Abstract:
:This research aims to investigate the immune-associated gene signature from databases to improve the prognostic value in hepatocellular carcinoma (HCC) by multidimensional methods using various bioinformatic methods. Fifty-one immune-associated genes were mined out, which were associated with clinical characters through univariate and multivariate Cox regression analyses, and 51 immune-associated genes could be well-divided HCC samples into high-risk and low-risk clusters. Next, we performed least absolute shrinkage and selection operator (LASSO) Cox regression method to reveal 18 immune-associated genes' signature and calculate risk score of each gene for receiver operating characteristic (ROC) analysis. Comparing with low-risk cluster, high-risk cluster had higher risk score with unfavorable prognosis. Then, multivariate Cox regression analysis showed that risk score of 18 immune-associated genes' signature was associated with tumor invasion and tumor-node-metastasis (TNM) stage. ROC analysis indicated combined TNM stage, and risk score performed more sensitive and specific than single TNM stage or risk score in survival prediction. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis found that the pathways enriched in tumorigenesis were related to risk score, and those pathways could separate HCC samples into high and low clusters. In addition, the survival prediction of 18 immune-associated genes' signature was well validated in independent test data set, external data set, and Real-time Quantitative PCR (RT-qPCR) experiment. The 18 immune-associated genes' signature was constructed, which could be used in effective prediction of HCC prognosis.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Chen W,Tang D,Ou M,Dai Ydoi
10.1089/dna.2019.5099subject
Has Abstractpub_date
2020-04-01 00:00:00pages
499-512issue
4eissn
1044-5498issn
1557-7430journal_volume
39pub_type
杂志文章,评审abstract::Diabetes is a worldwide health problem with increasing prevalence. Some reports indicate the interplay between bone and glucose metabolism. The imbalance between bone resorption and formation resulted in the structural integrity and strength of bone. Glucagon-like peptide-1 (GLP-1) and its agonists (Liraglutide) have ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4397
更新日期:2018-12-01 00:00:00
abstract::Traditional histological classification of lung cancer subtypes is informative, but incomplete. Recent studies of gene expression suggest that molecular classification can be used for effective diagnostic and prediction of the treatment outcome. We attempt to build a molecular classification based on the public data a...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2004.23.715
更新日期:2004-10-01 00:00:00
abstract::The major surface glycoprotein (MSG) of Pneumocystis carinii is encoded by a family of related but distinct genes distributed throughout the P. carinii genome. Previous reports of the genomic and mRNA MSG structure suggested that there was a highly conserved 5'-untranslated region and a highly variable translated regi...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.989
更新日期:1996-11-01 00:00:00
abstract::The cDNAs encoding the human bone morphogenetic proteins BMP-2 and BMP-4 in an eukaryotic expression vector were permanently transferred into the murine mesenchymal progenitor cell line C3H10T1/2. Originally, these cells are known to differentiate into myotubes, adipocytes, and chondrocytes upon the addition of azacyt...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.871
更新日期:1993-12-01 00:00:00
abstract::Heme plays a significant pathogenic role in several diseases involving the kidney. The cellular content of heme, derived either from the delivery of filtered heme proteins such as hemoglobin and myoglobin, or from the breakdown of ubiquitous intracellular heme proteins, is regulated via the heme oxygenase enzyme syste...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454902753759726
更新日期:2002-04-01 00:00:00
abstract::We have further characterized the most distal of the three alpha-fetoprotein (AFP) enhancers required for expression of the AFP gene in fetal hepatocytes and yolk sac endodermal cells. Almost total rat AFP enhancer 3 (E3) activity is driven by a 160-bp fragment at -6 kb containing three target regions for nuclear prot...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.1063
更新日期:1996-12-01 00:00:00
abstract::CD36, a surface membrane glycoprotein, functions as a class B scavenger receptor that binds to several distinct ligands. Included among these is oxidized low-density lipoprotein (Ox-LDL), a major trigger of atherosclerotic lesions, and the levels of CD36 activity and Ox-LDL uptake may have an impact on coronary artery...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.25.302
