Protein-binding dynamics imaged in a living cell.

abstract：:Neither the structure nor the function of alpha-bungarotoxin (alpha Bgtx) binding molecules in the nervous system have yet been completely defined, although it is known that some of these molecules are related to cation channels and some are not. Using an improved method of affinity chromatography, we have isolated a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gotti C,Ogando AE,Hanke W,Schlue R,Moretti M,Clementi F

更新日期：1991-04-15 00:00:00

abstract：:The temporal dynamics of electrical activity in an olfactory organ, the procerebral lobe of the terrestrial mollusc Limax maximus, is studied. The lobe exhibits intrinsic oscillations in its field potential. Intracellular recordings show that the lobe contains two classes of neurons, both with activity phase-locked to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Delaney KR,Gelperin A,Fee MS,Flores JA,Gervais R,Tank DW,Kleinfeld D

更新日期：1994-01-18 00:00:00

abstract：:Artificial two-dimensional biological habitats were prepared from porous polymer layers and inoculated with the fungus Penicillium roqueforti to provide a living material. Such composites of classical industrial ingredients and living microorganisms can provide a novel form of functional or smart materials with capabi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerber LC,Koehler FM,Grass RN,Stark WJ

更新日期：2012-01-03 00:00:00

abstract：:In bacteria, the first two steps of gene expression-transcription and translation-are spatially and temporally coupled. Uncoupling may lead to the arrest of transcription through RNA polymerase backtracking, which interferes with replication forks, leading to DNA double-stranded breaks and genomic instability. How tra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stevenson-Jones F,Woodgate J,Castro-Roa D,Zenkin N

更新日期：2020-04-14 00:00:00

abstract：:Multiple steps of plant growth and development rely on rapid cell elongation during which secretory and endocytic trafficking via the trans-Golgi network (TGN) plays a central role. Here, we identify the ECHIDNA (ECH) protein from Arabidopsis thaliana as a TGN-localized component crucial for TGN function. ECH partiall...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gendre D,Oh J,Boutté Y,Best JG,Samuels L,Nilsson R,Uemura T,Marchant A,Bennett MJ,Grebe M,Bhalerao RP

更新日期：2011-05-10 00:00:00

abstract：:We report that diverse species of bacteria encode a type IB DNA topoisomerase that resembles vaccinia virus topoisomerase. Deinococcus radiodurans topoisomerase IB (DraTopIB), an exemplary member of this family, relaxes supercoiled DNA in the absence of a divalent cation or ATP. DraTopIB has a compact size (346 aa) an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krogh BO,Shuman S

更新日期：2002-02-19 00:00:00

abstract：:We have isolated a cDNA clone from a human fibroblast cDNA library that contains the entire protein-coding region of a 1.1-kilobase mRNA. This mRNA encodes a 284-amino acid tropomyosin, the primary structure of which most closely resembles smooth muscle tropomyosin. Thus, the expression of both 284-amino acid muscle-t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： MacLeod AR,Houlker C,Reinach FC,Smillie LB,Talbot K,Modi G,Walsh FS

更新日期：1985-12-01 00:00:00

abstract：:DNA chip technology enables simultaneous examination of how approximately 6,200 Saccharomyces cerevisiae gene transcript levels, representing the entire genome, respond to environmental change. By using chips bearing oligonucleotide arrays, we show that, after exposure to the alkylating agent methyl methanesulfonate, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jelinsky SA,Samson LD

更新日期：1999-02-16 00:00:00

abstract：:We have expressed a male-specific, pheromone-sensitive odorant receptor (OR), BmorOR1, from the silkworm moth Bombyx mori in an "empty neuron" housed in the ab3 sensilla of a Drosophila Deltahalo mutant. Single-sensillum recordings showed that the BmorOR1-expressing neurons in the transgenic flies responded to the B. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Syed Z,Ishida Y,Taylor K,Kimbrell DA,Leal WS

更新日期：2006-10-31 00:00:00

abstract：:Immune responses to several soluble antigens were compared between B6.C-H-2bm12 mutant and wild-type B6 mice by using a lymph node T-cell proliferation assay. B6.C-H-2bm12 mice failed to respond to beef insulin whereas other IA gene-controlled responses, such as response to poly(L-Tyr, L-Glu)--poly(DL-Ala, L-Lys) and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin CC,Rosenthal AS,Passmore HC,Hansen TH

