RhoGDIgamma: a GDP-dissociation inhibitor for Rho proteins with preferential expression in brain and pancreas.

abstract：:Stromal cell lines were established from canine long-term marrow cultures, cloned by limiting dilution, and maintained in stromal cell-conditioned medium. These cells grew adherent, maintained stable growth rate and morphology under standard conditions (in 20-30% conditioned medium; confluency, 70-90%), and supported ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huss R,Hong DS,McSweeney PA,Hoy CA,Deeg HJ

更新日期：1995-01-31 00:00:00

abstract：:Plant steroid hormones, brassinosteroids (BRs), are of great importance for plant growth and development. BRs signal through a cell surface receptor kinase, BRI1, and a GSK3-like kinase, BIN2, to regulate the BES1/BZR1 family of transcription factors, which directly bind to target gene promoters to activate or repress...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu X,Li L,Li L,Guo M,Chory J,Yin Y

更新日期：2008-05-27 00:00:00

abstract：:Nitric oxide (NO) has been implicated as a pathogenic mediator in a variety of central nervous system (CNS) disease states, including the animal model of multiple sclerosis (MS) and experimental allergic encephalomyelitis. We have examined post-mortem brain tissues collected from patients previously diagnosed with MS,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bagasra O,Michaels FH,Zheng YM,Bobroski LE,Spitsin SV,Fu ZF,Tawadros R,Koprowski H

更新日期：1995-12-19 00:00:00

abstract：:Reinstatement of neural activity is hypothesized to underlie our ability to mentally travel back in time to recover the context of a previous experience. We used intracranial recordings to directly examine the precise spatiotemporal extent of neural reinstatement as 32 participants with electrodes placed for seizure m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yaffe RB,Kerr MS,Damera S,Sarma SV,Inati SK,Zaghloul KA

更新日期：2014-12-30 00:00:00

abstract：:Members of the POU-homeodomain gene family encode transcriptional regulatory molecules that play important roles in terminal differentiation of many organ systems. Sperm-1 (Sprm-1) is a POU domain factor that is exclusively expressed in the differentiating male germ cell. We show here that the Sprm-1 protein is expres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pearse RV 2nd,Drolet DW,Kalla KA,Hooshmand F,Bermingham JR Jr,Rosenfeld MG

更新日期：1997-07-08 00:00:00

abstract：:The cell surface of embryonic peripheral neurons provides a mitogenic stimulus for Schwann cells. We report (i) the solubilization of this mitogenic activity from rat dorsal root ganglion neurons grown in tissue culture and (ii) the solubilization and partial purification of mitogenic activity from neonatal rat brains...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ratner N,Hong DM,Lieberman MA,Bunge RP,Glaser L

更新日期：1988-09-01 00:00:00

abstract：:NRSF/REST is a protein that silences transcription of a number of genes that contain a DNA element called the neuron-restrictive silencer element (NRSE). During embryogenesis, REST is expressed ubiquitously in nonneural cells, but is down-regulated during differentiation of neural progenitors into neurons. REST is als...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koenigsberger C,Chicca JJ 2nd,Amoureux MC,Edelman GM,Jones FS

更新日期：2000-02-29 00:00:00

abstract：:During development, organisms acquire three-dimensional (3D) shapes with important physiological consequences. While basic mechanisms underlying morphogenesis are known in eukaryotes, it is often difficult to manipulate them in vivo. To circumvent this issue, here we present a study of developing Vibrio cholerae biofi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fei C,Mao S,Yan J,Alert R,Stone HA,Bassler BL,Wingreen NS,Košmrlj A

更新日期：2020-04-07 00:00:00

abstract：:The swelling of secretory vesicles has been implicated in exocytosis, but the underlying mechanism of vesicle swelling remains largely unknown. Zymogen granules (ZGs), the membrane-bound secretory vesicles in exocrine pancreas, swell in response to GTP mediated by a G(alpha)i3 protein. Evidence is presented here that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cho SJ,Sattar AK,Jeong EH,Satchi M,Cho JA,Dash S,Mayes MS,Stromer MH,Jena BP

