当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Rate enhancement of reconstitution of glyceraldehyde-3-phosphate dehydrogenase by a covalently bound coenzyme analog.

Rate enhancement of reconstitution of glyceraldehyde-3-phosphate dehydrogenase by a covalently bound coenzyme analog.

Abstract：

:Kinetic analysis of the in vitro reconstitution of glyceraldehyde-3-phosphate dehydrogenase [D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating), EC 1.2.1.12] from yeast showed that both oxidized and reduced coenzyme enhance the transconformation reaction, which is rate limiting in the sequential folding-association process at high enzyme concentrations (Krebs, H., Rudolph, R. & Jaenicke, R. (1979) Eur. J. Biochem. 100, 359-364). In the present study the reconstitution of the enzyme has been analyzed after covalent modification with the coenzyme analog 3-[(3-bromoacetylpyridinio)-propyl]adenosine pyrophosphate. Reconstitution of the modified enzyme, as determined by the regain of the native tryptophan fluorescence, is found to be more then 10 times faster than refolding of the unmodified apoenzyme and more than 5 times faster than that of the unmodified holoenzyme. Various degrees of denaturation and the presence of up to 0.4 M guanidine . HCl do not affect the rate of reconstitution of the modified enzyme. The kinetic effect of free or covalently bound coenzyme is discussed in terms of a decrease in free energy of the native or native-like structure or in terms of a decreased activation energy of rate-limiting steps in the process of reconstitution. Stabilization of the dimeric intermediate or acceleration of its transformation seems to be the most likely explanation for the observed effect of free or covalently bound coenzyme on the rate of reconstitution.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Jaenicke R,Krebs H,Rudolph R,Woenckhaus C

doi

10.1073/pnas.77.4.1966

subject

Has Abstract

pub_date

1980-04-01 00:00:00

pages

1966-9

issue

4

eissn

0027-8424

issn

1091-6490

journal_volume

77

pub_type

杂志文章

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