Specific radiolabeling of a cell surface receptor for epidermal growth factor.

abstract：:Loquacious-PD (Loqs-PD) is required for biogenesis of many endogenous siRNAs in Drosophila In vitro, Loqs-PD enhances the rate of dsRNA cleavage by Dicer-2 and also enables processing of substrates normally refractory to cleavage. Using purified components, and Loqs-PD truncations, we provide a mechanistic basis for L...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Trettin KD,Sinha NK,Eckert DM,Apple SE,Bass BL

更新日期：2017-09-19 00:00:00

abstract：:Whole human blood was examined by (31)P nuclear magnetic resonance spectroscopy. Individual phosphates (alpha,beta,gamma) of ATP were identifiable, and two microenvironments appeared to be present for this molecule. When sequential recordings of freshly collected blood were made, 2,3-diphosphoglycerate was observed to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henderson TO,Costello AJ,Omachi A

更新日期：1974-06-01 00:00:00

abstract：:The DEMETER (DME) DNA glycosylase initiates active DNA demethylation via the base-excision repair pathway and is vital for reproduction in Arabidopsis thaliana DME-mediated DNA demethylation is preferentially targeted to small, AT-rich, and nucleosome-depleted euchromatic transposable elements, influencing expression ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park JS,Frost JM,Park K,Ohr H,Park GT,Kim S,Eom H,Lee I,Brooks JS,Fischer RL,Choi Y

更新日期：2017-02-21 00:00:00

abstract：:Patients undergoing treatment at a community-based renal dialysis clinic were monitored monthly for hepatitis B surface antigen (HBsAg) and antibody to HBsAg (anti-HBs). Of 160 patients who began treatment HBsAg(-)/anti-HBs(-), 77 subsequently became HBsAg(+). Once HBsAg(+), males were more likely to remain HBsAg(+) i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： London WT,Drew JS

更新日期：1977-06-01 00:00:00

abstract：:Heparin-binding growth factor type 1 (HBGF-1; sometimes termed acidic fibroblast growth factor) is potentially an important factor in liver regeneration. HBGF-1 alone (half-maximal effect at 60 pM) stimulated hepatocyte DNA synthesis and bound to a high-affinity receptor (Kd = 62 pM; 5000 per cell). Epidermal growth f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kan M,Huang JS,Mansson PE,Yasumitsu H,Carr B,McKeehan WL

更新日期：1989-10-01 00:00:00

abstract：:The ability of the radiomimetic anticancer enediyne C-1027 to induce ataxia-telangiectasia mutated (ATM) and ATM and Rad3-related (ATR)-independent damage responses was discovered to reside in its unique ability to concurrently generate robust amounts of double-strand breaks (DSBs) and interstrand cross-links (ICLs) i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kennedy DR,Ju J,Shen B,Beerman TA

更新日期：2007-11-06 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is an emerging cause of catheter-associated urinary tract infection (CAUTI), which frequently progresses to more serious invasive infections. We adapted a mouse model of CAUTI to investigate how catheterization increases an individual's susceptibility to MRSA UTI. Thi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walker JN,Flores-Mireles AL,Pinkner CL,Schreiber HL 4th,Joens MS,Park AM,Potretzke AM,Bauman TM,Pinkner JS,Fitzpatrick JAJ,Desai A,Caparon MG,Hultgren SJ

更新日期：2017-10-10 00:00:00

abstract：:We have identified new genomic alterations in the breast cancer cell line HCC1954, using high-throughput transcriptome sequencing. With 120 Mb of cDNA sequences, we were able to identify genomic rearrangement events leading to fusions or truncations of genes including MRE11 and NSD1, genes already implicated in oncoge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao Q,Caballero OL,Levy S,Stevenson BJ,Iseli C,de Souza SJ,Galante PA,Busam D,Leversha MA,Chadalavada K,Rogers YH,Venter JC,Simpson AJ,Strausberg RL

更新日期：2009-02-10 00:00:00

abstract：:The secreted nodulation-signaling protein NodO was purified from the supernatant of cultures of Rhizobium leguminosarum biovar viciae. The native protein has a M(r) of approximately 67,000, suggesting that it exists as a dimer since the DNA sequence predicts a M(r) of 30,002. Pure NodO protein had no protease, pectina...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sutton JM,Lea EJ,Downie JA

