Abstract:
:Host species have evolved mechanisms that can inhibit pathogen replication even after a cell has been successfully invaded. Here we show that tripartite-motif protein 21 (TRIM21), a ubiquitously expressed E3 ubiquitin ligase that targets viruses inside the cytosol, protects mice against fatal viral infection. Upon infection with mouse adenovirus-1, naive mice lacking TRIM21 succumb to encephalomyelitis within 7 d. In contrast, wild-type mice rapidly up-regulate TRIM21 and control viremia. Trim21 heterozygous mice have a haploinsufficiency phenotype in which reduced TRIM21 expression leads to a viral load that is higher than wild types but lower than knockouts. TRIM21 is a high-affinity antibody receptor that allows antibodies to operate inside an infected cell. In passive transfer experiments at high viral dose, antisera that fully protects wild-type mice fails to protect most Trim21 knockout animals. These results demonstrate that TRIM21 provides potent antiviral protection and forms an important part of the humoral immune response.
journal_name
Proc Natl Acad Sci U S Aauthors
Vaysburd M,Watkinson RE,Cooper H,Reed M,O'Connell K,Smith J,Cruickshanks J,James LCdoi
10.1073/pnas.1301918110subject
Has Abstractpub_date
2013-07-23 00:00:00pages
12397-401issue
30eissn
0027-8424issn
1091-6490pii
1301918110journal_volume
110pub_type
杂志文章abstract::The rates of reassociation of DNA from a monkey cell line (Vero), from SV40 pseudovirus (consisting of Vero DNA and virus protein coat), and from mature SV40 were measured. The results show that the host DNA in the pseudovirus contains repeated and unique sequences in the same proportions as normal host DNA; hence, no...
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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