当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Kinetic control of Ca(II) signaling: tuning the ion dissociation rates of EF-hand Ca(II) binding sites.

Kinetic control of Ca(II) signaling: tuning the ion dissociation rates of EF-hand Ca(II) binding sites.

Abstract：

:EF-hand Ca(II) binding sites share a conserved architecture and are prevalent in Ca(II) signaling pathways. The ion binding kinetics of these sites are carefully tuned to provide the physiologically appropriate activation and inactivation time scales. Here we examine kinetic tuning by the side chain at the ninth position of the EF-loop. A model is proposed in which both the size and charge of the side chain contribute to kinetic tuning. To test this model, the ninth loop position of the EF-hand-like site in the Escherichia coli D-galactose binding protein has been engineered and the Tb(III) dissociation kinetics of the resulting sites have been analyzed. Substitutions at this position are observed to generate up to 10(4)-fold changes in Tb(III) dissociation rates, with little effect on Tb(III) affinity. Furthermore, the observed pattern of rate changes confirm the model's predictions; long side chains at the ninth loop position yield slow dissociation kinetics as predicted for a steric block, whereas acidic side chains yield slow dissociation kinetics as expected for an electrostatic barrier.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Renner M,Danielson MA,Falke JJ

doi

10.1073/pnas.90.14.6493

subject

Has Abstract

pub_date

1993-07-15 00:00:00

pages

6493-7

issue

14

eissn

0027-8424

issn

1091-6490

journal_volume

90

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein.

    abstract：:Seasonal epidemics and periodic worldwide pandemics caused by influenza A viruses are of continuous concern. The viral nonstructural (NS1) protein is a multifunctional virulence factor that antagonizes several host innate immune defenses during infection. NS1 also directly stimulates class IA phosphoinositide 3-kinase...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0910715107

    authors： Hale BG,Kerry PS,Jackson D,Precious BL,Gray A,Killip MJ,Randall RE,Russell RJ

    更新日期：2010-02-02 00:00:00

  • The chemokine SDF-1/CXCL12 modulates the firing pattern of vasopressin neurons and counteracts induced vasopressin release through CXCR4.

    abstract：:Chemokines play a key role in inflammation. They are expressed not only in neuroinflammatory conditions, but also constitutively by different cell types, including neurons in the normal brain, suggesting that they may act as modulators of neuronal functions. Here, we investigated a possible neuroendocrine role of the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0602620103

    authors： Callewaere C,Banisadr G,Desarménien MG,Mechighel P,Kitabgi P,Rostène WH,Mélik Parsadaniantz S

    更新日期：2006-05-23 00:00:00

  • Role of DNA polymerase alpha and DNA primase in simian virus 40 DNA replication in vitro.

    abstract：:The role of DNA polymerase alpha (pol alpha) and DNA primase has been investigated in the simian virus 40 (SV40) DNA replication system in vitro. Removal of pol alpha and primase activities from crude extracts of HeLa cells or monkey cells by use of an anti-pol alpha immunoaffinity column resulted in the loss of repli...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.9.2869

    authors： Murakami Y,Wobbe CR,Weissbach L,Dean FB,Hurwitz J

    更新日期：1986-05-01 00:00:00

  • Formation of IgE-binding factors by T cells of patients infected with human immunodeficiency virus type 1.

    abstract：:Peripheral blood mononuclear cells of 10 out of 26 patients with human immunodeficiency virus type 1 (HIV-1) released IgE-binding factors, as determined by two independent assays. The formation of the factors by the mononuclear cells was enhanced by incubation of the cells with homologous IgE. In the presence of IgE, ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.23.9214

    authors： Carini C,Margolick J,Yodoi J,Ishizaka K

    更新日期：1988-12-01 00:00:00

  • Molecular dynamics analysis of a buckyball-antibody complex.

    abstract：:This is a multinanosecond molecular dynamics study of a bio-nano complex formed by a carbon nanoparticle, a buckyball C(60), and a biological molecule, an antibody, with high binding affinity and specificity. In the simulation, the ball is completely desolvated by the binding site of the antibody by means of a nearly ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.022532599

    authors： Noon WH,Kong Y,Ma J

    更新日期：2002-04-30 00:00:00

  • Activation of the Keap1/Nrf2 pathway for neuroprotection by electrophilic [correction of electrophillic] phase II inducers.

    abstract：:Electrophilic neurite outgrowth-promoting prostaglandin (NEPP) compounds protect neurons from oxidative insults. At least part of the neuroprotective action of NEPPs lies in induction of hemeoxygenase-1 (HO-1), which, along with other phase II enzymes, serve as a defense system against oxidative stress. Here, we found...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0505723102

    authors： Satoh T,Okamoto SI,Cui J,Watanabe Y,Furuta K,Suzuki M,Tohyama K,Lipton SA