更新日期：1994-10-11 00:00:00

abstract：:The catalytic cysteine of the typical 2-Cys Prx subfamily of peroxiredoxins is occasionally hyperoxidized to cysteine sulfinic acid during the peroxidase catalytic cycle. Sulfinic Prx (Prx-SO2H) is reduced back to the active form of the enzyme by sulfiredoxin. The abundance of Prx-SO2H was recently shown to oscillate ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cho CS,Yoon HJ,Kim JY,Woo HA,Rhee SG

更新日期：2014-08-19 00:00:00

abstract：:Two NADPH:cytochrome P450 oxidoreductases (CPRs) from parsley (Petroselinum crispum) were cloned, and the complete proteins were expressed and functionally identified in yeast. The two enzymes, designated CPR1 and CPR2, are 80% identical in amino acid sequence with one another and about 75% identical with CPRs from se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koopmann E,Hahlbrock K

更新日期：1997-12-23 00:00:00

abstract：:Detailed molecular definition of the WAGR region at chromosome 11p13 has been achieved by chromosome breakpoint analysis and long-range restriction mapping. Here we describe the molecular detection of a cytogenetically invisible 1-megabase deletion in an individual with aniridia, cryptorchidism, and hypospadias but no...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Heyningen V,Bickmore WA,Seawright A,Fletcher JM,Maule J,Fekete G,Gessler M,Bruns GA,Huerre-Jeanpierre C,Junien C

更新日期：1990-07-01 00:00:00

abstract：:The explosive growth in citizen science combined with a recalcitrance on the part of mainstream science to fully embrace this data collection technique demands a rigorous examination of the factors influencing data quality and project efficacy. Patterns of contributor effort and task performance have been well reviewe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parrish JK,Jones T,Burgess HK,He Y,Fortson L,Cavalier D

更新日期：2019-02-05 00:00:00

abstract：:Ho endonuclease of Saccharomyces cerevisiae is a homing endonuclease that makes a site-specific double-strand break in the MAT gene in late G(1). Here we show that Ho is rapidly degraded via the ubiquitin-26S proteasome system through two ubiquitin-conjugating enzymes UBC2(Rad6) and UBC3(Cdc34). UBC2(Rad6) is complexe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaplun L,Ivantsiv Y,Kornitzer D,Raveh D

更新日期：2000-08-29 00:00:00

abstract：:The role of spliced leader RNA (SL RNA) in trans-splicing in Caenorhabditis elegans has been studied through a combination of in vitro mutagenesis and in vivo complementation of rrs-1 mutant nematodes, which lack endogenous SL1 RNA. Three classes of mutant SL1 RNAs have been found-those that rescue the lethal phenotyp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xie H,Hirsh D

更新日期：1998-04-14 00:00:00

abstract：:The T helper type 2 (Th2) cytokine genes Il4, Il5, and Il13 are contained within a 140-kb region of mouse chromosome 11 and their expression is controlled by a locus control region (LCR) embedded within this locus. The LCR is composed of a number of DNase I-hypersensitive sites (HSs), which are believed to encompass t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams A,Lee GR,Spilianakis CG,Hwang SS,Eisenbarth SC,Flavell RA

更新日期：2013-04-23 00:00:00

abstract：:Bacillus anthracis, the etiologic agent of anthrax, avoids immune surveillance and commandeers host macrophages as a vehicle for lymphatic spreading. Here, we show that B. anthracis edema toxin (ET), via its adenylyl cyclase activity, dramatically increases the motility of infected macrophages and the expression of va...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim C,Wilcox-Adelman S,Sano Y,Tang WJ,Collier RJ,Park JM

更新日期：2008-04-22 00:00:00

abstract：:Aerogel materials have myriad scientific and technological applications due to their large intrinsic surface areas and ultralow densities. However, creating a nanodiamond aerogel matrix has remained an outstanding and intriguing challenge. Here we report the high-pressure, high-temperature synthesis of a diamond aerog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pauzauskie PJ,Crowhurst JC,Worsley MA,Laurence TA,Kilcoyne AL,Wang Y,Willey TM,Visbeck KS,Fakra SC,Evans WJ,Zaug JM,Satcher JH Jr

更新日期：2011-05-24 00:00:00

abstract：:A continuous fluorescence-based assay is described for measuring helicase-mediated unwinding of duplex DNA. The assay utilizes an oligonucleotide substrate containing the fluorescent adenine analog, 2-aminopurine, at regular intervals. 2-Aminopurine forms a Watson-Crick-type base pair with thymine and does not distort...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raney KD,Sowers LC,Millar DP,Benkovic SJ

