A major constituent of green tea, EGCG, inhibits the growth of a human cervical cancer cell line, CaSki cells, through apoptosis, G(1) arrest, and regulation of gene expression.


:A constituent of green tea, (-)-epigallocatechin-3-gallate (EGCG) has been known to possess antiproliferative properties. In this study, we investigated the anticancer effects of EGCG in human papillomavirus (HPV)-16 associated cervical cancer cell line, CaSki cells. The growth inhibitory mechanism(s) and regulation of gene expression by EGCG were also evaluated. EGCG showed growth inhibitory effects in CaSki cells in a dose-dependent fashion, with an inhibitory dose (ID)(50) of approximately 35 microM. When CaSki cells were further tested for EGCG-induced apoptosis, apoptotic cells were significantly observed after 24 h at 100 microM EGCG. In contrast, an insignificant induction of apoptotic cells was observed at 35 microM EGCG. However, cell cycles at the G1 phase were arrested at 35 microM EGCG, suggesting that cell cycle arrests might precede apoptosis. When CaSki cells were tested for their gene expression using 384 cDNA microarray, an alteration in the gene expression was observed by EGCG treatment. EGCG downregulated the expression of 16 genes over time more than twofold. In contrast, EGCG upregulated the expression of four genes more than twofold, suggesting a possible gene regulatory role of EGCG. This data supports that EGCG can inhibit cervical cancer cell growth through induction of apoptosis and cell cycle arrest as well as regulation of gene expression in vitro. Furthermore, in vivo antitumor effects of EGCG were also observed. Thus, EGCG likely provides an additional option for a new and potential drug approach for cervical cancer patients.


DNA Cell Biol


DNA and cell biology


Ahn WS,Huh SW,Bae SM,Lee IP,Lee JM,Namkoong SE,Kim CK,Sin JI





Has Abstract


2003-03-01 00:00:00












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    abstract::Chlamydial infection of the lower genital tract usually spreads to the upper genital tract and is then responsible for more serious consequences, such as infertility, ectopic pregnancy, pelvic pain, and pelvic inflammatory disease. Genital infection with Chlamydia trachomatis and the resulting cytokine response largel...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Srivastava P,Bhengraj AR,Jha HC,Vardhan H,Jha R,Singh LC,Salhan S,Mittal A

    更新日期:2012-03-01 00:00:00

  • Expression of human bone morphogenetic proteins-2 or -4 in murine mesenchymal progenitor C3H10T1/2 cells induces differentiation into distinct mesenchymal cell lineages.

    abstract::The cDNAs encoding the human bone morphogenetic proteins BMP-2 and BMP-4 in an eukaryotic expression vector were permanently transferred into the murine mesenchymal progenitor cell line C3H10T1/2. Originally, these cells are known to differentiate into myotubes, adipocytes, and chondrocytes upon the addition of azacyt...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Ahrens M,Ankenbauer T,Schröder D,Hollnagel A,Mayer H,Gross G

    更新日期:1993-12-01 00:00:00

  • Three human homologs of a murine gene encoding an inhibitor of stem cell proliferation.

    abstract::The G0S19 genes are members of the "small inducible" family of genes, which have similar exon-intron organizations and encode secreted proteins with similar dispositions of cysteine and proline residues. G0S19-1 mRNA is increased shortly after the addition of lectin or cycloheximide to cultured human blood mononuclear...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Blum S,Forsdyke RE,Forsdyke DR

    更新日期:1990-10-01 00:00:00

  • Unlike Butylcycloheptylprodigiosin, Isolated Undecylprodigiosin from Streptomyces parvulus Is Not a MDR1 and BCRP Substrate in Multidrug-Resistant Cancers.

    abstract::The search for new chemotherapeutics unaffected by efflux pumps would significantly increase life expectancy in patients with malignant cancers. In this study, butylcycloheptylprodigiosin and undecylprodigiosin were HPLC-purified and verified, using nuclear magnetic resonance spectroscopy. Cell cytotoxicity and transp...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Mirzaei SA,Safari Kavishahi M,Keshavarz Z,Elahian F

    更新日期:2018-06-01 00:00:00

  • "Bone Development" Is an Ontology Group Upregulated in Porcine Oocytes Before In Vitro Maturation: A Microarray Approach.

    abstract::Mammalian cumulus-oocyte complexes (COCs) reach full developmental capability during folliculogenesis and oogenesis. It is well recognized that only gametes achieving MII stage after in vivo or in vitro maturation (IVM) are successfully fertilized by a single spermatozoon. Although the process of oocyte nuclear and/or...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Budna J,Bryja A,Celichowski P,Kranc W,Ciesiółka S,Borys S,Rybska M,Kolecka-Bednarczyk A,Jeseta M,Bukowska D,Antosik P,Brüssow KP,Bruska M,Nowicki M,Zabel M,Kempisty B

    更新日期:2017-08-01 00:00:00

  • Identification of a Novel Six-Long Noncoding RNA Signature for Molecular Diagnosis of Dilated Cardiomyopathy.

