Evidence for conversion of heteroduplex transforming DNAs to homoduplexes by recipient pneumococcal cells (DNA strand resolution-DNA repair-bacterial transformation-genetic recombination).

abstract：:The effect of combining hormonal treatment consisting of long-acting microcapsules of the agonist [D-Trp6]LH-RH (the D-tryptophan-6 analog of luteinizing hormone-releasing hormone) with the chemotherapeutic agent cyclophosphamide was investigated in the Dunning R-3327H rat prostate cancer model. Microcapsules of [D-Tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schally AV,Redding TW

更新日期：1985-04-01 00:00:00

abstract：:The importance of Gly-93 and Gly-94 in transmembrane segment M1 of the Na+,K+-ATPase for interaction with Na+ and K+ was demonstrated by functional analysis of mutants Gly-93-Ala and Gly-94-Ala. In the crystal structures of the Ca2+-ATPase, the corresponding residues, Asp-59 and Leu-60, are located exactly where M1 be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Einholm AP,Toustrup-Jensen M,Andersen JP,Vilsen B

更新日期：2005-08-09 00:00:00

abstract：:Mitochondria isolated from maize plants with S-type male-sterile cytoplasms are capable of synthesizing four species of RNA at concentrations of actinomycin D that eliminate all DNA-directed RNA synthesis. No RNA synthesis occurs under the same conditions with mitochondria from plants possessing normal (N) cytoplasm o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Finnegan PM,Brown GG

更新日期：1986-07-01 00:00:00

abstract：:Recent cryoEM density maps of Abeta(42) fibrils obtained at low pH revealed two protofilaments winding around a hollow core raising the question if such tubular structures also exist at physiological pH. Based on the cryoEM measurements and on NMR data, we probe amyloid fibril organizations corresponding to the observ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller Y,Ma B,Tsai CJ,Nussinov R

更新日期：2010-08-10 00:00:00

abstract：:The operon model was proposed by Jacob and Monod in 1961 to explain the regulation of enzyme synthesis in bacteria. The model requires two genetic elements: a locus i which directs the synthesis of a repressor, and a locus called the operon which contains an operator gene and associated structural genes. The repressor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zubay G,Lederman M

更新日期：1969-02-01 00:00:00

abstract：:Malignant spreading of cancer cells requires cell surface proteases that cleave the crosslinked collagenous matrix of connective tissues. From correlating the morphologically defined invasiveness of tumor cells with the presence of specific membrane-associated proteases, we have identified a malignant human melanoma c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aoyama A,Chen WT

更新日期：1990-11-01 00:00:00

abstract：:A mitochondrial gene from Saccharomyces cerevisiae encoding a hydrophobic membrane protein, subunit 8 of the F0/F1-type mitochondrial ATPase complex, has been functionally replaced by an artificial nuclear gene specifying an imported version of this protein. The experiments reported here utilized a multicopy expressio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nagley P,Farrell LB,Gearing DP,Nero D,Meltzer S,Devenish RJ

更新日期：1988-04-01 00:00:00

abstract：:Here the first reproductive sterility system for the tephritid fruit fly pest, Anastrepha suspensa, is presented, based on lethality primarily limited to embryos heterozygous for a conditional lethal transgene combination. This tetracycline (Tet)-suppressible system uses a driver construct having the promoter from the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schetelig MF,Handler AM

更新日期：2012-06-12 00:00:00

abstract：:Two-dimensional gel electrophoresis has allowed the detection of proteins characteristic of inner cell mass and trophectoderm in mouse blastocyst. Certain of the proteins characterizing trophectoderm copurify with intermediate filaments from trophectoderm and a trophoblastoma cell line. A monoclonal antibody prepared ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brûlet P,Babinet C,Kemler R,Jacob F

更新日期：1980-07-01 00:00:00

abstract：:Hodgkin disease (HD) is characterized by a small number of malignant Hodgkin and Reed-Sternberg (H/RS) cells among a major population of nonmalignant cells. The analysis of H/RS cells has been hampered by their low frequency and fragility. Here, we describe the isolation of viable H/RS cells from HD affected tissues b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Irsch J,Nitsch S,Hansmann ML,Rajewsky K,Tesch H,Diehl V,Jox A,Küppers R,Radbruch A