更新日期:2006-05-01 00:00:00
abstract::There is strong evidence for the presence of a functional renin-angiotensin system in human adipose tissue. The aim of our study was to investigate the association of polymorphic variants of angiotensin-converting enzyme gene (ACE I/D) and angiotensin II type I receptor gene (AGTR1 A1166C) with extreme obesity and obe...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2013.2014
更新日期:2013-08-01 00:00:00
abstract::Cytochrome P450 15 beta is a female-specific gene product that catalyzes the hydroxylation of steroid sulfates at the 15 beta position. Isolation and analysis of the gene for rat P450 15 beta reveals nine coding exons and encompasses more than 35 kb of chromosomal DNA. The intron-exon junctions are at similar position...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.49
更新日期:1990-01-01 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is a malignancy with relatively high incidence and poor prognosis. RNA-binding proteins (RBPs) were reported to be dysregulated in multiple cancers and were closely associated with tumor initiation and progression. However, an integrated analysis of the roles of RBPs in HN...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5641
更新日期:2020-10-01 00:00:00
abstract::Age-related macular degeneration (AMD) is a disease with multifactorial etiology characterized by irreversible loss of central visual acuity. The discovery of susceptive single-nucleotide polymorphisms (SNPs) has progressed our understanding of AMD. Complement factor H (CFH) gene Y402H polymorphism and high-temperatur...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1214
更新日期:2012-03-01 00:00:00
abstract::Epulopiscium sp. type B, a member of the Firmicutes, is a large (up to 300 microm), cigar-shaped bacterial symbiont of surgeonfish that propagates itself by forming multiple intracellular offspring. This unusual form of reproduction is an apparent modification of a developmental program used by some Firmicutes to prod...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2008.0801
更新日期:2009-02-01 00:00:00
abstract::We previously showed that the human AKNA gene encodes an AT-hook transcription factor that regulates the expression of costimulatory cell surface molecules on lymphocytes. However, AKNA cDNA probes hybridize with multiple transcripts, suggesting either the existence of other homologous genes or a complex regulation op...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.325
更新日期:2005-05-01 00:00:00
abstract::Microsomal transfer protein (MTP), a lipid transfer protein localized in the endoplasmic reticulum of hepatocytes and enterocytes, plays an important role in the development of nonalcoholic fatty liver disease (NAFLD). Many existing studies have demonstrated that a common polymorphism (-493G>T, rs1800591 G>T) in the M...
journal_title:DNA and cell biology
pub_type: 杂志文章,meta分析
doi:10.1089/dna.2013.2238
更新日期:2014-06-01 00:00:00
abstract::Plastins (or fimbrins) are a family of actin-binding proteins that are conserved from yeast to humans. In mammals, three tissue-specific plastin isoforms have been identified. The L isoform (L-plastin) is normally expressed only in leukocytes but is also found in >90% of neoplastic nonleukocyte human cells. Because L-...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.9
更新日期:1997-01-01 00:00:00
abstract::Porifera (sponges) constitute the lowest metazoan phylum. Experiments examined whether sponges can recognize self/nonself molecules. Cells from the marine sponge Suberites domuncula were incubated with membranes from either S. domuncula or another marine sponge, Geodia cydonium, as well as with recombinant alpha-integ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999314746
更新日期:1999-12-01 00:00:00
abstract::A nomenclature system for the UDP glucuronosyltransferase superfamily is proposed, based on divergent evolution of the genes. A total of 26 distinct cDNAs in five mammalian species have been sequenced to date. Comparison of the deduced amino acid sequences leads to the definition of two families and a total of three s...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.1991.10.487
更新日期:1991-09-01 00:00:00
abstract::Sufficient levels of gene expression are required for effective gene therapy. One of the major obstacles in gene therapy is the low transgene expression obtained from currently available vector systems. To address this issue, we employed a transcriptional amplifier strategy in a single construct to enhance transgene e...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.595
更新日期:2005-09-01 00:00:00
