A putative molecular-activation switch in the transmembrane domain of erbB2.

abstract：:Fodrin, a protein composed of two polypeptides with molecular weights of 250,000 and 240,000, is concentrated in the cortical cytoplasm of neurons, and moves down the axons by the process of axonal transport. We have used immunofluorescence techniques to determine whether fodrin antigens also move in non-neuronal cell...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levine J,Willard M

更新日期：1983-01-01 00:00:00

abstract：:Earlier Monte Carlo studies on a single-helix model of the GTP cap at the end of a microtubule are extended here to a more realistic five-start helix model of the microtubule end. As in the earlier work, phase changes occur at the microtubule end: the end is either capped with GTP and growing slowly or uncapped and sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen YD,Hill TL

更新日期：1985-02-01 00:00:00

abstract：:The mitochondrial trifunctional protein (TFP) catalyzes three reactions in the fatty acid β-oxidation process. Mutations in the two TFP subunits cause mitochondrial trifunctional protein deficiency and acute fatty liver of pregnancy that can lead to death. Here we report a 4.2-Å cryo-electron microscopy α2β2 tetrameri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang K,Li N,Wang X,Dai J,Liu P,Wang C,Chen XW,Gao N,Xiao J

更新日期：2018-07-03 00:00:00

abstract：:Circadian rhythms in metazoan eukaryotes are controlled by an endogenous molecular clock. It functions in many locations, including subsets of brain neurons (clock neurons) within the central nervous system. Although the molecular clock relies on transcription/translation feedback loops, posttranscriptional regulation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Rosbash M

更新日期：2016-05-24 00:00:00

abstract：:A photoreactive derivative of epidermal growth factor (EGF) has been used to identify and specifically label a membrane receptor for EGF on mouse 3T3 cells. Photoactivable EGF, labeled with 125I, was incubated with 3T3 cells and then photolyzed in situ to generate a nitrene capable of reacting with a wide variety of c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Das M,Miyakawa T,Fox CF,Pruss RM,Aharonov A,Herschman HR

更新日期：1977-07-01 00:00:00

abstract：:Glutamate transport by the excitatory amino acid carrier EAAC1 is known to be reversible. Thus, glutamate can either be taken up into cells, or it can be released from cells through reverse transport, depending on the electrochemical gradient of the co- and countertransported ions. However, it is unknown how fast and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Z,Tao Z,Gameiro A,Barcelona S,Braams S,Rauen T,Grewer C

更新日期：2007-11-13 00:00:00

abstract：:Transcriptional regulation by nuclear hormone receptors is thought to involve interactions with putative cofactors that may potentiate receptor function. Here we show that human thyroid hormone receptor alpha purified from HeLa cells grown in the presence of thyroid hormone (T3) is associated with a group of distinct ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fondell JD,Ge H,Roeder RG

更新日期：1996-08-06 00:00:00

abstract：:Cellular factors tightly regulate the architecture of bundles of filamentous cytoskeletal proteins, giving rise to assemblies with distinct morphologies and physical properties, and a similar control of the supramolecular organization of nanotubes and nanorods in synthetic materials is highly desirable. However, it is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Needleman DJ,Ojeda-Lopez MA,Raviv U,Miller HP,Wilson L,Safinya CR

更新日期：2004-11-16 00:00:00

abstract：:The TATA box-binding activity of transcription factor IID (TFIID) is autoinhibited by the N-terminal domain of the Drosophila TATA box-binding protein- (TBP) associated factor 230/yeast TBP-associated factor 145 subunit, which binds to the TATA box-binding domain of TBP by mimicking the TATA box structure. Here, we pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kotani T,Banno K,Ikura M,Hinnebusch AG,Nakatani Y,Kawaichi M,Kokubo T

更新日期：2000-06-20 00:00:00

abstract：:Patent ductus arteriosus (PDA) is a common congenital heart disease that results when the ductus arteriosus, a muscular artery, fails to remodel and close after birth. A syndromic form of this disorder, Char syndrome, is caused by mutation in TFAP2B, the gene encoding a neural crest-derived transcription factor. Estab...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mani A,Radhakrishnan J,Farhi A,Carew KS,Warnes CA,Nelson-Williams C,Day RW,Pober B,State MW,Lifton RP