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    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Zhang H,Chen X,Zhang D,Liu L,Song J,Xu Y,Tian J

    更新日期:2020-11-03 00:00:00

  • Endocytosis of the rat somatostatin receptors: subtype discrimination, ligand specificity, and delineation of carboxy-terminal positive and negative sequence motifs.

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    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Roth A,Kreienkamp HJ,Nehring RB,Roosterman D,Meyerhof W,Richter D

    更新日期:1997-01-01 00:00:00

  • Transcriptional activation by the zinc-finger homeodomain protein delta EF1 in estrogen signaling cascades.

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    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Dillner NB,Sanders MM

    更新日期:2004-01-01 00:00:00

  • MMP7 -181G allele is a low-penetrant risk factor for cancer development in East Asians.

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    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Yuan-Yuan M,Li-Feng Z,Li-Jie Z

    更新日期:2012-05-01 00:00:00

  • Downstream sequence adjacent to AUG affects translation of chloramphenicol acetyl transferase in eukaryotic cells.

    abstract::The CAT gene is widely used as a reporter in eukaryotic systems because of the efficient translation of its mRNA. We report here that a sequence occurring in the CAT mRNA at +15 nucleotides from CAT AUG is essential for translation. This sequence includes a stem-loop structure, which, however, exhibits a calculated st...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Ciullo M,Del Pozzo G,Autiero M,Guardiola J

    更新日期:2000-01-01 00:00:00

  • Abnormalities in the T cell receptor Vδ repertoire and Foxp3 expression in refractory anemia with ringed sideroblasts.

    abstract::Immune dysregulation has been implicated in myelodysplastic syndrome (MDS) pathogenesis. Refractory anemia with ringed sideroblasts (RARS) is a low-risk subtype of MDS. Our previous study has reported an abnormal γδ T cell receptor (TCR) repertoire in patients with intermediate or high-risk MDS. To characterize the st...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Geng S,Weng J,Chen S,Li Y,Wu P,Huang X,Lai P,Du X

    更新日期:2015-09-01 00:00:00

  • Expression and Clinical Significance of CMTM6 in Nonsmall Cell Lung Cancer.

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    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Hou X,He S,Zhang D,Yang C,Shi Y,Zhang K

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  • Cloning and sequencing of the rat preproepidermal growth factor cDNA: comparison with mouse and human sequences.

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    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Saggi SJ,Safirstein R,Price PM

    更新日期:1992-07-01 00:00:00

  • Association of Long Noncoding RNAs Polymorphisms with the Risk of Esophagogastric Junction Adenocarcinoma: A Three-Center Study of 1063 Cases and 1677 Controls.

    abstract::Increasing evidence suggested that long noncoding RNAs (lncRNAs) variants may be involved in the progression of various cancers. However, the association of the lncRNAs polymorphisms with the risk for esophagogastric junction adenocarcinoma (EGJA) is still unknown. In this case-control study, we selected two cancer-re...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Cao R,Chen Y,Wang J,Chen M,Chen S,Tang W

    更新日期:2020-05-01 00:00:00

  • Complete structure and expression of the rat alpha B-crystallin gene.

    abstract::alpha B-Crystallin, a member of the small heat shock family of proteins, is synthesized as a component of various developmental programs, in response to stress and in a number of pathological states. We have determined the complete structure of the alpha B-crystallin gene (6,806 bp encompassing 2,299 bp upstream from ...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Srinivasan AN,Bhat SP

    更新日期:1994-06-01 00:00:00

  • Point mutation of the ras oncogene in human ovarian cancer.

    abstract::The ras oncogene exists in a variety of human cancers, including carcinomas of bladder, breast, colon, kidney, liver, lung, ovary, pancreas, and stomach. The ras genes acquire transforming activity either by enhanced expression or by a single point mutation. A single base-pair mutation at specific sites within ras gen...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Chien CH,Chow SN

