Engineering precision RNA molecular switches.

abstract：:The S-M checkpoint delays mitosis until DNA replication is complete; cells defective in this checkpoint lose viability when DNA replication is inhibited. This inviability can be suppressed in fission yeast by overexpression of Cid1 or the related protein Cid13. Fission yeast contain six cid1/cid13-like genes, whereas ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Read RL,Martinho RG,Wang SW,Carr AM,Norbury CJ

更新日期：2002-09-17 00:00:00

abstract：:Allostery, the coupling between ligand binding and protein conformational change, is the heart of biological network and it has often been explained by two representative models, the induced-fit and the population-shift models. Here, we clarified for what systems one model fits better than the other by performing mole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okazaki K,Takada S

更新日期：2008-08-12 00:00:00

abstract：:Maturation of Asn-linked oligosaccharides in the eukaryotic secretory pathway requires the trimming of nascent glycan chains to remove all glucose and several mannose residues before extension into complex-type structures on the cell surface and secreted glycoproteins. Multiple glycoside hydrolase family 47 (GH47) α-m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiang Y,Karaveg K,Moremen KW

更新日期：2016-12-06 00:00:00

abstract：:We use the inverse scattering method to determine the weak limit of solutions of the Korteweg-deVries equation as dispersion tends to zero. The limit, valid for all time, is characterized in terms of a quadratic programming problem which can be solved with the aid of function theoretic methods. For large t, the soluti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lax PD,Levermore CD

更新日期：1979-08-01 00:00:00

abstract：:Homeobox genes constitute a large family of transcription factors that are essential during normal development and are often dysregulated in cancer. However, the molecular mechanisms by which homeobox genes influence cancer remain largely unknown. Here we show that the tissue-restricted cyclin A1 is a transcriptional ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coletta RD,Christensen K,Reichenberger KJ,Lamb J,Micomonaco D,Huang L,Wolf DM,Müller-Tidow C,Golub TR,Kawakami K,Ford HL

更新日期：2004-04-27 00:00:00

abstract：:The aldosterone-induced augmentation of Na+ transport in toad bladder was analyzed by comparing the hormonal actions on the transepithelial short-circuit current and on the amiloride-sensitive 22Na+ uptake in isolated membrane vesicles. Incubating bladders with 0.5 microM aldosterone for 3 hr evoked more than a 2-fold...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Asher C,Garty H

更新日期：1988-10-01 00:00:00

abstract：:In organisms possessing a dispersal phase the processes of mating, competition, feeding, and predation are often carried out within "trait-groups," defined as populations enclosed in areas smaller than the boundaries of the deme. A simple model shows that this can lead to the selection of "altruistic" traits that favo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wilson DS

更新日期：1975-01-01 00:00:00

abstract：:Normal as well as Rous sarcoma virus-infected chicken pectoral and chicken embryo fibroblasts proliferate actively in a plasma containing medium of physiological ion concentrations (Ca2+, 1.2 mM; Mg2+, 0.7 mM). Reduction of medium calcium and magnesium concentrations is necessary to achieve selective quiescence of nor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balk SD

更新日期：1980-11-01 00:00:00

abstract：:We provide empirical evidence of immigrants' specialization in skill acquisition well before entering the US labor market. Nationally representative datasets enable studying the academic trajectories of immigrant children, with a focus on high-school course-taking patterns and college major choice. Immigrant children ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rangel MA,Shi Y

更新日期：2019-01-08 00:00:00

abstract：:We have studied ten phenotypically similar patients with complete androgen insensitivity. All of the patients tested had significantly elevated serum luteinizing hormone and plasma androgens within or above the normal adult male range. On the basis of specific dihydrotestosterone binding by skin fibroblasts, we identi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amrhein JA,Meyer WJ 3rd,Jones HW Jr,Migeon CJ

更新日期：1976-03-01 00:00:00

abstract：:A shuttle vector plasmid that contains sequences from simian virus 40, pBR322, and a bacterial marker gene, galactokinase, has been constructed. After replication in cells permissive for virus progeny, plasmid DNA was introduced into a galactokinase-deficient bacterial strain and the relative frequency of colonies wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Razzaque A,Mizusawa H,Seidman MM

