Abstract:
:A variety of human cancer cells are resistant to Fas ligand and anti-Fas antibody induced apoptosis. Previously, we reported that human gastric carcinoma cell lines were resistant to the anti-Fas antibody, CH-11, without interferon-gamma pretreatment in vitro. Cyclooxygenase (COX)-2 is known to be expressed in many human malignancies, and is correlated with tumor progression and resistance to apoptosis. This study examined whether NS398, a COX-2 inhibitor, inhibited cell proliferation and increased Fas-mediated apoptosis in human gastric carcinoma cell lines. Treatment of NS398 inhibited cell proliferation in MKN-45, which expressed the highest level of COX-2 among seven human gastric carcinoma cell lines, in a dose- and time-dependent manner, in contrast to less prominent effects in KATO-III, which expresses no COX-2. Although the treatment of CH-11 induced apoptosis in both cells, the simultaneous treatment of NS398 and CH-11 remarkably induced apoptosis, as confirmed by Hoechst 33258 staining and the terminal deoxynucleotidyl transferase- mediated dUTP-digoxigenin nick-end labeling (TUNEL) method in MKN-45. Flow cytometric analysis also revealed the increased pre-G1 fraction by the simultaneous treatment. The treatment of NS398 induced upregulation of Bad and PTEN, and downregulation of phosphorylated Akt (Thr308). These findings suggest that COX-2 might inhibit Fas-mediated apoptosis in human gastric carcinoma cell lines, especially MKN-45, by modulating PTEN and Akt.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Honjo S,Osaki M,Ardyanto TD,Hiramatsu T,Maeta N,Ito Hdoi
10.1089/dna.2005.24.141keywords:
subject
Has Abstractpub_date
2005-03-01 00:00:00pages
141-7issue
3eissn
1044-5498issn
1557-7430journal_volume
24pub_type
杂志文章abstract::Insulin-like growth factor I (IGF-I) plays a major role in development and metabolism. Currently, the cDNA-derived primary structure of IGF-I is known for some mammals and for chicken, frog, and salmon. Additionally, the organization of the human, rat, and chicken IGF-I genes has been established. The investigation of...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.729
更新日期:1993-10-01 00:00:00
abstract::We have shown previously that rat hepatoma FTO-2B cells express two mRNAs, called F (fetal) and L (liver), from distinct promoters of the same gene coding for 6-phosphofructo-2-kinase (PFK-2). This enzyme catalyzes the synthesis of fructose 2,6-bisphosphate, an allosteric stimulator of glycolysis. We have now found th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.1075
更新日期:1997-09-01 00:00:00
abstract::Several studies have revealed that miR-205 plays important roles in the development of gynecological cancers and thus may serve as a potential prognostic biomarker, but the current conclusions remain controversial. Therefore, the goal of this study was to explore the prognostic significance and functional mechanisms o...
journal_title:DNA and cell biology
pub_type: 杂志文章,meta分析
doi:10.1089/dna.2019.5316
更新日期:2020-05-01 00:00:00
abstract::Ginsenoside, a natural triterpenoid saponin, exhibits immunomodulatory and anticancer activities. In the present study, we demonstrated that ginsenoside Rg1 induced secretion of cytokines, including interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), and IL-1β, and chemokines such as IL-8 and IP-10 in a dose-depe...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3923
更新日期:2017-12-01 00:00:00
abstract::A decrease in gap junctional, intercellular communication (GJIC) has been associated with cells neoplastically transformed by a variety of factors. To investigate the role of the Ras oncogene product in gap junction function, a panel of murine C3H10T1/2 (10T1/2) fibroblasts was constructed in which the levels of ras g...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.443
更新日期:1996-06-01 00:00:00
abstract::The polB gene encodes DNA polymerase II in Escherichia coli. The nucleotide sequence shows an open reading frame of 2,304 nucleotides coding for a protein of 88 kD. The protein initiation signal is preceded by a lexA box lying 2 nucleotides from the termination signal of araD, and begins with GUG 75 nucleotides after ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.631
更新日期:1990-11-01 00:00:00
abstract::We have screened an Aplysia atrial gland cDNA library using a prohormone convertase (PC)1 probe prepared by polymerase chain reaction (PCR) and have isolated an Aplysia PC1-related full-length 3.6-kb cDNA clone. The cDNA sequence (3,565 bp) encoded a putative preproendoprotease (APC1) of 703 amino acid residues that s...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.339
更新日期:1996-04-01 00:00:00
abstract::Uterine corpus endometrial carcinoma (UCEC) is a common malignancy in the female reproductive system, associated with high morbidity and mortality. Despite the high prevalence of UCEC, molecular understanding of uterine endothelium tumorigenesis remains poorly understood. In this study, we reported that transcription ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.4062
