当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > neu protooncogene fused to an immunoglobulin heavy chain gene requires immunoglobulin light chain for cell surface expression and oncogenic transformation.

neu protooncogene fused to an immunoglobulin heavy chain gene requires immunoglobulin light chain for cell surface expression and oncogenic transformation.

Abstract：

:The protein encoded by the neu protooncogene (human gene symbol NGL for neuro/glioblastoma-derived) is a member of the surface receptor/tyrosine kinase family. Though its structure suggests that it can transduce a transmembrane signal, neither its extracellular ligand nor its critical intracellular substrates are known. To explore the functional properties of the protein encoded by neu, we created a fusion gene that joins the cytoplasmic domain of neu to the extracellular portion of an immunoglobulin heavy chain. The localization of the fusion polypeptide can then be controlled by coexpression with immunoglobulin light chain. In the absence of light chain, the heavy chain-neu polypeptide is expressed intracellularly and has no transforming activity. By contrast, in the presence of light chain the fusion polypeptide is expressed at the cell surface and produces tumorigenic foci. Thus, transformation apparently requires expression at the cell surface, where the neu intracellular domain can interact with components that are localized to the plasma membrane. The fusion protein is active in cellular transformation when the transmembrane domain is derived either from neu or from immunoglobulin, indicating that the neu transmembrane domain is not specifically required for transformation, although neu activation in tumors is known to result from a point mutation in this region. The extracellular immunoglobulin heavy and light chain domains of the fusion protein form a functional binding site that allows antigen to modulate its activity, reversing the transforming effect.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Flanagan JG,Leder P

doi

10.1073/pnas.85.21.8057

subject

Has Abstract

pub_date

1988-11-01 00:00:00

pages

8057-61

issue

21

eissn

0027-8424

issn

1091-6490

journal_volume

85

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Neuropsin (OPN5)-mediated photoentrainment of local circadian oscillators in mammalian retina and cornea.

    abstract：:The molecular circadian clocks in the mammalian retina are locally synchronized by environmental light cycles independent of the suprachiasmatic nuclei (SCN) in the brain. Unexpectedly, this entrainment does not require rods, cones, or melanopsin (OPN4), possibly suggesting the involvement of another retinal photopigm...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1516259112

    authors： Buhr ED,Yue WW,Ren X,Jiang Z,Liao HW,Mei X,Vemaraju S,Nguyen MT,Reed RR,Lang RA,Yau KW,Van Gelder RN

    更新日期：2015-10-20 00:00:00

  • Isolation of actin-containing transmembrane complexes from ascites adenocarcinoma sublines having mobile and immobile receptors.

    abstract：:The molecular nature of the cell surface-cytoskeleton interaction in microvilli isolated from ascites 13762 rat mammary adenocarcinoma sublines with immobile (MAT-C1) and mobile (MAT-B1) receptors was investigated by extraction and fractionation studies on the microvillar membranes. Extraction of membranes from MAT-C1...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.2.430

    authors： Carraway CA,Jung G,Carraway KL

    更新日期：1983-01-01 00:00:00

  • Fungal prion HET-s as a model for structural complexity and self-propagation in prions.

    abstract：:The highly ordered and reproducible structure of the fungal prion HET-s makes it an excellent model system for studying the inherent properties of prions, self-propagating infectious proteins that have been implicated in a number of fatal diseases. In particular, the HET-s prion-forming domain readily folds into a rel...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1322933111

    authors： Wan W,Stubbs G

    更新日期：2014-04-08 00:00:00

  • Raman resonance of electron donor/acceptor complex.

    abstract：:The resonance Raman excitation profiles of a number of charge transfer transitions in electron donor/acceptor complexes with tetracyanoethylene as acceptor in solution at room temperature are reported and compared with absorption and fluorescence spectra of these complexes. All complexes show distinct anomalies which ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.11.4196

    authors： Michaelian KH,Rieckhoff KE,Voigt EM

    更新日期：1975-11-01 00:00:00

  • The choroid plexus epithelium is the site of the organic anion transport protein in the brain.

