Tobacco nicotine uptake permease (NUP1) affects alkaloid metabolism.

Abstract:

:An effective plant alkaloid chemical defense requires a variety of transport processes, but few alkaloid transporters have been characterized at the molecular level. Previously, a gene fragment encoding a putative plasma membrane proton symporter was isolated, because it was coordinately regulated with several nicotine biosynthetic genes. Here, we show that this gene fragment corresponds to a Nicotiana tabacum gene encoding a nicotine uptake permease (NUP1). NUP1 belongs to a plant-specific class of purine uptake permease-like transporters that originated after the bryophytes but before or within the lycophytes. NUP1 expressed in yeast cells preferentially transported nicotine relative to other pyridine alkaloids, tropane alkaloids, kinetin, and adenine. NUP1-GFP primarily localized to the plasma membrane of tobacco Bright Yellow-2 protoplasts. WT NUP1 transcripts accumulated to high levels in the roots, particularly in root tips. NUP1-RNAi hairy roots had reduced NUP1 mRNA accumulation levels, reduced total nicotine levels, and increased nicotine accumulation in the hairy root culture media. Regenerated NUP1-RNAi plants showed reduced foliar and root nicotine levels as well as increased seedling root elongation rates. Thus, NUP1 affected nicotine metabolism, localization, and root growth.

authors

Hildreth SB,Gehman EA,Yang H,Lu RH,Ritesh KC,Harich KC,Yu S,Lin J,Sandoe JL,Okumoto S,Murphy AS,Jelesko JG

doi

10.1073/pnas.1108620108

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

18179-84

issue

44

eissn

0027-8424

issn

1091-6490

pii

1108620108

journal_volume

108

pub_type

杂志文章
  • Perturbation of the T cell repertoire in rheumatoid arthritis.

    abstract::Aberrations in the T cell repertoire with the emergence of oligoclonal populations have been described in patients with rheumatoid arthritis (RA). However, the extent of the repertoire perturbations as well as the underlying mechanisms are not known. We now have examined the diversity of the peripheral CD4 T cell repe...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.95.24.14447

    authors: Wagner UG,Koetz K,Weyand CM,Goronzy JJ

    更新日期:1998-11-24 00:00:00

  • Carbon monoxide binding kinetics in "capped" porphyrin compounds.

    abstract::The rate constants for CO binding to the five-coordinate ferrous iron complexes of 5,10,15,20-[pyromellitoyl(tetrakis-o-oxyoxyphenyl)]porphyrin and 5,10,15,20-[pyromellitoyl(tetrakis-o-oxypropoxyphenyl)]porphyrin have been measured and compared with the corresponding rate constants for other hemes and hemoproteins. Th...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.79.18.5742

    authors: Rose EJ,Venkatasubramanian PN,Swartz JC,Jones RD,Basolo F,Hoffman BM

    更新日期:1982-09-01 00:00:00

  • Von Willebrand protein binds to extracellular matrices independently of collagen.

    abstract::Von Willebrand protein is present in the extracellular matrix of endothelial cells where it codistributes with fibronectin and types IV and V collagen. Bacterial collagenase digestion of endothelial cells removed fibrillar collagen, but the pattern of fibronectin and of von Willebrand protein remained undisturbed. Exo...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.81.2.471

    authors: Wagner DD,Urban-Pickering M,Marder VJ

    更新日期:1984-01-01 00:00:00

  • Local kinetics of morphogen gradients.

    abstract::Some aspects of pattern formation in developing embryos can be described by nonlinear reaction-diffusion equations. An important class of these models accounts for diffusion and degradation of a locally produced single chemical species. At long times, solutions of such models approach a steady state in which the conce...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1019245108

    authors: Gordon PV,Sample C,Berezhkovskii AM,Muratov CB,Shvartsman SY

    更新日期:2011-04-12 00:00:00

  • Antibody-dependent enhancement of influenza disease promoted by increase in hemagglutinin stem flexibility and virus fusion kinetics.

    abstract::Several next-generation (universal) influenza vaccines and broadly neutralizing antibodies (bNAbs) are in clinical development. Some of these mediate inhibitions of virus replication at the postentry stage or use Fc-dependent mechanisms. Nonneutralizing antibodies have the potential to mediate enhancement of viral inf...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1821317116

    authors: Winarski KL,Tang J,Klenow L,Lee J,Coyle EM,Manischewitz J,Turner HL,Takeda K,Ward AB,Golding H,Khurana S

    更新日期:2019-07-23 00:00:00

  • Modification of host dendritic cells by microchimerism-derived extracellular vesicles generates split tolerance.

