Abstract:
:MicroRNAs (miRNAs) are an important class of small, noncoding, regulatory RNAs found to be involved in regulating a wide variety of important cellular processes by the sequence-specific inhibition of gene expression. Viruses have evolved a number of mechanisms to take advantage of the regulatory potential of this highly conserved, ubiquitous pathway known as RNA interference (RNAi). This review will focus on the recent efforts to understand the complex relationship between vertebrate viruses and the RNAi pathway, as well as the role of silencing pathways in the inhibition of pathogenic genetic elements, including transposons and retrotransposons.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Dykxhoorn DMdoi
10.1089/dna.2006.0559subject
Has Abstractpub_date
2007-04-01 00:00:00pages
239-49issue
4eissn
1044-5498issn
1557-7430journal_volume
26pub_type
杂志文章,评审abstract::Insulin-like growth factor I (IGF-I) plays a major role in development and metabolism. Currently, the cDNA-derived primary structure of IGF-I is known for some mammals and for chicken, frog, and salmon. Additionally, the organization of the human, rat, and chicken IGF-I genes has been established. The investigation of...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.729
更新日期:1993-10-01 00:00:00
abstract::The activation of H-plk (Human-proviral linked Krüppel), a human Krüppel-related zinc finger gene in organs such as placenta, adrenal cortex, and testis, is probably due to insertion of an endogenous retrovirus, ERV3, upstream of the gene. Several differently spliced transcripts originate from this locus, e.g., a tran...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.27
更新日期:1998-01-01 00:00:00
abstract::Cardiomyocytes are notoriously difficult to transfect using standard techniques unless viral vectors such as recombinant adenoviruses are used. Generation of recombinant adenoviruses is, however, a complex and time-consuming procedure and not possible for every DNA construct. We therefore optimized DNA/polylysine/aden...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.381
更新日期:2005-06-01 00:00:00
abstract::Cytochrome P450 15 beta is a female-specific gene product that catalyzes the hydroxylation of steroid sulfates at the 15 beta position. Isolation and analysis of the gene for rat P450 15 beta reveals nine coding exons and encompasses more than 35 kb of chromosomal DNA. The intron-exon junctions are at similar position...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.49
更新日期:1990-01-01 00:00:00
abstract::The cDNAs encoding the human bone morphogenetic proteins BMP-2 and BMP-4 in an eukaryotic expression vector were permanently transferred into the murine mesenchymal progenitor cell line C3H10T1/2. Originally, these cells are known to differentiate into myotubes, adipocytes, and chondrocytes upon the addition of azacyt...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.871
更新日期:1993-12-01 00:00:00
abstract::Based on the concept of sequence conservation around the active sites of serine proteinases, polymerase chain reaction applied to mRNA amplification allowed us to obtain a 260-bp probe which was used to screen a mouse pituitary cDNA library. The primers used derived from the cDNA sequence of active sites Ser* and Asn*...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.415
更新日期:1990-07-01 00:00:00
abstract:PURPOSE:This case-control study was conducted in order to evaluate the potential role of polymorphic genes encoding enzymes involved in estrogen biosynthesis (CYP19A1) and metabolism (GSTM1, GSTT1, and GSTP1), and their action in modulating individual susceptibility to breast cancer. METHODS:Genomic DNA was extracted ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1538
更新日期:2012-06-01 00:00:00
abstract::We have characterized four sequences from a small library containing a subset of the repetitive families of the mouse. Each clone has a repetition frequency of less than 1,100 copies per genome and each clone represents a unique family of middle repetitive DNA. One clone (pMR111) shares homology with mouse intracister...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.319
更新日期:1993-05-01 00:00:00
abstract::Nd1-S is the nuclear-localizing short variant form of Nd1 (Ivns1abp) encoding a Kelch family transcription factor. While the function of Nd1 has been investigated in the context of metastasis and doxorubicin-induced cardiotoxicity, little is known about its role in hematopoiesis. In this study, we investigated the fun...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4206