更新日期：1981-10-01 00:00:00

abstract：:Lod scores provide a method to unify linkage tests based on identity by descent and identity in marker state while permitting selection of the most informative individuals through their disease-related phenotypes and markers in relatives. After parametric lods are reviewed, a nonparametric approach that depends on a s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morton NE

更新日期：1996-04-16 00:00:00

abstract：:Antigenic markers associated with the major internal protein of RNA tumor viruses of the C-type have proven extremely useful in natural history studies of these viruses. This protein possesses species-specific antigenic determinants, and, in the case of mammalian C-type viruses, the protein possesses crossreactive det...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gilden RV,Oroszlan S

更新日期：1972-04-01 00:00:00

abstract：:With the collapse of fisheries in many parts of the world causing widespread economic harm, attention is focused on a possible cause and remedy of fishery collapse. Economic theory for managing a renewable resource, such as a fishery, leads to an ecologically unstable equilibrium as difficult to maintain as balancing ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roughgarden J,Smith F

更新日期：1996-05-14 00:00:00

abstract：:Development of the nervous system in the amphibian embryo is initiated during gastrulation by an inductive interaction between chordamesoderm and dorsal ectoderm. The induced ectoderm forms the neural plate while uninduced ectoderm generates epidermis. We screened for genes activated during gastrulation and expressed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Richter K,Grunz H,Dawid IB

更新日期：1988-11-01 00:00:00

abstract：:The second critical endpoint in the basalt-H(2)O system was directly determined by a high-pressure and high-temperature X-ray radiography technique. We found that the second critical endpoint occurs at around 3.4 GPa and 770 °C (corresponding to a depth of approximately 100 km in a subducting slab), which is much shal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mibe K,Kawamoto T,Matsukage KN,Fei Y,Ono S

更新日期：2011-05-17 00:00:00

abstract：:MAC5 is a component of the conserved MOS4-associated complex. It plays critical roles in development and immunity. Here we report that MAC5 is required for microRNA (miRNA) biogenesis. MAC5 interacts with Serrate (SE), which is a core component of the microprocessor that processes primary miRNA transcripts (pri-miRNAs...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li S,Li M,Liu K,Zhang H,Zhang S,Zhang C,Yu B

更新日期：2020-09-22 00:00:00

abstract：:Endothelial cell growth factor (ECGF) binds specifically in vitro to membrane receptors present on the surface of several cell types, including murine and human endothelial cells and fibroblasts. Monoclonal antibodies prepared against ECGF that inhibit the mitogenic activity of the growth factor prevent receptor occup...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schreiber AB,Kenney J,Kowalski WJ,Friesel R,Mehlman T,Maciag T

更新日期：1985-09-01 00:00:00

abstract：:An increasing number of protein structures are found to encompass multiple folding nuclei, allowing their structures to be formed by several competing pathways. A typical example is the ribosomal protein S6, which comprises two folding nuclei (sigma1 and sigma2) defining two competing pathways in the folding energy la...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haglund E,Lind J,Oman T,Ohman A,Mäler L,Oliveberg M

更新日期：2009-12-22 00:00:00

abstract：:Gentamicin C complex from Micromonospora echinospora remains a globally important antibiotic, and there is revived interest in the semisynthesis of analogs that might show improved therapeutic properties. The complex consists of five components differing in their methylation pattern at one or more sites in the molecul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li S,Guo J,Reva A,Huang F,Xiong B,Liu Y,Deng Z,Leadlay PF,Sun Y

更新日期：2018-02-06 00:00:00

abstract：:One of the issues currently being debated in the ongoing discussion of the pros and cons of today's globalization concerns the effects of greater world trade as well as of the changes in technology on a country's internal distribution of income, especially on skilled versus unskilled wage rates. In this article, I att...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jones RW

更新日期：2003-09-16 00:00:00

abstract：:GB virus B (GBV-B), which infects tamarins, is the virus most closely related to hepatitis C virus (HCV). HCV has a protein (p7) that is believed to form an ion channel. It is critical for viability. In vitro studies suggest that GBV-B has an analogous but larger protein (p13). We found that substitutions of the -1 an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takikawa S,Engle RE,Emerson SU,Purcell RH,St Claire M,Bukh J