更新日期：1994-07-05 00:00:00

abstract：:The involvement of gangliosides in processes related to nerve regeneration and sprouting has been demonstrated recently. The type of interaction by which gangliosides may influence neuronal sprouting was investigated in the present work. Affinity-purified rabbit anti-GM1 antibodies were found to block the sprouting fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwartz M,Spirman N

更新日期：1982-10-01 00:00:00

abstract：:The influence of language familiarity upon speaker identification is well established, to such an extent that it has been argued that "Human voice recognition depends on language ability" [Perrachione TK, Del Tufo SN, Gabrieli JDE (2011) Science 333(6042):595]. However, 7-mo-old infants discriminate speakers of their ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fleming D,Giordano BL,Caldara R,Belin P

更新日期：2014-09-23 00:00:00

abstract：:Promoter-proximal stalling, a general phenomenon observed during the expression of many RNA polymerase II transcribed genes, is dependent on transcription factor IIH (TFIIH). Reactions lacking TFIIH initiated transcription, but the transcription complex encountered a block to elongation proximal to the promoter. The a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kumar KP,Akoulitchev S,Reinberg D

更新日期：1998-08-18 00:00:00

abstract：:Although aquaporin 5 (AQP5) is the major water channel expressed in alveolar type I cells in the lung, its actual role in the lung is a matter of considerable speculation. By using immunohistochemical staining, we show that AQP5 expression in mouse lung is not restricted to type I cells, but is also detected in alveol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krane CM,Fortner CN,Hand AR,McGraw DW,Lorenz JN,Wert SE,Towne JE,Paul RJ,Whitsett JA,Menon AG

更新日期：2001-11-20 00:00:00

abstract：:IL-17-producing CD27(-) γδ cells (γδ(27-) cells) are widely viewed as innate immune cells that make critical contributions to host protection and autoimmunity. However, factors that promote them over IFN-γ-producing γδ(27+) cells are poorly elucidated. Moreover, although human IL-17-producing γδ cells are commonly imp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Michel ML,Pang DJ,Haque SF,Potocnik AJ,Pennington DJ,Hayday AC

更新日期：2012-10-23 00:00:00

abstract：:Cullin-Ring E3 ubiquitin ligases target substrates for ubiquitin-dependent, proteasome-mediated degradation and regulate critical cellular processes. These cullins assemble with cellular substrate receptor proteins through specific adaptor molecules. F-box- and BC-box-containing receptors use Skp1, ElonginB, and Elong...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luo K,Xiao Z,Ehrlich E,Yu Y,Liu B,Zheng S,Yu XF

更新日期：2005-08-09 00:00:00

abstract：:There is evidence that interstitial telomere (TTAGGG)n-like sequences at chromosome 2 fragile sites play an important role in the somatic events that characterize the earliest phases of radiation-induced acute myeloid leukemia in the CBA/H mouse. Here we show that the highly inbred CBA/H colony unexpectedly contains f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Silver A,Cox R

更新日期：1993-02-15 00:00:00

abstract：:Both neocortical and hippocampal networks organize the firing patterns of their neurons by prominent oscillations during sleep, but the functional role of these rhythms is not well understood. Here, we show a robust correlation of neuronal discharges between the somatosensory cortex and hippocampus on both slow and fi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sirota A,Csicsvari J,Buhl D,Buzsáki G

更新日期：2003-02-18 00:00:00

abstract：:It is well established that Ha-ras and c-myc genes collaborate in promoting transformation, tumor progression, and metastasis. However, the precise mechanism underlying this cooperation remains unclear. In the present study, we document that astrocyte elevated gene-1 (AEG-1) is a downstream target molecule of Ha-ras a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee SG,Su ZZ,Emdad L,Sarkar D,Fisher PB

更新日期：2006-11-14 00:00:00

abstract：:Protein misfolding is the molecular basis for several human diseases. How the primary amino acid sequence triggers misfolding and determines the benign or toxic character of the misfolded protein remains largely obscure. Among proteins that misfold, polyglutamine (polyQ) expansion proteins provide an interesting case:...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duennwald ML,Jagadish S,Muchowski PJ,Lindquist S

更新日期：2006-07-18 00:00:00

abstract：:Although major research efforts have focused on how specific components of foodstuffs affect health, relatively little is known about a more fundamental aspect of diet, the frequency and circadian timing of meals, and potential benefits of intermittent periods with no or very low energy intakes. The most common eating...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mattson MP,Allison DB,Fontana L,Harvie M,Longo VD,Malaisse WJ,Mosley M,Notterpek L,Ravussin E,Scheer FA,Seyfried TN,Varady KA,Panda S

更新日期：2014-11-25 00:00:00