Abstract:
:We have screened an Aplysia atrial gland cDNA library using a prohormone convertase (PC)1 probe prepared by polymerase chain reaction (PCR) and have isolated an Aplysia PC1-related full-length 3.6-kb cDNA clone. The cDNA sequence (3,565 bp) encoded a putative preproendoprotease (APC1) of 703 amino acid residues that showed considerable sequence identity with other eukaryotic PC1s, and indicated a high degree of sequence identity with an Aplysia nervous system PC sequence (aPC1B). Northern blot analysis of atrial gland RNA identified two APC1 transcripts of 3.9 kb and 5.0 kb. APC1 is a candidate PC that may play an important role in the processing of egg-laying hormone (ELH)-related precursors in atrial gland secretory cells and represents one of the first examples of PC1 expression in an exocrine tissue.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Gorham EL,Nagle GT,Smith JS,Shen H,Kurosky Adoi
10.1089/dna.1996.15.339subject
Has Abstractpub_date
1996-04-01 00:00:00pages
339-45issue
4eissn
1044-5498issn
1557-7430journal_volume
15pub_type
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