Abstract:
:The gene encoding the mouse cellular retinoic acid-binding protein (CRABP) has been isolated from a mouse genomic library and its structure has been determined. This gene spans approximately 10.5 kb and consists of four exons encoding 24, 59, 38, and 16 amino acid residues, respectively. This gene structure is very similar to the structures of other related genes belonging to the same protein family such as the human cellular retinol-binding protein, the rat cellular retinol-binding protein II, the rat fatty acid-binding protein, and the mouse adipocyte P2 protein. The site for transcription initiation has been mapped to the 93rd nucleotide upstream from the translation initiation codon ATG using both primer extension and RNase protection assays. From the DNA sequence, the promoter of the CRABP gene resembles those found in the "housekeeping" genes in that it is very G/C rich, lacks a TATA box, and contains multiple copies of the sequence GGGCGG. The deduced amino acid sequence of the translated region is identical to the amino acid sequence of the known bovine CRABP, and the DNA sequence of the transcribed region from the mouse gene shows approximately 78% homology to that of the bovine cDNA.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Wei LN,Tsao JL,Chu YS,Jeannotte L,Nguyen-Huu MCdoi
10.1089/dna.1990.9.471subject
Has Abstractpub_date
1990-09-01 00:00:00pages
471-8issue
7eissn
1044-5498issn
1557-7430journal_volume
9pub_type
杂志文章abstract::Palindromati, the massive host-edited synthetic palindromic contamination found in GenBank, is illustrated and exemplified. Millions of contaminated sequences with portions or tandems of such portions derived from the ZAP adaptor or related linkers are shown (1) by the 12-bp sequence reported elsewhere, exon Xb, 5' CC...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1339
更新日期:2012-02-01 00:00:00
abstract::Cytochrome P450 15 beta is a female-specific gene product that catalyzes the hydroxylation of steroid sulfates at the 15 beta position. Isolation and analysis of the gene for rat P450 15 beta reveals nine coding exons and encompasses more than 35 kb of chromosomal DNA. The intron-exon junctions are at similar position...
journal_title:DNA and cell biology
pub_type: 杂志文章
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abstract::Growth factors are important mediators of proliferation. Deregulation in growth factor mechanisms as well as in its receptors will contribute to cancer development. One of the most important is the epidermal growth factor (EGF), which is encoded by EGF gene. A functional polymorphism at position 61 (A/G) is associated...
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abstract::By using the methodology of both wet and dry biology (i.e., RT-PCR and cycle sequencing, and biocomputational technology, respectively) and the data obtained through the Genome Projects, we have cloned Xenopus laevis SOD2 (MnSOD) cDNA and determined its nucleotide sequence. These data and the deduced protein primary s...
journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:2017-06-01 00:00:00
abstract::The aims of our research involved to investigate DYS-14 copy number variations in healthy males, to quantify extracellular DNA in maternal circulation in normal versus complicated pregnancies, and to study variations in the DYS-14 copy number in extracellular male fetal DNA. Fifty-five healthy males, 43 uncomplicated ...
journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:2009-07-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490150504666
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.527
更新日期:1993-07-01 00:00:00
abstract::Regenerative medicine is a new interdisciplinary field in biomedical science, which aims at the repair or replacement of the defective tissue or organ by congenital defects, age, injury, or disease. Various cell-related techniques such as stem cell-based biotherapy are a hot topic in the current press, and stem cell r...
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pub_type: 杂志文章,评审
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更新日期:2013-10-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) production is regulated by growth factors and inflammatory cytokines, and VEGF plays a role in migraine. We examined for the first time whether three functional polymorphisms in the promoter region of VEGF gene (C(-2578)A, G(-1154)A, and G(-634)C) and VEGF haplotypes are assoc...
journal_title:DNA and cell biology
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更新日期:2010-07-01 00:00:00
abstract::Sepsis is an inflammatory-related disease, and severe sepsis would induce multiorgan dysfunction, which is the most common cause of death of patients in noncoronary intensive care units. Progression of novel therapeutic strategies has proven to be of little impact on the mortality of severe sepsis, and unfortunately, ...
