Abstract:
:A safe and effective adjuvant is important to develop vaccines against highly pathogenic avian influenza virus. Chitosan, a derivative from the natural amino polysaccharide chitin, has been proved to be an effective adjuvant for inactivated influenza virus vaccine. In this study, protective immunity in mice provided by chitosan-adjuvanted inactivated H5N1 vaccine was compared with that from an aluminum hydroxide-adjuvanted one. Mice were injected intraperitoneally once or twice with various dosages of inactivated vaccine alone or in combination with an adjuvant (chitosan or aluminum hydroxide). To test the immunization effect, mice were challenged with a lethal dose of H5N1 virus. The results showed that the adjuvanted vaccines were more effective than adjuvant-free ones in inducing humoral immune responses and protecting mice against lethal challenge. Chitosan was comparable to the alum adjuvant in efficacy. These findings indicated that chitosan might be a candidate adjuvant for parenteral administration of inactivated influenza vaccines.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Chang H,Li X,Teng Y,Liang Y,Peng B,Fang F,Chen Zdoi
10.1089/dna.2009.0977subject
Has Abstractpub_date
2010-09-01 00:00:00pages
563-8issue
9eissn
1044-5498issn
1557-7430journal_volume
29pub_type
杂志文章abstract::The centrosome is a unique organelle that functions as the microtubule organizing center in most animal cells. During cell division, the centrosomes form the poles of the bipolar mitotic spindle. In addition, the centrosomes are also needed for cytokinesis. Each mammalian somatic cell typically contains one centrosome...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/1044549041562276
更新日期:2004-08-01 00:00:00
abstract::Chromosomal gain on 1q23-24 is a cytogenetic finding found in approximately 30% of bladder tumors. Currently, no defined or candidate tumor-associated genes from this region have been identified. The objective of this study was to identify and quantitate the expression of putative cancer genes located at this chromoso...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902760599681
更新日期:2002-10-01 00:00:00
abstract::Design and synthesis of new small molecules binding to double-stranded RNA necessitate complete understanding of the molecular aspects of the binding of many existing molecules. Toward this goal, in this work we evaluated the biophysical aspects of the interaction of a DNA intercalator (proflavine) and a minor groove ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2008.0838
更新日期:2009-04-01 00:00:00
abstract::Ulcerative colitis (UC) is a long-time inflammatory condition arising from aberrant immune activation in the colon and the rectum. Interleukin (IL)-35 plays critical roles in autoimmune disorders. In this study, we explored the pathways of IL-35 in affecting UC. First, peripheral blood mononuclear cells (PBMCs) from U...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2016.3570
更新日期:2017-05-01 00:00:00
abstract::Duchenne muscular dystrophy is the most common and severe form of progressive muscular dystrophy. Previous results showed an increased survival in double knockout mice (dko) when treated with adipose-derived CD146+ cells. In this study, we analyzed the effect of CD146+ cells compared to mesenchymal stem/stromal cells ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4158
更新日期:2018-09-01 00:00:00
abstract::A human growth hormone expression construct was delivered intracellularly into the abdominal skin of mice by particle bombardment-mediated gene transfer. Using this technology, the in vivo delivery of antigen-encoding expression vectors affixed to gold microprojectiles results in de novo antigen production in target s...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.791
更新日期:1993-11-01 00:00:00
abstract::Transfection of keratinocytes with plasmid DNA leads to the loss of detectable DNA-binding activity of CCAAT displacement protein but not of Yin Yang 1, as monitored by electrophoretic mobility shift assay. This phenomenon was found to be attributable to the presence of plasmid DNA in the nuclear extracts prepared fro...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902753604989
更新日期:2002-02-01 00:00:00
abstract::The prototypic member of the transforming growth factor beta family is TGFbeta1, which is known to be important in extracellular matrix production, cell proliferation, and cell differentiation. Specifically in the pituitary lactotroph, TGFbeta1 inhibits prolactin (PRL) peptide secretion, PRL mRNA levels, and PRL gene ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999314863
