当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Optimal alignment for enzymatic proton transfer: structure of the Michaelis complex of triosephosphate isomerase at 1.2-A resolution.

Optimal alignment for enzymatic proton transfer: structure of the Michaelis complex of triosephosphate isomerase at 1.2-A resolution.

Abstract：

:In enzyme catalysis, where exquisitely positioned functionality is the sine qua non, atomic coordinates for a Michaelis complex can provide powerful insights into activation of the substrate. We focus here on the initial proton transfer of the isomerization reaction catalyzed by triosephosphate isomerase and present the crystal structure of its Michaelis complex with the substrate dihydroxyacetone phosphate at near-atomic resolution. The active site is highly compact, with unusually short and bifurcated hydrogen bonds for both catalytic Glu-165 and His-95 residues. The carboxylate oxygen of the catalytic base Glu-165 is positioned in an unprecedented close interaction with the ketone and the alpha-hydroxy carbons of the substrate (C em leader O approximately 3.0 A), which is optimal for the proton transfer involving these centers. The electrophile that polarizes the substrate, His-95, has close contacts to the substrate's O1 and O2 (N em leader O < or = 3.0 and 2.6 A, respectively). The substrate is conformationally relaxed in the Michaelis complex: the phosphate group is out of the plane of the ketone group, and the hydroxy and ketone oxygen atoms are not in the cisoid configuration. The epsilon ammonium group of the electrophilic Lys-12 is within hydrogen-bonding distance of the substrate's ketone oxygen, the bridging oxygen, and a terminal phosphate's oxygen, suggesting a role for this residue in both catalysis and in controlling the flexibility of active-site loop.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Jogl G,Rozovsky S,McDermott AE,Tong L

doi

10.1073/pnas.0233793100

keywords:

subject

Has Abstract

pub_date

2003-01-07 00:00:00

pages

50-5

issue

1

eissn

0027-8424

issn

1091-6490

pii

0233793100

journal_volume

100

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Protease-independent action of tissue plasminogen activator in brain plasticity and neurological recovery after ischemic stroke.

    abstract：:Emerging evidence suggests that tissue plasminogen activator (tPA), currently the only FDA-approved medication for ischemic stroke, exerts important biological actions on the CNS besides its well-known thrombolytic effect. In this study, we investigated the role of tPA on primary neurons in culture and on brain recove...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1821979116

    authors： Pu H,Shi Y,Zhang L,Lu Z,Ye Q,Leak RK,Xu F,Ma S,Mu H,Wei Z,Xu N,Xia Y,Hu X,Hitchens TK,Bennett MVL,Chen J

    更新日期：2019-04-30 00:00:00

  • Full-length and truncated neurokinin-1 receptor expression and function during monocyte/macrophage differentiation.

    abstract：:The substance P (SP)-preferring receptor neurokinin-1 receptor (NK-1R) has two forms: a full-length receptor consisting of 407 aa and a truncated receptor consisting of 311 aa. These two receptors differ in the length of the C terminus of NK-1R. We studied the undifferentiated and phorbol myristate acetate (PMA)-diffe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0602563103

    authors： Lai JP,Ho WZ,Kilpatrick LE,Wang X,Tuluc F,Korchak HM,Douglas SD

    更新日期：2006-05-16 00:00:00

  • Differentiation of 3T3-L2 fibroblasts into adipose cells in bromodeoxyuridine-suppressed cultures.

    abstract：:Growth of 3T3-L2 fibroblasts in medium containing 5-bromo-2'-deoxyuridine prevents the spontaneous cytodifferentiation of these cells into adipose cells. Treatment of bromodeoxyuridine-suppressed cultures at confluence with 1-methyl-3-isobutylxanthine, a promoter of the adipose conversion, results in differentiation o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.9.4451

    authors： Russell TR

    更新日期：1979-09-01 00:00:00

  • Association of rat8 with Fyn protein kinase via lipid rafts is required for rat mammary cell differentiation in vitro.

    abstract：:We previously identified rat8 in the pathway involved in epithelial cell differentiation that occurs in the rat mammary gland at pregnancy when tubules and alveoli are formed. rat8, which encodes an IFN-inducible membrane protein, is the rat homologue of the mouse gene fragilis. By differential detergent extraction an...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0307292101

    authors： Zucchi I,Prinetti A,Scotti M,Valsecchi V,Valaperta R,Mento E,Reinbold R,Vezzoni P,Sonnino S,Albertini A,Dulbecco R

    更新日期：2004-02-17 00:00:00

  • Origin of the Metazoa.