    abstract：:The mRNA for organic anion transport protein (oatp) was previously shown to be present in abundance in liver and kidney, and in small amounts in brain. Data obtained from experiments with reverse transcriptase-PCR techniques and in situ hybridization analysis showed that the oatp mRNA is present within the brain, loca...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.1.283

    authors： Angeletti RH,Novikoff PM,Juvvadi SR,Fritschy JM,Meier PJ,Wolkoff AW

    更新日期：1997-01-07 00:00:00

  • Identification of genes and gene products necessary for bacterial bioluminescence.

    abstract：:Expression of luminescence in Escherichia coli was recently achieved by cloning genes from the marine bacterium Vibrio fischeri. One DNA fragment on a hybrid plasmid encoded regulatory functions and enzymatic activities necessary for light production. We report the results of a genetic analysis to identify the lumines...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.13.4154

    authors： Engebrecht J,Silverman M

    更新日期：1984-07-01 00:00:00

  • Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells.

    abstract：:This report describes an unbiased method for systematically determining gene function in mammalian cells. A total of 20,704 predicted human full-length cDNAs were tested for induction of the IL-8 promoter. A number of genes, including those for cytokines, receptors, adapters, kinases, and transcription factors, were i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1932773100

    authors： Iourgenko V,Zhang W,Mickanin C,Daly I,Jiang C,Hexham JM,Orth AP,Miraglia L,Meltzer J,Garza D,Chirn GW,McWhinnie E,Cohen D,Skelton J,Terry R,Yu Y,Bodian D,Buxton FP,Zhu J,Song C,Labow MA

    更新日期：2003-10-14 00:00:00

  • Bipolar switching by HCN voltage sensor underlies hyperpolarization activation.

    abstract：:Despite sharing a common architecture with archetypal voltage-gated ion channels (VGICs), hyperpolarization- and cAMP-activated ion (HCN) channels open upon hyperpolarization rather than depolarization. The basic motions of the voltage sensor and pore gates are conserved, implying that these domains are inversely coup...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1816724116

    authors： Cowgill J,Klenchin VA,Alvarez-Baron C,Tewari D,Blair A,Chanda B

    更新日期：2019-01-08 00:00:00

  • Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities.

    abstract：:Aliphatic hydrocarbons such as fatty alcohols and petroleum-derived alkanes have numerous applications in the chemical industry. In recent years, the renewable synthesis of aliphatic hydrocarbons has been made possible by engineering microbes to overaccumulate fatty acids. However, to generate end products with the de...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1216516110

    authors： Akhtar MK,Turner NJ,Jones PR

    更新日期：2013-01-02 00:00:00

  • Klotho is associated with VEGF receptor-2 and the transient receptor potential canonical-1 Ca2+ channel to maintain endothelial integrity.

    abstract：:Klotho is a circulating protein, and Klotho deficiency disturbs endothelial integrity, but the molecular mechanism is not fully clarified. We report that vascular endothelium in Klotho-deficient mice showed hyperpermeability with increased apoptosis and down-regulation of vascular endothelial (VE)-cadherin because of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1008544107

    authors： Kusaba T,Okigaki M,Matui A,Murakami M,Ishikawa K,Kimura T,Sonomura K,Adachi Y,Shibuya M,Shirayama T,Tanda S,Hatta T,Sasaki S,Mori Y,Matsubara H

    更新日期：2010-11-09 00:00:00

  • Control of a Salmonella virulence operon by proline-charged tRNA(Pro).

    abstract：:The intracellular pathogen Salmonella enterica serovar Typhimurium requires the mgtC gene to cause disease. The mgtC transcript includes a long leader region that harbors a short proline codon-rich ORF--termed mgtP--the translation of which is predicted to favor formation of one of two alternative stem-loop structures...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1316209111

    authors： Lee EJ,Choi J,Groisman EA

    更新日期：2014-02-25 00:00:00

  • Nonlinear trends in abundance and diversity and complex responses to climate change in Arctic arthropods.

