Abstract:
:We have made direct, quantitative measurements of the lateral motion and age-dependent distribution of acetylcholine receptors (AChR) on the surface of rat myotubes in primary culture. AChR were fluorescently marked with tetramethylrhodamine-labeled alpha-bungarotoxin and AChR lateral motion was measured by the fluoresence photobleaching recovery technique. We found two coexisting distinct classes of AChR: (i) mobile, uniformly distributed AChR that appear on all myotubes shortly after fusion from myoblasts; and (ii) immobile, dense, highly granular AChR in patches of 10-60 mum size that appear shortly after fusion and disappear after myotubes have become extensively interconnected. In addition, evidence of turnover of AChR labeled with tetramethylrhodamine-alpha-bungarotoxin is seen in the gradual internalization of surface fluorescence within 36 hr after labeling. The relevance of these results to an understanding of the membrane dynamics and localization of muscle AChR is discussed.
journal_name
Proc Natl Acad Sci U S Aauthors
Axelrod D,Ravdin P,Koppel DE,Schlessinger J,Webb WW,Elson EL,Podleski TRdoi
10.1073/pnas.73.12.4594keywords:
subject
Has Abstractpub_date
1976-12-01 00:00:00pages
4594-8issue
12eissn
0027-8424issn
1091-6490journal_volume
73pub_type
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