    abstract：:The origin of the multicellular animals has been investigated by rate invariant analysis of 18S rRNA sequences. These analyses indicate that (i) the Metazoa is a monophyletic taxon; (ii) the Deuterostomia is a monophyletic taxon; (iii) the Annelida-Mollusca lineage is the sister group of an arthropod subgroup; and (iv...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.2.763

    authors： Lake JA

    更新日期：1990-01-01 00:00:00

  • Modification of histidine residues in proteins by reaction with 4-hydroxynonenal.

    abstract：:We find that histidine residues in proteins are major targets for reaction with the lipid peroxidation product 4-hydroxynon-2-enal (HNE). Reaction of insulin (which contains no sulfhydryl groups) with HNE leads to the generation of HNE-protein adducts, which are converted to radioactive derivatives upon subsequent tre...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.10.4544

    authors： Uchida K,Stadtman ER

    更新日期：1992-05-15 00:00:00

  • Recombinant human insulin-like growth factor I exerts a trophic action and confers glutamate sensitivity on glutamate-resistant cerebellar granule cells.

    abstract：:Cerebellar granule cells grown in the presence of a serum complex differentiate but are resistant to the lethal action of excitatory amino acids. When these cells are grown also in the presence of insulin-like growth factor I (IGF-I) they become fully susceptible to the toxic, lethal action of glutamate. The glutamate...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.18.8752

    authors： Calissano P,Ciotti MT,Battistini L,Zona C,Angelini A,Merlo D,Mercanti D

    更新日期：1993-09-15 00:00:00

  • Complete correction of murine Artemis immunodeficiency by lentiviral vector-mediated gene transfer.

    abstract：:Artemis gene mutations are responsible for the development of a severe combined immunodeficiency [radiation-sensitive (RS) SCID] characterized by a severe B and T cell deficiency and a normal natural killer cell population. To establish the feasibility of a gene therapy approach to the treatment of RS-SCID, we generat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0608130103

    authors： Mostoslavsky G,Fabian AJ,Rooney S,Alt FW,Mulligan RC

    更新日期：2006-10-31 00:00:00

  • Enhanced microtubule-dependent trafficking and p53 nuclear accumulation by suppression of microtubule dynamics.

    abstract：:The tumor suppressor protein p53 localizes to microtubules (MT) and, in response to DNA damage, is transported to the nucleus via the MT minus-end-directed motor protein dynein. Dynein is also responsible for MT-mediated nuclear targeting of adenovirus type 2 (Ad2). Here we show that treatment with low concentrations ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.132275599

    authors： Giannakakou P,Nakano M,Nicolaou KC,O'Brate A,Yu J,Blagosklonny MV,Greber UF,Fojo T

    更新日期：2002-08-06 00:00:00

  • The right incentives enable ocean sustainability successes and provide hope for the future.

    abstract：:Healthy ocean ecosystems are needed to sustain people and livelihoods and to achieve the United Nations Sustainable Development Goals. Using the ocean sustainably requires overcoming many formidable challenges: overfishing, climate change, ocean acidification, and pollution. Despite gloomy forecasts, there is reason f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1604982113

    authors： Lubchenco J,Cerny-Chipman EB,Reimer JN,Levin SA

    更新日期：2016-12-20 00:00:00

  • TARP modulation of synaptic AMPA receptor trafficking and gating depends on multiple intracellular domains.

    abstract：:Previous work has established stargazin and its related family of transmembrane AMPA receptor regulatory proteins (TARPs) as auxiliary subunits of AMPA receptors (AMPARs) that control synaptic strength both by targeting AMPARs to synapses through an intracellular PDZ-binding motif and by modulating their gating throug...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0905570106

    authors： Milstein AD,Nicoll RA

    更新日期：2009-07-07 00:00:00

  • Importance of newly generated neurons in the adult olfactory bulb for odor discrimination.

    abstract：:In adult rodents, neurons are continually generated in the subventricular zone of the forebrain, from where they migrate tangentially toward the olfactory bulb, the only known target for these neuronal precursors. Within the main olfactory bulb, they ascend radially into the granule and periglomerular cell layers, whe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.4.1823

    authors： Gheusi G,Cremer H,McLean H,Chazal G,Vincent JD,Lledo PM

    更新日期：2000-02-15 00:00:00

  • Seminaphthofluorones are a family of water-soluble, low molecular weight, NIR-emitting fluorophores.

    abstract：:A readily accessible new class of near infrared (NIR) molecular probes has been synthesized and evaluated. Specific fluorophores in this unique xanthene based regioisomeric seminaphthofluorone dye series exhibit a combination of desirable characteristics including (i) low molecular weight (339 amu), (ii) aqueous solub...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0710341105

    authors： Yang Y,Lowry M,Xu X,Escobedo JO,Sibrian-Vazquez M,Wong L,Schowalter CM,Jensen TJ,Fronczek FR,Warner IM,Strongin RM

    更新日期：2008-07-01 00:00:00

  • Long-distance correlations of rhinovirus capsid dynamics contribute to uncoating and antiviral activity.

