Abstract：
:Escherichia coli, Salmonella enterica, Klebsiella pneumoniaeand Klebsiella oxytocawere found to contain two D-tagatose 1,6-bisphosphate (TagBP)-specific aldolases involved in catabolism of galactitol (genes gatY gatZ) and of N-acetyl-galactosamine and D-galactosamine (genes kbaY kbaZ,also called agaY agaZ). The two aldolases were closely related (> or = 53.8% identical amino acids) and could substitute for each other in vivo. The catalytic subunits GatY or KbaY alone were sufficient to show aldolase activity. Although substantially shorter than other aldolases (285 amino acids, instead of 358 and 349 amino acids), these subunits contained most or all of the residues that have been identified as essential in substrate/product recognition and catalysis for class II aldolases. In contrast to these, both aldolases required subunits GatZ or KbaZ (420 amino acids) for full activity and for good in vivo and in vitro stability. The Z subunits alone did not show any aldolase activity. Close relatives of these new TagBP aldolases were found in several gram-negative and gram-positive bacteria, e.g., Streptomyces coelicolor.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Brinkkötter A,Shakeri-Garakani A,Lengeler JWdoi
10.1007/s00203-002-0406-6keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
410-9issue
5eissn
0302-8933issn
1432-072Xjournal_volume
177pub_type
杂志文章abstract：:Clostridium butyricum is widely used to produce organic solvents such as ethanol, butanol and acetone. We sequenced the entire genome of C. butyricum INCQS635 by using Ion Torrent technology. We found a high contribution of sequences assigned for carbohydrate subsystems (15-20 % of known sequences). Annotation based o...
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pub_type： 杂志文章,评审
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更新日期：2015-08-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446644
更新日期：1976-09-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00416970
更新日期：1976-10-11 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00492022
更新日期：1977-06-20 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00203-020-01941-9
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pub_type： 杂志文章
doi：10.1007/s002030100315
更新日期：2001-09-01 00:00:00
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更新日期：2007-01-01 00:00:00
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更新日期：2020-05-01 00:00:00
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更新日期：2012-01-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1007/BF00428592
更新日期：1977-05-13 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429338
更新日期：1977-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-10-01 00:00:00
