当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Transcellular degradation of axonal mitochondria.

Transcellular degradation of axonal mitochondria.

Abstract：

:It is generally accepted that healthy cells degrade their own mitochondria. Here, we report that retinal ganglion cell axons of WT mice shed mitochondria at the optic nerve head (ONH), and that these mitochondria are internalized and degraded by adjacent astrocytes. EM demonstrates that mitochondria are shed through formation of large protrusions that originate from otherwise healthy axons. A virally introduced tandem fluorophore protein reporter of acidified mitochondria reveals that acidified axonal mitochondria originating from the retinal ganglion cell are associated with lysosomes within columns of astrocytes in the ONH. According to this reporter, a greater proportion of retinal ganglion cell mitochondria are degraded at the ONH than in the ganglion cell soma. Consistently, analyses of degrading DNA reveal extensive mtDNA degradation within the optic nerve astrocytes, some of which comes from retinal ganglion cell axons. Together, these results demonstrate that surprisingly large proportions of retinal ganglion cell axonal mitochondria are normally degraded by the astrocytes of the ONH. This transcellular degradation of mitochondria, or transmitophagy, likely occurs elsewhere in the CNS, because structurally similar accumulations of degrading mitochondria are also found along neurites in superficial layers of the cerebral cortex. Thus, the general assumption that neurons or other cells necessarily degrade their own mitochondria should be reconsidered.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Davis CH,Kim KY,Bushong EA,Mills EA,Boassa D,Shih T,Kinebuchi M,Phan S,Zhou Y,Bihlmeyer NA,Nguyen JV,Jin Y,Ellisman MH,Marsh-Armstrong N

doi

10.1073/pnas.1404651111

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

9633-8

issue

26

eissn

0027-8424

issn

1091-6490

pii

1404651111

journal_volume

111

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Substrate conformational transitions in the active site of chorismate mutase: their role in the catalytic mechanism.

    abstract：:Chorismate mutase acts at the first branch-point of aromatic amino acid biosynthesis and catalyzes the conversion of chorismate to prephenate. The results of molecular dynamics simulations of the substrate in solution and in the active site of chorismate mutase are reported. Two nonreactive conformers of chorismate ar...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.141230998

    authors： Guo H,Cui Q,Lipscomb WN,Karplus M

    更新日期：2001-07-31 00:00:00

  • How cytotoxic T cells manage to discriminate nonself from self at the nonapeptide level.

    abstract：:Class I major histocompatibility complex (MHC) antigens are confronted with an apparently insurmountable dilemma. Each should show a binding preference to a common enough variety of nonapeptides, so that one relevant nonapeptide can be found in at least every other viral protein to provoke a cytotoxic T-cell response....

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.10.4643

    authors： Ohno S

    更新日期：1992-05-15 00:00:00

  • Predicting consumer behavior with Web search.

    abstract：:Recent work has demonstrated that Web search volume can "predict the present," meaning that it can be used to accurately track outcomes such as unemployment levels, auto and home sales, and disease prevalence in near real time. Here we show that what consumers are searching for online can also predict their collective...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1005962107

    authors： Goel S,Hofman JM,Lahaie S,Pennock DM,Watts DJ

    更新日期：2010-10-12 00:00:00

  • Efficient collective swimming by harnessing vortices through deep reinforcement learning.

    abstract：:Fish in schooling formations navigate complex flow fields replete with mechanical energy in the vortex wakes of their companions. Their schooling behavior has been associated with evolutionary advantages including energy savings, yet the underlying physical mechanisms remain unknown. We show that fish can improve thei...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1800923115

    authors： Verma S,Novati G,Koumoutsakos P

    更新日期：2018-06-05 00:00:00

  • Immunohistochemical localization of 3':5'-cyclic AMP and 3':5'-cyclic GMP in rat renal cortex: effect of parathyroid hormone.

    abstract：:Adenosine 3':5'-cyclic monophosphate (cAMP) and guanosine 3':5'-cyclic monophosphate (cGMP) were localized in cells of rat kidney cortex by an immunocytochemical technique before and after perfusion with parathyroid hormone (PTH). In control tissues the cAMP antiserum detected approximately the same intensity of fluor...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.74.8.3569

    authors： Dousa TP,Barnes LD,Ong SH,Steiner AL

    更新日期：1977-08-01 00:00:00

  • High-efficiency recovery of functional hematopoietic progenitor and stem cells from human cord blood cryopreserved for 15 years.

    abstract：:Transplanted cord blood (CB) hematopoietic stem cells (HSC) and progenitor cells (HPC) can treat malignant and nonmalignant disorders. Because long-term cryopreservation is critical for CB banking and transplantation, we assessed the efficiency of recovery of viable HSCHPC from individual CBs stored frozen for 15 yr. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0237086100

    authors： Broxmeyer HE,Srour EF,Hangoc G,Cooper S,Anderson SA,Bodine DM

    更新日期：2003-01-21 00:00:00

  • Up-regulation of FOXO1 and reduced inflammation by β-hydroxybutyric acid are essential diet restriction benefits against liver injury.