更新日期：2002-04-02 00:00:00

abstract：:Development of in vitro resistance to HN2 (also called mustargen or mechlorethamine hydrochloride), N,N'-bis(2-chloroethyl)-N-nitrosourea (BCNU), and cisplatin [cis-diamminedichloroplatinum(II)] was achieved in two human cell lines, the Raji/Burkitt lymphoma and a squamous cell carcinoma of the tongue. A 10- to 20-fol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frei E 3rd,Cucchi CA,Rosowsky A,Tantravahi R,Bernal S,Ervin TJ,Ruprecht RM,Haseltine WA

更新日期：1985-04-01 00:00:00

abstract：:The protein encoded by the neu protooncogene (human gene symbol NGL for neuro/glioblastoma-derived) is a member of the surface receptor/tyrosine kinase family. Though its structure suggests that it can transduce a transmembrane signal, neither its extracellular ligand nor its critical intracellular substrates are know...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flanagan JG,Leder P

更新日期：1988-11-01 00:00:00

abstract：:During CsCl isopycnic centrifugation at 20 mM Mg(++), Escherichia coli 30S ribosomal subunits specifically lose proteins S1, S2, S3, S5, S9, S10, and S14. The resultant 30S core is unable to stimulate the GTPase activity of EF-G in the presence of 50S subunits. Activity could be restored to a small extent by adding ba...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marsh RC,Parmeggiani A

更新日期：1973-01-01 00:00:00

abstract：:The expansion of machine learning to high-stakes application domains such as medicine, finance, and criminal justice, where making informed decisions requires clear understanding of the model, has increased the interest in interpretable machine learning. The widely used Classification and Regression Trees (CART) have ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luna JM,Gennatas ED,Ungar LH,Eaton E,Diffenderfer ES,Jensen ST,Simone CB 2nd,Friedman JH,Solberg TD,Valdes G

更新日期：2019-10-01 00:00:00

abstract：:Integrated hepatitis B virus (HBV) DNA is almost invariably found in hepatocellular carcinomas (HCC) which develop in HBV carriers. Integrated HBV DNAs from two single-integration HCCs (C3 and C4) have been cloned, and the cellular integration sites have been analyzed. Integrated HBV DNA of C3 is present in chromosome...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hino O,Shows TB,Rogler CE

更新日期：1986-11-01 00:00:00

abstract：:Tumor necrosis factor (TNF) is a macrophage product under active study as an anticancer drug. However, this agent can be very toxic and has been implicated in the pathogenesis of endotoxic shock. After intravenous injection of human recombinant TNF (4 micrograms/g), growing rats showed an unusual constellation of phys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kettelhut IC,Fiers W,Goldberg AL

更新日期：1987-06-01 00:00:00

abstract：:Alpha-actinin-4 is a widely expressed protein that employs an actin-binding site with two calponin homology domains to crosslink actin filaments (F-actin) in a Ca(2+)-sensitive manner in vitro. An inherited, late-onset form of kidney failure is caused by point mutations in the alpha-actinin-4 actin-binding domain. Her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weins A,Schlondorff JS,Nakamura F,Denker BM,Hartwig JH,Stossel TP,Pollak MR

更新日期：2007-10-09 00:00:00

abstract：:Acute and chronic injuries to the heart result in perturbation of intracellular calcium signaling, which leads to pathological cardiac hypertrophy and remodeling. Calcium/calmodulin-dependent protein kinase II (CaMKII) has been implicated in the transduction of calcium signals in the heart, but the specific isoforms o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Backs J,Backs T,Neef S,Kreusser MM,Lehmann LH,Patrick DM,Grueter CE,Qi X,Richardson JA,Hill JA,Katus HA,Bassel-Duby R,Maier LS,Olson EN

更新日期：2009-02-17 00:00:00

abstract：:We have studied ten phenotypically similar patients with complete androgen insensitivity. All of the patients tested had significantly elevated serum luteinizing hormone and plasma androgens within or above the normal adult male range. On the basis of specific dihydrotestosterone binding by skin fibroblasts, we identi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amrhein JA,Meyer WJ 3rd,Jones HW Jr,Migeon CJ

更新日期：1976-03-01 00:00:00

abstract：:We have made direct, quantitative measurements of the lateral motion and age-dependent distribution of acetylcholine receptors (AChR) on the surface of rat myotubes in primary culture. AChR were fluorescently marked with tetramethylrhodamine-labeled alpha-bungarotoxin and AChR lateral motion was measured by the fluore...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Axelrod D,Ravdin P,Koppel DE,Schlessinger J,Webb WW,Elson EL,Podleski TR

