Abstract:
:Transfection of keratinocytes with plasmid DNA leads to the loss of detectable DNA-binding activity of CCAAT displacement protein but not of Yin Yang 1, as monitored by electrophoretic mobility shift assay. This phenomenon was found to be attributable to the presence of plasmid DNA in the nuclear extracts prepared from transfected cells. Treatment of these nuclear extracts with DNase I restored the ability to monitor DNA-binding activity of CDP. This report documents a new pitfall associated with transfection.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Narahari J,Roman Adoi
10.1089/104454902753604989keywords:
subject
Has Abstractpub_date
2002-02-01 00:00:00pages
109-13issue
2eissn
1044-5498issn
1557-7430journal_volume
21pub_type
杂志文章abstract::Acetate and β-hydroxybutyrate (BHBA) are the predominant substrates for de novo fatty acid (FA) synthesis in mammary gland of dairy cow. To investigate the nutrigenomic role of acetate and BHBA in bovine mammary epithelial cells during milk fat production, RNA sequencing (RNA-seq) transcriptomic analysis was used to i...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4783
更新日期:2020-03-01 00:00:00
abstract::The potential association of variant surface glycoprotein (VSG) gene expression with clonal expression of virulence in African trypanosomes was addressed. Two populations of clonally related trypanosomes, which differ dramatically in virulence for the infected host, but display the same apparent VSG surface coat pheno...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2009.0991
更新日期:2010-05-01 00:00:00
abstract::The promoter of the human urokinase plasminogen activator (uPA) gene contains a sequence identical with the retinoblastoma control element (RCE) of the murine c-fos gene, as well as several Sp1 binding sites. In a number of cell lines, the uPA promoter is activated during enforced expression of the retinoblastoma prot...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.1063
更新日期:1994-11-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is a life-threatening disease that is often associated with chronic infection by hepatitis B and C viruses. Genetic polymorphisms have been reported to alter the risk for HCC development. Genetic studies based on the haplotype have an increased power for detecting disease associations co...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2009.0921
更新日期:2009-11-01 00:00:00
abstract::The cDNAs encoding the human bone morphogenetic proteins BMP-2 and BMP-4 in an eukaryotic expression vector were permanently transferred into the murine mesenchymal progenitor cell line C3H10T1/2. Originally, these cells are known to differentiate into myotubes, adipocytes, and chondrocytes upon the addition of azacyt...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.871
更新日期:1993-12-01 00:00:00
abstract::The mammalian CYP2E1 gene encodes a cytochrome P450 enzyme that is markedly increased soon after birth. In vitro evidence suggests that the rat CYP2E1 gene is positively regulated by the transcription factor HNF-1 or a protein displaying DNA-binding properties similar to HNF-1. In contrast to newborn wild-type mice an...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.771
更新日期:1990-12-01 00:00:00
abstract::ERGIC2 (formerly known as PTX1) is a gene identified by subtractive hybridization on the basis that it is expressed in normal human prostate, but not in prostate carcinoma. It is unrelated to the gene encoding pituitary homeobox protein (Ptx1 or Pitx1), which regulates pituitary hormone gene expression. Based on seque...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2013.2225
更新日期:2014-02-01 00:00:00
abstract::Promoter elements located up to 2 kb upstream of the apolipoprotein A-I (apoA-I) gene are necessary for apoA-I expression in liver and intestine cells in tissue culture. In transgenic mice, a distal enhancer located between the apoA-IV and apoC-III genes is additionally necessary for tissue-specific expression of apoA...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315493
更新日期:1999-02-01 00:00:00
abstract::The expression of growth factors, proteolytic enzymes, fibrogenic factors, and cytokines is altered in the Helicobacter pylori-infected gastric mucosa. Therefore, we aimed to evaluate the association of functional promoter variants of transforming growth factor (TGF)-B1 and matrix metalloproteinase (MMP)-7 genes with ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2008.0842
