Insulin inhibits transcription of IRS-2 gene in rat liver through an insulin response element (IRE) that resembles IREs of other insulin-repressed genes.

abstract：:A calix[4]arene was designed to reversibly dimerize and form an egg-shaped enclosure. Adhesive interactions in the assembly were provided by four self-associating ureas, which form a cyclic array containing 16 hydrogen bonds. The synthesis was completed in four steps from the previously described O,O',O",O"'-tetrabenz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shimizu KD,Rebek J Jr

更新日期：1995-12-19 00:00:00

abstract：:Analysis of the nucleotide and deduced amino acid sequences of the biologically active mouse mdr1 cDNA clone indicates that the protein is formed by two highly homologous halves, each containing six putative transmembrane domains and a nucleotide-binding site. The duplicated unit shows high sequence homology to the pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raymond M,Gros P

更新日期：1989-09-01 00:00:00

abstract：:Block of Ca2+ currents by the dihydropyridine drug nitrendipine was studied in single canine ventricular cells by using the whole-cell variant of the patch clamp technique. When cells were held at depolarized membrane potentials at which Ca2+ currents were approximately equal to 70% inactivated, nitrendipine blocked C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bean BP

更新日期：1984-10-01 00:00:00

abstract：:The region of human chromosome 22q11 is prone to rearrangements. The resulting chromosomal abnormalities are involved in Velo-cardio-facial and DiGeorge syndromes (VCFS and DGS) (deletions), "cat eye" syndrome (duplications), and certain types of tumors (translocations). As a prelude to the development of mouse models...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Puech A,Saint-Jore B,Funke B,Gilbert DJ,Sirotkin H,Copeland NG,Jenkins NA,Kucherlapati R,Morrow B,Skoultchi AI

更新日期：1997-12-23 00:00:00

abstract：:The 26S proteasome, a molecular machine responsible for regulated protein degradation, consists of a proteolytic core particle (20S CP) associated with 19S regulatory particles (19S RPs) subdivided into base and lid subcomplexes. The assembly of 19S RP base subcomplex is mediated by multiple dedicated chaperones. Amon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barrault MB,Richet N,Godard C,Murciano B,Le Tallec B,Rousseau E,Legrand P,Charbonnier JB,Le Du MH,Guérois R,Ochsenbein F,Peyroche A

更新日期：2012-04-24 00:00:00

abstract：:We have constructed a phylogenic tree for green plants by comparing 5S rRNA sequences. The tree suggests that the emergence of most of the uni- and multicellular green algae such as Chlamydomonas, Spirogyra, Ulva, and Chlorella occurred in the early stage of green plant evolution. The branching point of Nitella is a l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hori H,Lim BL,Osawa S

更新日期：1985-02-01 00:00:00

abstract：:CR1 is a short interspersed repetitive DNA element originally identified in the domestic chicken (Gallus gallus). However, unlike virtually all other such sequences described to date, CR1 is not confined to one or a few closely related species. It is probably a ubiquitous component of the avian genome, having been det...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen ZQ,Ritzel RG,Lin CC,Hodgetts RB

更新日期：1991-07-01 00:00:00

abstract：:We performed the first genome-wide expression analysis directly comparing the expression profile of highly enriched normal human hematopoietic stem cells (HSC) and leukemic stem cells (LSC) from patients with acute myeloid leukemia (AML). Comparing the expression signature of normal HSC to that of LSC, we identified 3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Majeti R,Becker MW,Tian Q,Lee TL,Yan X,Liu R,Chiang JH,Hood L,Clarke MF,Weissman IL

更新日期：2009-03-03 00:00:00

abstract：:Murine monoclonal antibodies that bind selectively to adducts formed on DNA by the antitumor drug cis-diamminedichloroplatinum(II), cis-DDP, or to the chemotherapeutically inactive trans isomer trans-DDP were elicited by immunization with calf thymus DNA modified with either cis- or trans-DDP at ratios of bound platin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sundquist WI,Lippard SJ,Stollar BD

更新日期：1987-12-01 00:00:00

abstract：:Sarcoplasmic reticulum vesicles were exposed to various thiol-directed spin labels, and the position of the label on the inner or outer vesicle surface was investigated as a function of the ATPase (adenosinetriphosphatase; ATP phosphohydrolase, EC 3.6.1.3) chemical state. Previous measurements of label accessibility t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Champeil P,Rigaud JL,Gary-Bobo CM

