Conserved Arabidopsis ECHIDNA protein mediates trans-Golgi-network trafficking and cell elongation.

Abstract:

:Multiple steps of plant growth and development rely on rapid cell elongation during which secretory and endocytic trafficking via the trans-Golgi network (TGN) plays a central role. Here, we identify the ECHIDNA (ECH) protein from Arabidopsis thaliana as a TGN-localized component crucial for TGN function. ECH partially complements loss of budding yeast TVP23 function and a Populus ECH complements the Arabidopsis ech mutant, suggesting functional conservation of the genes. Compared with wild-type, the Arabidopsis ech mutant exhibits severely perturbed cell elongation as well as defects in TGN structure and function, manifested by the reduced association between Golgi bodies and TGN as well as mislocalization of several TGN-localized proteins including vacuolar H(+)-ATPase subunit a1 (VHA-a1). Strikingly, ech is defective in secretory trafficking, whereas endocytosis appears unaffected in the mutant. Some aspects of the ech mutant phenotype can be phenocopied by treatment with a specific inhibitor of vacuolar H(+)-ATPases, concanamycin A, indicating that mislocalization of VHA-a1 may account for part of the defects in ech. Hence, ECH is an evolutionarily conserved component of the TGN with a central role in TGN structure and function.

authors

Gendre D,Oh J,Boutté Y,Best JG,Samuels L,Nilsson R,Uemura T,Marchant A,Bennett MJ,Grebe M,Bhalerao RP

doi

10.1073/pnas.1018371108

subject

Has Abstract

pub_date

2011-05-10 00:00:00

pages

8048-53

issue

19

eissn

0027-8424

issn

1091-6490

pii

1018371108

journal_volume

108

pub_type

杂志文章
  • Enhancement of hexose entry into chick fibroblasts by starvation: differential effect on galactose and glucose.

    abstract::Glucose entry, as measured by 5-min uptake into the acid-soluble fraction, is enhanced 15-30 times by long-term (12-24 hr) hexose starvation of chick fibroblasts. The rate of galactose accumulation in the cells increases only 5 times under the same conditions of starvation. Several carbon and energy sources that were ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.69.11.3407

    authors: Martineau R,Kohlbacher M,Shaw SN,Amos H

    更新日期:1972-11-01 00:00:00

  • Transcriptome-wide analysis of PGC-1α-binding RNAs identifies genes linked to glucagon metabolic action.

    abstract::The peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) is a transcriptional coactivator that controls expression of metabolic/energetic genes, programming cellular responses to nutrient and environmental adaptations such as fasting, cold, or exercise. Unlike other coactivators, PGC-1α contai...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2000643117

    authors: Tavares CDJ,Aigner S,Sharabi K,Sathe S,Mutlu B,Yeo GW,Puigserver P

    更新日期:2020-09-08 00:00:00

  • Discovery and characterization of an epithelial-specific galectin in the endometrium that forms crystals in the trophectoderm.

    abstract::Secretions of the uterus support survival and growth of the conceptus (embryo/fetus and associated membranes) during pregnancy. Galectin-15, also known as OVGAL11 and a previously uncharacterized member of the galectin family of secreted beta-galactoside lectins containing a conserved carbohydrate recognition domain a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0402669101

    authors: Gray CA,Adelson DL,Bazer FW,Burghardt RC,Meeusen EN,Spencer TE

    更新日期:2004-05-25 00:00:00

  • Cross-subunit interactions that stabilize open states mediate gating in NMDA receptors.

    abstract::NMDA receptors are excitatory channels with critical functions in the physiology of central synapses. Their activation reaction proceeds as a series of kinetically distinguishable, reversible steps, whose structural bases are currently under investigation. Very likely, the earliest steps include glutamate binding to g...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2007511118

    authors: Iacobucci GJ,Wen H,Helou M,Liu B,Zheng W,Popescu GK

    更新日期:2021-01-12 00:00:00

  • Symmetry in chemistry from the hydrogen atom to proteins.

    abstract::The last 2 decades have seen discoveries in highly excited states of atoms and molecules of phenomena that are qualitatively different from the "planetary" model of the atom, and the near-rigid model of molecules, characteristic of these systems in their low-energy states. A unified view is emerging in terms of approx...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.25.14287

    authors: Kellman ME

    更新日期:1996-12-10 00:00:00

  • Illuminating spatial A-to-I RNA editing signatures within the Drosophila brain.

