Detection and localization of virus-specific DNA by in situ hybridization of cells during infection and rapid transformation by the murine sarcoma-leukemia virus.

abstract：:Genetic manipulation of the alpha(2A)-adrenergic receptor (alpha(2A)-AR) in mice has revealed the role of this subtype in numerous responses, including agonist-induced hypotension and sedation. Unexpectedly, alpha(2)-agonist treatment of mice heterozygous for the alpha(2A)-AR (alpha(2A)-AR(+/-)) lowers blood pressure ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tan CM,Wilson MH,MacMillan LB,Kobilka BK,Limbird LE

更新日期：2002-09-17 00:00:00

abstract：:The antagonists of gamma-aminobutyric acid, bicuculline and picrotoxin, were found to narrow the receptive field profile of the large field horizontal cell (L1HC) in the turtle retina when added to the perfusion medium in micromolar concentrations. The coupling resistance between neighboring L1HCs was increased by bic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Piccolino M,Neyton J,Witkovsky P,Gerschenfeld HM

更新日期：1982-06-01 00:00:00

abstract：:A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. Th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rossi JM,Burke DT,Leung JC,Koos DS,Chen H,Tilghman SM

更新日期：1992-03-15 00:00:00

abstract：:Cathepsin B (CTSB) is overexpressed in tumors of the lung, prostate, colon, breast, and stomach. However, evidence of primary genomic alterations in the CTSB gene during tumor initiation or progression has been lacking. We have found a novel amplicon at 8p22-23 that results in CTSB overexpression in esophageal adenoca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes SJ,Glover TW,Zhu XX,Kuick R,Thoraval D,Orringer MB,Beer DG,Hanash S

更新日期：1998-10-13 00:00:00

abstract：:The sources of human germline mutations are poorly understood. Part of the difficulty is that mutations occur very rarely, and so direct pedigree-based approaches remain limited in the numbers that they can examine. To address this problem, we consider the spectrum of low-frequency variants in a dataset (Genome Aggreg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agarwal I,Przeworski M

更新日期：2019-09-03 00:00:00

abstract：:Nuclear import of classical nuclear localization sequence-bearing proteins is mediated by karyopherin alpha/beta1 heterodimers. A second nuclear import pathway, mediated by karyopherin beta2 (transportin), recently was described for mRNA-binding proteins. Here we report the cloning and characterization of human karyop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yaseen NR,Blobel G

更新日期：1997-04-29 00:00:00

abstract：:Dematerialization, represented by declining consumption per GDP of energy or of goods, offers some hope for rising environmental quality with development. The declining proportion of income spent on staples as affluence grows, which income elasticity <1.0 measures, makes dematerialization widespread. Further, as learn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ausubel JH,Waggoner PE

更新日期：2008-09-02 00:00:00

abstract：:With efforts underway to develop a preventive human immunodeficiency virus type 1 (HIV-1) vaccine, it remains unclear which immune responses are sufficient to protect against infection and whether prior HIV-1 immunity can alter the subsequent course of HIV-1 infection. We investigated these issues in the context of a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McElrath MJ,Corey L,Greenberg PD,Matthews TJ,Montefiori DC,Rowen L,Hood L,Mullins JI

更新日期：1996-04-30 00:00:00

abstract：:The translational apparatus in cell-free extracts prepared from L cells infected with reovirus undergoes a time-dependent transition from cap dependence to cap independence. Extracts from uninfected L cells translate capped reovirus mRNA at high efficiency and synthesize the expected three size classes of reovirus pol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skup D,Millward S

更新日期：1980-01-01 00:00:00

abstract：:The amyloid beta-protein precursor (AbetaPP) is best known as the parent molecule to the amyloid beta-peptide that accumulates in the brains of patients with Alzheimer's disease. Secreted isoforms of AbetaPP that contain the Kunitz proteinase inhibitor domain are analogous to the previously identified cell-secreted pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu F,Davis J,Miao J,Previti ML,Romanov G,Ziegler K,Van Nostrand WE

更新日期：2005-12-13 00:00:00

abstract：:The recognition of carrier proteins by multiple catalytic partners occurs in every cycle of chain elongation in the biosynthesis of fatty acids and of the pharmacologically important polyketide and nonribosomal peptide natural products. To dissect the features of carrier proteins that determine specific recognition at...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou Z,Lai JR,Walsh CT

