Abstract：
:Previous analyses of labelled clones of cells within the developing nervous system of the mouse have indicated that descendants are initially dispersed rostrocaudally followed by more local proliferation, which is consistent with the progressing node's contributing descendants from a resident population of progenitor cells as it advances caudally. Here we electroporated an expression vector encoding green fluorescent protein into the chicken embryo near Hensen's node to test and confirm the pattern inferred in the mouse. This provides a model in which a proliferative stem zone is maintained in the node by a localized signal; those cells that are displaced out of the stem zone go on to contribute to the growing axis. To test whether fibroblast growth factor (FGF) signalling could be involved in the maintenance of the stem zone, we co-electroporated a dominant-negative FGF receptor with a lineage marker, and found that it markedly alters the elongation of the spinal cord primordium. The results indicate that FGF receptor signalling promotes the continuous development of the posterior nervous system by maintaining presumptive neural progenitors in the region near Hensen's node. This offers a potential explanation for the mixed findings on FGF in the growth and patterning of the embryonic axis.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Mathis L,Kulesa PM,Fraser SEdoi
10.1038/35078535keywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
559-66issue
6eissn
1465-7392issn
1476-4679pii
35078535journal_volume
3pub_type
杂志文章abstract：:Induced pluripotent stem cells (iPSCs) are generated via the expression of the transcription factors OCT4 (also known as POU5F1), SOX2, KLF4 and cMYC (OSKM) in somatic cells. In contrast to murine naive iPSCs, conventional human iPSCs are in a more developmentally advanced state called primed pluripotency. Here, we re...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0254-5
更新日期：2019-02-01 00:00:00
abstract：:In some organs, adult stem cells are uniquely poised to serve as cancer cells of origin. It is unclear, however, whether tumorigenesis is influenced by the activation state of the adult stem cell. Hair follicle stem cells (HFSCs) act as cancer cells of origin for cutaneous squamous cell carcinoma and undergo defined c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2889
更新日期：2014-01-01 00:00:00
abstract：:Autophagy is characterized by the sequestration of bulk cytoplasm, including damaged proteins and organelles, and delivery of the cargo to lysosomes for degradation. Although the autophagic pathway is also linked to tumour suppression activity, the mechanism is not yet clear. Here we report that cytosolic FoxO1, a for...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2069
更新日期：2010-07-01 00:00:00
abstract：:Nitric oxide (NO) is a pleiotropic signalling molecule that binds to cytochrome c oxidase (complex IV) reversibly and in competition with oxygen. This action of NO has both physiological and pathophysiological consequences. Here we report that endogenously generated NO, which disrupts the respiratory chain, may cause ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1188
更新日期：2004-11-01 00:00:00
abstract：:To maintain genome function and stability, DNA sequence and its organization into chromatin must be duplicated during cell division. Understanding how entire chromosomes are copied remains a major challenge. Here, we use nascent chromatin capture (NCC) to profile chromatin proteome dynamics during replication in human...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2918
更新日期：2014-03-01 00:00:00
abstract：:Obesity occurs when excess energy accumulates in white adipose tissue (WAT), whereas brown adipose tissue (BAT), specialized for energy expenditure through thermogenesis, potently counteracts obesity. Factors that induce brown adipocyte commitment and energy expenditure would be a promising defence against adiposity. ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2867
更新日期：2013-12-01 00:00:00
abstract：:Microtubules are central elements of the eukaryotic cytoskeleton that often function as part of branched networks. Current models for branching include nucleation of new microtubules from severed microtubule seeds or from γ-tubulin recruited to the side of a pre-existing microtubule. Here, we found that microtubules c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0199-8
更新日期：2018-10-01 00:00:00
abstract：:Aurora A, an integral mitotic kinase, is essential for microtubule dynamics of post-mitotic neurons. PKCzeta activates Aurora A, which in turn phosphorylates NDEL1 to promote neurite extension. This raises the possibility that Aurora A may also be involved in establishing cell polarity and axon/dendrite elaboration in...