    abstract::Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, is a ubiquitous mechanism that generates transcriptomic diversity. This process is particularly important for proper neuronal function; however, little is known about how RNA editing is dynamically regulated between the many functionally distinct ne...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1811768116

    authors: Sapiro AL,Shmueli A,Henry GL,Li Q,Shalit T,Yaron O,Paas Y,Billy Li J,Shohat-Ophir G

    更新日期:2019-02-05 00:00:00

  • Treatment of a human breast cancer xenograft with an adenovirus vector containing an interferon gene results in rapid regression due to viral oncolysis and gene therapy.

    abstract::Treatment of a human breast cancer cell line (MDA-MB-435) in nude mice with a recombinant adenovirus containing the human interferon (IFN) consensus gene, IFN-con1 (ad5/IFN), resulted in tumor regression in 100% of the animals. Tumor regression occurred when virus was injected either within 24 hr of tumor cell implant...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.9.4513

    authors: Zhang JF,Hu C,Geng Y,Selm J,Klein SB,Orazi A,Taylor MW

    更新日期:1996-04-30 00:00:00

  • Anatomy of funded research in science.

    abstract::Seeking research funding is an essential part of academic life. Funded projects are primarily collaborative in nature through internal and external partnerships, but what role does funding play in the formulation of these partnerships? Here, by examining over 43,000 scientific projects funded over the past three decad...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1513651112

    authors: Ma A,Mondragón RJ,Latora V

    更新日期:2015-12-01 00:00:00

  • Poor prognosis in carcinoma is associated with a gene expression signature of aberrant PTEN tumor suppressor pathway activity.

    abstract::Pathway-specific therapy is the future of cancer management. The oncogenic phosphatidylinositol 3-kinase (PI3K) pathway is frequently activated in solid tumors; however, currently, no reliable test for PI3K pathway activation exists for human tumors. Taking advantage of the observation that loss of PTEN, the negative ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0702507104

    authors: Saal LH,Johansson P,Holm K,Gruvberger-Saal SK,She QB,Maurer M,Koujak S,Ferrando AA,Malmström P,Memeo L,Isola J,Bendahl PO,Rosen N,Hibshoosh H,Ringnér M,Borg A,Parsons R

    更新日期:2007-05-01 00:00:00

  • Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression.

    abstract::An essential step in the pathogenesis of human papillomavirus (HPV)-associated cancers is the dysregulated expression of the viral oncogenes. The papillomavirus E2 protein can silence the long control region (LCR) promoter that controls viral E6 and E7 oncogene expression. The mechanisms by which E2 represses oncogene...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0914818107

    authors: Smith JA,White EA,Sowa ME,Powell ML,Ottinger M,Harper JW,Howley PM

    更新日期:2010-02-23 00:00:00

  • Conformation of lysine vasopressin: a comparison with oxytocin.

    abstract::Starting with assignments of proton nuclear magnetic resonance previously made for oxytocin in deuterated dimethylsulfoxide at 220 MHz, we have assigned resonances for the mammalian antidiuretic hormone, lysine vasopressin. The results demonstrate that spectral assignments of neurohypophyseal hormones and their congen...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.69.7.1920

    authors: Walter R,Glickson JD,Schwartz IL,Havran RT,Meienhofer J,Urry DW

    更新日期:1972-07-01 00:00:00

  • The spread of attention across modalities and space in a multisensory object.

    abstract::Attending to a stimulus is known to enhance the neural responses to that stimulus. Recent experiments on visual attention have shown that this modulation can have object-based characteristics, such that, when certain parts of a visual object are attended, other parts automatically also receive enhanced processing. Her...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0507704102

    authors: Busse L,Roberts KC,Crist RE,Weissman DH,Woldorff MG

    更新日期:2005-12-20 00:00:00

  • Evidence that DNA is present in abnormal tubulofilamentous structures found in scrapie.

    abstract::Abnormal tubulofilamentous structures have been identified in electron micrographs of thin sections and negatively stained impression grids prepared from brains of animals with scrapie and other spongiform encephalopathies, and we showed that such tubules contain a core of filamentous structures resembling scrapie-ass...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.10.3575

    authors: Narang HK,Asher DM,Gajdusek DC

    更新日期:1988-05-01 00:00:00

  • Syndecan-4 proteoliposomes enhance fibroblast growth factor-2 (FGF-2)-induced proliferation, migration, and neovascularization of ischemic muscle.

    abstract::Ischemia of the myocardium and lower limbs is a common consequence of arterial disease and a major source of morbidity and mortality in modernized countries. Inducing neovascularization for the treatment of ischemia is an appealing therapeutic strategy for patients for whom traditional treatment modalities cannot be p...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1117885109

    authors: Jang E,Albadawi H,Watkins MT,Edelman ER,Baker AB

    更新日期:2012-01-31 00:00:00

  • Human HA and polymerase subunit PB2 proteins confer transmission of an avian influenza virus through the air.

