Retinoblastoma control element binding proteins are induced during in vivo terminal differentiation of the liver and bind to the DBP promoter.

abstract：:The potential association of variant surface glycoprotein (VSG) gene expression with clonal expression of virulence in African trypanosomes was addressed. Two populations of clonally related trypanosomes, which differ dramatically in virulence for the infected host, but display the same apparent VSG surface coat pheno...

journal_title：DNA and cell biology

authors： Inverso JA,Uphoff TS,Johnson SC,Paulnock DM,Mansfield JM

更新日期：2010-05-01 00:00:00

abstract：:Electroporation has proven to be a highly effective technique for the in vivo delivery of genes to a number of solid tissues. In most of the reported methods, DNA is injected into the target tissue and electrodes are placed directly on or in the tissue for application of the electric field. While this works well for s...

journal_title：DNA and cell biology

authors： Dean DA

更新日期：2003-12-01 00:00:00

abstract：:The mammalian CYP2E1 gene encodes a cytochrome P450 enzyme that is markedly increased soon after birth. In vitro evidence suggests that the rat CYP2E1 gene is positively regulated by the transcription factor HNF-1 or a protein displaying DNA-binding properties similar to HNF-1. In contrast to newborn wild-type mice an...

journal_title：DNA and cell biology

authors： Gonzalez FJ,Liu SY,Kozak CA,Nebert DW

更新日期：1990-12-01 00:00:00

abstract：:In this study, we isolated two cDNA molecules encoding putative glutamate receptor subunits, fGluR1 alpha and fGluR1 beta, from an Oreochromis sp. brain cDNA library by hybridizing with the glutamate receptor cDNA, fGluR2 beta, of the same fish. The deduced amino acid sequence of the fGluR1 alpha consists of 908 resid...

journal_title：DNA and cell biology

authors： Wu YM,Kung SS,Chen J,Chow WY

更新日期：1996-09-01 00:00:00

abstract：:We have characterized four sequences from a small library containing a subset of the repetitive families of the mouse. Each clone has a repetition frequency of less than 1,100 copies per genome and each clone represents a unique family of middle repetitive DNA. One clone (pMR111) shares homology with mouse intracister...

journal_title：DNA and cell biology

authors： Adiletta DC,Elliott RW,Woodworth ME

更新日期：1993-05-01 00:00:00

abstract：:The gene for the bovine oxytocin receptor has been sequenced using a combination of clones derived from a bovine endometrial cDNA library from estrus and a bovine genomic DNA library, with confirmation of structure using reverse transcription PCR programmed by term myometrial RNA. The receptor belongs to the seven tra...

journal_title：DNA and cell biology

authors： Bathgate R,Rust W,Balvers M,Hartung S,Morley S,Ivell R

更新日期：1995-12-01 00:00:00

abstract：:To study the elements involved in the pituitary specific transcriptional regulation of the tilapia prolactin I gene (tiPRL I), we have cloned and entirely sequenced a 3.4-kb genomic fragment immediately upstream from the first exon. In footprinting experiments, three tilapia sequences are protected from DNase I digest...

journal_title：DNA and cell biology

authors： Poncelet AC,Levavi-Sivan B,Muller M,Yaron Z,Martial JA,Belayew A

更新日期：1996-08-01 00:00:00

abstract：:The human OZF gene encodes a protein consisting essentially of zinc finger motifs of the Krüppel type. Evolutionary studies revealed amplification of the bovine OZF gene in cattle. Domestic cattle includes two major subspecies: taurine (Bos primigenius taurus) and zebu (B. p. indicus). Amplified sequences were found i...

journal_title：DNA and cell biology

authors： Le Chalony C,Apiou F,Pibouin L,Dutrillaux B,Goubin G

更新日期：1996-01-01 00:00:00

abstract：:The human immunodeficiency virus type I (HIV-1) accessory protein Vpr has been associated with the induction of programmed cell death (apoptosis) and cell-cycle arrest. Studies have shown the apoptotic effect of Vpr on primary and established cell lines and on diverse tissues including the central nervous system (CNS)...

journal_title：DNA and cell biology

authors： Cheng X,Mukhtar M,Acheampong EA,Srinivasan A,Rafi M,Pomerantz RJ,Parveen Z

更新日期：2007-02-01 00:00:00

abstract：:To investigate the contribution of de novo variants to Tourette disorder (TD) probands in China. Whole-exome sequencing (WES) conducted on 15 child-parent trios (45 samples) detected 25 coding de novo variants, including 2 de novo Likely Gene Disrupting (LGD) variants and 6 Missense3 variants. The de novo LGD variants...

journal_title：DNA and cell biology

authors： Zhao X,Wang S,Hao J,Zhu P,Zhang X,Wu M

更新日期：2020-01-01 00:00:00

abstract：:The coupling of viral and arthropod host diversity, with evolving methods of virus discovery, has resulted in the identification and classification of a growing number of novel insect-specific viruses (ISVs) that appear to be evolutionarily related to many human pathogens but have either lost or have yet to gain the a...