更新日期:2018-10-01 00:00:00
abstract::Our aim was to evaluate in a cohort of 125 Italian patients with type 2 diabetes (T2D), who underwent neurological evaluation, the possible differences in the number of mitochondrial DNA copies (mtDNA) comparing positive and negative patients for cardiovascular autonomic neuropathy (CAN) or diabetic peripheral neuropa...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5326
更新日期:2020-08-01 00:00:00
abstract::A nomenclature system for the UDP glucuronosyltransferase superfamily is proposed, based on divergent evolution of the genes. A total of 26 distinct cDNAs in five mammalian species have been sequenced to date. Comparison of the deduced amino acid sequences leads to the definition of two families and a total of three s...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.1991.10.487
更新日期:1991-09-01 00:00:00
abstract::Tumor cells express lower levels of manganese superoxide dismutase (MnSOD) than their normal counterparts. Enforced expression of MnSOD reverses the malignant phenotype of many transformed cells, suggesting that SOD2 is a tumor suppressor. The SOD2 gene contains a large CpG island spanning > 3.5 kb that starts near th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315051
更新日期:1999-08-01 00:00:00
abstract::Increasing evidence suggested that long noncoding RNAs (lncRNAs) variants may be involved in the progression of various cancers. However, the association of the lncRNAs polymorphisms with the risk for esophagogastric junction adenocarcinoma (EGJA) is still unknown. In this case-control study, we selected two cancer-re...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5368
更新日期:2020-05-01 00:00:00
abstract::Increasing evidence has announced the emerging roles of long noncoding RNAs (lncRNAs) in modulating bone homeostasis due to their potential regulating effects on bone-related cells' proliferation, migration, differentiation and apoptosis. Thus, lncRNAs have been considered as a promising gene tool to facilitate the bo...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2020.5391
更新日期:2020-06-01 00:00:00
abstract::CYP24A1 is overexpressed in colorectal cancer, and the reason for the dysregulation of CYP24A1 in colorectal cancer is still unknown. In the present study, experiments were designed to test whether CYP24A1 inhibition facilitated the antiproliferative effect of 1,25(OH)2D3. In addition, the role of methylation in the r...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.4058
更新日期:2018-09-01 00:00:00
abstract::FOXO3 is a member of the Forkhead box class O (FOXO) transcription factor family that modulates the transcription of target genes in a variety of cellular functions in lower animals to mammals. It has been implicated in the attenuation of insulin-signaling pathways, including having a potential role in lifespan contro...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2010.1193
更新日期:2011-07-01 00:00:00
abstract::Liver-expressed chemokine (LEC) is a CC chemokine that is selectively expressed in the liver. We report here the structures of the human and mouse genes for LEC. The human LEC gene (SCYA16) was isolated from a bacterial artificial chromosome (BAC) clone that also contained CC chemokine genes for MPIF-1/Ckbeta8, HCC-2/...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315330
更新日期:1999-04-01 00:00:00
abstract::Viral factor has been implicated in the etiopathogenesis of vitiligo. To elucidate the effects of viral double-stranded RNA (dsRNA) on melanocytes and to explore the underlying mechanisms, primary cultured normal human melanocytes were treated with synthetic viral dsRNA analog poly(I:C). The results demonstrated that ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2711
更新日期:2015-06-01 00:00:00
abstract::The p53 tumor suppressor gene is frequently mutated in multiple human cancers, leading to loss of wild-type p53 (wt-p53)-dependent functions and tumorigenesis. p53 gene therapy is used to induce apoptosis in human cancer cells and tumors. Activation of nuclear factor kappa B (NF-kappaB) causes resistance to both chemo...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2008.0839
更新日期:2009-12-01 00:00:00
abstract::The function of retinoic acid-inducible gene-I (RIG-I) in viral replication is well documented, but its function in carcinogenesis and malignancies as well as relationship with microRNAs (miRNAs) remain poorly understood. miR-34a is an antioncogene in multiple tumors. In our study, RIG-I and miR-34a suppressed cell gr...