    更新日期：2006-01-17 00:00:00

  • RhoGDIgamma: a GDP-dissociation inhibitor for Rho proteins with preferential expression in brain and pancreas.

    abstract：:GDP-dissociation inhibitors (GDIs) play a primary role in modulating the activation of GTPases and may also be critical for the cellular compartmentalization of GTPases. RhoGDI and GDI/D4 are two currently known GDIs for the Rho-subfamily of GTPases. Using their cDNAs to screen a human brain cDNA library under low str...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.9.4279

    authors： Adra CN,Manor D,Ko JL,Zhu S,Horiuchi T,Van Aelst L,Cerione RA,Lim B

    更新日期：1997-04-29 00:00:00

  • Reduced amounts of cartilage collagen fibrils and growth plate anomalies in transgenic mice harboring a glycine-to-cysteine mutation in the mouse type II procollagen alpha 1-chain gene.

    abstract：:We have generated transgenic mice harboring a glycine-to-cysteine mutation in residue 85 of the triple helical domain of mouse type II collagen. The offspring of different founders displayed a phenotype of severe chondrodysplasia characterized by short limbs and trunk, cranio-facial deformities, and cleft palate. The ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.21.9648

    authors： Garofalo S,Vuorio E,Metsaranta M,Rosati R,Toman D,Vaughan J,Lozano G,Mayne R,Ellard J,Horton W

    更新日期：1991-11-01 00:00:00

  • Strategy for reversing resistance to a single anticancer agent in human prostate and pancreatic carcinomas.

    abstract：:Effective therapies for most solid cancers, especially those that have progressed to metastasis, remain elusive because of inherent and acquired resistance of tumor cells to conventional treatments. Additionally, the effective therapeutic window for many protocols can be very narrow, frequently resulting in toxicity. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0700042104

    authors： Lebedeva IV,Washington I,Sarkar D,Clark JA,Fine RL,Dent P,Curiel DT,Turro NJ,Fisher PB

    更新日期：2007-02-27 00:00:00

  • Functional organization of the cat's visual cortex after prenatal interruption of binocular interactions.

    abstract：:The functional consequences of interrupting in utero binocular interactions were studied by recording from single cells in area 17 of adult cats that had one eye removed at least 2 wk before birth. In these animals all cortical neurons could be driven by the remaining eye, and in tangential microelectrode penetrations...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.11.3901

    authors： Shook BL,Maffei L,Chalupa LM

    更新日期：1985-06-01 00:00:00

  • Computer simulations of protein functions: searching for the molecular origin of the replication fidelity of DNA polymerases.

    abstract：:The use of computers to simulate the functions of complex biological macromolecules is essential to achieve a microscopic description of biological processes and to model and interpret experimental data. Here we apply theoretical computational approaches to investigate the fidelity of T7 DNA polymerase, divided into d...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0408173102

    authors： Florián J,Goodman MF,Warshel A

    更新日期：2005-05-10 00:00:00

  • Biomimetic organization: Octapeptide self-assembly into nanotubes of viral capsid-like dimension.

    abstract：:The controlled self-assembly of complex molecules into well defined hierarchical structures is a promising route for fabricating nanostructures. These nanoscale structures can be realized by naturally occurring proteins such as tobacco mosaic virus, capsid proteins, tubulin, actin, etc. Here, we report a simple altern...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1730609100

    authors： Valéry C,Paternostre M,Robert B,Gulik-Krzywicki T,Narayanan T,Dedieu JC,Keller G,Torres ML,Cherif-Cheikh R,Calvo P,Artzner F

    更新日期：2003-09-02 00:00:00

  • RNA from an immediate early region of the type 1 herpes simplex virus genome is present in the trigeminal ganglia of latently infected mice.

    abstract：:Transcription of the type 1 herpes simplex virus (HSV-1) genome in trigeminal ganglia of latently infected mice was studied using in situ hybridization. Probes representative of each temporal gene class were used to determine the regions of the genome that encode the transcripts present in latently infected cells. Pro...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.10.3204

    authors： Deatly AM,Spivack JG,Lavi E,Fraser NW

    更新日期：1987-05-01 00:00:00

  • Functions of the DNA damage response pathway target Ho endonuclease of yeast for degradation via the ubiquitin-26S proteasome system.

    abstract：:Ho endonuclease of Saccharomyces cerevisiae is a homing endonuclease that makes a site-specific double-strand break in the MAT gene in late G(1). Here we show that Ho is rapidly degraded via the ubiquitin-26S proteasome system through two ubiquitin-conjugating enzymes UBC2(Rad6) and UBC3(Cdc34). UBC2(Rad6) is complexe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.18.10077

    authors： Kaplun L,Ivantsiv Y,Kornitzer D,Raveh D

    更新日期：2000-08-29 00:00:00

  • Biosynthesis of a D-amino acid in peptide linkage by an enzyme from frog skin secretions.

    abstract：:d-amino acids are present in some peptides from amphibian skin. These residues are derived from the corresponding L-amino acids present in the respective precursors. From skin secretions of Bombinae, we have isolated an enzyme that catalyzes the isomerization of an L-Ile in position 2 of a model peptide to D-allo-Ile....