更新日期：2006-02-28 00:00:00

abstract：:SUMO, a small ubiquitin-related modifier, is known to covalently attach to a number of nuclear regulatory proteins such as p53, IkappaB, promyelocytic leukemia protein and c-Jun. The sumoylation reaction is catalyzed by the SUMO protease, which exposes the C-terminal active glycine residue of the nascent SUMO, the het...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li T,Evdokimov E,Shen RF,Chao CC,Tekle E,Wang T,Stadtman ER,Yang DC,Chock PB

更新日期：2004-06-08 00:00:00

abstract：:Myosin V, a double-headed molecular motor, transports organelles within cells by walking processively along actin, a process that requires coordination between the heads. To understand the mechanism underlying this coordination, processive runs of single myosin V molecules were perturbed by varying nucleotide content....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baker JE,Krementsova EB,Kennedy GG,Armstrong A,Trybus KM,Warshaw DM

更新日期：2004-04-13 00:00:00

abstract：:The early steps in the biosynthesis of Taxol involve the cyclization of geranylgeranyl diphosphate to taxa-4(5),11(12)-diene followed by cytochrome P450-mediated hydroxylation at C5, acetylation of this intermediate, and a second cytochrome P450-dependent hydroxylation at C10 to yield taxadien-5 alpha-acetoxy-10 beta-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schoendorf A,Rithner CD,Williams RM,Croteau RB

更新日期：2001-02-13 00:00:00

abstract：:Phylogenetic analysis of Plasmodium parasites has indicated that their modern-day distribution is a result of a series of human-mediated dispersals involving transport between Africa, Europe, America, and Asia. A major outstanding question is the phylogenetic affinity of the malaria causing parasites Plasmodium vivax ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gelabert P,Sandoval-Velasco M,Olalde I,Fregel R,Rieux A,Escosa R,Aranda C,Paaijmans K,Mueller I,Gilbert MT,Lalueza-Fox C

更新日期：2016-10-11 00:00:00

abstract：:Structural symmetry is a hallmark of homomeric ion channels. Nonobligatory regulatory proteins can also critically define the precise functional role of such channels. For instance, the pore-forming subunit of the large conductance voltage and calcium-activated potassium (BK, Slo1, or KCa1.1) channels encoded by a sin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gonzalez-Perez V,Ben Johny M,Xia XM,Lingle CJ

更新日期：2018-10-02 00:00:00

abstract：:Excess activation of glutamate receptors and production of free radicals including nitric oxide may result in severe and irreversible damage to the mammalian central nervous system (CNS), but endogenous defense systems that protect neurons from these insults are poorly understood. Here, we purified and isolated a neur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kume T,Asai N,Nishikawa H,Mano N,Terauchi T,Taguchi R,Shirakawa H,Osakada F,Mori H,Asakawa N,Yonaga M,Nishizawa Y,Sugimoto H,Shimohama S,Katsuki H,Kaneko S,Akaike A

更新日期：2002-03-05 00:00:00

abstract：:RNA from noninfectious virions produced by two established clonal lines of sarcoma positive-leukemia negative (S+L-)-transformed 3T3 cells has been characterized. RNA from virions or nucleoids of S+L--(C243) cells consisted of three to four sizes: +/-44 S (6%), 28 S (17%), 18 S (38%), and <18 S (39%). 28S virion RNA c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Phillips LA,Hollis VW Jr,Bassin RH,Fischinger PJ

更新日期：1973-10-01 00:00:00

abstract：:Polymorphonuclear leukocytes isolated from chicken peritoneal exudates have been found to catalyze cyanide-insensitive stimulation of respiration and the hexose monophosphate shunt upon exposure to heat-inactivated Staphylococcus aureus. However, there was no demonstrable formate oxidation concomitant with phagocytosi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Penniall R,Spitznagel JK

更新日期：1975-12-01 00:00:00

abstract：:Recently, the focus of network research shifted to network controllability, prompting us to determine proteins that are important for the control of the underlying interaction webs. In particular, we determined minimum dominating sets of proteins (MDSets) in human and yeast protein interaction networks. Such groups of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wuchty S

更新日期：2014-05-13 00:00:00