更新日期：2005-02-22 00:00:00

abstract：:The RuvC protein of Escherichia coli catalyzes the resolution of recombination intermediates during genetic recombination and the recombinational repair of damaged DNA. Resolution involves specific recognition of the Holliday structure to form a complex that exhibits twofold symmetry with the DNA in an open configurat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bennett RJ,West SC

更新日期：1996-10-29 00:00:00

abstract：:We stabilize nematic droplets with handles against surface tension-driven instabilities, using a yield-stress material as outer fluid, and study the complex nematic textures and defect structures that result from the competition between topological constraints and the elasticity of the nematic liquid crystal. We uncov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pairam E,Vallamkondu J,Koning V,van Zuiden BC,Ellis PW,Bates MA,Vitelli V,Fernandez-Nieves A

更新日期：2013-06-04 00:00:00

abstract：:Recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) was found to increase the adherence of purified peripheral blood monocytes to plastic surfaces and to monolayers of human umbilical vein endothelial cells. With plastic surfaces as a model 9-hr culture with GM-CSF was necessary for enhancement...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gamble JR,Elliott MJ,Jaipargas E,Lopez AF,Vadas MA

更新日期：1989-09-01 00:00:00

abstract：:The possible role of guanosine 5'-triphosphate,3'-diphosphate (pppGpp) in protein synthesis by Escherichia coli ribosomes and protein factors was examined. Although pppGpp could effectively substitute for GTP in reactions catalyzed by initiation factor 2 (ribosomal binding of fMet-tRNA and formation of N-formylmethion...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hamel E,Cashel M

更新日期：1973-11-01 00:00:00

abstract：:Cytoplasmic RNA extracted from human tissue culture cells infected with adenovirus type 2 was used to program protein synthesis in a cell-free system derived from mammalian cells. Analysis of the protein product by polyacrylamide gel electrophoresis revealed ten adenovirus-specific polypeptides. Five of these were fur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson CW,Lewis JB,Atkins JF,Gesteland RF

更新日期：1974-07-01 00:00:00

abstract：:The Nod-like receptor family in man contains proteins that recognize invasive bacteria. Nod1, a member of this family, is activated by specific peptidoglycan-derived muropeptides that contain meso-diaminopimelic acid. Plants contain a large family of proteins known as resistance (R) proteins that have common structura...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： da Silva Correia J,Miranda Y,Leonard N,Ulevitch R

更新日期：2007-04-17 00:00:00

abstract：:Although gamma/delta T cells express numerous in vitro functions similar to alpha/beta T cells, little is known about their biological functioning in vivo. Furthermore, it is unclear whether alpha/beta T cells and gamma/delta T cells act independently or in a coordinated way. In the present study, gamma/delta T cells ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaufmann SH,Blum C,Yamamoto S

更新日期：1993-10-15 00:00:00

abstract：:The nucleus accumbens is a brain region in which limbic and motor inputs converge. How these information modalities shape accumbens output is not clearly understood. Here, we report that synaptic inputs from the prefrontal cortex and limbic structures interact differently depending on their timing. Coincident inputs m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goto Y,O'Donnell P

更新日期：2002-10-01 00:00:00

abstract：:"Head-to-head" terpene synthases catalyze the first committed steps in sterol and carotenoid biosynthesis: the condensation of two isoprenoid diphosphates to form cyclopropylcarbinyl diphosphates, followed by ring opening. Here, we report the structures of Staphylococcus aureus dehydrosqualene synthase (CrtM) complexe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin FY,Liu CI,Liu YL,Zhang Y,Wang K,Jeng WY,Ko TP,Cao R,Wang AH,Oldfield E

更新日期：2010-12-14 00:00:00

abstract：:ExoU is a type III-secreted cytotoxin expressing A2 phospholipase activity when injected into eukaryotic target cells by the bacterium Pseudomonas aeruginosa The enzymatic activity of ExoU is undetectable in vitro unless ubiquitin, a required cofactor, is added to the reaction. The role of ubiquitin in facilitating Ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tessmer MH,Anderson DM,Pickrum AM,Riegert MO,Moretti R,Meiler J,Feix JB,Frank DW