更新日期：1998-08-18 00:00:00

abstract：:Cyclophilins are a family of proteins that bind cyclosporin A (CsA) and possess peptidyl-prolyl cis-trans isomerase activity. In addition, they are secreted by activated cells and act in a cytokine-like manner, presumably via signaling through a cell surface cyclophilin receptor. More recently, host-derived cyclophili...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sherry B,Zybarth G,Alfano M,Dubrovsky L,Mitchell R,Rich D,Ulrich P,Bucala R,Cerami A,Bukrinsky M

更新日期：1998-02-17 00:00:00

abstract：:Proalbumins are rare genetic variants of human serum albumin containing a basic propeptide that is not removed during post-transcriptional processing because of a mutation in the site of excision, an Arg-Arg sequence. We have identified the amino acid substitutions in three different types of proalbumins designated Ga...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takahashi N,Takahashi Y,Putnam FW

更新日期：1987-11-01 00:00:00

abstract：:Cyclonic atmospheric vortices of varying intensity, collectively known as low-pressure systems (LPS), travel northwest across central India and produce more than half of the precipitation received by that fertile region and its ∼600 million inhabitants. Yet, future changes in LPS activity are poorly understood, due in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sandeep S,Ajayamohan RS,Boos WR,Sabin TP,Praveen V

更新日期：2018-03-13 00:00:00

abstract：:Establishing a timeline for the evolution of novelties is a common, unifying goal at the intersection of evolutionary and developmental biology. Analyses of gene regulatory networks (GRNs) provide the ability to understand the underlying genetic and developmental mechanisms responsible for the origin of morphological ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thompson JR,Erkenbrack EM,Hinman VF,McCauley BS,Petsios E,Bottjer DJ

更新日期：2017-06-06 00:00:00

abstract：:Salicylic acid (SA) plays an important role in activating various plant defense responses, including expression of the pathogenesis-related (PR) genes and systemic acquired resistance. A critical positive regulator of the SA signaling pathway in Arabidopsis is encoded by the NPR1 gene. However, there is growing eviden...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kachroo P,Shanklin J,Shah J,Whittle EJ,Klessig DF

更新日期：2001-07-31 00:00:00

abstract：:Redistribution of specialized molecules in migrating cells develops asymmetry between two opposite cell poles, the leading edge and the uropod. We show that acquisition of a motile phenotype in T lymphocytes results in the asymmetric redistribution of ganglioside GM3- and GM1-enriched raft domains to the leading edge ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gómez-Móuton C,Abad JL,Mira E,Lacalle RA,Gallardo E,Jiménez-Baranda S,Illa I,Bernad A,Mañes S,Martínez-A C

更新日期：2001-08-14 00:00:00

abstract：:An effective plant alkaloid chemical defense requires a variety of transport processes, but few alkaloid transporters have been characterized at the molecular level. Previously, a gene fragment encoding a putative plasma membrane proton symporter was isolated, because it was coordinately regulated with several nicotin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hildreth SB,Gehman EA,Yang H,Lu RH,Ritesh KC,Harich KC,Yu S,Lin J,Sandoe JL,Okumoto S,Murphy AS,Jelesko JG

更新日期：2011-11-01 00:00:00

abstract：:EF-hand Ca(II) binding sites share a conserved architecture and are prevalent in Ca(II) signaling pathways. The ion binding kinetics of these sites are carefully tuned to provide the physiologically appropriate activation and inactivation time scales. Here we examine kinetic tuning by the side chain at the ninth posit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renner M,Danielson MA,Falke JJ

更新日期：1993-07-15 00:00:00

abstract：:Pyochelin, an endogenous growth promoter that solubilizes ferric iron, has been isolated from Pseudomonas aeruginosa, including clinical strains. The structure of pyochelin has been assigned as 2-(2-o-hydroxyphenyl-2-thiazolin-4-yl)-3-methylthiazolidine-4-carboxylic acid and is of a different type from those previousl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cox CD,Rinehart KL Jr,Moore ML,Cook JC Jr