更新日期:2009-06-01 00:00:00
abstract::Noncanonical transcriptions include transcriptions that systematically exchange nucleotides, also called bijective transformations or swinger transformations. Swinger transformation A↔T+C↔G recovers identities of 8 among 9 unknown RNAs differentially expressed in centroblastic lymphoma, a human immunodeficiency virus ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5066
更新日期:2020-08-01 00:00:00
abstract::Normal embryo development in oviparous (egg-laying) species requires the coordinated targeting to growing oocytes of nutrients and regulatory molecules such as retinol, the precursor of active retinoids. Serum retinol-binding protein (RBP) is the major carrier protein for retinol in the circulatory system of vertebrat...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.403
更新日期:1995-05-01 00:00:00
abstract::Apoptotic endonuclease G (EndoG) is responsible for DNA fragmentation both during and after cell death. Previous studies demonstrated that genetic inactivation of EndoG is cytoprotective against various pro-apoptotic stimuli; however, specific inhibitors for EndoG are not available. In this study, we have developed a ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2530
更新日期:2015-02-01 00:00:00
abstract::The present study was to explore whether the polymorphisms of FAM167 A-BLK region are associated with the susceptibility to autoimmune thyroid disease (AITD). The second sequencing technology was undertaken for seven tag loci mapping of the FAM167 A-BLK region, namely, rs11250144, rs2618431, rs4840568, rs13277113, rs2...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4344
更新日期:2018-11-01 00:00:00
abstract::Zidovudine (AZT) is widely used in the treatment of human immunodeficiency virus (HIV) infection because of its ability to act as a false substrate for the viral reverse transcriptase. AZT in the clinical setting has also been shown to produce significant hematologic and other toxicity, even though prior reports in th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.363
更新日期:1996-05-01 00:00:00
abstract::The liver has an excellent capacity for regeneration when faced with external injury and the damage differs from that of other organs in the body. Our aim was to identify the role of circular RNA (circRNA) during the DNA synthesis phase (36 h) of mice liver regeneration. High-throughput RNA sequencing was conducted to...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5750
更新日期:2020-11-01 00:00:00
abstract::Mitochondrial aldehyde dehydrogenase-2 (ALDH2) is the principal enzyme involved in alcohol metabolism in humans. Emerging evidence has shown that the common rs671 G>A (Glu504Lys) polymorphism in the ALDH2 gene might play a critical role in increasing the susceptibility to coronary heart disease (CHD), including myocar...
journal_title:DNA and cell biology
pub_type: 杂志文章,meta分析
doi:10.1089/dna.2013.1995
更新日期:2013-07-01 00:00:00
abstract::Glucose-stimulated insulin gene transcription is mainly regulated by a 340-bp promoter region upstream of the transcription start site by beta-cell-enriched transcription factors Pdx-1, MafA, and NeuroD1. Previous studies have shown that histone H4 hyperacetylation is important for acute up-regulation of insulin gene ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1336
更新日期:2012-01-01 00:00:00
abstract::Diabetes is a worldwide health problem with increasing prevalence. Some reports indicate the interplay between bone and glucose metabolism. The imbalance between bone resorption and formation resulted in the structural integrity and strength of bone. Glucagon-like peptide-1 (GLP-1) and its agonists (Liraglutide) have ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4397
更新日期:2018-12-01 00:00:00
abstract::We have determined the expression pattern of all 11 human replication-dependent histone H3 genes in three fetal human tissues (bladder, liver, and lung) and in eight human cell lines by RNase protection assay. In the fetal human tissues, all 11 genes were expressed to a varied extent. However, the relative contributio...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454903321908629
更新日期:2003-04-01 00:00:00
