Abstract:
:The human OZF gene encodes a protein consisting essentially of zinc finger motifs of the Krüppel type. Evolutionary studies revealed amplification of the bovine OZF gene in cattle. Domestic cattle includes two major subspecies: taurine (Bos primigenius taurus) and zebu (B. p. indicus). Amplified sequences were found in both subspecies and mapped to the same locus of chromosome X in band q11. No amplification was found in other bovids and in particular in Bison, the closest related genus to the genus Bos. One copy of the amplified locus was cloned and sequenced. It encodes a protein sharing 95% identity with the human OZF protein. The bovine OZF gene was detected in an additional locus on chromosome 18 in both Bos and Bison, in band q23-24, which is likely to represent the ancestral position of the OZF gene. This is the first evidence of gene amplification in a mammalian species during evolution.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Le Chalony C,Apiou F,Pibouin L,Dutrillaux B,Goubin Gdoi
10.1089/dna.1996.15.83subject
Has Abstractpub_date
1996-01-01 00:00:00pages
83-8issue
1eissn
1044-5498issn
1557-7430journal_volume
15pub_type
杂志文章abstract::Thioredoxin peroxidase (TPx) is an antioxidant protein that limits the activity of reactive oxygen species (ROS). We cloned the cDNA encoding the mouse homolog of TPx from an E14.5 brain cDNA library and analyzed its distribution and function in murine tissues. Comparison of the amino acid sequence of mouse TPx with t...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.311
更新日期:1997-03-01 00:00:00
abstract::The nuclear factor of κB (NFκB) family has been well known for its significant role in regulating the expression of numerous genes that control many biological processes. However, it is unclear whether NFκB could regulate milk synthesis. In this study, we identified NFκB1 as a critical regulator for milk synthesis in ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2016.3610
更新日期:2017-04-01 00:00:00
abstract::DNA condensed agents can improve the transfection efficiency of the cationic liposome delivery system. However, various condensed agents have distinct transfection efficiency and cellular cytotoxicity. The object of this study was to screen the optimal agents with the high transfection efficiency and low cytotoxicity ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4464
更新日期:2019-10-01 00:00:00
abstract::Electroporation has proven to be a highly effective technique for the in vivo delivery of genes to a number of solid tissues. In most of the reported methods, DNA is injected into the target tissue and electrodes are placed directly on or in the tissue for application of the electric field. While this works well for s...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454903322625000
更新日期:2003-12-01 00:00:00
abstract::Disruption of cell-matrix interactions can lead to anoikis-apoptosis due to loss of matrix contacts. We previously showed that Nerve/glial antigen 2 (NG2) is a novel anoikis receptor. Specifically, overexpression of NG2 leads to anoikis propagation, whereas its suppression leads to anoikis attenuation. Interestingly, ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2399
更新日期:2014-12-01 00:00:00
abstract::A safe and effective adjuvant is important to develop vaccines against highly pathogenic avian influenza virus. Chitosan, a derivative from the natural amino polysaccharide chitin, has been proved to be an effective adjuvant for inactivated influenza virus vaccine. In this study, protective immunity in mice provided b...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2009.0977
更新日期:2010-09-01 00:00:00
abstract::The overexpression of prosurvival members of the Bcl-2 family is commonly associated with the enhanced malignancy of hematological tumors. There has been great interest in a novel set of agents that are able to mimic the function of the BH3 domain by binding to the groove of Bcl-2-like proteins and initiating the cell...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0675
更新日期:2008-03-01 00:00:00
abstract::Endogenous phytohormones auxin (indole-3-acetic acid [IAA]), abscisic acid (ABA), gibberellin (GA3), and brassinosteroid (BR) play a role in responses to drought stress in higher plants. Tea plant is one of the major economic corps worldwide. The tender shoots of tea plants are the main source for tea production. The ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4896
更新日期:2019-11-01 00:00:00
abstract::Myocardial ischemic disease affects the prognosis in perioperative patients. Diabetes can aggravate myocardial injury. The purpose of this research is to investigate the effect of ferroptosis in the process of diabetes mellitus (DM) myocardial ischemia/reperfusion (I/R) injury (IRI). Endoplasmic reticulum stress (ERS)...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5097
更新日期:2020-02-01 00:00:00
abstract::The pathogenic strains of Vibrio cholerae that cause acute enteric infections in humans are derived from environmental nonpathogenic strains. To track the evolution of pathogenic V. cholerae and identify potential precursors of new pathogenic strains, we analyzed 324 environmental or clinical V. cholerae isolates for ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2008.0737
更新日期:2008-07-01 00:00:00
abstract::My 45 years of studying the molecular biology of adeno-associated virus are recounted. Additional activities as a mentor, department chair, and medical school administrator are described, as are my activities in the public sphere, which involved national issues related to science policy and medical education. ...