    abstract::The influenza virus genes that confer efficient transmission of epidemic and pandemic strains in humans have not been identified. The rapid spread and severe disease caused by the 1918 influenza pandemic virus makes it an ideal virus to study the transmissibility of potentially pandemic influenza strains. Here, we use...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0813172106

    authors: Van Hoeven N,Pappas C,Belser JA,Maines TR,Zeng H,García-Sastre A,Sasisekharan R,Katz JM,Tumpey TM

    更新日期:2009-03-03 00:00:00

  • A fluorescence-based assay for monitoring helicase activity.

    abstract::A continuous fluorescence-based assay is described for measuring helicase-mediated unwinding of duplex DNA. The assay utilizes an oligonucleotide substrate containing the fluorescent adenine analog, 2-aminopurine, at regular intervals. 2-Aminopurine forms a Watson-Crick-type base pair with thymine and does not distort...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.14.6644

    authors: Raney KD,Sowers LC,Millar DP,Benkovic SJ

    更新日期:1994-07-05 00:00:00

  • Zinc folds the N-terminal domain of HIV-1 integrase, promotes multimerization, and enhances catalytic activity.

    abstract::The N-terminal domain of HIV-1 integrase contains a pair of His and Cys residues (the HHCC motif) that are conserved among retroviral integrases. Although His and Cys residues are often involved in binding zinc, the HHCC motif does not correspond to any recognized class of zinc binding domain. We have investigated the...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.24.13659

    authors: Zheng R,Jenkins TM,Craigie R

    更新日期:1996-11-26 00:00:00

  • Top performers are not the most impressive when extreme performance indicates unreliability.

    abstract::The relationship between performance and ability is a central concern in the social sciences: Are the most successful much more able than others, and are failures unskilled? Prior research has shown that noise and self-reinforcing dynamics make performance unpredictable and lead to a weak association between ability a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1116048109

    authors: Denrell J,Liu C

    更新日期:2012-06-12 00:00:00

  • T-box transcription factor 3 governs a transcriptional program for the function of the mouse atrioventricular conduction system.

    abstract::Genome-wide association studies have identified noncoding variants near TBX3 that are associated with PR interval and QRS duration, suggesting that subtle changes in TBX3 expression affect atrioventricular conduction system function. To explore whether and to what extent the atrioventricular conduction system is affec...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1919379117

    authors: Mohan RA,Bosada FM,van Weerd JH,van Duijvenboden K,Wang J,Mommersteeg MTM,Hooijkaas IB,Wakker V,de Gier-de Vries C,Coronel R,Boink GJJ,Bakkers J,Barnett P,Boukens BJ,Christoffels VM

    更新日期:2020-08-04 00:00:00

  • Hepatocyte growth factor and macrophage inflammatory protein 1 beta: structurally distinct cytokines that induce rapid cytoskeletal changes and subset-preferential migration in T cells.

    abstract::T-cell migration into tissue depends on a cascade of rapid and selective adhesive interactions with endothelium. "Triggering" is a step in that cascade required to activate T-cell integrins. Hepatocyte growth factor (HGF) may be a physiologically relevant trigger, since we demonstrate that HGF can induce both adhesion...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.15.7144

    authors: Adams DH,Harvath L,Bottaro DP,Interrante R,Catalano G,Tanaka Y,Strain A,Hubscher SG,Shaw S

    更新日期:1994-07-19 00:00:00

  • Evolution of antifreeze glycoprotein gene from a trypsinogen gene in Antarctic notothenioid fish.

    abstract::Freezing avoidance conferred by different types of antifreeze proteins in various polar and subpolar fishes represents a remarkable example of cold adaptation, but how these unique proteins arose is unknown. We have found that the antifreeze glycoproteins (AFGPs) of the predominant Antarctic fish taxon, the notothenio...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.94.8.3811

    authors: Chen L,DeVries AL,Cheng CH

    更新日期:1997-04-15 00:00:00

  • Structure-based ligand discovery for the protein-protein interface of chemokine receptor CXCR4.

    abstract::G-protein-coupled receptors (GPCRs) are key signaling molecules and are intensely studied. Whereas GPCRs recognizing small-molecules have been successfully targeted for drug discovery, protein-recognizing GPCRs, such as the chemokine receptors, claim few drugs or even useful small molecule reagents. This reflects both...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1120431109

    authors: Mysinger MM,Weiss DR,Ziarek JJ,Gravel S,Doak AK,Karpiak J,Heveker N,Shoichet BK,Volkman BF