journal_title：Nature cell biology
pub_type： 评论,新闻
doi：10.1038/ncb0909-1053
更新日期：2009-09-01 00:00:00
abstract：:The substrates used to modify nucleic acids and chromatin are affected by nutrient availability and the activity of metabolic pathways. Thus, cellular metabolism constitutes a fundamental component of chromatin status and thereby of genome regulation. Here we describe the biochemical and genetic principles of how meta...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb3629
更新日期：2017-11-01 00:00:00
abstract：:Numerous cellular functions depend on actin filament (F-actin) disassembly. The best-characterized disassembly proteins, the ADF (actin-depolymerizing factor)/cofilins (encoded by the twinstar gene in Drosophila), sever filaments and recycle monomers to promote actin assembly. Cofilin is also a relatively weak actin d...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3390
更新日期：2016-08-01 00:00:00
abstract：:The precise regulation of growth factor signalling is crucial to the molecular control of development in Drosophila. Post-translational modification of signalling molecules is one of the mechanisms that modulate developmental signalling specificity. We describe a new gene, fringe connection (frc), that encodes a nucle...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb0901-809
更新日期：2001-09-01 00:00:00
abstract：:Cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-activated chloride channel expressed in a wide variety of epithelial cells, mutations of which are responsible for the hallmark defective chloride secretion observed in cystic fibrosis (CF). Although CFTR has been implicated in bicarbonate secretion,...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1047
更新日期：2003-10-01 00:00:00
abstract：:In Huntington disease, polyglutamine expansion of the protein huntingtin (Htt) leads to selective neurodegenerative loss of medium spiny neurons throughout the striatum by an unknown apoptotic mechanism. Binding of Hip-1, a protein normally associated with Htt, is reduced by polyglutamine expansion. Free Hip-1 binds t...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb735
更新日期：2002-02-01 00:00:00
abstract：:Assisted reproductive technologies (ART) are exceptional among clinical therapies, as unlike most medical procedures, ART have generational consequences. Further, human embryo research in the US has been sponsored solely by the private sector and, until recent biotechnology forays into human embryonic stem cell (hESC)...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb-nm-fertilityS19
更新日期：2002-10-01 00:00:00
abstract：:Although myosin II filaments are known to exist in non-muscle cells, their dynamics and organization are incompletely understood. Here, we combined structured illumination microscopy with pharmacological and genetic perturbations, to study the process of actomyosin cytoskeleton self-organization into arcs and stress f...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3466
更新日期：2017-02-01 00:00:00
abstract：:The target of rapamycin complex 2 (TORC2) plays a key role in maintaining the homeostasis of plasma membrane (PM) tension. TORC2 activation following increased PM tension involves redistribution of the Slm1 and 2 paralogues from PM invaginations known as eisosomes into membrane compartments containing TORC2. How Slm1/...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0150-z
更新日期：2018-09-01 00:00:00
abstract：:When cells enter mitosis with DNA that is unfit to be segregated, the consequences appear to be loss of centrosome function, abnormal spindles and a failure to segregate chromosomes. These defects may result from the workings of a surveillance mechanism that acts to cull irreparable nuclei. ...