更新日期：1983-05-01 00:00:00

abstract：:The synthesis of a photosensitive blocking group for the carboxyl function of neurotransmitters, in this case glycine, is reported. The compound, 2-methoxy-5-nitrophenyl glycine ester (caged glycine), is photolyzed by a laser pulse at 308 or 337 nm within 3 microseconds and with a product quantum yield of 0.2. The com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramesh D,Wieboldt R,Niu L,Carpenter BK,Hess GP

更新日期：1993-12-01 00:00:00

abstract：:p53, the most commonly mutated gene in human tumors, is believed to play a crucial role in the prevention of cancer by protecting cells from mutation, a theory commonly known as the "Guardian of the Genome" hypothesis. There are two hypotheses as to how this can occur. In the first, p53 protects the genome by retardin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bielas JH,Heddle JA

更新日期：2003-10-28 00:00:00

abstract：:Our laboratory has previously developed cell lines derived from mouse NIH 3T3 fibroblasts and C127 mammary tumor cells that stably express mouse mammary tumor virus (MMTV) long terminal repeat fusion genes in bovine papillomavirus-based episomes. Glucocorticoid hormone strongly activates transcription from episomes an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bresnick EH,John S,Berard DS,LeFebvre P,Hager GL

更新日期：1990-05-01 00:00:00

abstract：:The localized maternal RNA Vg1 resides in the cortical region of the vegetal pole of fully grown Xenopus oocytes and is inherited by only a subset of blastomeres in the early embryo [Weeks, D. L. & Melton, D. A. (1987) Cell 51, 861-867]. Because RNA-cytoskeletal interactions may play a role in RNA localization, we hav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pondel MD,King ML

更新日期：1988-10-01 00:00:00

abstract：:Circadian dysfunction is a common attribute of many neurodegenerative diseases, most of which are associated with neuroinflammation. Circadian rhythm dysfunction has been associated with inflammation in the periphery, but the role of the core clock in neuroinflammation remains poorly understood. Here we demonstrate th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Griffin P,Dimitry JM,Sheehan PW,Lananna BV,Guo C,Robinette ML,Hayes ME,Cedeño MR,Nadarajah CJ,Ezerskiy LA,Colonna M,Zhang J,Bauer AQ,Burris TP,Musiek ES

更新日期：2019-03-12 00:00:00

abstract：:Satellite tobacco necrosis virus (STNV) is one of the smallest viruses known. Its genome encodes only its coat protein (CP) subunit, relying on the polymerase of its helper virus TNV for replication. The genome has been shown to contain a cryptic set of dispersed assembly signals in the form of stem-loops that each pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patel N,Wroblewski E,Leonov G,Phillips SEV,Tuma R,Twarock R,Stockley PG

更新日期：2017-11-14 00:00:00

abstract：:Chronic fatigue immune dysfunction syndrome (CFIDS) is a recently recognized illness characterized by debilitating fatigue as well as immunological and neurological abnormalities [Straus, S.E. (1988) J. Inf. Dis. 157, 405-412]. Once thought to be caused by Epstein-Barr virus, it is now thought to have a different but ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeFreitas E,Hilliard B,Cheney PR,Bell DS,Kiggundu E,Sankey D,Wroblewska Z,Palladino M,Woodward JP,Koprowski H

更新日期：1991-04-01 00:00:00

abstract：:Most small multicopy antibiotic-resistance plasmids of Staphylococcus aureus contain a major axis of hyphenated dyad symmetry (palA) that is required for normal replication and stability, although located outside of the minimal replicon. Rearrangements affecting palA cause plasmid instability, a marked reduction in co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gruss AD,Ross HF,Novick RP

更新日期：1987-04-01 00:00:00

abstract：:Oligodeoxyribonucleotides covalently linked to an intercalating agent via a polymethylene linker were synthesized. Oligothymidylates attached to an acridine dye (Acr) through the 3'-phosphate group [(Tp)n(CH2) mAcr ] specifically interact with the complementary sequence. The interaction is strongly stabilized by the i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Asseline U,Delarue M,Lancelot G,Toulmé F,Thuong NT,Montenay-Garestier T,Hélène C

更新日期：1984-06-01 00:00:00

abstract：:We analyzed variability in outer surface protein B (OspB) from Borrelia burgdorferi (Bb), the causative agent of Lyme disease, to determine how Bb escapes immune destruction. We have shown that vaccination with OspB from Bb strain B31 protected mice from infection with Bb B31 but not against Bb N40. The present study ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fikrig E,Tao H,Kantor FS,Barthold SW,Flavell RA