    abstract::Maternal microchimerism (MMc) has been associated with development of allospecific transplant tolerance, antitumor immunity, and cross-generational reproductive fitness, but its mode of action is unknown. We found in a murine model that MMc caused exposure to the noninherited maternal antigens in all offspring, but in...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1618364114

    authors: Bracamonte-Baran W,Florentin J,Zhou Y,Jankowska-Gan E,Haynes WJ,Zhong W,Brennan TV,Dutta P,Claas FH,van Rood JJ,Burlingham WJ

    更新日期:2017-01-31 00:00:00

  • IKKβ is an IRF5 kinase that instigates inflammation.

    abstract::The transcription factor interferon regulatory factor 5 (IRF5) is essential for the induction of inflammatory cytokines, but the mechanism by which IRF5 is activated is not well understood. Here we present evidence that the kinase IKKβ phosphorylates and activates IRF5 in response to stimulation in several inflammator...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1418516111

    authors: Ren J,Chen X,Chen ZJ

    更新日期:2014-12-09 00:00:00

  • DNase I hypersensitive sites within the inducible qa gene cluster of Neurospora crassa.

    abstract::DNase I hypersensitive regions were mapped within the 17.3-kilobase qa (quinic acid) gene cluster of Neurospora crassa. The 5'-flanking regions of the five qa structural genes and the two qa regulatory genes each contain DNase I hypersensitive sites under noninducing conditions and generally exhibit increases in DNase...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.17.6533

    authors: Baum JA,Giles NH

    更新日期:1986-09-01 00:00:00

  • Inducible nitric oxide synthase expression inhibition by adenovirus E1A.

    abstract::Nitric oxide (NO) is an antiviral effector of the innate immune system. Viruses that can interfere with NO synthesis may be able to replicate more rapidly than viruses that cannot limit NO synthesis. We show that the adenovirus E1A protein inhibits NO production by decreasing expression of the inducible NO synthase (N...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1337185100

    authors: Cao W,Bao C,Lowenstein CJ

    更新日期:2003-06-24 00:00:00

  • Fighting fruit flies: a model system for the study of aggression.

    abstract::Despite the importance of aggression in the behavioral repertoire of most animals, relatively little is known of its proximate causation and control. To take advantage of modern methods of genetic analysis for studying this complex behavior, we have developed a quantitative framework for studying aggression in common ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.082102599

    authors: Chen S,Lee AY,Bowens NM,Huber R,Kravitz EA

    更新日期:2002-04-16 00:00:00

  • Superoxo, mu-peroxo, and mu-oxo complexes from heme/O2 and heme-Cu/O2 reactivity: copper ligand influences in cytochrome c oxidase models.

    abstract::The O(2)-reaction chemistry of 1:1 mixtures of (F(8))Fe(II) (1; F(8) = tetrakis(2,6-diflurorophenyl)porphyrinate) and [(L(Me(2))N)Cu(I)](+) (2; L(Me(2))N = N,N-bis(2-[2-(N',N'-4-dimethylamino)pyridyl]ethyl)methylamine) is described, to model aspects of the chemistry occurring in cytochrome c oxidase. Spectroscopic inv...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0737180100

    authors: Kim E,Helton ME,Wasser IM,Karlin KD,Lu S,Huang HW,Moenne-Loccoz P,Incarvito CD,Rheingold AL,Honecker M,Kaderli S,Zuberbuhler AD

    更新日期:2003-04-01 00:00:00

  • Target-effector interaction in the natural killer cell system: isolation of target structures.

    abstract::A sensitive target binding assay has recently been shown to detect natural killer (NK) cells in the mouse. Preincubation of NK cells with detergent-solubilized cell-surface proteins of YAC lymphoma cells prevented subsequent binding to intact YAC targets. The NK target structures (NK-TS) consisted of three molecular s...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.3.1405

    authors: Roder JC,Rosén A,Fenyö EM,Troy FA

    更新日期:1979-03-01 00:00:00

  • Immunoregulatory activity of the T-cell receptor alpha chain demonstrated by retroviral gene transfer.

    abstract::We have previously described an antigen-specific I-Ad-restricted T-cell hybridoma, A1.1, that constitutively releases an antigen-specific immunoregulatory activity into supernatants. Using retrovirally mediated gene transfer, we have found that transfer of the T-cell receptor alpha chain (TCR alpha) gene from A1.1 to ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.19.8475

    authors: Green DR,Bissonnette R,Zheng HG,Onda T,Echeverri F,Mogil RJ,Steele JK,Voralia M,Fotedar A

    更新日期:1991-10-01 00:00:00

  • A dual role for the kinase-like domain of the tyrosine kinase Tyk2 in interferon-alpha signaling.

