Abstract:
:The CAT gene is widely used as a reporter in eukaryotic systems because of the efficient translation of its mRNA. We report here that a sequence occurring in the CAT mRNA at +15 nucleotides from CAT AUG is essential for translation. This sequence includes a stem-loop structure, which, however, exhibits a calculated stability significantly lower than that required for a hairpin to act as an enhancer of translation in vitro. Replacement of this region with the corresponding sequence from mRNAs that are normally translated in eukaryotic systems drastically reduced translation of CAT in COS cells, although the consensus sequence around the AUG, known to be required for high-level translation initiation, was conserved. These observations may be relevant for the exploitation of the CAT reporter system for analysis of the mechanisms of translation initiation by means of fusion constructs.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Ciullo M,Del Pozzo G,Autiero M,Guardiola Jdoi
10.1089/104454900314690keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
39-46issue
1eissn
1044-5498issn
1557-7430journal_volume
19pub_type
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