更新日期:2018-05-01 00:00:00
abstract::Denaturing gradient gel electrophoresis (DGGE) was used to examine error rates and mutations induced by native (wt) and exonuclease-deficient (exo-) Deep Vent DNA polymerases during DNA amplification by polymerase chain reaction (PCR), in the presence or absence of the T4 bacteriophage gene 32 protein (gp32).gp32 was ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.589
更新日期:1996-07-01 00:00:00
abstract::To investigate the contribution of de novo variants to Tourette disorder (TD) probands in China. Whole-exome sequencing (WES) conducted on 15 child-parent trios (45 samples) detected 25 coding de novo variants, including 2 de novo Likely Gene Disrupting (LGD) variants and 6 Missense3 variants. The de novo LGD variants...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4746
更新日期:2020-01-01 00:00:00
abstract::Intestinal phospholipase A/lysophospholipase (IPAL) is an intestine-specific brush-border enzyme expressed during development and along the intestinal crypt-villus axis in a pattern similar to another well characterized brush-border enzyme, sucrase-isomaltase (SI). A tissue-specific DNase I hypersensitive site was ide...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.1419
更新日期:1997-12-01 00:00:00
abstract::The coupling of viral and arthropod host diversity, with evolving methods of virus discovery, has resulted in the identification and classification of a growing number of novel insect-specific viruses (ISVs) that appear to be evolutionarily related to many human pathogens but have either lost or have yet to gain the a...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.4019
更新日期:2017-12-01 00:00:00
abstract::The promoter of the human urokinase plasminogen activator (uPA) gene contains a sequence identical with the retinoblastoma control element (RCE) of the murine c-fos gene, as well as several Sp1 binding sites. In a number of cell lines, the uPA promoter is activated during enforced expression of the retinoblastoma prot...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.1063
更新日期:1994-11-01 00:00:00
abstract::The Wilms' tumor gene 1 (WT1) protein is a transcriptional regulator that is highly expressed in immature hematopoietic progenitor cells and in the majority of patients with acute and chronic myeloid leukemia. However, it is still unclear how WT1 exerts its function(s) in hematopoietic cells. The aim of this work was ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0586
更新日期:2007-08-01 00:00:00
abstract::Genomic instability and epigenetic alterations are distinct hallmarks shared by cancer and aging. Sirtuins (SIRTs) are class III histone deacetylases that regulate gene expression in response to cellular metabolic status. SIRTs can modulate chromatin function through direct deacetylation of histones and by promoting a...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2016.3280
更新日期:2016-10-01 00:00:00
abstract::The major surface glycoprotein (MSG) of Pneumocystis carinii is encoded by a family of related but distinct genes distributed throughout the P. carinii genome. Previous reports of the genomic and mRNA MSG structure suggested that there was a highly conserved 5'-untranslated region and a highly variable translated regi...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.989
更新日期:1996-11-01 00:00:00
abstract::The aims of our research involved to investigate DYS-14 copy number variations in healthy males, to quantify extracellular DNA in maternal circulation in normal versus complicated pregnancies, and to study variations in the DYS-14 copy number in extracellular male fetal DNA. Fifty-five healthy males, 43 uncomplicated ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2009.0855
更新日期:2009-07-01 00:00:00
abstract::Metallothioneins (MT) are expressed during early development in species of several groups. To understand MT function in developmental processes, we studied the MT system of Xenopus laevis, a model vertebrate species in experimental embryology. We first purified and sequenced the liver MT from copper-treated animals. T...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.341
更新日期:1993-05-01 00:00:00
abstract::The distribution and evolutionary pattern of the conserved microsatellite repeat sequences (CA)n, (TGG)6, and (GGAT)4 were studied to determine the divergence time and phylogenetic position of the water buffalo, Bubalus bubalis. The mean allelic frequencies of these repeat loci showed a high level of heterozygosity am...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315231
更新日期:1999-06-01 00:00:00
abstract::The function of retinoic acid-inducible gene-I (RIG-I) in viral replication is well documented, but its function in carcinogenesis and malignancies as well as relationship with microRNAs (miRNAs) remain poorly understood. miR-34a is an antioncogene in multiple tumors. In our study, RIG-I and miR-34a suppressed cell gr...
