Isolation of the tumor-inducing RNA from oncogenic and nononcogenic Agrobacterium tumefaciens.

abstract：:Diminished Sonic Hedgehog (Shh) signaling is associated with the most common forebrain defect in humans, holoprosencephaly (HPE), which includes cyclopia, a phenotype also seen in mice and other vertebrates with defective Shh signaling. The secreted protein Shh acts as a crucial factor that patterns the ventral forebr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roessler E,Du YZ,Mullor JL,Casas E,Allen WP,Gillessen-Kaesbach G,Roeder ER,Ming JE,Ruiz i Altaba A,Muenke M

更新日期：2003-11-11 00:00:00

abstract：:Most climate models are large dynamical systems involving a million (or more) variables on big computers. Given that they are nonlinear and not perfect, what can we expect to learn from them about the earth's climate? How can we determine which aspects of their output might be useful and which are noise? And how shoul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith LA

更新日期：2002-02-19 00:00:00

abstract：:Subunits A and B of chloroplast glyceraldehyde-3-phosphate dehydrogenase are synthesized as higher molecular weight precursors when polyadenylylated mRNA from angiosperm seedlings is translated in vitro by wheat germ ribosomes. The in vivo levels of mRNA coding for these precursors are strongly light dependent, and th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cerff R,Kloppstech K

更新日期：1982-12-01 00:00:00

abstract：:RNA synthesis by the paramyxovirus respiratory syncytial virus, a ubiquitous human pathogen, was found to be more complex than previously appreciated for the nonsegmented negative-strand RNA viruses. Intracellular RNA replication of a plasmid-encoded "minigenome" analog of viral genomic RNA was directed by coexpressio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collins PL,Hill MG,Cristina J,Grosfeld H

更新日期：1996-01-09 00:00:00

abstract：:The glucose transporter isoform GLUT4 is found only in cells that exhibit insulin-sensitive glucose transport. To investigate the function of this transporter, L6 myoblasts were stably transfected with GLUT4 cDNA. GLUT4 underwent insulin-dependent movement to the cell surface in myoblasts overexpressing the transporte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lawrence JC Jr,Piper RC,Robinson LJ,James DE

更新日期：1992-04-15 00:00:00

abstract：:We have compared the behavior in solution of the synthetic polynucleotide poly(dG-m5dC).poly(dG-m5dC) with that of the unmethylated polynucleotide poly(dG-dC).poly(dG-dC). In solutions containing high concentrations of salt, poly(dG-dC).poly(dG-dC) is known to exhibit altered circular dichroic and absorption spectra c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Behe M,Felsenfeld G

更新日期：1981-03-01 00:00:00

abstract：:Although rigorous measures of similarity for sequence and structure are now well established, the problem of defining functional relationships has been particularly daunting. Here, we present several manifold embedding techniques to compute distances between Gene Ontology (GO) functional annotations and consequently e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lerman G,Shakhnovich BE

更新日期：2007-07-03 00:00:00

abstract：:Heteroduplex molecules of pneumococcal DNA, prepared by cross-annealing resolved complementary strands, have been used as donors in transformation. A number of pairs of genetic markers are situated exclusively in the trans configuration in these heteroduplexes. Insertion of two markers from trans configuration into op...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roger M

更新日期：1972-02-01 00:00:00

abstract：:An src/yes-related novel gene named syn (SYN in human gene nomenclature) has been identified in the human genome on chromosome 6 and characterized by molecular cloning. Nucleotide sequence analysis of cDNA clones showed that the c-syn gene could encode a protein-tyrosine kinase that is very similar in primary structur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Semba K,Nishizawa M,Miyajima N,Yoshida MC,Sukegawa J,Yamanashi Y,Sasaki M,Yamamoto T,Toyoshima K

更新日期：1986-08-01 00:00:00

abstract：:Because of the insolubility and polymeric properties of amyloid fibrils, techniques used conventionally to analyze protein structure and dynamics have often been hampered. Ultrasonication can induce the monomeric solution of amyloidogenic proteins to form amyloid fibrils. However, ultrasonication can break down prefor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chatani E,Lee YH,Yagi H,Yoshimura Y,Naiki H,Goto Y