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0500789102

    authors： Jilek A,Mollay C,Tippelt C,Grassi J,Mignogna G,Müllegger J,Sander V,Fehrer C,Barra D,Kreil G

    更新日期：2005-03-22 00:00:00

  • Foreign glycoproteins expressed from recombinant vesicular stomatitis viruses are incorporated efficiently into virus particles.

    abstract：:In a previous study we demonstrated that vesicular stomatitis virus (VSV) can be used as a vector to express a soluble protein in mammalian cells. Here we have generated VSV recombinants that express four different membrane proteins: the cellular CD4 protein, a CD4-G hybrid protein containing the ectodomain of CD4 and...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.21.11359

    authors： Schnell MJ,Buonocore L,Kretzschmar E,Johnson E,Rose JK

    更新日期：1996-10-15 00:00:00

  • Global discovery of colonization determinants in the squid symbiont Vibrio fischeri.

    abstract：:Animal epithelial tissue becomes reproducibly colonized by specific environmental bacteria. The bacteria (microbiota) perform critical functions for the host's tissue development, immune system development, and nutrition; yet the processes by which bacterial diversity in the environment is selected to assemble the cor...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1415957111

    authors： Brooks JF 2nd,Gyllborg MC,Cronin DC,Quillin SJ,Mallama CA,Foxall R,Whistler C,Goodman AL,Mandel MJ

    更新日期：2014-12-02 00:00:00

  • Immunological function of HLA-C antigens in HLA-Cw3 transgenic mice.

    abstract：:The human major histocompatibility complex encodes three classical class I antigens, HLA-A, -B, and -C. Of these HLA-A and -B act as strong transplantation antigens and as restriction molecules for recognition of foreign antigen by cytotoxic T lymphocytes. In contrast, little is known about HLA-C and it is not clear w...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.15.5664

    authors： Dill O,Kievits F,Koch S,Ivanyi P,Hämmerling GJ

    更新日期：1988-08-01 00:00:00

  • Peroxisomal D-hydroxyacyl-CoA dehydrogenase deficiency: resolution of the enzyme defect and its molecular basis in bifunctional protein deficiency.

    abstract：:Peroxisomes play an essential role in a number of different metabolic pathways, including the beta-oxidation of a distinct set of fatty acids and fatty acid derivatives. The importance of the peroxisomal beta-oxidation system in humans is made apparent by the existence of a group of inherited diseases in which peroxis...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.5.2128

    authors： van Grunsven EG,van Berkel E,Ijlst L,Vreken P,de Klerk JB,Adamski J,Lemonde H,Clayton PT,Cuebas DA,Wanders RJ

    更新日期：1998-03-03 00:00:00

  • Human POT1 disrupts telomeric G-quadruplexes allowing telomerase extension in vitro.

    abstract：:The POT1 (protection of telomeres 1) protein binds the ssDNA overhangs at the ends of chromosomes in diverse eukaryotes. POT1 is essential for chromosome end-protection, as best demonstrated in fission yeast. In human cells, hPOT1 is also involved in telomere-length regulation. We now show that telomeric oligonucleoti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0504744102

    authors： Zaug AJ,Podell ER,Cech TR

    更新日期：2005-08-02 00:00:00

  • Training and search methods for speech recognition.

    abstract：:Speech recognition involves three processes: extraction of acoustic indices from the speech signal, estimation of the probability that the observed index string was caused by a hypothesized utterance segment, and determination of the recognized utterance via a search among hypothesized alternatives. This paper is not ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.22.9964

    authors： Jelinek F

    更新日期：1995-10-24 00:00:00

  • Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.

    abstract：:Estrogen plays a critical role in many physiological processes and exerts profound effects on behavior by regulating neuronal excitability. While estrogen has been established to exert effects on dendritic morphology and excitatory neurotransmission its role in regulating neuronal inhibition is poorly understood. Fast...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1705075114

    authors： Mukherjee J,Cardarelli RA,Cantaut-Belarif Y,Deeb TZ,Srivastava DP,Tyagarajan SK,Pangalos MN,Triller A,Maguire J,Brandon NJ,Moss SJ

    更新日期：2017-10-31 00:00:00

  • Ancient polymorphisms and divergence hitchhiking contribute to genomic islands of divergence within a poplar species complex.