    abstract：:Liver ischemia and reperfusion injury (IRI) is a major challenge in liver surgery. Diet restriction reduces liver damage by increasing stress resistance; however, the underlying molecular mechanisms remain unclear. We investigated the preventive effect of 12-h fasting on mouse liver IRI. Partial warm hepatic IRI model...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1820282116

    authors： Miyauchi T,Uchida Y,Kadono K,Hirao H,Kawasoe J,Watanabe T,Ueda S,Okajima H,Terajima H,Uemoto S

    更新日期：2019-07-02 00:00:00

  • Hidden resources in the Escherichia coli genome restore PLP synthesis and robust growth after deletion of the essential gene pdxB.

    abstract：:PdxB (erythronate 4-phosphate dehydrogenase) is expected to be required for synthesis of the essential cofactor pyridoxal 5'-phosphate (PLP) in Escherichia coli Surprisingly, incubation of the ∆pdxB strain in medium containing glucose as a sole carbon source for 10 d resulted in visible turbidity, suggesting that PLP ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1915569116

    authors： Kim J,Flood JJ,Kristofich MR,Gidfar C,Morgenthaler AB,Fuhrer T,Sauer U,Snyder D,Cooper VS,Ebmeier CC,Old WM,Copley SD

    更新日期：2019-11-26 00:00:00

  • Dual origin of highly social behavior among bees.

    abstract：:A study of behavior and structure indicates that highly eusocial behavior arose twice in the bees--i.e., in the stingless bees (Meliponinae) and in the honeybees (Apinae). Morphological features demonstrate the distinctiveness of these two groups and the relationship of the latter to bumblebees (Bombini) and orchid be...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.74.3.1135

    authors： Winston ML,Michener CD

    更新日期：1977-03-01 00:00:00

  • Leukotriene B5 is formed in human neutrophils after dietary supplementation with icosapentaenoic acid.

    abstract：:Incorporation and conversion of icosapentaenoic acid (20:5, n - 3) by human polymorphonuclear leukocytes were studied in volunteers (n = 6) ingesting a normal Western diet supplemented with icosapentaenoic acid (approximately equal to 4 g daily). Ingestion of icosapentaenoic acid leads to formation of biologically les...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.5.1540

    authors： Strasser T,Fischer S,Weber PC

    更新日期：1985-03-01 00:00:00

  • miR-200 family and targets, ZEB1 and ZEB2, modulate uterine quiescence and contractility during pregnancy and labor.

    abstract：:Throughout most of pregnancy, uterine quiescence is maintained by increased progesterone receptor (PR) transcriptional activity, whereas spontaneous labor is initiated/facilitated by a concerted series of biochemical events that activate inflammatory pathways and have a negative impact on PR function. In this study, w...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1008301107

    authors： Renthal NE,Chen CC,Williams KC,Gerard RD,Prange-Kiel J,Mendelson CR

    更新日期：2010-11-30 00:00:00

  • The redshift-distance relation.

    abstract：:Key predictions of the Hubble law are inconsistent with direct observations on equitable complete samples of extragalactic sources in the optical, infrared, and x-ray wave bands-e.g., the predicted dispersion in apparent magnitude is persistently greatly in excess of its observed value, precluding an explanation via h...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.11.4798

    authors： Segal IE

    更新日期：1993-06-01 00:00:00

  • H-NOX-mediated nitric oxide sensing modulates symbiotic colonization by Vibrio fischeri.

    abstract：:The bioluminescent bacterium Vibrio fischeri initiates a specific, persistent symbiosis in the light organ of the squid Euprymna scolopes. During the early stages of colonization, V. fischeri is exposed to host-derived nitric oxide (NO). Although NO can be both an antimicrobial component of innate immunity and a key s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1003571107

    authors： Wang Y,Dufour YS,Carlson HK,Donohue TJ,Marletta MA,Ruby EG

    更新日期：2010-05-04 00:00:00

  • Basal and adenosine receptor-stimulated levels of cAMP are reduced in lymphocytes from alcoholic patients.

    abstract：:Alcoholism causes serious neurologic disease that may be due, in part, to the ability of ethanol to interact with neural cell membranes and change neuronal function. Adenosine receptors are membrane-bound proteins that appear to mediate some of the effects of ethanol in the brain. Human lymphocytes also have adenosine...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.5.1413

    authors： Diamond I,Wrubel B,Estrin W,Gordon A

    更新日期：1987-03-01 00:00:00

  • A generation at risk: young investigators and the future of the biomedical workforce.