更新日期：1980-05-01 00:00:00

abstract：:Bacteriophage lambda integrates into the chromosome of its Escherichia coli host by means of a site-specific recombination between a locus on the phage chromosome (phage att site) and a locus on the bacterial chromosome (bacterial att site). The nucleotide sequence of four lambda att sites altered in site-specific rec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoess RH,Landy A

更新日期：1978-11-01 00:00:00

abstract：:In nonneuronal cells, several plasma membrane proteins such as exofacial enzymes, receptors, and ion channels recycle between their intracellular compartment(s) and the cell surface via an endosomal pathway. In neurons, however, this pathway has not been extensively characterized. In particular, it remains unclear whe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thoidis G,Chen P,Pushkin AV,Vallega G,Leeman SE,Fine RE,Kandror KV

更新日期：1998-01-06 00:00:00

abstract：:Interaction of the estrogen receptor/ligand complex with a DNA estrogen response element is known to regulate gene transcription. In turn, specific conformations of the receptor-ligand complex have been postulated to influence unique subsets of estrogen-responsive genes resulting in differential modulation and, ultima...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grese TA,Sluka JP,Bryant HU,Cullinan GJ,Glasebrook AL,Jones CD,Matsumoto K,Palkowitz AD,Sato M,Termine JD,Winter MA,Yang NN,Dodge JA

更新日期：1997-12-09 00:00:00

abstract：:RNA from noninfectious virions produced by two established clonal lines of sarcoma positive-leukemia negative (S+L-)-transformed 3T3 cells has been characterized. RNA from virions or nucleoids of S+L--(C243) cells consisted of three to four sizes: +/-44 S (6%), 28 S (17%), 18 S (38%), and <18 S (39%). 28S virion RNA c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Phillips LA,Hollis VW Jr,Bassin RH,Fischinger PJ

更新日期：1973-10-01 00:00:00

abstract：:The N-methyl-d-aspartate (NMDA) glutamate receptor (NMDAR), long implicated in developmental plasticity, shows decay time kinetics that shorten postnatally as NR2A subunits are added to the receptor. Neither the mechanism nor immediate effect of this change is known. We studied developing NMDAR currents by using visua...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Townsend M,Yoshii A,Mishina M,Constantine-Paton M

更新日期：2003-02-04 00:00:00

abstract：:Natural killer (NK) cells are key players in the innate response to viruses, including herpesviruses. In particular, the variety of viral strategies to modulate the recognition of certain herpesviruses witnesses the importance of NK cells in the control of this group of viruses. Still, NK evasion strategies have remai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grauwet K,Cantoni C,Parodi M,De Maria A,Devriendt B,Pende D,Moretta L,Vitale M,Favoreel HW

更新日期：2014-11-11 00:00:00

abstract：:Because of the insolubility and polymeric properties of amyloid fibrils, techniques used conventionally to analyze protein structure and dynamics have often been hampered. Ultrasonication can induce the monomeric solution of amyloidogenic proteins to form amyloid fibrils. However, ultrasonication can break down prefor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chatani E,Lee YH,Yagi H,Yoshimura Y,Naiki H,Goto Y

更新日期：2009-07-07 00:00:00

abstract：:Several overlapping chromosomal deletions spanning the albino locus in the mouse cause perinatal lethality when homozygous and a block in the transcriptional induction of various unlinked hepatocyte-specific genes. Studies of such lethal albino deletion homozygotes in perinatal stages revealed a deficiency in the tran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zaret KS,Milos P,Lia M,Bali D,Gluecksohn-Waelsch S

更新日期：1992-07-15 00:00:00

abstract：:Drosophila ovo/svb (dovo) is required for epidermal cuticle/denticle differentiation and is genetically downstream of the wg signaling pathway. Similarly, a mouse homolog of dovo, movo1, is required for the proper formation of hair, a mammalian epidermal appendage. Here, we provide biochemical evidence that movo1 enco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li B,Mackay DR,Dai Q,Li TW,Nair M,Fallahi M,Schonbaum CP,Fantes J,Mahowald AP,Waterman ML,Fuchs E,Dai X

