Characterization of RNA from noninfectious virions produced by sarcoma positive-leukemia negative transformed 3T3 cells.

abstract：:The unicellular green alga Chlamydomonas swims with two flagella that can synchronize their beat. Synchronized beating is required to swim both fast and straight. A long-standing hypothesis proposes that synchronization of flagella results from hydrodynamic coupling, but the details are not understood. Here, we presen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geyer VF,Jülicher F,Howard J,Friedrich BM

更新日期：2013-11-05 00:00:00

abstract：:The molecular mechanism of tolerance to opiate drugs is poorly understood. We have used single-channel patch-clamp recordings to study opiate receptor effects on dissociated neurons from rat amygdala, a limbic region implicated in addiction processes. A 130-pS inwardly rectifying K(+)-preferring cation channel was act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Marrero HG,Murphy R,Lin YJ,Freedman JE

更新日期：2000-12-19 00:00:00

abstract：:Eukaryotic cells respond to a variety of DNA insults by triggering a common signal transduction cascade, known as checkpoint response, which temporarily halts cell-cycle progression. Although the main players involved in the cascade have been identified, there is still uncertainty about the nature of the structures th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marini F,Nardo T,Giannattasio M,Minuzzo M,Stefanini M,Plevani P,Muzi Falconi M

更新日期：2006-11-14 00:00:00

abstract：:We have examined the functional consequences of mutations present in defective alleles of the retinoblastoma susceptibility gene (RB1) isolated from two spontaneously arising tumors. Unlike cDNA clones expressing the wild-type protein p110Rb, those encoding the two mutant proteins failed to induce the appearance of se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Templeton DJ,Park SH,Lanier L,Weinberg RA

更新日期：1991-04-15 00:00:00

abstract：:Enhancing immune responses with immune-modulatory monoclonal antibodies directed to inhibitory immune receptors is a promising modality in cancer therapy. Clinical efficacy has been demonstrated with antibodies blocking inhibitory immune checkpoints such as cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) or PD-1/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Romano E,Kusio-Kobialka M,Foukas PG,Baumgaertner P,Meyer C,Ballabeni P,Michielin O,Weide B,Romero P,Speiser DE

更新日期：2015-05-12 00:00:00

abstract：:The enzymes responsible for the oxidation of n-octane to octanoic acid or beyond in Pseudomonas oleovorans are octane inducible and are coded by genes borne on a transmissible extrachromosomal element. The octane to octanoate enzymes induced by octane are repressed by octanol. The chromosome also carries genes coding ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chakrabarty AM,Chou G,Gunsalus IC

更新日期：1973-04-01 00:00:00

abstract：:Individual cell types are defined by architecturally and functionally specialized cortical domains. The Ezrin, Radixin, and Moesin (ERM) proteins play a major role in organizing cortical domains by assembling membrane protein complexes and linking them to the cortical actin cytoskeleton. Many studies have focused on t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Casaletto JB,Saotome I,Curto M,McClatchey AI

更新日期：2011-07-19 00:00:00

abstract：:Flowering time (i.e., heading date in crops) is an important ecological trait that determines growing seasons and regional adaptability of plants to specific natural environments. Rice (Oryza sativa L.) is a short-day plant that originated in the tropics. Increasing evidence suggests that the northward expansion of cu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu W,Zheng XM,Lu G,Zhong Z,Gao H,Chen L,Wu C,Wang HJ,Wang Q,Zhou K,Wang JL,Wu F,Zhang X,Guo X,Cheng Z,Lei C,Lin Q,Jiang L,Wang H,Ge S,Wan J

更新日期：2013-02-19 00:00:00

abstract：:The amino acid sequence of the light chain of bovine protein C was determined by sequenator analysis of the carboxymethylated light chain and fragments obtained by cyanogen bromide treatment, tryptic digestion after blocking of lysine residues, and cleavage with 2-(2-nitrophenylsulfenyl)-3-methyl-3-bromoindolenine (BN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernlund P,Stenflo J,Tufvesson A

更新日期：1978-12-01 00:00:00

abstract：:We have recently shown that the Alzheimer disease 40-residue amyloid beta-protein [A beta P-(1-40)] can form cation-selective channels when incorporated into planar lipid bilayers by fusion of liposomes containing the peptide. Since A beta P-(1-40) comprises portions of the putative extracellular and membrane-spanning...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arispe N,Pollard HB,Rojas E