更新日期：2018-01-16 00:00:00

abstract：:The tau and gamma subunits of DNA polymerase III holoenzyme are both products of the dnaX gene. Since tau and gamma are required as stoichiometric components of the replicative complex, a mechanism must exist for the cell to coordinate their synthesis and ensure that both subunits are present in an adequate quantity a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flower AM,McHenry CS

更新日期：1990-05-01 00:00:00

abstract：:The "disease-specific" (dsp) region next to the hrp gene cluster of Erwinia amylovora is required for pathogenicity but not for elicitation of the hypersensitive reaction. A 6.6-kb apparent operon, dspEF, was found responsible for this phenotype. The operon contains genes dspE and dspF and is positively regulated by h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bogdanove AJ,Kim JF,Wei Z,Kolchinsky P,Charkowski AO,Conlin AK,Collmer A,Beer SV

更新日期：1998-02-03 00:00:00

abstract：:The breast and ovarian cancer suppressor BRCA2 controls the enzyme RAD51 during homologous DNA recombination (HDR) to preserve genome stability. BRCA2 binds to RAD51 through 8 conserved BRC repeat motifs dispersed in an 1127-residue region (BRCA2([BRC1-8])). Here, we show that BRCA2([BRC1-8]) exerts opposing effects o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shivji MK,Mukund SR,Rajendra E,Chen S,Short JM,Savill J,Klenerman D,Venkitaraman AR

更新日期：2009-08-11 00:00:00

abstract：:A Mr 70,000 protein was isolated from cytotoxic human large granular lymphocytes and shown to have cytotoxic activity. The protein was demonstrated to be immunochemically related to the ninth component (C9) of complement and was therefore designated C9-related protein (C9RP). This finding suggests that C9RP and C9 sha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zalman LS,Brothers MA,Chiu FJ,Müller-Eberhard HJ

更新日期：1986-07-01 00:00:00

abstract：:Thiol-dependent redox regulation allows the rapid adaptation of chloroplast function to unpredictable changes in light intensity. Traditionally, it has been considered that chloroplast redox regulation relies on photosynthetically reduced ferredoxin (Fd), thioredoxins (Trxs), and an Fd-dependent Trx reductase (FTR), t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pérez-Ruiz JM,Naranjo B,Ojeda V,Guinea M,Cejudo FJ

更新日期：2017-11-07 00:00:00

abstract：:A five-helix secondary structural model is proposed for eukaryotic cytoplasmic 5S rRNA. All available sequence data are consistent with this model including those from Chlorella 5S rRNA whose sequence is revised by data included here. Various architectural features of eukaryotic 5S rRNA are summarized in terms of this...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luehrsen KR,Fox GE

更新日期：1981-04-01 00:00:00

abstract：:We have selected from a mixture of random polynucleotides an RNA that is able to replicate in the presence of the DNA-dependent RNA polymerase of Escherichia coli, provided the medium contains Mn(2+) and ITP, in addition to ATP, CTP, and UTP. The RNA consists of a chain of about 125 (+/-25) nucleotides and appears to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biebricher CK,Orgel LE

更新日期：1973-03-01 00:00:00

abstract：:Biochemical evidence is presented for the disintegration of the lens fiber plasma membrane in human cataracts. The intrinsic membrane proteins are found in both the water-soluble and water-insoluble nonmembrane fractions of the cataract lens but not in the normal tissue. Furthermore, in contrast to the normal lens, no...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garner MH,Roy D,Rosenfeld L,Garner WH,Spector A

更新日期：1981-03-01 00:00:00

abstract：:Two RNA fractions have been isolated and purified from both oncogenic and nononcogenic strains of Agrobacterium tumefaciens. Both RNAs are capable of inducing the formation of transplantable tumors when introduced at wound sites in stems of Datura stramonium plants. One of these RNA fractions was found to be bound to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beljanski M,Aaron-da Cunha MI,Beljanski MS,Manigault P,Bourgarel P

更新日期：1974-05-01 00:00:00

abstract：:Cullin-Ring E3 ubiquitin ligases target substrates for ubiquitin-dependent, proteasome-mediated degradation and regulate critical cellular processes. These cullins assemble with cellular substrate receptor proteins through specific adaptor molecules. F-box- and BC-box-containing receptors use Skp1, ElonginB, and Elong...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luo K,Xiao Z,Ehrlich E,Yu Y,Liu B,Zheng S,Yu XF

更新日期：2005-08-09 00:00:00