更新日期：2002-04-30 00:00:00

abstract：:The structure of activated pig heart aconitase [citrate(isocitrate) hydro-lyase, EC 4.2.1.3] containing a [4Fe-4S] cluster has been refined at 2.5-A resolution to a crystallographic residual of 18.2%. Comparison of this structure to the recently determined 2.1-A resolution structure of the inactive enzyme containing a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robbins AH,Stout CD

更新日期：1989-05-01 00:00:00

abstract：:Bacterial RNases catalyze the turnover of RNA and are essential for gene expression and quality surveillance of transcripts. In Escherichia coli, the exoribonucleases RNase R and polynucleotide phosphorylase (PNPase) play critical roles in degrading RNA. Here, we developed an optical-trapping assay to monitor the tran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fazal FM,Koslover DJ,Luisi BF,Block SM

更新日期：2015-12-08 00:00:00

abstract：:The effects of calcium ion on the Na+ activation gate were studied in squid giant axons. Saxitoxin (STX) was used to block ion entry into Na+ channels without hindering access to the membrane surface, making it possible to distinguish surface effects of calcium from pore-occupancy effects. In the presence of STX, gati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Armstrong CM

更新日期：1999-03-30 00:00:00

abstract：:Phenotypic alterations induced by the cytokinin 6-benzylaminopurine in cell suspensions of Nicotiana plumbaginifolia were studied at the level of the NH(2)-terminal sequence of the constituent proteins. Total protein extracts were separated by classical two-dimensional PAGE, and the proteins were recovered by electrob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bauw G,De Loose M,Inzé D,Van Montagu M,Vandekerckhove J

更新日期：1987-07-01 00:00:00

abstract：:Interphotoreceptor retinoid-binding protein (IRBP) has been hypothesized to function as an intercellular shuttle in the vertebrate eye, serving to transport retinoids between the retinal pigment epithelium (RPE) and photoreceptors in the process by which visual pigment is regenerated after photolysis. This hypothesis ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okajima TI,Pepperberg DR,Ripps H,Wiggert B,Chader GJ

更新日期：1990-09-01 00:00:00

abstract：:Local changes in conformation between the calcium-saturated and calcium-free forms of calmodulin were monitored using antisera to four peptides corresponding to three helical regions of the calcium-saturated protein. The N-terminal helix was monitored using antiserum to residues 9-19, calmodulin-(9-19); the C-terminal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gariépy J,Mietzner TA,Schoolnik GK

更新日期：1986-12-01 00:00:00

abstract：:Periodicities in the polar/apolar character of the amino acid sequence of a protein can be examined by assigning to each residue a numerical hydrophobicity and searching for periodicity in the resulting one-dimensional function. The strength of each periodic component is the quantity that has been termed the hydrophob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eisenberg D,Weiss RM,Terwilliger TC

更新日期：1984-01-01 00:00:00

abstract：:The immunoglobulin light chain is thought to be organized into two structural domains, the constant and the variable regions, linked by a switch region of lesser tertiary organization. Light chains of homogeneous rabbit antibodies to pneumococcal polysaccharides labeled with (125)I were subjected to trypsin digestion ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poulsen K,Fraser KJ,Haber E

更新日期：1972-09-01 00:00:00

abstract：:An artificial charge pair buried in the hydrophobic core of staphylococcal nuclease was engineered by making the V23E and L36K substitutions. Buried individually, Glu-23 and Lys-36 both titrate with pKa values near 7. When buried together their pKa values appear to be normal. The ionizable moieties of the buried Glu-L...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robinson AC,Castañeda CA,Schlessman JL,García-Moreno EB

更新日期：2014-08-12 00:00:00

abstract：:The sparking of cut grape hemispheres in a household microwave oven has been a poorly explained Internet parlor trick for over two decades. By expanding this phenomenon to whole spherical dimers of various grape-sized fruit and hydrogel water beads, we demonstrate that the formation of plasma is due to electromagnetic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khattak HK,Bianucci P,Slepkov AD

更新日期：2019-03-05 00:00:00

abstract：:Bacterial chemotaxis receptors can detect a small concentration gradient of attractants and repellents in the environment over a wide range of background concentration. The clustering of these receptors to form patches observed in vivo and in vitro has been suspected as a reason for the high sensitivity, and such wide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim SH,Wang W,Kim KK

更新日期：2002-09-03 00:00:00