    更新日期:1993-09-01 00:00:00

  • Functional variants in NOS1 and NOS2A are not associated with progressive hearing loss in Ménière's disease in a European Caucasian population.

    abstract::Hearing loss in Ménière's disease (MD) is associated with loss of spiral ganglion neurons and hair cells. In a guinea pig model of endolymphatic hydrops, nitric oxide synthases (NOS) and oxidative stress mediate loss of spiral ganglion neurons. To test the hypothesis that functional variants of NOS1 and NOS2A are asso...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Gazquez I,Lopez-Escamez JA,Moreno A,Campbell CA,Meyer NC,Carey JP,Minor LB,Gantz BJ,Hansen MR,Della Santina CC,Aran I,Soto-Varela A,Santos S,Batuecas A,Perez-Garrigues H,Lopez-Nevot A,Smith RJ,Lopez-Nevot MA

    更新日期:2011-09-01 00:00:00

  • A retroviral repetitive element confers tissue-specificity to the human alcohol dehydrogenase 1C (ADH1C) gene.

    abstract::The human ADH1A, ADH1B, and ADH1C genes encode alcohol dehydrogenases (ADHs) that metabolize ethanol. They evolved by recent tandem duplications and have similar proximal cis-acting elements, but differ in tissue-specificity. We hypothesized that distal cis-acting elements confer tissue-specificity. In this article, w...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Chen HJ,Carr K,Jerome RE,Edenberg HJ

    更新日期:2002-11-01 00:00:00

  • Synchronous block in DNA synthesis initiation with change in chromatin topology mediated by VP16.

    abstract::The impact of chromatin topology on the DNA synthetic process was studied in the human squamous-cell carcinoma cell line SQ-20B. A 1-h exposure < or = 10 microM VP16 produced an increase in DNA supercoil tension, measured by recording laser light scatter from salt-extracted nuclei. This change was precisely paralleled...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Narayana A,Khodarev N,Walter S,Vaughan AT

    更新日期:1998-07-01 00:00:00

  • Use of the polymerase chain reaction for the detection of alternatively spliced mRNAs of plasma membrane calcium pump.

    abstract::Polymerase chain reaction [PCR, reverse transcriptase-PCR (RT-PCR)] has been used to amplify the mRNA subspecies of the plasma membrane calcium pump isoform 1 (PMCA1) in total RNA extracted from hamster tissues. Two primers were synthesized that encompass the site at which a 154-bp exon is included totally (PMCA1a), p...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Sarkar FH,Ball DE,Tsang W,Li YW,Kuo TH

    更新日期:1993-06-01 00:00:00

  • Isolation and characterization of a cDNA encoding a chicken beta thyroid hormone receptor.

    abstract::We have isolated and characterized a cDNA encoding a chicken beta homolog of c-erbA, or thyroid hormone receptor (TR). Chicken liver cDNA libraries were screened with a rat TR beta-1 cDNA probe, and several cDNA inserts were isolated and characterized. The sequence of one cDNA predicts a 369-amino-acid open reading fr...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Showers MO,Darling DS,Kieffer GD,Chin WW

    更新日期:1991-04-01 00:00:00

  • p53 gene expression and 2-methoxyestradiol treatment differentially induce nuclear factor kappa B activation in human lung cancer cells with different p53 phenotypes.

    abstract::The p53 tumor suppressor gene is frequently mutated in multiple human cancers, leading to loss of wild-type p53 (wt-p53)-dependent functions and tumorigenesis. p53 gene therapy is used to induce apoptosis in human cancer cells and tumors. Activation of nuclear factor kappa B (NF-kappaB) causes resistance to both chemo...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Rath PC,Mukhopadhyay T

    更新日期:2009-12-01 00:00:00

  • Genomic organization of the genes for human and mouse CC chemokine LEC.

    abstract::Liver-expressed chemokine (LEC) is a CC chemokine that is selectively expressed in the liver. We report here the structures of the human and mouse genes for LEC. The human LEC gene (SCYA16) was isolated from a bacterial artificial chromosome (BAC) clone that also contained CC chemokine genes for MPIF-1/Ckbeta8, HCC-2/...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Fukuda S,Hanano Y,Iio M,Miura R,Yoshie O,Nomiyama H