journal_title：DNA and cell biology

authors： Erasmus JH,Weaver SC

更新日期：2017-12-01 00:00:00

abstract：:Denaturing gradient gel electrophoresis (DGGE) was used to examine error rates and mutations induced by native (wt) and exonuclease-deficient (exo-) Deep Vent DNA polymerases during DNA amplification by polymerase chain reaction (PCR), in the presence or absence of the T4 bacteriophage gene 32 protein (gp32).gp32 was ...

journal_title：DNA and cell biology

authors： Huang H,Keohavong P

更新日期：1996-07-01 00:00:00

abstract：:A yeast two-hybrid system was used to identify mutants of Max that exhibit an increased affinity for Myc. Truncated forms of the Max helix-loop-helix/leucine zipper motif (HLH/Zip) were first expressed in a two- hybrid system in which the bait protein was the HLH/Zip motif of Myc. Deletion of amino acids both amino-te...

journal_title：DNA and cell biology

authors： Wang H,Van Den Bergh F,Spencer E,Wilcox K,Herman T

更新日期：1997-11-01 00:00:00

abstract：:Exposure to microgravity is supposed to affect almost all biological systems, and we speculated that microgravity is potentially involved in autophagy regulation. A clinostat was used to simulate microgravity, and HEK293 cells that stably express GFP-LC3 were used for sensitive monitoring of autophagy induction. The c...

journal_title：DNA and cell biology

authors： Ryu HW,Choi SH,Namkoong S,Jang IS,Seo DH,Choi I,Kim HS,Park J

更新日期：2014-03-01 00:00:00

abstract：:Nitric oxide synthase (NOS) is the enzyme responsible for the conversion of L-arginine to L-citrulline and nitric oxide. Dimerization of the enzyme is an absolute requirement for catalytic activity. Each NOS monomer contains an N-terminal heme-binding domain and a C-terminal reductase domain. It is unclear how the red...

journal_title：DNA and cell biology

authors： Hallmark OG,Phung YT,Black SM

更新日期：1999-05-01 00:00:00

abstract：:KCNQ1 overlapping transcript 1 (KCNQ1OT1), a long noncoding RNA responsible for silencing a cluster of genes in cis, has been shown to be involved in multiple cancers. However, much remains unclear of how KCNQ1OT1 contributes to carcinogenesis. By thoroughly analyzing 510 hepatocellular carcinoma (HCC) cases and 1014 ...

journal_title：DNA and cell biology

authors： Wan J,Huang M,Zhao H,Wang C,Zhao X,Jiang X,Bian S,He Y,Gao Y

更新日期：2013-11-01 00:00:00

abstract：:NANOG is a critical homeodomain transcription factor responsible for maintaining embryonic stem cell (ESC) self-renewal and pluripotency. In the present study, we isolated, sequenced, and characterized the NANOG gene in buffalo ESC-like cells. Here, we demonstrated that NANOG mRNA is expressed as multiple isoforms and...

journal_title：DNA and cell biology

authors： Singh N,Sharma R,George A,Singla SK,Palta P,Manik R,Chauhan MS,Singh D

更新日期：2012-05-01 00:00:00

abstract：:A decrease in gap junctional, intercellular communication (GJIC) has been associated with cells neoplastically transformed by a variety of factors. To investigate the role of the Ras oncogene product in gap junction function, a panel of murine C3H10T1/2 (10T1/2) fibroblasts was constructed in which the levels of ras g...

journal_title：DNA and cell biology

authors： Brownell HL,Narsimhan RP,Corbley MJ,Mann VM,Whitfield JF,Raptis L

更新日期：1996-06-01 00:00:00

abstract：:Endocytosis of the five rat somatostatin receptor subtypes (SSTR1-5) was investigated in transfected HEK cells by biochemical ligand binding assays and confocal microscopic analysis. Phenylarsine oxide-sensitive internalization of SSTR1-3 is dependent on SST-14 or SST-28, whereas only the octacosapeptide triggers this...

journal_title：DNA and cell biology

authors： Roth A,Kreienkamp HJ,Nehring RB,Roosterman D,Meyerhof W,Richter D

更新日期：1997-01-01 00:00:00

abstract：:Methionine is the limiting amino acid for milk protein synthesis in dairy cows. The effect of methionine availability on milk protein synthesis is dependent on its active transport into cells through amino acid transporters. L-type amino acid transporter 1 (LAT1), which induces the transport of neutral amino acids, is...