更新日期：2009-07-07 00:00:00

abstract：:The Rhizobium common nod gene products NodABC are involved in the synthesis of the core lipochitooligosaccharide (Nod factor) structure, whereas the products of the host-specific nod genes are necessary for diverse structural modifications, which vary in different Rhizobium species. The sulfate group attached to the R...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schultze M,Staehelin C,Röhrig H,John M,Schmidt J,Kondorosi E,Schell J,Kondorosi A

更新日期：1995-03-28 00:00:00

abstract：:Noninvasive prenatal testing using fetal DNA in maternal plasma is an actively researched area. The current generation of tests using massively parallel sequencing is based on counting plasma DNA sequences originating from different genomic regions. In this study, we explored a different approach that is based on the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu SC,Chan KC,Zheng YW,Jiang P,Liao GJ,Sun H,Akolekar R,Leung TY,Go AT,van Vugt JM,Minekawa R,Oudejans CB,Nicolaides KH,Chiu RW,Lo YM

更新日期：2014-06-10 00:00:00

abstract：:The glycosyltransferases of the mammalian Golgi complex must recycle between the stacked cisternae of that organelle to maintain their proper steady-state localization. This trafficking is mediated by COPI-coated vesicles, but how the glycosyltransferases are incorporated into these transport vesicles is poorly unders...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu L,Doray B,Kornfeld S

更新日期：2018-09-04 00:00:00

abstract：:The human renin gene was isolated from a Charon 4A human genomic library and characterized. The gene spans about 11.7 kilobases and consists of 10 exons and 9 introns that map at points that could be variable surface loops of the enzyme. The complete coding regions, the 5'- and 3'-flanking regions, and the exon-intron...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miyazaki H,Fukamizu A,Hirose S,Hayashi T,Hori H,Ohkubo H,Nakanishi S,Murakami K

更新日期：1984-10-01 00:00:00

abstract：:This investigation was undertaken to determine the possible role of growth hormone (GH) in the hormonal regulation of hepatic low density lipoprotein (LDL) receptor expression. Treatment of normal rats with estrogen (ethynylestradiol, 5 mg/kg per day) increased the number of hepatic LDL receptors, and the LDL receptor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rudling M,Norstedt G,Olivecrona H,Reihnér E,Gustafsson JA,Angelin B

更新日期：1992-08-01 00:00:00

abstract：:Serotonin (5-hydroxytryptamine; 0.5 microM and above) stimulated the synthesis of prostacyclin (as measured by radioimmunoassay of 6-ketoprostaglandin F1 alpha) by bovine aortic smooth muscle cells in culture. This effect was structurally specific; a similar response was not elicited by the other indoles (tryptophan, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coughlin SR,Moskowitz MA,Antoniades HN,Levine L

更新日期：1981-11-01 00:00:00

abstract：:In skeletal muscle, dihydropyridine receptors and dihydropyridine-sensitive Ca2+ channels are preferentially localized in the transverse tubular membranes. Starting with an antigenic membrane fraction enriched in rabbit muscle transverse tubules (T-tubules), several monoclonal antibodies were produced by a fusion of s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malouf NN,Coronado R,McMahon D,Meissner G,Gillespie GY

更新日期：1987-07-01 00:00:00

abstract：:In feeding, aphids inject saliva into plant tissues, gaining access to phloem sap and eliciting (and sometimes overcoming) plant responses. We are examining the involvement, in this aphid-plant interaction, of individual aphid proteins and enzymes, as identified in a salivary gland cDNA library. Here, we focus on a sa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mutti NS,Louis J,Pappan LK,Pappan K,Begum K,Chen MS,Park Y,Dittmer N,Marshall J,Reese JC,Reeck GR

更新日期：2008-07-22 00:00:00

abstract：:The karyophilic properties of the HIV-1 nucleoprotein complex facilitate infection of nondividing cells such as macrophages and quiescent T lymphocytes, and allow the in vivo delivery of transgenes by HIV-derived retroviral vectors into terminally differentiated cells such as neurons. Although the viral matrix (MA) an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gallay P,Hope T,Chin D,Trono D

更新日期：1997-09-02 00:00:00

abstract：:Positive-strand RNA viruses are the largest virus class and include many pathogens such as hepatitis C virus and the severe acute respiratory syndrome coronavirus (SARS). Brome mosaic virus (BMV) is a representative positive-strand RNA virus whose RNA replication, gene expression, and encapsidation have been reproduce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kushner DB,Lindenbach BD,Grdzelishvili VZ,Noueiry AO,Paul SM,Ahlquist P