    abstract：:Human rhinovirus (HRV) and other members of the enterovirus genus bind small-molecule antiviral compounds in a cavity buried within the viral capsid protein VP1. These compounds block the release of the viral protein VP4 and RNA from inside the capsid during the uncoating process. In addition, the antiviral compounds ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1119174109

    authors： Roy A,Post CB

    更新日期：2012-04-03 00:00:00

  • Metabolic engineering with Dof1 transcription factor in plants: Improved nitrogen assimilation and growth under low-nitrogen conditions.

    abstract：:Utilization of transcription factors might be a powerful approach to modification of metabolism for a generation of crops having superior characteristics because a single transcription factor frequently regulates coordinated expression of a set of key genes for respective pathways. Here, we apply the plant-specific Do...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0402267101

    authors： Yanagisawa S,Akiyama A,Kisaka H,Uchimiya H,Miwa T

    更新日期：2004-05-18 00:00:00

  • Rapid elimination of mature autoreactive B cells demonstrated by Cre-induced change in B cell antigen receptor specificity in vivo.

    abstract：:Developing autoreactive B cells edit their B cell antigen receptor (BCR) in the bone marrow and are clonally deleted when they fail to reexpress an innocent BCR. Here, inducible Cre-loxP-mediated gene inversion is used to change the specificity of the BCR on mature IgM+ IgD+ B cells in vivo to address the fate of lymp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.22.13171

    authors： Lam KP,Rajewsky K

    更新日期：1998-10-27 00:00:00

  • ERK regulation of phosphodiesterase 4 enhances dopamine-stimulated AMPA receptor membrane insertion.

    abstract：:AMPA-type glutamate receptor (AMPAR) trafficking is essential for modulating synaptic transmission strength. Prior studies that have characterized signaling pathways underlying AMPAR trafficking have identified the cAMP/PKA-mediated phosphorylation of GluA1, an AMPAR subunit, as a key step in the membrane insertion of...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1311783110

    authors： Song RS,Massenburg B,Wenderski W,Jayaraman V,Thompson L,Neves SR

    更新日期：2013-09-17 00:00:00

  • Chromosomal location targets different MYC family gene members for oncogenic translocations.

    abstract：:The MYC family of cellular oncogenes includes c-Myc, N-myc, and L-myc, which encode transcriptional regulators involved in the control of cell proliferation and death. Accordingly, these genes become aberrantly activated and expressed in specific types of cancers. For example, c-Myc translocations occur frequently in ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0812763106

    authors： Gostissa M,Ranganath S,Bianco JM,Alt FW

    更新日期：2009-02-17 00:00:00

  • Enhancement of dendritic cell activation via CD40 ligand-expressing γδ T cells is responsible for protective immunity to Plasmodium parasites.

    abstract：:Previous reports have shown that γδ T cells are important for the elimination of malaria parasites in humans and mice. However, how γδ T cells are involved in protective immunity against blood-stage malaria remains unknown. We infected γδ T-cell-deficient (TCRδ-KO) mice and control wild-type mice with Plasmodium bergh...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1204480109

    authors： Inoue S,Niikura M,Takeo S,Mineo S,Kawakami Y,Uchida A,Kamiya S,Kobayashi F

    更新日期：2012-07-24 00:00:00

  • Lipid bilayer mediates ion-channel cooperativity in a model of hair-cell mechanotransduction.

    abstract：:Mechanoelectrical transduction in the inner ear is a biophysical process underlying the senses of hearing and balance. The key players involved in this process are mechanosensitive ion channels. They are located in the stereocilia of hair cells and opened by the tension in specialized molecular springs, the tip links,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1713135114

    authors： Gianoli F,Risler T,Kozlov AS

    更新日期：2017-12-19 00:00:00

  • Microneedle-array patches loaded with hypoxia-sensitive vesicles provide fast glucose-responsive insulin delivery.

    abstract：:A glucose-responsive "closed-loop" insulin delivery system mimicking the function of pancreatic cells has tremendous potential to improve quality of life and health in diabetics. Here, we report a novel glucose-responsive insulin delivery device using a painless microneedle-array patch ("smart insulin patch") containi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1505405112

    authors： Yu J,Zhang Y,Ye Y,DiSanto R,Sun W,Ranson D,Ligler FS,Buse JB,Gu Z

    更新日期：2015-07-07 00:00:00

  • A putative molecular-activation switch in the transmembrane domain of erbB2.

    abstract：:Overexpression of the receptor tyrosine kinase (RTK) erbB2 (also designated neu or HER2) was implicated in causing a variety of human cancers, including mammary and ovarian carcinomas. Ligand-induced receptor dimerization is critical for stimulation of the intrinsic protein tyrosine kinase (PTK) of RTKs. It was theref...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.252640799

    authors： Fleishman SJ,Schlessinger J,Ben-Tal N

    更新日期：2002-12-10 00:00:00

  • Nuclear quantum effect with pure anharmonicity and the anomalous thermal expansion of silicon.