journal_title:DNA and cell biology
pub_type: 杂志文章,收录出版
doi:10.1089/dna.2015.3130
更新日期:2016-06-01 00:00:00
abstract::We have analyzed the interleukin-1 beta (IL-1 beta) promoter region near the cap site. Specific DNA sequences required for lipopolysaccharide (LPS) induction within this region were identified using transfection of reporter plasmids that contained portions of the proximal IL-1 beta 5'-flanking sequence. An LPS-respons...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.561
更新日期:1994-06-01 00:00:00
abstract::The human OZF gene encodes a protein consisting essentially of zinc finger motifs of the Krüppel type. Evolutionary studies revealed amplification of the bovine OZF gene in cattle. Domestic cattle includes two major subspecies: taurine (Bos primigenius taurus) and zebu (B. p. indicus). Amplified sequences were found i...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.83
更新日期:1996-01-01 00:00:00
abstract::The Wilms' tumor gene 1 (WT1) protein is a transcriptional regulator that is highly expressed in immature hematopoietic progenitor cells and in the majority of patients with acute and chronic myeloid leukemia. However, it is still unclear how WT1 exerts its function(s) in hematopoietic cells. The aim of this work was ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0586
更新日期:2007-08-01 00:00:00
abstract::DNA methylation of CYP17 (steroid 17 alpha-hydroxylase) was studied in bovine adrenocortical cells, which lose the capacity to express this tissue-specific gene in culture by phenotypic switching. Restriction enzyme digestions, and sequencing of a lambda clone of a second CYP17 gene (CYP17A2), showed that there are at...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.385
更新日期:1992-06-01 00:00:00
abstract::Streptococcus mutans plays a primary role in the formation of dental caries. Previously, in our laboratory, an S. mutans genomic library was prepared, and the wapA gene was cloned into the shuttle vector, pSA4/4B2. To generate overexpression of wapA and to facilitate efficient purification of the WapA protein for use ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490050085898
更新日期:2000-07-01 00:00:00
abstract::Porifera (sponges) constitute the lowest metazoan phylum. Experiments examined whether sponges can recognize self/nonself molecules. Cells from the marine sponge Suberites domuncula were incubated with membranes from either S. domuncula or another marine sponge, Geodia cydonium, as well as with recombinant alpha-integ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999314746
更新日期:1999-12-01 00:00:00
abstract::In a systematic approach to identify interferon-gamma (IFN-gamma)-regulated host effector molecules, we found several members of the 65 kDa guanylate-binding proteins (GBPs) highly upregulated. During extensive characterization of these guanosine triphosphatases (GTPases), we identified discrepancies between the clone...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0637
更新日期:2007-12-01 00:00:00
abstract::Myocardial ischemic disease affects the prognosis in perioperative patients. Diabetes can aggravate myocardial injury. The purpose of this research is to investigate the effect of ferroptosis in the process of diabetes mellitus (DM) myocardial ischemia/reperfusion (I/R) injury (IRI). Endoplasmic reticulum stress (ERS)...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5097
更新日期:2020-02-01 00:00:00
abstract::Accumulating evidence has suggested that microRNAs play important roles in the development of hepatocellular carcinoma (HCC) and are involved in drug resistance. miR-21-5p was overexpressed in a variety of cancers and promoted the tumorigenesis; however, the function of miR-21-5p in HCC still remains unknown. In this ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4529
更新日期:2019-08-01 00:00:00
abstract::The computer program, SIGSEQ2, was used to select heterologous signal peptides from a catalog of published sequences to express the echistatin gene in insect (Sf9) cells. S-values for each amino acid were determined to select empirically the site of cleavage between the signal peptide and mature echistatin. Five gene ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.453
更新日期:1990-07-01 00:00:00