subcellular localization of the CDC6 protein in yeast to explore where Cdc6p exerts its gene function (s). Using affinity-purified sera we localized Cdc6p to the cytoplasm ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.883
更新日期:1996-10-01 00:00:00
abstract::We previously showed that the human AKNA gene encodes an AT-hook transcription factor that regulates the expression of costimulatory cell surface molecules on lymphocytes. However, AKNA cDNA probes hybridize with multiple transcripts, suggesting either the existence of other homologous genes or a complex regulation op...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.325
更新日期:2005-05-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.985
更新日期:1997-08-01 00:00:00
abstract::Parkinson's disease (PD) is a serious neurodegenerative disorder that lacks effective therapeutic methods. In this research, expressions of PPARα, RXRα, and miR-21 were evaluated in PD patients and normal controls. To investigate the effects of miR-21, docosahexaenoic acid (DHA) and aspirin (ASA) on PD, as well as the...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3643
更新日期:2017-06-01 00:00:00
abstract::Gene expression is modulated by epigenetic factors that come in varying forms, such as DNA methylation, histone modifications, microRNAs, and long noncoding RNAs. Recent studies reveal that these epigenetic marks are important regulatory factors in brain function. In particular, DNA methylation dynamics are found to b...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2011.1565
更新日期:2012-10-01 00:00:00
abstract::We have cloned and sequenced the cDNA corresponding to the rat preproepidermal growth factor (ppEGF) mRNA. The cDNA contained 4,801 nucleotides, similar to that reported for the mouse (4,749 nucleotides) and the human mRNAs (4,871 nucleotides). The predicted protein sequence would contain 1,133 amino acids, smaller th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.481
更新日期:1992-07-01 00:00:00
abstract::Porifera (sponges) constitute the lowest metazoan phylum. Experiments examined whether sponges can recognize self/nonself molecules. Cells from the marine sponge Suberites domuncula were incubated with membranes from either S. domuncula or another marine sponge, Geodia cydonium, as well as with recombinant alpha-integ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999314746
更新日期:1999-12-01 00:00:00
abstract::The promoters of mammalian striated muscle actin gene contain binding sites for a number of transcription factors. Examples are the CArG boxes, which bind a protein identical to or related to serum response factor (SRF), E boxes, which bind myogenic determination factors such as MyoD and myogenin, and -CCGCCC- motifs,...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.609
更新日期:1995-07-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.381
更新日期:2005-06-01 00:00:00
abstract::The gene for the bovine oxytocin receptor has been sequenced using a combination of clones derived from a bovine endometrial cDNA library from estrus and a bovine genomic DNA library, with confirmation of structure using reverse transcription PCR programmed by term myometrial RNA. The receptor belongs to the seven tra...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.1037
更新日期:1995-12-01 00:00:00
abstract::The long arm of chromosome 6 is frequently deleted in diverse human neoplasms. Our previous study showed a minimum deletion region between markers D6S1056 and D6S300 on chromosome 6q in primary prostate cancer (CaP). In this study, we further refined a 200-kb minimal region of deletion (6qTSG1) centered around D6S1013...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.25.597
更新日期:2006-11-01 00:00:00
abstract::Sepsis is an inflammatory-related disease, and severe sepsis would induce multiorgan dysfunction, which is the most common cause of death of patients in noncoronary intensive care units. Progression of novel therapeutic strategies has proven to be of little impact on the mortality of severe sepsis, and unfortunately, ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3944
更新日期:2018-02-01 00:00:00
abstract::Interstitial lung disease (ILD) is the main reason of death in patients with systemic sclerosis (SSc). The potential microRNA (miRNA)-messenger RNA (mRNA) interaction networks of SSc-ILD from a systematic biological perspective are unclear. To characterize differentially expressed miRNAs (DE-miRNAs) and differentially...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4780
更新日期:2019-09-01 00:00:00
abstract::Heme plays a significant pathogenic role in several diseases involving the kidney. The cellular content of heme, derived either from the delivery of filtered heme proteins such as hemoglobin and myoglobin, or from the breakdown of ubiquitous intracellular heme proteins, is regulated via the heme oxygenase enzyme syste...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454902753759726
更新日期:2002-04-01 00:00:00
abstract::Myocardial ischemic disease affects the prognosis in perioperative patients. Diabetes can aggravate myocardial injury. The purpose of this research is to investigate the effect of ferroptosis in the process of diabetes mellitus (DM) myocardial ischemia/reperfusion (I/R) injury (IRI). Endoplasmic reticulum stress (ERS)...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5097
更新日期:2020-02-01 00:00:00
abstract::We examined the chromosomal basis for the synthesis of tissue (ovary, endometrium/placenta, and peri-implantation blastocyst) isoforms of cytochrome P450 aromatase in the pig. DNA fragments derived from three distinct porcine aromatase chromosomal genes were cloned and characterized. The porcine type III aromatase gen...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.769