abstract::We have cloned and sequenced the cDNA corresponding to the rat preproepidermal growth factor (ppEGF) mRNA. The cDNA contained 4,801 nucleotides, similar to that reported for the mouse (4,749 nucleotides) and the human mRNAs (4,871 nucleotides). The predicted protein sequence would contain 1,133 amino acids, smaller th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.481
更新日期:1992-07-01 00:00:00
abstract::Our aim was to evaluate in a cohort of 125 Italian patients with type 2 diabetes (T2D), who underwent neurological evaluation, the possible differences in the number of mitochondrial DNA copies (mtDNA) comparing positive and negative patients for cardiovascular autonomic neuropathy (CAN) or diabetic peripheral neuropa...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5326
更新日期:2020-08-01 00:00:00
abstract::The aromatic hydrocarbon receptor (AHR) is a ligand-activated transcription factor that regulates the expression of several drug-metabolizing enzymes and has been implicated in immunosuppression, teratogenesis, cell-specific hyperplasia, and certain types of malignancies and toxicities. The mouse Ahr gene 5' proximal ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.811
更新日期:1998-09-01 00:00:00
abstract::Most of the signal pathways involved in ultraviolet (UV)-induced skin carcinogenesis are thought to originate at plasma membrane receptors. However, UVA-induced signal transduction to downstream ribosomal protein S6 kinases, p70(S6K) and p90(RSK), is not well understood. In this report, we show that UVA stimulation of...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901753438589
更新日期:2001-12-01 00:00:00
abstract::Non-Hodgkin lymphoma (NHL) is the most common hematologic malignancy worldwide. Interleukin-2 (IL-2) plays a key role in the proliferation of T cells and natural killer cells. It has been reported that polymorphisms in the IL-2 gene are associated with various cancers. The aim of this study was to examine the effect o...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1603
更新日期:2012-07-01 00:00:00
abstract::RUNX2 is a member of the RUNX family of transcription factors, also containing the RUNX1 and RUNX3 proteins. These factors control the expression of genes essential for proper development in many cell lineages. RUNX2 plays a crucial role in the proliferation and differentiation of osteoblasts, required for bone format...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2014.2688
更新日期:2015-05-01 00:00:00
abstract::The incidence of premature ovarian failure (POF), a condition causing amenorrhea and hypergonadotropic hypoestrogenism in women before the age of 40, has been increasing in recent years. As an irreversible pathological change, improved treatment strategies for this disease are urgently needed. In this study, a type of...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2013.2032
更新日期:2013-12-01 00:00:00
abstract::Mouse vimentin intermediate filaments (IFs) reconstituted in vitro were analyzed for their capacity to select certain DNA sequences from a mixture of about 500-bp-long fragments of total mouse genomic DNA. The fragments preferentially bound by the IFs and enriched by several cycles of affinity binding and polymerase c...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.209
更新日期:1996-03-01 00:00:00
abstract::Rat cytokine-induced neutrophil chemoattractant (CINC) is an 8-kD polypeptide originally purified from media conditioned by interleukin-1 beta (IL-1 beta)-stimulated 52E, an epithelioid clone derived from the normal rat kidney (NRK) cell line. Using a fibroblastic clone of NRK cells, 49F, we found that lipopolysacchar...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.883
更新日期:1994-09-01 00:00:00
abstract::In this study, we explored the genes genetically associated with lupus nephritis (LN), and their function by bioinformatics analysis. We collected genes potentially associated with LN from National Center for Biotechnology Information Center (NCBI-Gene) and Online Mendelian Inheritance in Man (OMIM) databases. The maj...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4631
更新日期:2019-07-01 00:00:00
abstract::This study aimed to investigate the expression and clinical significance of chemokine-like factor-like MARVEL transmembrane domain-containing family member 2 (CMTM2) in hepatocellular carcinoma (HCC) tissues. Bioinformatics analysis showed that CMTM2 was downregulated in HCC tissues and correlated with vascular invasi...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5237
更新日期:2020-04-01 00:00:00
abstract::A series of overlapping cDNAs coding for mouse prothrombin (coagulation factor II) have been isolated and the composite DNA sequence has been determined. The complete prothrombin cDNA is 1,987 bp in length [excluding the poly(A) tail] and codes for 18 bp of 5' untranslated sequence, an open reading frame coding for 61...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.487
更新日期:1990-09-01 00:00:00