更新日期:1999-11-01 00:00:00
abstract::Long noncoding RNAs (lncRNAs) may serve as potential molecular diagnostic markers to improve the capacity of earlier and more accurate diagnosis of dilated cardiomyopathy (DCM). We integrated five independent transcriptomic datasets (n = 504) from Gene Expression Omnibus for systematic identification of lncRNA-based d...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5670
更新日期:2020-11-03 00:00:00
abstract::The micronucleus (MN) assay in exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans exposed to occupational and environmental agents. The MN test is used as an indicator of genotoxic exposition, since it is associated with chromosome aberrations. An increased mutati...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2009.0996
更新日期:2010-06-01 00:00:00
abstract::Heterotrimeric G proteins, which are composed of Gα and Gβγ subunits, transduce signals sensed by the coupled surface receptors. Aberrant expressions of G proteins have been observed in many cancer types. This study aimed to determine the expression level of the stimulatory G protein alpha S subunit (Gαs, the main tra...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2016.3535
更新日期:2017-03-01 00:00:00
abstract::Interferon gamma (IFN-γ) is one of the key cytokines that plays a major role against viral and intracellular bacterial infection. In addition to the IFN-γ gene, teleost fish possess a second copy known as IFN-γ-related (IFN-γrel) gene. This report describes structural and functional properties of IFN-γrel gene in the ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2656
更新日期:2015-05-01 00:00:00
abstract::The activation of H-plk (Human-proviral linked Krüppel), a human Krüppel-related zinc finger gene in organs such as placenta, adrenal cortex, and testis, is probably due to insertion of an endogenous retrovirus, ERV3, upstream of the gene. Several differently spliced transcripts originate from this locus, e.g., a tran...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.27
更新日期:1998-01-01 00:00:00
abstract::The Egr-1 (zfp-6) gene encodes a zinc-finger-containing nuclear protein that is rapidly and transiently induced in quiescent cells treated with mitogens. We have constructed baculovirus vectors that synthesize mouse Egr-1 protein initiating at two putative ATG start sites. The ATG site producing the larger protein (Mr...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1991.10.61
更新日期:1991-01-01 00:00:00
abstract::Most mammalian species express high concentrations of ALDH3A1 in corneal epithelium with the exception of the rabbit, which expresses high amounts of ALDH1A1 rather than ALDH3A1. Several hypotheses that involve catalytic and/or structural functions have been postulated regarding the role of these corneal ALDHs. The ai...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454903322216671
更新日期:2003-05-01 00:00:00
abstract::A nomenclature system for the UDP glucuronosyltransferase superfamily is proposed, based on divergent evolution of the genes. A total of 26 distinct cDNAs in five mammalian species have been sequenced to date. Comparison of the deduced amino acid sequences leads to the definition of two families and a total of three s...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.1991.10.487
更新日期:1991-09-01 00:00:00
abstract::Mitochondrial FAD-linked glycerol-3-phosphate dehydrogenase (mtGPDH) is one of the two enzymes of the glycerol phosphate shuttle. This shuttle transfers reducing equivalents from the cytoplasm to the mitochondria in a unidirectional, exothermic manner. Here, the isolation and characterization of the rat nuclear gene (...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.301
更新日期:1998-03-01 00:00:00
abstract::Long noncoding RNAs (lncRNAs) play important roles in the development of human cancers. This is the first case-control study of the association between specific polymorphisms in lncRNA genes and the risk of lung cancer, as well as the gene-environment interaction between the polymorphisms and cooking oil fume exposure...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4200
更新日期:2018-08-01 00:00:00
abstract::Oxysterols are oxygenated derivatives of cholesterol. They have been shown to influence a variety of biological functions including sterol metabolism, lipid trafficking, and apoptosis. Recently, 12 human OSBP-related genes have been identified. In this study, we have identified a family of 12 oxysterol-binding protein...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902320308942
更新日期:2002-08-01 00:00:00