更新日期:1997-06-01 00:00:00
abstract::Hepatocyte nuclear factor-1alpha (HNF-1alpha) is a homeodomain-containing transcription factor regulating the expression of liver and pancreas-specific genes. Mutations in the HNF-1alpha-encoding gene TCF1 cause maturity-onset diabetes of the young, type 3 (MODY3). These mutations may affect nuclear import or reduce t...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.661
更新日期:2005-11-01 00:00:00
abstract::This research aims to investigate the immune-associated gene signature from databases to improve the prognostic value in hepatocellular carcinoma (HCC) by multidimensional methods using various bioinformatic methods. Fifty-one immune-associated genes were mined out, which were associated with clinical characters throu...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2019.5099
更新日期:2020-04-01 00:00:00
abstract::Long noncoding RNA host gene 16 (SNHG16) has a key role in a variety of cancer progression. However, the role and mechanism of SNHG16 in osteosarcoma (OS) remain unknown. In this study, we examined the functional role of SNHG16 in OS cells through knocked-down SNHG16 by using siRNA. We found that SNHG16 is overexpress...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4424
更新日期:2019-02-01 00:00:00
abstract::Sequence analysis of the 5' end of the thrombomodulin (TM) gene has identified a difference in two strains of laboratory mice, BALB/cBD (maintained at the Oak Ridge National Laboratory) and the strain used for the sequence listed in the GenBank data base. An intracisternal A-particle (IAP) provirus was present in the ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.311
更新日期:1993-05-01 00:00:00
abstract::dTMP kinase (E.C.2.7.4.9.) catalyzes the phosphorylation of dTMP to the corresponding diphosphate. This enzyme is essential for DNA synthesis in vivo and is an important intermediate enzyme in the pathway of many pyrimidine analog drugs. In this report, we describe the isolation of the human dTMP kinase gene by functi...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.461
更新日期:1994-05-01 00:00:00
abstract:PURPOSE:This case-control study was conducted in order to evaluate the potential role of polymorphic genes encoding enzymes involved in estrogen biosynthesis (CYP19A1) and metabolism (GSTM1, GSTT1, and GSTP1), and their action in modulating individual susceptibility to breast cancer. METHODS:Genomic DNA was extracted ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1538
更新日期:2012-06-01 00:00:00
abstract::The G0S19 genes are members of the "small inducible" family of genes, which have similar exon-intron organizations and encode secreted proteins with similar dispositions of cysteine and proline residues. G0S19-1 mRNA is increased shortly after the addition of lectin or cycloheximide to cultured human blood mononuclear...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.589
更新日期:1990-10-01 00:00:00
abstract::To explore the effect of miR-106a in breast cancer cell behavior and sensitivity to chemotherapeutic agents. Tumor tissue and adjacent normal tissue were derived from 40 breast cancer patients, and miR-106a expression was measured by reverse transcription-qPCR. Breast cancer cells, MDA-MB-231 and MCF-7, were treated w...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4282
更新日期:2019-02-01 00:00:00
abstract::The aim of this work was to express a eukaryotic pre-protein in Escherichia coli so that it could be obtained intact, without cleavage, by bacterial leader peptidase. To this end, cDNA coding for honeybee prepromelittin was ligated to the 3' end of genes coding for truncated forms of either Protein A or beta-galactosi...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.875
更新日期:1994-08-01 00:00:00
abstract::We have screened an Aplysia atrial gland cDNA library using a prohormone convertase (PC)1 probe prepared by polymerase chain reaction (PCR) and have isolated an Aplysia PC1-related full-length 3.6-kb cDNA clone. The cDNA sequence (3,565 bp) encoded a putative preproendoprotease (APC1) of 703 amino acid residues that s...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.339
更新日期:1996-04-01 00:00:00
abstract::The recent explosion of scientific and technical knowledge in the field of molecular biology has allowed us to make important advances in our understanding of the molecular basis of many human diseases. This technology has now entered the clinical laboratory where identification of specific genetic sequences can aid i...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.1993.12.455
更新日期:1993-06-01 00:00:00
abstract::The liver has an excellent capacity for regeneration when faced with external injury and the damage differs from that of other organs in the body. Our aim was to identify the role of circular RNA (circRNA) during the DNA synthesis phase (36 h) of mice liver regeneration. High-throughput RNA sequencing was conducted to...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5750
更新日期:2020-11-01 00:00:00
abstract::Polymorphisms such as single-nucleotide polymorphisms (SNPs) and insertions/deletions (Indels) can be associated with phenotypic traits and be used as markers for disease diagnosis. Identification of these genetic variations within laboratory mice is crucial to improve our understanding of the genetic background of th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1353
更新日期:2012-03-01 00:00:00