Abstract:
:Because of the insolubility and polymeric properties of amyloid fibrils, techniques used conventionally to analyze protein structure and dynamics have often been hampered. Ultrasonication can induce the monomeric solution of amyloidogenic proteins to form amyloid fibrils. However, ultrasonication can break down preformed fibrils into shorter fibrils. Here, combining these 2 opposing effects on beta(2)-microglobulin (beta2-m), a protein responsible for dialysis-related amyloidosis, we present that ultrasonication pulses are useful for preparing monodispersed amyloid fibrils of minimal size with an average molecular weight of approximately 1,660,000 (140-mer). The production of minimal and monodispersed fibrils is achieved by the free energy minimum under competition between fibril production and breakdown. The small homogeneous fibrils will be of use for characterizing the structure and dynamics of amyloid fibrils, advancing molecular understanding of amyloidosis.
journal_name
Proc Natl Acad Sci U S Aauthors
Chatani E,Lee YH,Yagi H,Yoshimura Y,Naiki H,Goto Ydoi
10.1073/pnas.0901422106subject
Has Abstractpub_date
2009-07-07 00:00:00pages
11119-24issue
27eissn
0027-8424issn
1091-6490pii
0901422106journal_volume
106pub_type
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