Abstract:
:Phytoplasmas are nonculturable cell wall-less, obligate intracellular pathogens of plants and insect vectors. In their descent from walled bacterial ancestors, phytoplasmas underwent massive genome reduction, resulting in some of the smallest cellular genomes known in nonsymbiotic bacteria. While requirements for in vitro culture of phytoplasmas remain unknown, two opposing reports have appeared concerning genes encoding the ability of phytoplasmas to synthesize folates de novo. One study found pseudogene homologs of folP and folK, obviating folate synthesis in "Candidatus Phytoplasma asteris"-related strain CPh, whereas, a separate study found intact genes encoding a complete folate biosynthesis pathway in "Ca. Phytoplasma asteris"-related strain OY. To resolve the apparent conflict, we hypothesized that evolutionary adaptation to the availability of folate and/or other metabolites in host cells is an ongoing process in the phytoplasma clade that is reflected in part by differences among phytoplasmas in the status of genes of the folate biosynthesis pathway. By studying folP and folK loci in 11 closely related phytoplasmas, we determined that these essential folate biosynthesis genes are intact in some phytoplasmas but are deteriorating in closely related strains. We suggest that the status of the folate biosynthesis pathway and the course of gene decay are lineage-specific, predicting the eventual, lineage-related loss of recognizable folP and folK homologs in phytoplasma genomes.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Davis RE,Jomantiene R,Zhao Ydoi
10.1089/dna.2005.24.832keywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
832-40issue
12eissn
1044-5498issn
1557-7430journal_volume
24pub_type
杂志文章abstract::Mammalian cumulus-oocyte complexes (COCs) reach full developmental capability during folliculogenesis and oogenesis. It is well recognized that only gametes achieving MII stage after in vivo or in vitro maturation (IVM) are successfully fertilized by a single spermatozoon. Although the process of oocyte nuclear and/or...
journal_title:DNA and cell biology
pub_type: 杂志文章
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journal_title:DNA and cell biology
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pub_type: 杂志文章,评审
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journal_title:DNA and cell biology
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:DNA and cell biology
pub_type: 杂志文章
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0637
更新日期:2007-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-07-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章,评审
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更新日期:2018-05-01 00:00:00
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pub_type: 临床试验,杂志文章
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
pub_type: 杂志文章
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pub_type: 杂志文章
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abstract::The gene encoding the mouse cellular retinoic acid-binding protein (CRABP) has been isolated from a mouse genomic library and its structure has been determined. This gene spans approximately 10.5 kb and consists of four exons encoding 24, 59, 38, and 16 amino acid residues, respectively. This gene structure is very si...
journal_title:DNA and cell biology
pub_type: 杂志文章
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