abstract::Traditional histological classification of lung cancer subtypes is informative, but incomplete. Recent studies of gene expression suggest that molecular classification can be used for effective diagnostic and prediction of the treatment outcome. We attempt to build a molecular classification based on the public data a...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2004.23.715
更新日期:2004-10-01 00:00:00
abstract::Osteosarcoma is one of the most common primary malignant tumors of the bone and tends to develop in teenage years. Although multitreatments for the diagnosis and therapy of osteosarcoma have been developed, there are still needs of new methods to prevent and treat the osteosarcoma. Here, we performed bioinformatic ana...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5377
更新日期:2020-07-01 00:00:00
abstract::The aim of this study is to discuss the hypothesis that expression of plasma atherosclerosis-associated microRNAs (miRNAs) in hyperhomocysteinemia (Hhcy) patients could predict the presence of atherosclerosis from different channels. Six plasma miRNAs (miR-145, miR-155, miR-222, miR-133, miR-217, and miR-30) selected ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3845
更新日期:2018-03-01 00:00:00
abstract::We have analyzed the interleukin-1 beta (IL-1 beta) promoter region near the cap site. Specific DNA sequences required for lipopolysaccharide (LPS) induction within this region were identified using transfection of reporter plasmids that contained portions of the proximal IL-1 beta 5'-flanking sequence. An LPS-respons...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.561
更新日期:1994-06-01 00:00:00
abstract::CXXC finger protein 1 (CFP1) is a component of the Setd1A and Setd1B methyltransferase complexes, localizes to euchromatic regions of the genome, and specifically binds unmethylated CpG dinucleotides in DNA. Murine embryos lacking CFP1 exhibit peri-implantation lethality, a developmental time that correlates with glob...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0714
更新日期:2008-10-01 00:00:00
abstract::The p53 tumor suppressor gene is frequently mutated in multiple human cancers, leading to loss of wild-type p53 (wt-p53)-dependent functions and tumorigenesis. p53 gene therapy is used to induce apoptosis in human cancer cells and tumors. Activation of nuclear factor kappa B (NF-kappaB) causes resistance to both chemo...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2008.0839
更新日期:2009-12-01 00:00:00
abstract::Our previous study showed that the mutation hotspots of the K-ras proto-oncogene in human functional adrenocortical tumors are in codons 15, 16, 18, and 31, thus differing from the sites in other tumors. In addition, analyzing the K-Ras protein by a recombinant DNA technique showed that the activity of endogenic GTPas...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901750219116
更新日期:2001-04-01 00:00:00
abstract::Aberrant neutrophil (PMN) infiltration of the intestinal mucosa is a hallmark of inflammatory bowel diseases, including Crohn's disease and ulcerative colitis. While the genotoxic function of PMNs and its implications in carcinogenesis have been primarily associated with oxidative stress, recent work by Butin-Israeli ...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2019.4842
更新日期:2019-08-01 00:00:00
abstract::Polyhomeotic is a member of the Polycomb group of genes. The products of this group are chromatin-associated proteins that act together as multimeric complexes. These proteins are required for the maintenance of target gene repression in a permanent and heritable manner during development. In order to better understan...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901316976118
更新日期:2001-08-01 00:00:00
abstract::The human ADH1A, ADH1B, and ADH1C genes encode alcohol dehydrogenases (ADHs) that metabolize ethanol. They evolved by recent tandem duplications and have similar proximal cis-acting elements, but differ in tissue-specificity. We hypothesized that distal cis-acting elements confer tissue-specificity. In this article, w...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902320908441
更新日期:2002-11-01 00:00:00
abstract::We have isolated and characterized a cDNA encoding a chicken beta homolog of c-erbA, or thyroid hormone receptor (TR). Chicken liver cDNA libraries were screened with a rat TR beta-1 cDNA probe, and several cDNA inserts were isolated and characterized. The sequence of one cDNA predicts a 369-amino-acid open reading fr...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1991.10.211
更新日期:1991-04-01 00:00:00