journal_title：Nature cell biology
pub_type： 评论,新闻
doi：10.1038/35000099
更新日期：2000-02-01 00:00:00
abstract：:The cyclical protrusion and retraction of the leading edge is a hallmark of many migrating cells involved in processes such as development, inflammation and tumorigenesis. The molecular identity of the signalling mechanisms that control these cycles has remained unknown. Here, we used live-cell imaging of biosensors t...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2231
更新日期：2011-06-01 00:00:00
abstract：:Phosphoinositides (PtdIns) control fundamental cell processes, and inherited defects of PtdIns kinases or phosphatases cause severe human diseases, including Lowe syndrome due to mutations in OCRL, which encodes a PtdIns(4,5)P2 5-phosphatase. Here we unveil a lysosomal response to the arrival of autophagosomal cargo i...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3386
更新日期：2016-08-01 00:00:00
abstract：:Osteoclasts are multinucleated cells of the monocyte/macrophage lineage that degrade bone. Here, we used lineage tracing studies-labelling cells expressing Cx3cr1, Csf1r or Flt3-to identify the precursors of osteoclasts in mice. We identified an erythromyeloid progenitor (EMP)-derived osteoclast precursor population. ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-019-0437-8
更新日期：2020-01-01 00:00:00
abstract：:In chromaffin cells, exocytosis of single granules and properties of the fusion pore--the first connection between vesicular lumen and extracellular space --can be studied by cell-attached patch amperometry, which couples patch-clamp capacitance measurements with simultaneous amperometric recordings of transmitter rel...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb956
更新日期：2003-04-01 00:00:00
abstract：:Heterochromatin performs a central role in chromosome segregation and stability by promoting cohesion at centromeres. Establishment of both heterochromatin-mediated silencing and cohesion requires passage through S phase, although the mechanism is unknown. Here we demonstrate that Schizosaccharomyces pombe Hsk1 (CDC7)...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1069
更新日期：2003-12-01 00:00:00
abstract：:The intracellular Ca(2+) concentration ([Ca(2+)](i)) in skeletal muscles must be rapidly regulated during the excitation-contraction-relaxation process. However, the signalling components involved in such rapid Ca(2+) movement are not fully understood. Here we report that mice deficient in the newly identified PtdInsP...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1884
更新日期：2009-06-01 00:00:00
abstract：:Formins have been implicated in the regulation of cytoskeletal structure in animals and fungi. Here we show that the formins Bni1 and Bnr1 of budding yeast stimulate the assembly of actin filaments that function as precursors to tropomyosin-stabilized cables that direct polarized cell growth. With loss of formin funct...
journal_title：Nature cell biology
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1038/ncb770
更新日期：2002-03-01 00:00:00
abstract：:The serine/threonine protein kinase Akt is involved in a variety of cellular processes including cell proliferation, survival, metabolism and gene expression. It is essential in vascular endothelial growth factor (VEGF)-mediated angiogenesis; however, it is not known how Akt regulates the migration of endothelial cell...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1695
更新日期：2008-03-01 00:00:00
abstract：:Etk/BMX, a member of the Btk family of tyrosine kinases, is highly expressed in cells with great migratory potential, including endothelial cells and metastatic carcinoma cell lines. Here, we present evidence that Etk is involved in integrin signalling and promotes cell migration. The activation of Etk by extracellula...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35074500
更新日期：2001-05-01 00:00:00
abstract：:Junior researchers are encouraged to gain experience abroad, and for senior scientists, sabbaticals remain popular. France has taken the next step in fostering international exchange, by supporting long-term collaborations with foreign laboratories and by creating research units abroad. ...
journal_title：Nature cell biology
pub_type： 社论
doi：10.1038/ncb0607-613
更新日期：2007-06-01 00:00:00
abstract：:Although normally dormant, hair follicle stem cells (HFSCs) quickly become activated to divide during a new hair cycle. The quiescence of HFSCs is known to be regulated by a number of intrinsic and extrinsic mechanisms. Here we provide several lines of evidence to demonstrate that HFSCs utilize glycolytic metabolism a...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3575
更新日期：2017-09-01 00:00:00
abstract：:Focal adhesions are integrin-based multiprotein complexes, several micrometres in diameter, that mechanically link the extracellular matrix with the termini of actin bundles. The molecular diversity of focal adhesions and their role in cell migration and matrix sensing has been extensively studied, but their ultrastru...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2095
更新日期：2010-09-01 00:00:00
abstract：:Mitochondrial DNA (mtDNA) mutations cause inherited diseases and are implicated in the pathogenesis of common late-onset disorders, but how they arise is not clear1,2. Here we show that mtDNA mutations are present in primordial germ cells (PGCs) within healthy female human embryos. Isolated PGCs have a profound reduct...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-017-0017-8
更新日期：2018-02-01 00:00:00