journal_title:DNA and cell biology
pub_type: 传,历史文章,杂志文章
doi:10.1089/dna.2013.2120
更新日期:2013-07-01 00:00:00
abstract::Most studies on the tissue distribution of actin isoform transcripts have been done in small mammals such as rat and mouse. We have begun a characterization of the actin gene family in a large mammal, the bovine. The alpha skeletal gene was isolated, and an isoform-specific probe to the 3' untranslated region of the t...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.555
更新日期:1995-06-01 00:00:00
abstract::Intestinal phospholipase A/lysophospholipase (IPAL) is an intestine-specific brush-border enzyme expressed during development and along the intestinal crypt-villus axis in a pattern similar to another well characterized brush-border enzyme, sucrase-isomaltase (SI). A tissue-specific DNase I hypersensitive site was ide...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.1419
更新日期:1997-12-01 00:00:00
abstract::Uterine corpus endometrial carcinoma (UCEC) is one of the leading causes of death from gynecological cancer due to the high recurrence rate. A recent study indicated that molecular biomarkers can enhance the recurrence prediction power if they were integrated with clinical information. In this study, we attempted to i...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5148
更新日期:2020-04-01 00:00:00
abstract::Fibulin-5 (FBLN-5) is a widely expressed, integrin-binding extracellular matrix protein that mediates endothelial cell adhesion and scaffolds cells to elastic fibers. It is also a gene target of TGF-beta in fibroblasts and endothelial cells that regulates cell proliferation and motility in a context-specific manner. W...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454904323145254
更新日期:2004-06-01 00:00:00
abstract::Heme plays a significant pathogenic role in several diseases involving the kidney. The cellular content of heme, derived either from the delivery of filtered heme proteins such as hemoglobin and myoglobin, or from the breakdown of ubiquitous intracellular heme proteins, is regulated via the heme oxygenase enzyme syste...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454902753759726
更新日期:2002-04-01 00:00:00
abstract::The PC12 pheochromocytoma cell line has been a favorite model system for cell and neurobiologists, but has proven relatively refractory to standard DNA transfection methods. We have found that the cationic lipid "lipofectin" provides a simple, gentle, and nontoxic procedure that vastly improves transfection efficienci...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.221
更新日期:1990-04-01 00:00:00
abstract::Sterol carrier protein2 (SCP2) is a 13.2-kD protein that is thought to be involved in the intracellular transport of cholesterol. Using synthetic oligonucleotides based on the protein sequence of SCP2, a clone (SP43) was isolated from a rat liver cDNA library. The DNA sequence revealed that the cDNA could encode a pol...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.159
更新日期:1990-04-01 00:00:00
abstract::Gene expression is modulated by epigenetic factors that come in varying forms, such as DNA methylation, histone modifications, microRNAs, and long noncoding RNAs. Recent studies reveal that these epigenetic marks are important regulatory factors in brain function. In particular, DNA methylation dynamics are found to b...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2011.1565
更新日期:2012-10-01 00:00:00
abstract::Denaturing gradient gel electrophoresis (DGGE) was used to examine error rates and mutations induced by native (wt) and exonuclease-deficient (exo-) Deep Vent DNA polymerases during DNA amplification by polymerase chain reaction (PCR), in the presence or absence of the T4 bacteriophage gene 32 protein (gp32).gp32 was ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.589
更新日期:1996-07-01 00:00:00
abstract::Methionine is the limiting amino acid for milk protein synthesis in dairy cows. The effect of methionine availability on milk protein synthesis is dependent on its active transport into cells through amino acid transporters. L-type amino acid transporter 1 (LAT1), which induces the transport of neutral amino acids, is...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3792
更新日期:2017-12-01 00:00:00
abstract::Sterol regulatory element-binding proteins (SREBPs) are transcription factors governing transcription of genes related to cholesterol and fatty acid metabolism. To become active, SREBPs must undergo a proteolytic cleavage to allow an active NH(2)-terminal segment to translocate into the nucleus. SKI-1/S1P is the first...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0624
更新日期:2007-11-01 00:00:00
abstract::The promoter of the human urokinase plasminogen activator (uPA) gene contains a sequence identical with the retinoblastoma control element (RCE) of the murine c-fos gene, as well as several Sp1 binding sites. In a number of cell lines, the uPA promoter is activated during enforced expression of the retinoblastoma prot...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.1063
更新日期:1994-11-01 00:00:00
abstract::Herpes simplex virus (HSV) is a neurotropic DNA virus with many favorable properties as a gene delivery vector. HSV is highly infectious, so HSV vectors are efficient vehicles for the delivery of exogenous genetic material to cells. Viral replication is readily disrupted by null mutations in immediate early genes that...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454902762053864
更新日期:2002-12-01 00:00:00
abstract::Traditional histological classification of lung cancer subtypes is informative, but incomplete. Recent studies of gene expression suggest that molecular classification can be used for effective diagnostic and prediction of the treatment outcome. We attempt to build a molecular classification based on the public data a...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2004.23.715
更新日期:2004-10-01 00:00:00
abstract::Triple-negative breast cancer (TNBC) refers to breast cancer without significant expression of estrogen receptor (ER), progesterone receptor (PR), or human epidermal growth factor receptor 2. We sought to identify the hub genes and find the potential progression mechanism of TNBC as well as immunotherapeutic targets. ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5449
更新日期:2020-10-01 00:00:00
abstract::We have devised a method to determine gene structure that utilizes the known gene structure of homologous proteins and the polymerase chain reaction (PCR). Because homologous proteins have evolved from a common ancestral gene, it is possible to design primers corresponding to the adjacent exon sequences in the protein...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.343
更新日期:1995-04-01 00:00:00
abstract::The potential association of variant surface glycoprotein (VSG) gene expression with clonal expression of virulence in African trypanosomes was addressed. Two populations of clonally related trypanosomes, which differ dramatically in virulence for the infected host, but display the same apparent VSG surface coat pheno...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2009.0991
更新日期:2010-05-01 00:00:00
abstract::A primary response of the avian intestine to 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] is increased synthesis of a 28-kD calcium-binding protein, calbindin-D28k (CaBP). This study examined whether 1,25-(OH)2D3 regulates CaBP gene transcription by an interaction of the vitamin D receptor (VDR) with a vitamin D-responsive...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.377
更新日期:1992-06-01 00:00:00
abstract::We have characterized four sequences from a small library containing a subset of the repetitive families of the mouse. Each clone has a repetition frequency of less than 1,100 copies per genome and each clone represents a unique family of middle repetitive DNA. One clone (pMR111) shares homology with mouse intracister...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.319
更新日期:1993-05-01 00:00:00