    abstract::Tyrosine kinases of the Janus kinase family initiate cellular responses through their association with receptors for alpha-helical cytokines. In addition to a tyrosine kinase domain, these enzymes possess a kinase-like (KL) domain, whose function remains elusive. To investigate the role of the KL domain of Tyk2 in int...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.160130297

    authors: Yeh TC,Dondi E,Uze G,Pellegrini S

    更新日期:2000-08-01 00:00:00

  • XMAP215 polymerase activity is built by combining multiple tubulin-binding TOG domains and a basic lattice-binding region.

    abstract::XMAP215/Dis1 family proteins positively regulate microtubule growth. Repeats at their N termini, called TOG domains, are important for this function. While TOG domains directly bind tubulin dimers, it is unclear how this interaction translates to polymerase activity. Understanding the functional roles of TOG domains i...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1016498108

    authors: Widlund PO,Stear JH,Pozniakovsky A,Zanic M,Reber S,Brouhard GJ,Hyman AA,Howard J

    更新日期:2011-02-15 00:00:00

  • Expression of enzymatically active reverse transcriptase in Escherichia coli.

    abstract::Reverse transcriptase of murine retroviruses is a monomeric protein of approximately 80,000 daltons, which is encoded by the central portion of the viral pol gene. To prepare large quantities of the enzyme, we have constructed gene fusions between the trpE gene and portions of the pol gene of Moloney murine leukemia v...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.15.4944

    authors: Tanese N,Roth M,Goff SP

    更新日期:1985-08-01 00:00:00

  • Metaxin, a gene contiguous to both thrombospondin 3 and glucocerebrosidase, is required for embryonic development in the mouse: implications for Gaucher disease.

    abstract::We have identified a murine gene, metaxin, that spans the 6-kb interval separating the glucocerebrosidase gene (GC) from the thrombospondin 3 gene on chromosome 3E3-F1. Metaxin and GC are transcribed convergently; their major polyadenylylation sites are only 431 bp apart. On the other hand, metaxin and the thrombospon...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.10.4547

    authors: Bornstein P,McKinney CE,LaMarca ME,Winfield S,Shingu T,Devarayalu S,Vos HL,Ginns EI

    更新日期:1995-05-09 00:00:00

  • Adaptive diversification of growth allometry in the plant Arabidopsis thaliana.

    abstract::Seed plants vary tremendously in size and morphology; however, variation and covariation in plant traits may be governed, at least in part, by universal biophysical laws and biological constants. Metabolic scaling theory (MST) posits that whole-organismal metabolism and growth rate are under stabilizing selection that...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1709141115

    authors: Vasseur F,Exposito-Alonso M,Ayala-Garay OJ,Wang G,Enquist BJ,Vile D,Violle C,Weigel D

    更新日期:2018-03-27 00:00:00

  • Synthesis of mannosylglucosaminylinositol phospholipids in normal but not paroxysmal nocturnal hemoglobinuria cells.

    abstract::To identify mannosyl (Man)-containing intermediates of the human glycoinositol phospholipid (GPI) anchor pathway and examine their expression in paroxysmal nocturnal hemoglobinuria (PNH), mannolipid products deriving from in vitro guanosine diphosphate [3H]Man labeling of HeLa cell microsomes were characterized. The d...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.13.6025

    authors: Hirose S,Ravi L,Prince GM,Rosenfeld MG,Silber R,Andresen SW,Hazra SV,Medof ME

    更新日期:1992-07-01 00:00:00

  • Inversin relays Frizzled-8 signals to promote proximal pronephros development.

    abstract::Mutations of inversin cause type II nephronophthisis, an infantile autosomal recessive disease characterized by cystic kidney disease and developmental defects. Inversin regulates Wnt signaling and is required for convergent extension movements during early embryogenesis. We now show that Inversin is essential for Xen...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1013070107

    authors: Lienkamp S,Ganner A,Boehlke C,Schmidt T,Arnold SJ,Schäfer T,Romaker D,Schuler J,Hoff S,Powelske C,Eifler A,Krönig C,Bullerkotte A,Nitschke R,Kuehn EW,Kim E,Burkhardt H,Brox T,Ronneberger O,Gloy J,Walz G

    更新日期:2010-11-23 00:00:00

  • The scaling of frontal cortex in primates and carnivores.

    abstract::Size has a profound effect on the structure of the brain. Many brain structures scale allometrically, that is, their relative size changes systematically as a function of brain size. Here we use independent contrasts analysis to examine the scaling of frontal cortex in 43 species of mammals including 25 primates and 1...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0305760101

    authors: Bush EC,Allman JM

    更新日期:2004-03-16 00:00:00

  • Multiple receptors for HLA-G on human natural killer cells.

    abstract::HLA-G is the putative natural killer (NK) cell inhibitory ligand expressed on the extravillous cytotrophoblast of the human placenta. Killing of the class I negative human B cell line 721.221 by NK cells is inhibited by the expression of HLA-G. This inhibition is dependent on a high level of HLA-G expression. In the p...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.94.26.14666

    authors: Mandelboim O,Pazmany L,Davis DM,Valés-Gómez M,Reyburn HT,Rybalov B,Strominger JL