    abstract：:A number of distressing trends, including a decline in the share of key research grants going to younger scientists, as well as a steady rise in the age at which investigators receive their first funding, are now a decades-long feature of the US biomedical research workforce. Working committees have proposed recommend...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1418761112

    authors： Daniels RJ

    更新日期：2015-01-13 00:00:00

  • Failure of glucose to elicit a normal secretory response in fetal pancreatic beta cells results from glucose insensitivity of the ATP-regulated K+ channels.

    abstract：:Fetal pancreatic beta cells demonstrate a deficient insulin release in response to glucose, but the underlying mechanism at the cellular level is unknown. By using beta cells from 21-day fetal rats we made an attempt to clarify the mechanism(s) behind this reduced glucose response. In addition to measuring insulin rel...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.12.4505

    authors： Rorsman P,Arkhammar P,Bokvist K,Hellerström C,Nilsson T,Welsh M,Welsh N,Berggren PO

    更新日期：1989-06-01 00:00:00

  • Effects of gamma-aminobutyric acid on skate retinal horizontal cells: evidence for an electrogenic uptake mechanism.

    abstract：:In the retinae of many vertebrates, there are classes of horizontal cell that probably utilize gamma-aminobutyric acid (GABA) as a neurotransmitter. As with other amino acid transmitter agents, the postsynaptic action of GABA is thought to be terminated by uptake into neurons and glia surrounding the release site. The...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.22.8945

    authors： Malchow RP,Ripps H

    更新日期：1990-11-01 00:00:00

  • Junctophilin-4, a component of the endoplasmic reticulum-plasma membrane junctions, regulates Ca2+ dynamics in T cells.

    abstract：:Orai1 and stromal interaction molecule 1 (STIM1) mediate store-operated Ca(2+) entry (SOCE) in immune cells. STIM1, an endoplasmic reticulum (ER) Ca(2+) sensor, detects store depletion and interacts with plasma membrane (PM)-resident Orai1 channels at the ER-PM junctions. However, the molecular composition of these ju...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1524229113

    authors： Woo JS,Srikanth S,Nishi M,Ping P,Takeshima H,Gwack Y

    更新日期：2016-03-08 00:00:00

  • Manganese and zinc blockade of enzyme induction: studies with microsomal heme oxygenase.

    abstract：:Heme oxygenase (decyclizing) [heme,hydrogen-donor:oxygen oxidoreductase (alpha-methene-oxidizing, hydroxylating), EC 1.14.99.3] is greatly induced in the kidney by the administration of nickel or tin. Manganese, when administered simultaneously with nickel or tin in an equimolar amount, substantially inhibited the ind...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.10.5331

    authors： Drummond GS,Kappas A

    更新日期：1979-10-01 00:00:00

  • Precise CRISPR/Cas9 editing of the NHE1 gene renders chickens resistant to the J subgroup of avian leukosis virus.

    abstract：:Avian leukosis virus subgroup J (ALV-J) is an important concern for the poultry industry. Replication of ALV-J depends on a functional cellular receptor, the chicken Na+/H+ exchanger type 1 (chNHE1). Tryptophan residue number 38 of chNHE1 (W38) in the extracellular portion of this molecule is a critical amino acid for...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1913827117

    authors： Koslová A,Trefil P,Mucksová J,Reinišová M,Plachý J,Kalina J,Kučerová D,Geryk J,Krchlíková V,Lejčková B,Hejnar J

    更新日期：2020-01-28 00:00:00

  • Systematic transcriptome analysis reveals tumor-specific isoforms for ovarian cancer diagnosis and therapy.

    abstract：:Tumor-specific molecules are needed across diverse areas of oncology for use in early detection, diagnosis, prognosis and therapy. Large and growing public databases of transcriptome sequencing data (RNA-seq) derived from tumors and normal tissues hold the potential of yielding tumor-specific molecules, but because th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1508057112

    authors： Barrett CL,DeBoever C,Jepsen K,Saenz CC,Carson DA,Frazer KA

    更新日期：2015-06-09 00:00:00

  • Vaccine protection against simian immunodeficiency virus infection.

    abstract：:Rhesus monkeys were immunized by multiple inoculations with purified, disrupted, noninfectious simian immunodeficiency virus (SIV) in adjuvant. Immunized monkeys developed anti-SIV antibodies detectable by whole-virus ELISA and by immunoblot reactivity; these antibodies had weak neutralizing activity. One week after t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.16.6353

    authors： Desrosiers RC,Wyand MS,Kodama T,Ringler DJ,Arthur LO,Sehgal PK,Letvin NL,King NW,Daniel MD