    abstract：:Time series data on arthropod populations are critical for understanding the magnitude, direction, and drivers of change. However, most arthropod monitoring programs are short-lived and restricted in taxonomic resolution. Monitoring data from the Arctic are especially underrepresented, yet critical to uncovering and u...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2002557117

    authors： Høye TT,Loboda S,Koltz AM,Gillespie MAK,Bowden JJ,Schmidt NM

    更新日期：2021-01-12 00:00:00

  • Genetic mapping of the inversion loop in bacteriophage Mu DNA.

    abstract：:An inversion loop seen in heteroduplex mapping of the DNA of mature Mu phage induced from a lysogen is observed also in defective lambda phage carrying one end of Mu. 14% of the DNA of Mu, including this region, designated the G loop, is shown to be to the right of all known genes in the prophage map. The inhomogeneou...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.70.7.2153

    authors： Daniell E,Boram W,Abelson J

    更新日期：1973-07-01 00:00:00

  • Mutational switch of an IL-6 response to an interferon-gamma-like response.

    abstract：:Signaling through Janus kinases (JAKs) and signal transducers and activators of transcription (STATs) is central to the responses to the majority of cytokines and some growth factors, including the interferons (IFNs) and the IL-6 family of cytokines. The biological responses to stimulation through the widely distribut...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.122236099

    authors： Costa-Pereira AP,Tininini S,Strobl B,Alonzi T,Schlaak JF,Is'harc H,Gesualdo I,Newman SJ,Kerr IM,Poli V

    更新日期：2002-06-11 00:00:00

  • Gene therapy of murine teratocarcinoma: separate functions for insulin-like growth factors I and II in immunogenicity and differentiation.

    abstract：:Teratocarcinoma is a germ-line carcinoma giving rise to an embryoid tumor with structures derived from the three embryonic layers: mesoderm, endoderm, and ectoderm. Teratocarcinoma is widely used as an in vitro model system to study regulation of cell determination and differentiation during mammalian embryogenesis. M...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.13.6088

    authors： Trojan J,Johnson TR,Rudin SD,Blossey BK,Kelley KM,Shevelev A,Abdul-Karim FW,Anthony DD,Tykocinski ML,Ilan J

    更新日期：1994-06-21 00:00:00

  • The Usher syndrome proteins cadherin 23 and harmonin form a complex by means of PDZ-domain interactions.

    abstract：:Usher syndrome type 1 (USH1) patients suffer from sensorineuronal deafness, vestibular dysfunction, and visual impairment. Several genetic loci have been linked to USH1, and four of the relevant genes have been identified. They encode the unconventional myosin VIIa, the PDZ-domain protein harmonin, and the putative ad...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.232579599

    authors： Siemens J,Kazmierczak P,Reynolds A,Sticker M,Littlewood-Evans A,Müller U

    更新日期：2002-11-12 00:00:00

  • A conserved molecular basis for photoperiod adaptation in two temperate legumes.

    abstract：:Legumes were among the first plant species to be domesticated, and accompanied cereals in expansion of agriculture from the Fertile Crescent into diverse environments across the Mediterranean basin, Europe, Central Asia, and the Indian subcontinent. Although several recent studies have outlined the molecular basis for...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1207943110

    authors： Weller JL,Liew LC,Hecht VF,Rajandran V,Laurie RE,Ridge S,Wenden B,Vander Schoor JK,Jaminon O,Blassiau C,Dalmais M,Rameau C,Bendahmane A,Macknight RC,Lejeune-Hénaut I

    更新日期：2012-12-18 00:00:00

  • Nondiffusive mechanisms enhance protein uptake rates in ion exchange particles.

    abstract：:Scanning confocal fluorescence microscopy and multiphoton fluorescence microscopy were used to image the uptake of the protein lysozyme into individual ion exchange chromatography particles in a packed bed in real time. Self-sharpening concentration fronts penetrating into the particles were observed at low salt conce...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0237084100

    authors： Dziennik SR,Belcher EB,Barker GA,DeBergalis MJ,Fernandez SE,Lenhoff AM

    更新日期：2003-01-21 00:00:00

  • Alkane biosynthesis by decarbonylation of aldehydes catalyzed by a particulate preparation from Pisum sativum.