更新日期：1981-07-01 00:00:00

abstract：:Recent studies show that plant organ positioning may be mediated by localized concentrations of the plant hormone auxin. Auxin patterning in the shoot apical meristem is in turn brought about by the subcellular polar distribution of the putative auxin efflux mediator, PIN1. However, the question of what signals determ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jönsson H,Heisler MG,Shapiro BE,Meyerowitz EM,Mjolsness E

更新日期：2006-01-31 00:00:00

abstract：:Larval fishes suffer prodigious mortality rates, eliminating 99% of the brood within a few days after first feeding. Hjort (1914) famously attributed this "critical period" of low survival to the larvae's inability to obtain sufficient food [Hjort (1914) Rapp P-v Réun Cons Int Explor Mer 20:1-228]. However, the cause ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： China V,Holzman R

更新日期：2014-06-03 00:00:00

abstract：:Donor DNA molecules carrying Tn1 or Tn3 deletion mutants do not need to replicate in order to participate in replicon fusion recombination events during which the Tn1/Tn3 element is duplicated. We have assayed Tn1 delta Ap-mediated replicon fusion events involving plasmid R388 and the bacteriophage lambda-derived plas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Muster CJ,Shapiro JA,MacHattie LA

更新日期：1983-04-01 00:00:00

abstract：:Allozyme, morphological, and PCR-generated mtDNA markers were used to analyze hybrid parentage and introgression between the neighboringly sympatric crustaceans Daphnia galeata mendotae and Daphnia rosea. mtDNA analysis of D. galeata mendotae from 15 lakes and of D. rosea from 8 lakes revealed species-specific fragmen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taylor DJ,Hebert PD

更新日期：1993-08-01 00:00:00

abstract：:Previous studies have defined transcriptional control elements, in addition to the promoters, that both lie near individual human beta-globin locus genes and have been implicated in their differential stage-specific regulation during development (i.e., are believed to directly participate in hemoglobin switching). We ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu Q,Bungert J,Engel JD

更新日期：1997-01-07 00:00:00

abstract：:Type I interferons (IFNs) are helical cytokines that have diverse biological activities despite the fact that they appear to interact with the same receptor system. To achieve a better understanding of the structural basis for the different activities of alpha and beta IFNs, we have determined the crystal structure of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karpusas M,Nolte M,Benton CB,Meier W,Lipscomb WN,Goelz S

更新日期：1997-10-28 00:00:00

abstract：:We report here that during a permanent cardiac arrest, rodent brain tissue is "physiologically" preserved in situ in a particular quiescent state. This state is characterized by the absence of electrical activity and by a critical period of 5-6 hr during which brain tissue can be reactivated upon restoration of a simp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Charpak S,Audinat E

更新日期：1998-04-14 00:00:00

abstract：:We have examined the accumulation of newly synthesized nuclear proteins into nuclei during DNA repair synthesis in confluent WI-38 human diploid fibroblasts damaged with ultraviolet radiation or N-acetoxy-2-acetylaminofluroene. In contrast to a marked stimulation of DNA repair synthesis, stimulation of amino acid inco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stein GS,Park WD,Stein JL,Lieberman MW

更新日期：1976-05-01 00:00:00

abstract：:The presence of nerve growth factor (NGF) mRNA and protein in the rat central nervous system is documented. Blot-hybridization analysis showed an abundance of NGF mRNA in the hippocampus, cerebral cortex, and olfactory bulb. Enzyme immunoassay confirmed significant levels of a NGF-like protein in the hippocampus and c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Whittemore SR,Ebendal T,Lärkfors L,Olson L,Seiger A,Strömberg I,Persson H

更新日期：1986-02-01 00:00:00

abstract：:Measurements of auditory sensitivity in terms of the cochlear potentials were made in young specimens of three species of crocodilians, Caiman crocodilus and Alligator mississipiensis of the subfamily Alligatorinae and Crocodylus acutus of the subfamily Crocodylinae. These species show considerable similarity in their...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wever EG

更新日期：1971-07-01 00:00:00

abstract：:Isolated yeast mitochondria, which synthesize identifiable polypeptides identical to those made in vivo, have been used in an invitro system to study cytoplasmic control of mitochondrial protein synthesis. It has been found that protein synthesis in isolated mitochondria is dependent on an endogenous pool of cytoplasm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poyton RO,Kavanagh J

更新日期：1976-11-01 00:00:00