更新日期：1993-05-01 00:00:00

abstract：:In enzyme catalysis, where exquisitely positioned functionality is the sine qua non, atomic coordinates for a Michaelis complex can provide powerful insights into activation of the substrate. We focus here on the initial proton transfer of the isomerization reaction catalyzed by triosephosphate isomerase and present t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jogl G,Rozovsky S,McDermott AE,Tong L

更新日期：2003-01-07 00:00:00

abstract：:Disruption of neurotransmitter vesicle dynamics (transport, capacity, release) has been implicated in a variety of neurodegenerative and neuropsychiatric conditions. Here, we report a novel mouse model of enhanced vesicular function via bacterial artificial chromosome (BAC)-mediated overexpression of the vesicular mon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lohr KM,Bernstein AI,Stout KA,Dunn AR,Lazo CR,Alter SP,Wang M,Li Y,Fan X,Hess EJ,Yi H,Vecchio LM,Goldstein DS,Guillot TS,Salahpour A,Miller GW

更新日期：2014-07-08 00:00:00

abstract：:The day we understand the time evolution of subcellular events at a level of detail comparable to physical systems governed by Newton's laws of motion seems far away. Even so, quantitative approaches to cellular dynamics add to our understanding of cell biology. With data-guided frameworks we can develop better predic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ronquist S,Patterson G,Muir LA,Lindsly S,Chen H,Brown M,Wicha MS,Bloch A,Brockett R,Rajapakse I

更新日期：2017-11-07 00:00:00

abstract：:Two RNA fractions have been isolated and purified from both oncogenic and nononcogenic strains of Agrobacterium tumefaciens. Both RNAs are capable of inducing the formation of transplantable tumors when introduced at wound sites in stems of Datura stramonium plants. One of these RNA fractions was found to be bound to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beljanski M,Aaron-da Cunha MI,Beljanski MS,Manigault P,Bourgarel P

更新日期：1974-05-01 00:00:00

abstract：:Using GFP to mark recent thymic emigrants (RTEs) in mice carrying a GFP transgene driven by the recombination-activating gene 2 promoter, we demonstrate that RTEs are readily detectable even in 2-year-old mice, despite the fact that the proportion of the peripheral T cell pool comprised of RTEs declines with age. Alth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hale JS,Boursalian TE,Turk GL,Fink PJ

更新日期：2006-05-30 00:00:00

abstract：:Inhibition of human purine nucleoside phosphorylase (PNP) stops growth of activated T-cells and the formation of 6-oxypurine bases, making it a target for leukemia, autoimmune disorders, and gout. Four generations of ribocation transition-state mimics bound to PNP are structurally characterized. Immucillin-H (K*i(1/4)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ho MC,Shi W,Rinaldo-Matthis A,Tyler PC,Evans GB,Clinch K,Almo SC,Schramm VL

更新日期：2010-03-16 00:00:00

abstract：:Histone deacetylase inhibitors (HDACi) represent a new group of drugs currently being tested in a wide variety of clinical applications. They are especially effective in preclinical models of cancer where they show antiproliferative action in many different types of cancer cells. Recently, the first HDACi was approved...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haberland M,Johnson A,Mokalled MH,Montgomery RL,Olson EN

更新日期：2009-05-12 00:00:00

abstract：:The adzuki bean beetle, Callosobruchus chinensis, is triple-infected with distinct lineages of Wolbachia endosymbiont, wBruCon, wBruOri, and wBruAus, which were identified by their wsp (Wolbachia surface protein) gene sequences. Whereas wBruCon and wBruOri caused cytoplasmic incompatibility of the host insect, wBruAus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kondo N,Nikoh N,Ijichi N,Shimada M,Fukatsu T

更新日期：2002-10-29 00:00:00

abstract：:Metagenomics is expanding our knowledge of the gene content, functional significance, and genetic variability in natural microbial communities. Still, there exists limited information concerning the regulation and dynamics of genes in the environment. We report here global analysis of expressed genes in a naturally oc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frias-Lopez J,Shi Y,Tyson GW,Coleman ML,Schuster SC,Chisholm SW,Delong EF

更新日期：2008-03-11 00:00:00