abstract::Cytochrome P450c17 catalyzes the 17alpha-hydroxylase/17,20 lyase activity needed for sex steroid synthesis. We recently characterized the nuclear phosphoprotein SET as a novel transcriptional regulator that binds to the -447/-399 region of the rat P450c17 gene, along with the transcription factors COUP-TF II, NGF-IB, ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901753340604
更新日期:2001-10-01 00:00:00
abstract::G0S8 is a member of a set of putative G0/G1 switch regulatory genes (G0S genes) selected by screening cDNA libraries prepared from blood mononuclear cells cultured for 2 hr with lectin and cycloheximide. Comparison of a full-length cDNA sequence with the corresponding genomic sequence reveals an open reading frame of ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.125
更新日期:1994-02-01 00:00:00
abstract::Rheumatoid arthritis (RA) is a complex polygenic inflammatory disease associated with accelerated atherosclerosis, which is the main cause of increased cardiovascular (CV) morbidity and mortality in RA patients. CARD8 is a constituent of inflammasome, which regulates interleukin 1-beta production, and has been associa...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2012.1836
更新日期:2013-01-01 00:00:00
abstract::Long noncoding RNA (lncRNA) was closely attached to various cancers according to previous studies. In this study we aimed to investigate an lncRNA signature with prognostic value of over survival (OS) outcomes of gastric cancer (GC). Profiles of mRNAs expression and clinical information of 381 GC tissues and 32 nontum...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4832
更新日期:2019-09-01 00:00:00
abstract::Administration of endotoxin (LPS) in humans results in profound physiological responses, including activation of peripheral blood mononuclear cells and the release of inflammatory factors. The time course of the response of selected inflammatory proteins was examined in healthy subjects (n = 6) administered a single i...
journal_title:DNA and cell biology
pub_type: 临床试验,杂志文章
doi:10.1089/dna.2005.24.410
更新日期:2005-07-01 00:00:00
abstract::Interleukin-6 (IL-6) signaling may play a causal role in the development of coronary heart disease. However, the relationship between IL-6 genotypes and plasma levels of IL-6 appears to be complex. To help clarify the inconsistent findings, we conducted a meta-analysis of the published genetic association studies of t...
journal_title:DNA and cell biology
pub_type: 杂志文章,meta分析
doi:10.1089/dna.2013.2148
更新日期:2013-11-01 00:00:00
abstract::Extracellular vesicles classified as exosomes, microvesicles, or apoptotic bodies based on size are shed from most cells under normal as well as pathological conditions. They are released into the surrounding milieu, including plasma, urine, saliva, and tissues. Exosomes are highly enriched in microRNAs (miRs), which ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2015.2884
更新日期:2015-07-01 00:00:00
abstract::Chlamydial infection of the lower genital tract usually spreads to the upper genital tract and is then responsible for more serious consequences, such as infertility, ectopic pregnancy, pelvic pain, and pelvic inflammatory disease. Genital infection with Chlamydia trachomatis and the resulting cytokine response largel...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1333
更新日期:2012-03-01 00:00:00
abstract::Ginsenoside, a natural triterpenoid saponin, exhibits immunomodulatory and anticancer activities. In the present study, we demonstrated that ginsenoside Rg1 induced secretion of cytokines, including interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), and IL-1β, and chemokines such as IL-8 and IP-10 in a dose-depe...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3923
更新日期:2017-12-01 00:00:00
abstract::Global antibiotic resistance, driven by intensive antibiotic exposure/abuse, constitutes a serious challenge to all health care, particularly in an era when new antimicrobial development has slowed to a trickle. Recently, we published work demonstrating the discovery and partial mechanism of action of a novel bacteric...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2020.5824
更新日期:2020-09-01 00:00:00
abstract::The pathogenic strains of Vibrio cholerae that cause acute enteric infections in humans are derived from environmental nonpathogenic strains. To track the evolution of pathogenic V. cholerae and identify potential precursors of new pathogenic strains, we analyzed 324 environmental or clinical V. cholerae isolates for ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2008.0737
更新日期:2008-07-01 00:00:00