    更新日期:1997-12-23 00:00:00

  • Methyl-compound use and slow growth characterize microbial life in 2-km-deep subseafloor coal and shale beds.

    abstract::The past decade of scientific ocean drilling has revealed seemingly ubiquitous, slow-growing microbial life within a range of deep biosphere habitats. Integrated Ocean Drilling Program Expedition 337 expanded these studies by successfully coring Miocene-aged coal beds 2 km below the seafloor hypothesized to be "hot sp...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1707525114

    authors: Trembath-Reichert E,Morono Y,Ijiri A,Hoshino T,Dawson KS,Inagaki F,Orphan VJ

    更新日期:2017-10-31 00:00:00

  • Switch from Myc/Max to Mad1/Max binding and decrease in histone acetylation at the telomerase reverse transcriptase promoter during differentiation of HL60 cells.

    abstract::Recent evidence suggests that the Myc and Mad1 proteins are implicated in the regulation of the gene encoding the human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase. We have analyzed the in vivo interaction between endogenous c-Myc and Mad1 proteins and the hTERT promoter in HL60 cells...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.071043198

    authors: Xu D,Popov N,Hou M,Wang Q,Björkholm M,Gruber A,Menkel AR,Henriksson M

    更新日期:2001-03-27 00:00:00

  • CRYPTOCHROME mediates behavioral executive choice in response to UV light.

    abstract::Drosophila melanogaster CRYPTOCHROME (CRY) mediates behavioral and electrophysiological responses to blue light coded by circadian and arousal neurons. However, spectroscopic and biochemical assays of heterologously expressed CRY suggest that CRY may mediate functional responses to UV-A (ultraviolet A) light as well. ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1607989114

    authors: Baik LS,Fogle KJ,Roberts L,Galschiodt AM,Chevez JA,Recinos Y,Nguy V,Holmes TC

    更新日期:2017-01-24 00:00:00

  • Aquaporin-4 square array assembly: opposing actions of M1 and M23 isoforms.

    abstract::Osmotic homeostasis in the brain involves movement of water through aquaporin-4 (AQP4) membrane channels. Perivascular astrocyte end-feet contain distinctive orthogonal lattices (square arrays) assembled from 4- to 6-nm intramembrane particles (IMPs) corresponding to individual AQP4 tetramers. Two isoforms of AQP4 res...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2235843100

    authors: Furman CS,Gorelick-Feldman DA,Davidson KG,Yasumura T,Neely JD,Agre P,Rash JE

    更新日期:2003-11-11 00:00:00

  • Insulin stimulates glyceraldehyde-3-phosphate dehydrogenase gene expression through cis-acting DNA sequences.

    abstract::Glyceraldehyde-3-phosphate dehydrogenase [GAPDH; D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating), EC 1.2.1.12] mRNA levels are induced by physiologic concentrations of insulin in cultured 3T3-F442A adipocyte and H35 hepatoma cell lines. To examine the mechanism by which insulin regulates GAPDH mRNA ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.14.5092

    authors: Alexander MC,Lomanto M,Nasrin N,Ramaika C

    更新日期:1988-07-01 00:00:00

  • Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.

    abstract::Denaturing gradient gel electrophoresis (DGGE) can be used to distinguish two DNA molecules that differ by as little as a single-base substitution. This method detects approximately 50% of all possible single-base changes in DNA fragments ranging from 50 to approximately 1000 base pairs. To increase the number of sing...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.1.232

    authors: Sheffield VC,Cox DR,Lerman LS,Myers RM

    更新日期:1989-01-01 00:00:00

  • Extracellular K+ specifically modulates a rat brain K+ channel.

    abstract::Extracellular potassium concentration is actively maintained within narrow limits in all higher organisms. Slight variations in extracellular potassium levels can induce major alterations of essential physiological functions in excitable tissues. Here we describe that superfusion of cultured rat hippocampal neurones w...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.6.2466

    authors: Pardo LA,Heinemann SH,Terlau H,Ludewig U,Lorra C,Pongs O,Stühmer W

    更新日期:1992-03-15 00:00:00

  • Attenuation of virulence in Mycobacterium tuberculosis expressing a constitutively active iron repressor.

    abstract::Iron is an essential nutrient for the survival of most organisms and has played a central role in the virulence of many infectious disease pathogens. Mycobacterial IdeR is an iron-dependent repressor that shows 80% identity in the functional domains with its corynebacterial homologue, DtxR (diphtheria toxin repressor)...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.22.12844

    authors: Manabe YC,Saviola BJ,Sun L,Murphy JR,Bishai WR

    更新日期:1999-10-26 00:00:00