更新日期：2007-07-10 00:00:00

abstract：:Proteins need to be tightly regulated as they control biological processes in most normal cellular functions. The precise mechanisms of regulation are rarely completely understood but can involve binding of endogenous ligands and/or partner proteins at specific locations on a protein that can modulate function. Often,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ludlow RF,Verdonk ML,Saini HK,Tickle IJ,Jhoti H

更新日期：2015-12-29 00:00:00

abstract：:The broad negative consequences of habitat degradation on biodiversity have been studied, but the complex effects of natural-agricultural landscape matrices remain poorly understood. Here we used stable carbon and nitrogen isotopes to detect changes in mammal resource and habitat use and trophic structure between pres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Magioli M,Moreira MZ,Fonseca RCB,Ribeiro MC,Rodrigues MG,Ferraz KMPMB

更新日期：2019-09-10 00:00:00

abstract：:Lines containing two third-chromosome inversions (Arrowhead and Pikes Peak) of Drosophila pseudoobscura selected for fast and slow mating speed were crossed to a multichromosomal, dominant marker inversion stock, and strains with various combinations of chromosomal derivation were tested for their mating activity. Whe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sherwin RN,Spiess EB

更新日期：1973-02-01 00:00:00

abstract：:We have found that poly(L-lysine) can be a very effective agent in preventing the growth of Ehrlich ascites tumors in mice. When given optimal doses of poly(L-lysine) (Mr 60 x 10(3)) intraperitoneally for 5 consecutive days, beginning on day 1 after inoculation with Ehrlich ascites cells. White Swiss mice show nearly ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arnold LJ Jr,Dagan A,Gutheil J,Kaplan NO

更新日期：1979-07-01 00:00:00

abstract：:The microvillus core-filament bundle from intestinal epithelial cells is a highly ordered structure containing actin and four major associated proteins. Two of these, villin and calmodulin, bind calcium ions (Kd approximately 10(-6) M) in the physiologically important range. Because ethylene glycol bis(beta-aminoethyl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glenney JR Jr,Bretscher A,Weber K

更新日期：1980-11-01 00:00:00

abstract：:Most evidence supports the view that ER alpha is responsible for estrogen (ovarian estradiol, E(2))-induced proliferation in the epithelial cells of the mammary gland, but despite this, proliferating epithelial cells do not express ER alpha. We have examined this apparent paradox by studying the role of ER alpha and E...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng G,Weihua Z,Warner M,Gustafsson JA

更新日期：2004-03-16 00:00:00

abstract：:Molecular analyses of Na,K-ATPase abundance and alpha-subunit isoform distribution were performed to determine whether pump expression varies at different points through the thickness of the circular layer of colonic smooth muscle. The mRNA and polypeptides of Na,K-ATPase alpha 1 and beta subunits were twice as abunda...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burke EP,Sanders KM,Horowitz B

更新日期：1991-03-15 00:00:00

abstract：:The endbrain (telencephalon) is at the rostral end of the central nervous system and is primarily responsible for supporting cognition and affect. Structurally, it consists of right and left cerebral hemispheres, each parceled into multiple cortical and nuclear gray matter regions. The global network organization of a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swanson LW,Hahn JD,Jeub LGS,Fortunato S,Sporns O

更新日期：2018-07-17 00:00:00

abstract：:BALB/c mice immunized with syngeneic methylcholanthrene-induced fibrosarcomas did not have antibodies against the unique tumor-specific transplantation antigens, even though they were capable of rejecting a lethal dose of tumor cells. Endogenous murine leukemia virus antigens expressed by certain of the tumors did, ho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown JP,Klitzman JM,Hellström I,Nowinski RC,Hellström KE

更新日期：1978-02-01 00:00:00

abstract：:Heterotrimeric G proteins relay extracellular cues from heptahelical transmembrane receptors to downstream effector molecules. Composed of an alpha subunit with intrinsic GTPase activity and a betagamma heterodimer, the trimeric complex dissociates upon receptor-mediated nucleotide exchange on the alpha subunit, enabl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Slep KC,Kercher MA,Wieland T,Chen CK,Simon MI,Sigler PB