    更新日期：1989-08-01 00:00:00

  • Brominated 7-hydroxycoumarin-4-ylmethyls: photolabile protecting groups with biologically useful cross-sections for two photon photolysis.

    abstract：:Photochemical release (uncaging) of bioactive messengers with three-dimensional spatial resolution in light-scattering media would be greatly facilitated if the photolysis could be powered by pairs of IR photons rather than the customary single UV photons. The quadratic dependence on light intensity would confine the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.4.1193

    authors： Furuta T,Wang SS,Dantzker JL,Dore TM,Bybee WJ,Callaway EM,Denk W,Tsien RY

    更新日期：1999-02-16 00:00:00

  • A new form of cerebellar long-term potentiation is postsynaptic and depends on nitric oxide but not cAMP.

    abstract：:Long-term depression (LTD) at cerebellar parallel fiber (PF)-Purkinje cell synapses must be balanced by long-term potentiation (LTP) to prevent saturation and allow reversal of motor learning. The only previously analyzed form of cerebellar LTP is induced by 4-8 Hz PF stimulation and requires cAMP but not nitric oxide...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.122206399

    authors： Lev-Ram V,Wong ST,Storm DR,Tsien RY

    更新日期：2002-06-11 00:00:00

  • Early effect of interferon on mouse leukemia cells cultivated in a chemostat.

    abstract：:Mouse interferon preparations inhibited the multiplication of mouse leukemia L 1210 cells cultivated under steady-state conditions in a chemostat. The use of this sensitive and controlled system led to the detection of a rapid inhibition in the incorporation of (3-H)thymidine into cellular acid-precipitable material 2...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.6.2265

    authors： Tovey M,Brouty-Boyé D,Gresser I

    更新日期：1975-06-01 00:00:00

  • Amino acid residues essential for biological activity of a peptide derived from a major histocompatibility complex class I antigen.

    abstract：:The stimulatory activity of peptides from the alpha 1 domain of the major histocompatibility complex (MHC) class I antigen on adipose cell glucose transport was previously shown to require a preformed, ordered conformation of the peptide. The two peptides studied previously were Dk-(61-85) (ERETQIAKGNEQSFRVDLRTLLRYY) ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.16.7686

    authors： Stagsted J,Mapelli C,Meyers C,Matthews BW,Anfinsen CB,Goldstein A,Olsson L

    更新日期：1993-08-15 00:00:00

  • Postcranial morphology of the middle Pleistocene humans from Sima de los Huesos, Spain.

    abstract：:Current knowledge of the evolution of the postcranial skeleton in the genus Homo is hampered by a geographically and chronologically scattered fossil record. Here we present a complete characterization of the postcranium of the middle Pleistocene paleodeme from the Sima de los Huesos (SH) and its paleobiological impli...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1514828112

    authors： Arsuaga JL,Carretero JM,Lorenzo C,Gómez-Olivencia A,Pablos A,Rodríguez L,García-González R,Bonmatí A,Quam RM,Pantoja-Pérez A,Martínez I,Aranburu A,Gracia-Téllez A,Poza-Rey E,Sala N,García N,Alcázar de Velasco A,Cuenca-B

    更新日期：2015-09-15 00:00:00

  • Evolution of carbon sinks in a changing climate.

    abstract：:Climate change is expected to influence the capacities of the land and oceans to act as repositories for anthropogenic CO2 and hence provide a feedback to climate change. A series of experiments with the National Center for Atmospheric Research-Climate System Model 1 coupled carbon-climate model shows that carbon sink...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0504949102

    authors： Fung IY,Doney SC,Lindsay K,John J

    更新日期：2005-08-09 00:00:00

  • Chemical synthesis and X-ray structure of a heterochiral {D-protein antagonist plus vascular endothelial growth factor} protein complex by racemic crystallography.

    abstract：:Total chemical synthesis was used to prepare the mirror image (D-protein) form of the angiogenic protein vascular endothelial growth factor (VEGF-A). Phage display against D-VEGF-A was used to screen designed libraries based on a unique small protein scaffold in order to identify a high affinity ligand. Chemically syn...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1210483109

    authors： Mandal K,Uppalapati M,Ault-Riché D,Kenney J,Lowitz J,Sidhu SS,Kent SB

    更新日期：2012-09-11 00:00:00

  • Enrichment for RNA molecules that bind a Diels-Alder transition state analog.

    abstract：:RNA molecules that bind a transition state analog for a Diels-Alder reaction (Kd = 0.35 +/- 0.05 mM) were isolated from a starting pool of approximately 10(14) sequences by affinity chromatography. After the initial rise and plateau of the amount of RNA that eluted with soluble analog, a step gradient elution was used...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.26.13028

    authors： Morris KN,Tarasow TM,Julin CM,Simons SL,Hilvert D,Gold L

    更新日期：1994-12-20 00:00:00