更新日期：2003-12-23 00:00:00

abstract：:Adverse social conditions have been linked to a conserved transcriptional response to adversity (CTRA) in circulating leukocytes that may contribute to social gradients in disease. However, the CNS mechanisms involved remain obscure, in part because CTRA gene-expression profiles often track external social-environment...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mehl MR,Raison CL,Pace TWW,Arevalo JMG,Cole SW

更新日期：2017-11-21 00:00:00

abstract：:Deregulated production of nitric oxide (NO) has been implicated in the development of certain human diseases, including cancer. We sought to assess the damaging potential of NO produced under long-term conditions through the development of a suitable model cell culture system. In this study, we report that when murine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhuang JC,Wogan GN

更新日期：1997-10-28 00:00:00

abstract：:The development of cardiac hypertrophy is mediated, in part, by increase in NADPH oxidase activity and myocardial oxidative stress. The Rho GTPase, Rac, regulates NADPH oxidase activity through interaction with gp91(phox) and p67(phox) (in which "phox" is phagocyte oxidase). However, it is not known which Rac isoform ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Satoh M,Ogita H,Takeshita K,Mukai Y,Kwiatkowski DJ,Liao JK

更新日期：2006-05-09 00:00:00

abstract：:Genetic variation in the peptide-binding groove of the highly polymorphic HLA class I molecules has repeatedly been associated with HIV-1 control and progression to AIDS, accounting for up to 12% of the variation in HIV-1 set point viral load (spVL). This suggests a key role in disease control for HLA presentation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arora J,McLaren PJ,Chaturvedi N,Carrington M,Fellay J,Lenz TL

更新日期：2019-01-15 00:00:00

abstract：:Clostridium difficile is the leading cause of hospital-acquired diarrhea in the United States. The two main virulence factors of C. difficile are the large toxins, TcdA and TcdB, which enter colonic epithelial cells and cause fluid secretion, inflammation, and cell death. Using a gene-trap insertional mutagenesis scre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： LaFrance ME,Farrow MA,Chandrasekaran R,Sheng J,Rubin DH,Lacy DB

更新日期：2015-06-02 00:00:00

abstract：:Natural killer (NK) cells recognize alloantigens on normal cells. One of these alloantigens correlates with homozygosity for a dimorphism of HLA-C at positions 77-80, which is shared by a number of HLA-C alleles. A second allelic alloantigen correlates with homozygosity for the alternative HLA-C dimorphism, which is s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colonna M,Borsellino G,Falco M,Ferrara GB,Strominger JL

更新日期：1993-12-15 00:00:00

abstract：:Crithidia fasciculata cells were treated with a plasmid (pDK96) containing pBR322 sequences, a Leishmania tarentolae maxicircle autonomously replicating sequence, and the bacterial gene for aminoglycoside 3' phosphotransferase I inserted between the yeast alcohol dehydrogenase 1 promotor and terminator sequences. Resi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes DE,Simpson L

更新日期：1986-08-01 00:00:00

abstract：:Genetic studies have revealed thousands of loci predisposing to hundreds of human diseases and traits, revealing important biological pathways and defining novel therapeutic hypotheses. However, the genes discovered to date typically explain less than half of the apparent heritability. Because efforts have largely foc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zuk O,Schaffner SF,Samocha K,Do R,Hechter E,Kathiresan S,Daly MJ,Neale BM,Sunyaev SR,Lander ES

更新日期：2014-01-28 00:00:00

abstract：:NIH 3T3 mouse cells were transfected with plasmids that induce efficient expression of either (i) the Rous sarcoma virus v-src gene, (ii) the chicken c-src gene, or (iii) a recombinant gene combining the 5' portion of c-src with the 3' end of v-src. Focus formation in tissue culture and formation of large colonies in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shalloway D,Coussens PM,Yaciuk P

更新日期：1984-11-01 00:00:00

abstract：:Mutations in the presenilin 1 (PS1) and presenilin 2 (PS2) genes increase the production of the highly amyloidogenic 42-residue form of amyloid beta-protein (Abeta42) in a variety of cell lines and transgenic mice. To elucidate the molecular mechanism of this effect, wild-type (wt) or mutant PS1 and PS2 genes were sta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xia W,Zhang J,Perez R,Koo EH,Selkoe DJ

更新日期：1997-07-22 00:00:00