journal_title:DNA and cell biology
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doi:10.1089/dna.2017.3944
更新日期:2018-02-01 00:00:00
abstract::A primary response of the avian intestine to 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] is increased synthesis of a 28-kD calcium-binding protein, calbindin-D28k (CaBP). This study examined whether 1,25-(OH)2D3 regulates CaBP gene transcription by an interaction of the vitamin D receptor (VDR) with a vitamin D-responsive...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.377
更新日期:1992-06-01 00:00:00
abstract::The prototypic member of the transforming growth factor beta family is TGFbeta1, which is known to be important in extracellular matrix production, cell proliferation, and cell differentiation. Specifically in the pituitary lactotroph, TGFbeta1 inhibits prolactin (PRL) peptide secretion, PRL mRNA levels, and PRL gene ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999314863
更新日期:1999-11-01 00:00:00
abstract::Transfection of keratinocytes with plasmid DNA leads to the loss of detectable DNA-binding activity of CCAAT displacement protein but not of Yin Yang 1, as monitored by electrophoretic mobility shift assay. This phenomenon was found to be attributable to the presence of plasmid DNA in the nuclear extracts prepared fro...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902753604989
更新日期:2002-02-01 00:00:00
abstract::The sex steroid hormone progesterone modulates the developmental and lactogenic activity of prolactin in the mammary gland. Regulation of the level of prolactin receptor (PRLR) provides one possible mechanism by which this may occur, prompting this investigation of the molecular mechanisms involved in progestin regula...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.721
更新日期:1992-12-01 00:00:00
abstract::This research aims to investigate the immune-associated gene signature from databases to improve the prognostic value in hepatocellular carcinoma (HCC) by multidimensional methods using various bioinformatic methods. Fifty-one immune-associated genes were mined out, which were associated with clinical characters throu...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2019.5099
更新日期:2020-04-01 00:00:00
abstract::In a systematic approach to identify interferon-gamma (IFN-gamma)-regulated host effector molecules, we found several members of the 65 kDa guanylate-binding proteins (GBPs) highly upregulated. During extensive characterization of these guanosine triphosphatases (GTPases), we identified discrepancies between the clone...
journal_title:DNA and cell biology
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更新日期:2007-12-01 00:00:00
abstract::Dilated cardiomyopathy (DCM) is a common type of cardiomyopathy. The pathogenesis of DCM remains unclear and involves varied genes. AXIN1 is a crucial gene in regulating various functions in cells, it encodes protein Axin1, which regulates the assembly and disassembly of β-catenin destruction complex. In addition, Wnt...
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abstract::CYP24A1 is overexpressed in colorectal cancer, and the reason for the dysregulation of CYP24A1 in colorectal cancer is still unknown. In the present study, experiments were designed to test whether CYP24A1 inhibition facilitated the antiproliferative effect of 1,25(OH)2D3. In addition, the role of methylation in the r...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.4058
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abstract::Dengue virus serotype 3 (DENV-3) was associated with severe dengue epidemics in Thailand during 1973-1999. We studied Thai DENV-3 viruses isolated from hospitalized children in Bangkok with differing disease severity during that period. Viruses were sequenced at their 5' and 3' untranslated regions (UTRs), which are r...
journal_title:DNA and cell biology
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doi:10.1089/dna.2008.0835
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abstract::Gene expression is modulated by epigenetic factors that come in varying forms, such as DNA methylation, histone modifications, microRNAs, and long noncoding RNAs. Recent studies reveal that these epigenetic marks are important regulatory factors in brain function. In particular, DNA methylation dynamics are found to b...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2011.1565
更新日期:2012-10-01 00:00:00
abstract::Aberrant neutrophil (PMN) infiltration of the intestinal mucosa is a hallmark of inflammatory bowel diseases, including Crohn's disease and ulcerative colitis. While the genotoxic function of PMNs and its implications in carcinogenesis have been primarily associated with oxidative stress, recent work by Butin-Israeli ...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2019.4842
更新日期:2019-08-01 00:00:00
abstract::CD95 (Fas/APO-1)-mediated apoptosis appears to be regulated by positive and negative signaling molecules. A human CD40/CD95 chimeric receptor was stably transfected into CD95-expressing human Jurkat T cells, and signaling through native and chimeric CD95 was compared in the same cell type to assess contributions of th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.197
更新日期:1997-02-01 00:00:00
abstract::Xenotransplantation from pigs to human beings is viewed as a potential solution for the acute organ shortage. However, consequent xenorejection induced by Gal alpha 1,3 Gal (a Gal, Gal antigen) prevents xenotransplantation from clinical application. Thus, the most attracting attempt to prevent xenorejection is the eli...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.235
更新日期:2005-04-01 00:00:00
abstract::The family of G protein-coupled receptors (GPCRs) serves as the target for almost a third of currently marketed drugs, and provides the predominant mechanism through which extracellular factors transmit signals to the cell. The discovery of GPCRs with no known ligand has initiated a frenzy of research, with the aim of...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2005.24.54
更新日期:2005-01-01 00:00:00
abstract::Most studies on the tissue distribution of actin isoform transcripts have been done in small mammals such as rat and mouse. We have begun a characterization of the actin gene family in a large mammal, the bovine. The alpha skeletal gene was isolated, and an isoform-specific probe to the 3' untranslated region of the t...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.555
更新日期:1995-06-01 00:00:00
abstract::Administration of endotoxin (LPS) in humans results in profound physiological responses, including activation of peripheral blood mononuclear cells and the release of inflammatory factors. The time course of the response of selected inflammatory proteins was examined in healthy subjects (n = 6) administered a single i...
journal_title:DNA and cell biology
pub_type: 临床试验,杂志文章
doi:10.1089/dna.2005.24.410
更新日期:2005-07-01 00:00:00