    更新日期:2012-04-03 00:00:00

  • Identifying the fundamental units of bacterial diversity: a paradigm shift to incorporate ecology into bacterial systematics.

    abstract::The central questions of bacterial ecology and evolution require a method to consistently demarcate, from the vast and diverse set of bacterial cells within a natural community, the groups playing ecologically distinct roles (ecotypes). Because of a lack of theory-based guidelines, current methods in bacterial systema...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0712205105

    authors: Koeppel A,Perry EB,Sikorski J,Krizanc D,Warner A,Ward DM,Rooney AP,Brambilla E,Connor N,Ratcliff RM,Nevo E,Cohan FM

    更新日期:2008-02-19 00:00:00

  • Element-specific localization of Drosophila retrotransposon Gag proteins occurs in both nucleus and cytoplasm.

    abstract::Many Drosophila non-long terminal repeat (LTR) retrotransposons actively transpose into internal, gene-rich regions of chromosomes but do not transpose onto chromosome ends. HeT-A and TART are remarkable exceptions; they form telomeres of Drosophila by repeated transpositions onto the ends of chromosomes and never tra...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.032071999

    authors: Rashkova S,Karam SE,Pardue ML

    更新日期:2002-03-19 00:00:00

  • Structural basis for peptidoglycan binding by peptidoglycan recognition proteins.

    abstract::Peptidoglycan (PGN) recognition proteins (PGRPs) are pattern-recognition receptors of the innate immune system that bind and, in some cases, hydrolyze bacterial PGNs. We determined the crystal structure, at 2.30-A resolution, of the C-terminal PGN-binding domain of human PGRP-Ialpha in complex with a muramyl tripeptid...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0407856101

    authors: Guan R,Roychowdhury A,Ember B,Kumar S,Boons GJ,Mariuzza RA

    更新日期:2004-12-07 00:00:00

  • Human--rat muscle somatic cell hybrids form myotubes and express human muscle gene products.

    abstract::Somatic cell hybrids have been prepared at high frequency between the rat muscle cell line L6 and human fetal muscle cells. The hybrid cells express several human gene products including an antigen, 12E7, controlled by the human X chromosome, Thy-1, and several human isoenzymes. In addition, one clone (37-11) expresse...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.8.5031

    authors: Quinn CA,Goodfellow PN,Povey S,Walsh FS

    更新日期:1981-08-01 00:00:00

  • Enkephalin-containing amacrine cells in the avian retina: immunohistochemical localization.

    abstract::The distribution of enkephalin-like immunoreactivity within the avian retina was studied by immunofluorescence and immunoperoxidase techniques with antiserum to [Met5]enkephalin and [Leu5]enkephalin. Formaldehyde-fixed retinae were sectioned and incubated in antiserum to either [Met5]- or [Leu5]enkephalin. Specificity...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.6.3010

    authors: Brecha N,Karten HJ,Laverack C

    更新日期:1979-06-01 00:00:00

  • Successful rescue of microsurgically produced homozygous uniparental mouse embryos via production of aggregation chimeras.

    abstract::Homozygous uniparental mouse embryos, produced by microsurgical removal of the male pronucleus from fertilized eggs and diploidization of the female pronucleus with cytochalasin, were surrounded with blastomeres from normal embryos to produce chimeric embryos. A few of these chimeras developed into viable adults, and ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.17.6509

    authors: Anderegg C,Markert CL

    更新日期:1986-09-01 00:00:00

  • Configurational diffusion down a folding funnel describes the dynamics of DNA hairpins.

    abstract::Elucidating the mechanism of folding of polynucleotides depends on accurate estimates of free energy surfaces and a quantitative description of the kinetics of structure formation. Here, the kinetics of hairpin formation in single-stranded DNA are measured after a laser temperature jump. The kinetics are modeled as co...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.131477798

    authors: Ansari A,Kuznetsov SV,Shen Y

    更新日期:2001-07-03 00:00:00

  • Raman resonance of electron donor/acceptor complex.

    abstract::The resonance Raman excitation profiles of a number of charge transfer transitions in electron donor/acceptor complexes with tetracyanoethylene as acceptor in solution at room temperature are reported and compared with absorption and fluorescence spectra of these complexes. All complexes show distinct anomalies which ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.72.11.4196

    authors: Michaelian KH,Rieckhoff KE,Voigt EM

    更新日期:1975-11-01 00:00:00