更新日期：1976-12-01 00:00:00

abstract：:The rate of change of climate codetermines the global warming impacts on natural and socioeconomic systems and their capabilities to adapt. Establishing past rates of climate change from temperature proxy data remains difficult given their limited spatiotemporal resolution. In contrast, past greenhouse gas radiative f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Joos F,Spahni R

更新日期：2008-02-05 00:00:00

abstract：:Monospecific antibodies were produced in vitro by fusing mouse myeloma cells with spleen cells from a BALB/c mouse immunized with rat skeletal myofibrils. After cloning 3 times on agarose, two stable clones were obtained and chosen for further characterization. The first clone, JLB1, produced an antibody that recogniz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin JJ

更新日期：1981-04-01 00:00:00

abstract：:Transposition is a multistep process in which a transposable element DNA sequence moves from its original genetic location to a new site. Early steps in this process include the formation of a transposition complex in which the end sequences of the transposable element are brought together in a structurally precise fa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goryshin IYu,Kil YV,Reznikoff WS

更新日期：1994-11-08 00:00:00

abstract：:A descriptive medium for the presentation of protein structure has been developed and used to evaluate the structure of the active site of bovine trypsin (EC 3.4.21.4). This technique, involving advanced computer graphics technology, permits the facile display of a representation of the molecular surface of proteins o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feldmann RJ,Bing DH,Furie BC,Furie B

更新日期：1978-11-01 00:00:00

abstract：:In oxygenic photosynthetic eukaryotes, the hydroxylated carotenoid zeaxanthin is produced from preexisting violaxanthin upon exposure to excess light conditions. Zeaxanthin binding to components of the photosystem II (PSII) antenna system has been investigated thoroughly and shown to help in the dissipation of excess ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ballottari M,Alcocer MJ,D'Andrea C,Viola D,Ahn TK,Petrozza A,Polli D,Fleming GR,Cerullo G,Bassi R

更新日期：2014-06-10 00:00:00

abstract：:Transfer of organelle DNA into the nuclear genome has been significant in eukaryotic evolution, because it appears to be the origin of many nuclear genes. Most studies on organelle DNA transfer have been restricted to evolutionary events but experimental systems recently became available to monitor the process in real...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang CY,Ayliffe MA,Timmis JN

更新日期：2004-06-29 00:00:00

abstract：:The inference of the evolutionary history of a set of languages is a complex problem. Although some languages are known to be related through descent from common ancestral languages, for other languages determining whether such a relationship holds is itself a difficult problem. In this paper we report on new methods,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warnow T

更新日期：1997-06-24 00:00:00

abstract：:Recent advances in metabolic engineering have demonstrated that microbial biosynthesis can provide a viable alternative to chemical synthesis for the production of bulk and fine chemicals. Introduction of a new biosynthetic pathway typically requires the expression of multiple heterologous enzymes in the production ho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Michener JK,Nielsen J,Smolke CD

更新日期：2012-11-20 00:00:00

abstract：:The usual way to compute Hartree-Fock type functions for molecules is by an expansion of the one-electron functions (molecular orbitals) in a linear combination of analytical functions (LCAO-MO-SCF, linear combination of atomic orbitals-Molecular Orbital-Self Consistent field). The expansion coefficients are obtained ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clementi E

更新日期：1972-10-01 00:00:00

abstract：:The fate of two recently described human B lymphocyte-specific antigens (B1 and B2) was studied after B-cell activation in vivo and in vitro. Whereas both B1 and B2 were present on virtually all B cells from normal lymph nodes, B2 was absent from approximately 50% of B cells from hyperplastic lymph nodes. When B cells...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stashenko P,Nadler LM,Hardy R,Schlossman SF

更新日期：1981-06-01 00:00:00

abstract：:A23187 is a carboxylic antibiotic that selectively transfers calcium, magnesium, and other divalent cations across biologic membranes. This ionophore was found to produce morphologic blast transformation, DNA synthesis, and mitosis in human lymphocytes. Several hours of ionophore-cell contact were necessary to produce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luckasen JR,White JG,Kersey JH

更新日期：1974-12-01 00:00:00