更新日期：2008-04-29 00:00:00

abstract：:Cell cultures of Catharanthus roseus were supplied with [2-13C, 3-2H]-deoxyxylulose or [2-13C,4-2H]1-deoxyxylulose. Lutein and chlorophylls were isolated from the cell mass, and hydrolysis of the chlorophyll mixtures afforded phytol. Isotope labeling patterns of phytol and lutein were determined by 2H NMR and 1H,2H-de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arigoni D,Eisenreich W,Latzel C,Sagner S,Radykewicz T,Zenk MH,Bacher A

更新日期：1999-02-16 00:00:00

abstract：:Blood cells transplantation is largely replacing bone marrow transplantation because engraftment is more rapid. This accelerated engraftment is thought to be mediated by relatively mature committed hematopoietic progenitor cells. Herein, we have used a modified rhodamine (Rho) staining procedure to identify and purify...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zijlmans JM,Visser JW,Laterveer L,Kleiverda K,Heemskerk DP,Kluin PM,Willemze R,Fibbe WE

更新日期：1998-01-20 00:00:00

abstract：:MicroRNAs are predicted to regulate approximately 30% of all human genes by targeting sequences in their 3' UTR. Polymorphisms in 3' UTR of several genes have been reported to affect gene expression, but the mechanism is not fully understood. Here, we demonstrate that 829C-->T, a naturally occurring SNP, near the miR-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mishra PJ,Humeniuk R,Mishra PJ,Longo-Sorbello GS,Banerjee D,Bertino JR

更新日期：2007-08-14 00:00:00

abstract：:The hemoglobin oxidation Bohr effect below pH 7 is essentially proportional to the fraction of hemes oxidized, just as the ligation Bohr effect is proportional to fractional heme ligation. The reported nonlinear proton release during oxidation [(1965) J. Biol. Chem. 240, 3317-3324] is shown to be an artifact resulting...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoffman BM,Bull C

更新日期：1976-03-01 00:00:00

abstract：:Volume changes associated with protein folding reactions contain valuable information about the folding mechanism and the nature of the transition state. However, meaningful interpretation of such data requires that overall volume changes be deconvoluted into individual contributions from different structural componen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neumaier S,Büttner M,Bachmann A,Kiefhaber T

更新日期：2013-12-24 00:00:00

abstract：:We have found that three Escherichia coli heat shock proteins, DnaK (the hsp70 homolog), DnaJ, and GrpE, function in oriP1 DNA replication in vitro solely to activate DNA binding by the replication initiator protein RepA. Activation results from the conversion of P1 or P7 RepA dimers to monomers that bind with high af...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wickner S,Skowyra D,Hoskins J,McKenney K

更新日期：1992-11-01 00:00:00

abstract：:The prohibitin (PHB)-domain proteins are membrane proteins that regulate a variety of biological activities, including mechanosensation, osmotic homeostasis, and cell signaling, although the mechanism of this regulation is unknown. We have studied two members of this large protein family, MEC-2, which is needed for to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huber TB,Schermer B,Müller RU,Höhne M,Bartram M,Calixto A,Hagmann H,Reinhardt C,Koos F,Kunzelmann K,Shirokova E,Krautwurst D,Harteneck C,Simons M,Pavenstädt H,Kerjaschki D,Thiele C,Walz G,Chalfie M,Benzing T

更新日期：2006-11-14 00:00:00

abstract：:RS67333 is a partial serotonin subtype 4 receptor (5-HT4R) agonist that has been widely studied for its procognitive effect. More recently, it has been shown that its ability to promote the nonamyloidogenic cleavage of the precursor of the neurotoxic amyloid-β peptide leads to the secretion of the neurotrophic protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lecoutey C,Hedou D,Freret T,Giannoni P,Gaven F,Since M,Bouet V,Ballandonne C,Corvaisier S,Malzert Fréon A,Mignani S,Cresteil T,Boulouard M,Claeysen S,Rochais C,Dallemagne P

更新日期：2014-09-09 00:00:00

abstract：:Kinetic analysis of the in vitro reconstitution of glyceraldehyde-3-phosphate dehydrogenase [D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating), EC 1.2.1.12] from yeast showed that both oxidized and reduced coenzyme enhance the transconformation reaction, which is rate limiting in the sequential foldin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jaenicke R,Krebs H,Rudolph R,Woenckhaus C

更新日期：1980-04-01 00:00:00