    abstract：:How genome divergence eventually leads to speciation is a topic of prime evolutionary interest. Genomic islands of elevated divergence are frequently reported between diverging lineages, and their size is expected to increase with time and gene flow under the speciation-with-gene-flow model. However, such islands can ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1713288114

    authors： Ma T,Wang K,Hu Q,Xi Z,Wan D,Wang Q,Feng J,Jiang D,Ahani H,Abbott RJ,Lascoux M,Nevo E,Liu J

    更新日期：2018-01-09 00:00:00

  • Erralpha and Gabpa/b specify PGC-1alpha-dependent oxidative phosphorylation gene expression that is altered in diabetic muscle.

    abstract：:Recent studies have shown that genes involved in oxidative phosphorylation (OXPHOS) exhibit reduced expression in skeletal muscle of diabetic and prediabetic humans. Moreover, these changes may be mediated by the transcriptional coactivator peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alph...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0401401101

    authors： Mootha VK,Handschin C,Arlow D,Xie X,St Pierre J,Sihag S,Yang W,Altshuler D,Puigserver P,Patterson N,Willy PJ,Schulman IG,Heyman RA,Lander ES,Spiegelman BM

    更新日期：2004-04-27 00:00:00

  • Lysine methyltransferase G9a is required for de novo DNA methylation and the establishment, but not the maintenance, of proviral silencing.

    abstract：:Methylation on lysine 9 of histone H3 (H3K9me) and DNA methylation play important roles in the transcriptional silencing of specific genes and repetitive elements. Both marks are detected on class I and II endogenous retroviruses (ERVs) in murine embryonic stem cells (mESCs). Recently, we reported that the H3K9-specif...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1014660108

    authors： Leung DC,Dong KB,Maksakova IA,Goyal P,Appanah R,Lee S,Tachibana M,Shinkai Y,Lehnertz B,Mager DL,Rossi F,Lorincz MC

    更新日期：2011-04-05 00:00:00

  • Myoglobin-mediated oxygen delivery to mitochondria of isolated cardiac myocytes.

    abstract：:Myoglobin-mediated oxygen delivery to intracellular mitochondria is demonstrated in cardiac myocytes isolated from the hearts of mature rats. Myocytes are held at high ambient oxygen pressure, 40-340 torr (5-45 kPa); sarcoplasmic myoglobin is fully oxygenated. In this condition oxygen availability does not limit respi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.21.7503

    authors： Wittenberg BA,Wittenberg JB

    更新日期：1987-11-01 00:00:00

  • Glutamate transporter GLAST controls synaptic wrapping by Bergmann glia and ensures proper wiring of Purkinje cells.

    abstract：:Astrocytes regulate synaptic transmission through controlling neurotransmitter concentrations around synapses. Little is known, however, about their roles in neural circuit development. Here we report that Bergmann glia (BG), specialized cerebellar astrocytes that thoroughly enwrap Purkinje cells (PCs), are essential ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1617330114

    authors： Miyazaki T,Yamasaki M,Hashimoto K,Kohda K,Yuzaki M,Shimamoto K,Tanaka K,Kano M,Watanabe M

    更新日期：2017-07-11 00:00:00

  • Spectrum of hot methane in astronomical objects using a comprehensive computed line list.

    abstract：:Hot methane spectra are important in environments ranging from flames to the atmospheres of cool stars and exoplanets. A new spectroscopic line list, 10to10, for (12)CH4 containing almost 10 billion transitions is presented. This comprehensive line list covers a broad spectroscopic range and is applicable for temperat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1324219111

    authors： Yurchenko SN,Tennyson J,Bailey J,Hollis MD,Tinetti G

    更新日期：2014-07-01 00:00:00

  • Multiple thyroid hormone-induced muscle growth and death programs during metamorphosis in Xenopus laevis.

    abstract：:Xenopus laevis tadpole tails contain fast muscle fibers oriented in chevrons and two pairs of slow muscle "cords" along the length of the tail. When tail resorption is inhibited by a number of different treatments, fast muscle but not the slow cord muscle still is lost, demonstrating that the fast tail muscle is a dir...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.182430599

    authors： Das B,Schreiber AM,Huang H,Brown DD

    更新日期：2002-09-17 00:00:00

  • CDK8/19 Mediator kinases potentiate induction of transcription by NFκB.

    abstract：:The nuclear factor-κB (NFκB) family of transcription factors has been implicated in inflammatory disorders, viral infections, and cancer. Most of the drugs that inhibit NFκB show significant side effects, possibly due to sustained NFκB suppression. Drugs affecting induced, but not basal, NFκB activity may have the pot...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1710467114

    authors： Chen M,Liang J,Ji H,Yang Z,Altilia S,Hu B,Schronce A,McDermott MSJ,Schools GP,Lim CU,Oliver D,Shtutman MS,Lu T,Stark GR,Porter DC,Broude EV,Roninson IB

    更新日期：2017-09-19 00:00:00