    abstract：:Despite the widespread use of silicon in modern technology, its peculiar thermal expansion is not well understood. Adapting harmonic phonons to the specific volume at temperature, the quasiharmonic approximation, has become accepted for simulating the thermal expansion, but has given ambiguous interpretations for micr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1707745115

    authors： Kim DS,Hellman O,Herriman J,Smith HL,Lin JYY,Shulumba N,Niedziela JL,Li CW,Abernathy DL,Fultz B

    更新日期：2018-02-27 00:00:00

  • An unusual structural motif of antimicrobial peptides containing end-to-end macrocycle and cystine-knot disulfides.

    abstract：:Four macrocyclic cystine-knot peptides of 29-31 residues, kalata, circulin A and B (CirA and CirB), and cyclopsychotride, have been isolated from coffee plants but have undetermined physiological functions. These macrocycles and 10 of their analogs prepared by chemical synthesis were tested against nine strains of mic...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.16.8913

    authors： Tam JP,Lu YA,Yang JL,Chiu KW

    更新日期：1999-08-03 00:00:00

  • Kinetic mechanism for HIV-1 neutralization by antibody 2G12 entails reversible glycan binding that slows cell entry.

    abstract：:Despite structural knowledge of broadly neutralizing monoclonal antibodies (NMAbs) complexed to HIV-1 gp120 and gp41 envelope glycoproteins, virus inactivation mechanisms have been difficult to prove, in part because neutralization assays are complex and were previously not understood. Concordant with recent evidence ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1109728109

    authors： Platt EJ,Gomes MM,Kabat D

    更新日期：2012-05-15 00:00:00

  • Isolation and characterization of human cDNA clones encoding a high mobility group box protein that recognizes structural distortions to DNA caused by binding of the anticancer agent cisplatin.

    abstract：:Human cDNA clones encoding a structure-specific recognition protein, SSRP1, that binds specifically to DNA modified with cisplatin have been isolated and characterized. The SSRP1 gene maps to human chromosome 11q12. The cDNA clones, obtained by using partial-length cDNAs described previously, predict an 81-kDa protein...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.6.2307

    authors： Bruhn SL,Pil PM,Essigmann JM,Housman DE,Lippard SJ

    更新日期：1992-03-15 00:00:00

  • Methyltransferases of gentamicin biosynthesis.

    abstract：:Gentamicin C complex from Micromonospora echinospora remains a globally important antibiotic, and there is revived interest in the semisynthesis of analogs that might show improved therapeutic properties. The complex consists of five components differing in their methylation pattern at one or more sites in the molecul...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1711603115

    authors： Li S,Guo J,Reva A,Huang F,Xiong B,Liu Y,Deng Z,Leadlay PF,Sun Y

    更新日期：2018-02-06 00:00:00

  • LsrF, a coenzyme A-dependent thiolase, catalyzes the terminal step in processing the quorum sensing signal autoinducer-2.

    abstract：:The quorum sensing signal autoinducer-2 (AI-2) regulates important bacterial behaviors, including biofilm formation and the production of virulence factors. Some bacteria, such as Escherichia coli, can quench the AI-2 signal produced by a variety of species present in the environment, and thus can influence AI-2-depen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1408691111

    authors： Marques JC,Oh IK,Ly DC,Lamosa P,Ventura MR,Miller ST,Xavier KB

    更新日期：2014-09-30 00:00:00

  • IKKβ is an IRF5 kinase that instigates inflammation.

    abstract：:The transcription factor interferon regulatory factor 5 (IRF5) is essential for the induction of inflammatory cytokines, but the mechanism by which IRF5 is activated is not well understood. Here we present evidence that the kinase IKKβ phosphorylates and activates IRF5 in response to stimulation in several inflammator...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1418516111

    authors： Ren J,Chen X,Chen ZJ

    更新日期：2014-12-09 00:00:00

  • Transcellular degradation of axonal mitochondria.

    abstract：:It is generally accepted that healthy cells degrade their own mitochondria. Here, we report that retinal ganglion cell axons of WT mice shed mitochondria at the optic nerve head (ONH), and that these mitochondria are internalized and degraded by adjacent astrocytes. EM demonstrates that mitochondria are shed through f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1404651111

    authors： Davis CH,Kim KY,Bushong EA,Mills EA,Boassa D,Shih T,Kinebuchi M,Phan S,Zhou Y,Bihlmeyer NA,Nguyen JV,Jin Y,Ellisman MH,Marsh-Armstrong N

    更新日期：2014-07-01 00:00:00