journal_title:DNA and cell biology
pub_type: 杂志文章,收录出版
doi:10.1089/dna.2015.3130
更新日期:2016-06-01 00:00:00
abstract::To study the elements involved in the pituitary specific transcriptional regulation of the tilapia prolactin I gene (tiPRL I), we have cloned and entirely sequenced a 3.4-kb genomic fragment immediately upstream from the first exon. In footprinting experiments, three tilapia sequences are protected from DNase I digest...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.679
更新日期:1996-08-01 00:00:00
abstract::A subtractive cloning procedure was used to characterize the molecular changes involved in transformation of normal myoblasts to rhabdomyosarcoma (RMS) cells. Here we describe the cloning of DRAL, a novel LIM-domain protein expressed in primary myoblasts but down-regulated in the RMS cell line RD. DRAL is a LIM-only p...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.433
更新日期:1997-04-01 00:00:00
abstract::Streptococcus mutans plays a primary role in the formation of dental caries. Previously, in our laboratory, an S. mutans genomic library was prepared, and the wapA gene was cloned into the shuttle vector, pSA4/4B2. To generate overexpression of wapA and to facilitate efficient purification of the WapA protein for use ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490050085898
更新日期:2000-07-01 00:00:00
abstract::The ras oncogene exists in a variety of human cancers, including carcinomas of bladder, breast, colon, kidney, liver, lung, ovary, pancreas, and stomach. The ras genes acquire transforming activity either by enhanced expression or by a single point mutation. A single base-pair mutation at specific sites within ras gen...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.623
更新日期:1993-09-01 00:00:00
abstract::Transfection of keratinocytes with plasmid DNA leads to the loss of detectable DNA-binding activity of CCAAT displacement protein but not of Yin Yang 1, as monitored by electrophoretic mobility shift assay. This phenomenon was found to be attributable to the presence of plasmid DNA in the nuclear extracts prepared fro...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902753604989
更新日期:2002-02-01 00:00:00
abstract::Disruption of cell-matrix interactions can lead to anoikis-apoptosis due to loss of matrix contacts. We previously showed that Nerve/glial antigen 2 (NG2) is a novel anoikis receptor. Specifically, overexpression of NG2 leads to anoikis propagation, whereas its suppression leads to anoikis attenuation. Interestingly, ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2399
更新日期:2014-12-01 00:00:00
abstract::Xenotransplantation from pigs to human beings is viewed as a potential solution for the acute organ shortage. However, consequent xenorejection induced by Gal alpha 1,3 Gal (a Gal, Gal antigen) prevents xenotransplantation from clinical application. Thus, the most attracting attempt to prevent xenorejection is the eli...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.235
更新日期:2005-04-01 00:00:00
abstract::Colorectal cancer (CRC) patients with KRAS mutation are refractory and usually have poor prognosis. We aimed to identify the hub gene associated with KRAS mutant CRCs. Weighted gene coexpression network analysis (WGCNA) was used to calculate the key module and the hub genes in GSE39582. Combined with the protein-prote...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5574
更新日期:2020-09-01 00:00:00
abstract::Mammalian cumulus-oocyte complexes (COCs) reach full developmental capability during folliculogenesis and oogenesis. It is well recognized that only gametes achieving MII stage after in vivo or in vitro maturation (IVM) are successfully fertilized by a single spermatozoon. Although the process of oocyte nuclear and/or...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3677
更新日期:2017-08-01 00:00:00
abstract::The human ADH1A, ADH1B, and ADH1C genes encode alcohol dehydrogenases (ADHs) that metabolize ethanol. They evolved by recent tandem duplications and have similar proximal cis-acting elements, but differ in tissue-specificity. We hypothesized that distal cis-acting elements confer tissue-specificity. In this article, w...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902320908441
更新日期:2002-11-01 00:00:00