    更新日期:1999-04-01 00:00:00

  • Structure of the chum salmon insulin-like growth factor I gene.

    abstract::Insulin-like growth factor I (IGF-I) plays a major role in development and metabolism. Currently, the cDNA-derived primary structure of IGF-I is known for some mammals and for chicken, frog, and salmon. Additionally, the organization of the human, rat, and chicken IGF-I genes has been established. The investigation of...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Kavsan VM,Koval AP,Grebenjuk VA,Chan SJ,Steiner DF,Roberts CT Jr,LeRoith D

    更新日期:1993-10-01 00:00:00

  • Molecular genetic relationship of the 3' untranslated region among Thai dengue-3 virus, Bangkok isolates, during 1973-2000.

    abstract::Dengue virus serotype 3 (DENV-3) was associated with severe dengue epidemics in Thailand during 1973-1999. We studied Thai DENV-3 viruses isolated from hospitalized children in Bangkok with differing disease severity during that period. Viruses were sequenced at their 5' and 3' untranslated regions (UTRs), which are r...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Pankhong P,Weiner DB,Ramanathan MP,Nisalak A,Kalayanarooj S,Nimmannitya S,Attatippaholkun W

    更新日期:2009-10-01 00:00:00

  • ALDH2 rs671 Polymorphism and coronary heart disease risk among Asian populations: a meta-analysis and meta-regression.

    abstract::Mitochondrial aldehyde dehydrogenase-2 (ALDH2) is the principal enzyme involved in alcohol metabolism in humans. Emerging evidence has shown that the common rs671 G>A (Glu504Lys) polymorphism in the ALDH2 gene might play a critical role in increasing the susceptibility to coronary heart disease (CHD), including myocar...

    journal_title:DNA and cell biology

    pub_type: 杂志文章,meta分析


    authors: Wang Q,Zhou S,Wang L,Lei M,Wang Y,Miao C,Jin Y

    更新日期:2013-07-01 00:00:00

  • Heme oxygenase and the kidney.

    abstract::Heme plays a significant pathogenic role in several diseases involving the kidney. The cellular content of heme, derived either from the delivery of filtered heme proteins such as hemoglobin and myoglobin, or from the breakdown of ubiquitous intracellular heme proteins, is regulated via the heme oxygenase enzyme syste...

    journal_title:DNA and cell biology

    pub_type: 杂志文章,评审


    authors: Hill-Kapturczak N,Chang SH,Agarwal A

    更新日期:2002-04-01 00:00:00

  • CYP24A1 Inhibition Facilitates the Antiproliferative Effect of 1,25(OH)2D3 Through Downregulation of the WNT/β-Catenin Pathway and Methylation-Mediated Regulation of CYP24A1 in Colorectal Cancer Cells.

    abstract::CYP24A1 is overexpressed in colorectal cancer, and the reason for the dysregulation of CYP24A1 in colorectal cancer is still unknown. In the present study, experiments were designed to test whether CYP24A1 inhibition facilitated the antiproliferative effect of 1,25(OH)2D3. In addition, the role of methylation in the r...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Sun H,Jiang C,Cong L,Wu N,Wang X,Hao M,Liu T,Wang L,Liu Y,Cong X

    更新日期:2018-09-01 00:00:00

  • HMGA1 Mediated High-Glucose-Induced Vascular Smooth Muscle Cell Proliferation in Diabetes Mellitus: Association Between PI3K/Akt Signaling and HMGA1 Expression.

    abstract::High-mobility group protein A1 (HMGA1), an architectural transcription factor, was found to regulate multiple gene expression in mammals. Recent studies firmly indicate an association between HMGA1 and type 2 diabetes. However, the presence and function of HMGA1 in diabetic vasculopathy has not been substantiated. in ...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Zhang Q,Chen L,Zhao Z,Wu Y,Zhong J,Wen G,Cao R,Zu X,Liu J

    更新日期:2018-04-01 00:00:00

  • Characterization of genetic variation in the Forkhead box class O3 gene (FOXO3) in sheep.

    abstract::FOXO3 is a member of the Forkhead box class O (FOXO) transcription factor family that modulates the transcription of target genes in a variety of cellular functions in lower animals to mammals. It has been implicated in the attenuation of insulin-signaling pathways, including having a potential role in lifespan contro...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Byun SO,Zhou H,Hickford JG

    更新日期:2011-07-01 00:00:00