journal_title：DNA and cell biology

authors： Duan X,Lin Y,Lv H,Yang Y,Jiao H,Hou X

更新日期：2017-12-01 00:00:00

abstract：:Oxysterols are oxygenated derivatives of cholesterol. They have been shown to influence a variety of biological functions including sterol metabolism, lipid trafficking, and apoptosis. Recently, 12 human OSBP-related genes have been identified. In this study, we have identified a family of 12 oxysterol-binding protein...

journal_title：DNA and cell biology

authors： Anniss AM,Apostolopoulos J,Dworkin S,Purton LE,Sparrow RL

更新日期：2002-08-01 00:00:00

abstract：:Recently, we demonstrated that the DAN gene product contains a growth- and/or a tumor-suppressive activity in vitro. In the present work, using a yeast two-hybrid system, we searched for cellular proteins that can associate with the DAN gene product. A cDNA clone, termed DA41, was initially isolated from an adult rat ...

journal_title：DNA and cell biology

authors： Ozaki T,Hishiki T,Toyama Y,Yuasa S,Nakagawara A,Sakiyama S

更新日期：1997-08-01 00:00:00

abstract：:Transgenic mice expressing interleukin-12 (IL-12) under the glial fibrillary acidic protein (GFAP) promoter were evaluated for their sensitivity to herpes simplex virus type 1 (HSV-1) infection of the cornea. There was a modest but significant decrease in the infiltration of mononuclear cells in the cornea of the GFAP...

journal_title：DNA and cell biology

authors： Carr DJ,Ash J,Al-Khatib K,Campbell IL

更新日期：2002-05-01 00:00:00

abstract：:Phytoplasmas are nonculturable cell wall-less, obligate intracellular pathogens of plants and insect vectors. In their descent from walled bacterial ancestors, phytoplasmas underwent massive genome reduction, resulting in some of the smallest cellular genomes known in nonsymbiotic bacteria. While requirements for in v...

journal_title：DNA and cell biology

authors： Davis RE,Jomantiene R,Zhao Y

更新日期：2005-12-01 00:00:00

abstract：:Antibody-targeting therapy has drawn great interests to the hematologists and oncologists. 3A4, a novel antibody recognizing human CD45RA antigen, is a new target molecule for leukemias and holds a therapeutic potential for myeloid lineage leukemias. However, murine antibodies cannot be safely used in patients because...

journal_title：DNA and cell biology

authors： Li S,Shen D,Guo X,Liao C,Tang Y

更新日期：2018-09-01 00:00:00

abstract：:The aim of this study was to determine the role of polymorphic variants of apolipoprotein E (APOE) and peroxisome proliferator-activated receptor delta (PPARD) genes in the development of coronary heart disease (CHD), and the PPARD and APOE gene-gene interaction in a Turkish population. This study was carried out usin...

journal_title：DNA and cell biology

authors： Yılmaz-Aydogan H,Kucukhuseyin O,Kurnaz O,Akadam-Teker B,Kurt O,Tekeli A,Ozturk O,Isbir T

更新日期：2012-05-01 00:00:00

abstract：:Culture of CHO.K1, a Chinese hamster ovary cell line, requires no particular care about the glucocorticoid level in media. Cell growth of CHO.K1 is little affected by dexamethasone at concentrations up to 3 microM. A clone of CHO.K1 stably transfected with an expression vector displayed a favored growth in dexamethaso...

journal_title：DNA and cell biology

authors： Chen GS,Lee CH,Lee YC,Chi CW,Liu YC

更新日期：1996-02-01 00:00:00

abstract：:We have analyzed the interleukin-1 beta (IL-1 beta) promoter region near the cap site. Specific DNA sequences required for lipopolysaccharide (LPS) induction within this region were identified using transfection of reporter plasmids that contained portions of the proximal IL-1 beta 5'-flanking sequence. An LPS-respons...

journal_title：DNA and cell biology

authors： Godambe SA,Chaplin DD,Takova T,Bellone CJ

更新日期：1994-06-01 00:00:00

abstract：:Gene therapy depends on safe and efficient gene delivery. The skin is an attractive target for gene delivery because of its accessibility. Recently, in vivo electroporation has been shown to enhance expression after injection of plasmid DNA. In this study, we examined the use of electroporation to deliver plasmid DNA ...

journal_title：DNA and cell biology

authors： Heller R,Schultz J,Lucas ML,Jaroszeski MJ,Heller LC,Gilbert RA,Moelling K,Nicolau C

更新日期：2001-01-01 00:00:00

abstract：:A constituent of green tea, (-)-epigallocatechin-3-gallate (EGCG) has been known to possess antiproliferative properties. In this study, we investigated the anticancer effects of EGCG in human papillomavirus (HPV)-16 associated cervical cancer cell line, CaSki cells. The growth inhibitory mechanism(s) and regulation o...

journal_title：DNA and cell biology

authors： Ahn WS,Huh SW,Bae SM,Lee IP,Lee JM,Namkoong SE,Kim CK,Sin JI

更新日期：2003-03-01 00:00:00