Abstract:
:The swelling of secretory vesicles has been implicated in exocytosis, but the underlying mechanism of vesicle swelling remains largely unknown. Zymogen granules (ZGs), the membrane-bound secretory vesicles in exocrine pancreas, swell in response to GTP mediated by a G(alpha)i3 protein. Evidence is presented here that the water channel aquaporin-1 (AQP1) is present in the ZG membrane and participates in rapid GTP-induced vesicular water gating and swelling. Isolated ZGs exhibit low basal water permeability. However, exposure of granules to GTP results in a marked potentiation of water entry. Treatment of ZGs with the known water channel inhibitor Hg2+ is accompanied by a reversible loss in both the basal and GTP-stimulatable water entry and vesicle swelling. Introduction of AQP1-specific antibody raised against the carboxyl-terminal domain of AQP1 blocks GTP-stimulable swelling of vesicles. Our results demonstrate that AQP1 associated at the ZG membrane is involved in basal as well as GTP-induced rapid gating of water in ZGs of the exocrine pancreas.
journal_name
Proc Natl Acad Sci U S Aauthors
Cho SJ,Sattar AK,Jeong EH,Satchi M,Cho JA,Dash S,Mayes MS,Stromer MH,Jena BPdoi
10.1073/pnas.072083499keywords:
subject
Has Abstractpub_date
2002-04-02 00:00:00pages
4720-4issue
7eissn
0027-8424issn
1091-6490pii
072083499journal_volume
99pub_type
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