更新日期：1993-11-15 00:00:00

abstract：:The substrate specificity of thermophilic xylose isomerase from Clostridium thermosulfurogenes was examined by using predictions from the known crystal structure of the Arthrobacter enzyme and site-directed mutagenesis of the thermophile xylA gene. The orientation of glucose as a substrate in the active site of the th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meng M,Lee C,Bagdasarian M,Zeikus JG

更新日期：1991-05-01 00:00:00

abstract：:Duchenne muscular dystrophy (DMD) is a lethal genetic disorder caused by an absence of the dystrophin protein in bodywide muscles, including the heart. Cardiomyopathy is a leading cause of death in DMD. Exon skipping via synthetic phosphorodiamidate morpholino oligomers (PMOs) represents one of the most promising ther...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Echigoya Y,Nakamura A,Nagata T,Urasawa N,Lim KRQ,Trieu N,Panesar D,Kuraoka M,Moulton HM,Saito T,Aoki Y,Iversen P,Sazani P,Kole R,Maruyama R,Partridge T,Takeda S,Yokota T

更新日期：2017-04-18 00:00:00

abstract：:Compensatory ventilatory responses to increased inspiratory loading are essential for adequate breathing regulation in a number of pulmonary diseases; however, the human brain sites mediating such responses are unknown. Midsagittal and axial images were acquired in 11 healthy volunteers during unloaded and loaded (30 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gozal D,Omidvar O,Kirlew KA,Hathout GM,Hamilton R,Lufkin RB,Harper RM

更新日期：1995-07-03 00:00:00

abstract：:Nicotine exposure diminishes the protective breathing and arousal responses to stress (hypoxia). By exacerbating sleep-disordered breathing, this disturbance could underpin the well established association between smoking and the increased risk of sudden infant death syndrome. We show here that the protective response...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cohen G,Han ZY,Grailhe R,Gallego J,Gaultier C,Changeux JP,Lagercrantz H

更新日期：2002-10-01 00:00:00

abstract：:The defining feature of the mycobacterial outer membrane (OM) is the presence of mycolic acids (MAs), which, in part, render the bilayer extremely hydrophobic and impermeable to external insults, including many antibiotics. Although the biosynthetic pathway of MAs is well studied, the mechanism(s) by which these lipid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu Z,Meshcheryakov VA,Poce G,Chng SS

更新日期：2017-07-25 00:00:00

abstract：:The fluorescence of the supposedly buried tryptophan in ribonuclease T1 has been found to be collisionally quenched by acrylamide with a rate constant of 3 X 10(8) M--1 sec--1. Only a slight decrease in the quenching rate is observed upon a 5-fold increase in the viscosity of the solution. For this to be the case, the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eftink MR,Ghiron CA

更新日期：1975-09-01 00:00:00

abstract：:Memory is stored in neural networks via changes in synaptic strength mediated in part by NMDA receptor (NMDAR)-dependent long-term potentiation (LTP). Here we show that a cholecystokinin (CCK)-B receptor (CCKBR) antagonist blocks high-frequency stimulation-induced neocortical LTP, whereas local infusion of CCK induces...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Li X,Wong YT,Zheng X,Wang H,Peng Y,Feng H,Feng J,Baibado JT,Jesky R,Wang Z,Xie H,Sun W,Zhang Z,Zhang X,He L,Zhang N,Zhang Z,Tang P,Su J,Hu LL,Liu Q,He X,Tan A,Sun X,Li M,Wong K,Wang X,Cheung

更新日期：2019-03-26 00:00:00

abstract：:Cell migration and spreading involve the coordination of membrane trafficking, actomyosin contraction, and modifications to plasma membrane tension and area. The biochemical or biophysical basis for this coordination is however unknown. In this study, we show that during cell spreading, lamellipodia protrusion flatten...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gauthier NC,Fardin MA,Roca-Cusachs P,Sheetz MP

更新日期：2011-08-30 00:00:00

abstract：:AB-type toxins, like other bacterial toxins, are notably opportunistic molecules. They rely on target cell receptors to reach the appropriate location within the target cell where translocation of their enzymatic subunits occurs. The anthrax toxin, however, times its own uptake, suggesting that toxin binding triggers ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abrami L,Kunz B,van der Goot FG

更新日期：2010-01-26 00:00:00

abstract：:Selective therapeutics for nuclear receptors would revolutionize treatment for endocrine disease. Specific control of nuclear receptor activity is challenging because the internal cavities that bind hormones can be virtually identical. Only one highly selective hormone analog is known for the thyroid receptor, GC-24, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Borngraeber S,Budny MJ,Chiellini G,Cunha-Lima ST,Togashi M,Webb P,Baxter JD,Scanlan TS,Fletterick RJ

更新日期：2003-12-23 00:00:00

abstract：:Incubation of a human beta-globin pre-mRNA in a HeLa cell nuclear extract under conditions permissive for efficient splicing resulted in the assembly of the RNA into ribonucleoprotein (RNP) complexes. This RNP formation occurred largely within the characteristic lag period that precedes splicing. Two classes of RNP we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mayrand SH,Pedersen N,Pederson T

更新日期：1986-06-01 00:00:00

abstract：:Knowledge of normal human cardiac excitation stems from isolated heart or intraoperative mapping studies under nonphysiological conditions. Here, we use a noninvasive imaging modality (electrocardiographic imaging) to study normal activation and repolarization in intact unanesthetized healthy adults under complete phy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramanathan C,Jia P,Ghanem R,Ryu K,Rudy Y

更新日期：2006-04-18 00:00:00

abstract：:The molecular chaperone Hsp90 is a protein folding machine that is conserved from bacteria to man. Human, cytosolic Hsp90 is dedicated to folding of chiefly signal transduction components. The chaperoning mechanism of Hsp90 is controlled by ATP and various cochaperones, but is poorly understood and controversial. Here...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karagöz GE,Duarte AM,Ippel H,Uetrecht C,Sinnige T,van Rosmalen M,Hausmann J,Heck AJ,Boelens R,Rüdiger SG

更新日期：2011-01-11 00:00:00

abstract：:A cDNA, cRKIN1, encoding a putative homologue of the yeast (Saccharomyces cerevisiae) SNF1-encoded protein-serine/threonine kinase, has been isolated from a library prepared from rye endosperm mRNA. Northern blot analysis demonstrated the presence of cRKIN1-related transcripts in developing endosperms but not in shoot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alderson A,Sabelli PA,Dickinson JR,Cole D,Richardson M,Kreis M,Shewry PR,Halford NG

更新日期：1991-10-01 00:00:00

abstract：:The classic anthropological hypothesis known as the "obstetrical dilemma" is a well-known explanation for human altriciality, a condition that has significant implications for human social and behavioral evolution. The hypothesis holds that antagonistic selection for a large neonatal brain and a narrow, bipedal-adapte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dunsworth HM,Warrener AG,Deacon T,Ellison PT,Pontzer H

更新日期：2012-09-18 00:00:00

abstract：:Two independently arising alpha heavy chain mutants have been found to synthesize heavy chains with CH1 deletions of approximately equal extent. Both were isolated from heavy chain-producing variants of the mouse myeloma W3129 and demonstrate that it is possible to arrive at the heavy chain disease phenotype by the pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dackowski W,Morrison SL

更新日期：1981-11-01 00:00:00

abstract：:Biotin carboxylase [biotin-carboxyl-carrier-protein:carbon-dioxide ligase (ADP-forming), EC 6.3.4.14] is the enzyme mediating the first step of the acetyl-CoA carboxylase [acetyl-CoA:carbon-dioxide ligase (ADP-forming), EC 6.4.1.2] reaction. We screened an Escherichia coli DNA library and a DNA fragment carrying the b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kondo H,Shiratsuchi K,Yoshimoto T,Masuda T,Kitazono A,Tsuru D,Anai M,Sekiguchi M,Tanabe T

更新日期：1991-11-01 00:00:00

abstract：:Cytological preparations of interphase nuclei and chromosomes from mouse 3T6 cells prepared at various times after infection with the murine sarcomaleukemia virus complex were hybridized with the [(3)H]DNA product of the viral RNA-directed DNA polymerase. While uninfected nuclei had an average of 4 autoradiographic gr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loni MC,Green M

更新日期：1974-09-01 00:00:00

abstract：:Pathogen exit is a key stage in the spread and propagation of infectious disease, with the fecal-oral route being a common mode of disease transmission. However, it is poorly understood which molecular pathways provide the major modes for intracellular pathogen exit and fecal-oral transmission in vivo. Here, we use th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Szumowski SC,Botts MR,Popovich JJ,Smelkinson MG,Troemel ER

更新日期：2014-06-03 00:00:00

abstract：:Acute glaucoma is a sight-threatening condition characterized by a sudden and substantial rise in intraocular pressure (IOP) and consequent retinal ganglion cell (RGC) death. Angle closure glaucoma, a common cause of glaucoma in Asia that affects tens of millions of people worldwide, often presents acutely with loss o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chi W,Li F,Chen H,Wang Y,Zhu Y,Yang X,Zhu J,Wu F,Ouyang H,Ge J,Weinreb RN,Zhang K,Zhuo Y

更新日期：2014-07-29 00:00:00