    abstract：:Mechanism of enzymatic conversion of a fatty acid to the corresponding alkane by the loss of the carboxyl carbon was investigated with particulate preparations from Pisum sativum. A heavy particulate preparation (sp. gr., 1.30 g/cm3) isolated by two density-gradient centrifugation steps catalyzed conversion of octadec...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.21.6613

    authors： Cheesbrough TM,Kolattukudy PE

    更新日期：1984-11-01 00:00:00

  • ABCC6 prevents ectopic mineralization seen in pseudoxanthoma elasticum by inducing cellular nucleotide release.

    abstract：:Pseudoxanthoma elasticum (PXE) is an autosomal recessive disease characterized by progressive ectopic mineralization of the skin, eyes, and arteries, for which no effective treatment exists. PXE is caused by inactivating mutations in the gene encoding ATP-binding cassette sub-family C member 6 (ABCC6), an ATP-dependen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1319582110

    authors： Jansen RS,Küçükosmanoglu A,de Haas M,Sapthu S,Otero JA,Hegman IE,Bergen AA,Gorgels TG,Borst P,van de Wetering K

    更新日期：2013-12-10 00:00:00

  • Simian virus 40 minichromosomes as targets for retroviral integration in vivo.

    abstract：:We present a method for studying multiple retroviral integration events into a small DNA target in vivo. Episomal simian virus 40 (SV40) genomes established by infection of CV-1 cells served as integration targets during subsequent infection with murine leukemia virus (MLV). Using a PCR-based assay for the abundance a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.19.9237

    authors： Pryciak PM,Müller HP,Varmus HE

    更新日期：1992-10-01 00:00:00

  • Elastocapillary interactions on nematic films.

    abstract：:Rod-like colloids distort fluid interfaces and interact by capillarity. We explore this interaction at the free surface of aligned nematic liquid crystal films. Naive comparison of capillary and elastic energies suggests that particle assembly would be determined solely by surface tension. Here, we demonstrate that, u...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1504817112

    authors： Liu IB,Gharbi MA,Ngo VL,Kamien RD,Yang S,Stebe KJ

    更新日期：2015-05-19 00:00:00

  • Genes encoding major light-harvesting polypeptides are clustered on the genome of the cyanobacterium Fremyella diplosiphon.

    abstract：:The polypeptide composition of the phycobilisome, the major light-harvesting complex of prokaryotic cyanobacteria and certain eukaryotic algae, can be modulated by different light qualities in cyanobacteria exhibiting chromatic adaptation. We have identified genomic fragments encoding a cluster of phycobilisome polype...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.11.3924

    authors： Conley PB,Lemaux PG,Lomax TL,Grossman AR

    更新日期：1986-06-01 00:00:00

  • Intersection of FOXO- and RUNX1-mediated gene expression programs in single breast epithelial cells during morphogenesis and tumor progression.

    abstract：:Gene expression networks are complicated by the assortment of regulatory factors that bind DNA and modulate transcription combinatorially. Single-cell measurements can reveal biological mechanisms hidden by population averages, but their value has not been fully explored in the context of mRNA regulation. Here, we ada...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1103423108

    authors： Wang L,Brugge JS,Janes KA

    更新日期：2011-10-04 00:00:00

  • Cell-free synthesis of a specific lipoprotein of the Escherichia coli outer membrane directed by purified messenger RNA.

    abstract：:A specific lipoprotein of the E. coli outer membrane has been synthesized in a cell-free system directed by purified messenger RNA. The mRNA for the lipoprotein was purified as 7S RNA about 250-fold from exponentially growing cells. Protein synthesis of the cell-free system was totally dependent upon the addition of t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.10.4149

    authors： Hirashima A,Wang S,Inouye M

    更新日期：1974-10-01 00:00:00

  • Quantification of protein half-lives in the budding yeast proteome.

    abstract：:A complete description of protein metabolism requires knowledge of the rates of protein production and destruction within cells. Using an epitope-tagged strain collection, we measured the half-life of >3,750 proteins in the yeast proteome after inhibition of translation. By integrating our data with previous measureme...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0605420103

    authors： Belle A,Tanay A,Bitincka L,Shamir R,O'Shea EK

    更新日期：2006-08-29 00:00:00

  • A common variant in the 3'UTR of the GRIK4 glutamate receptor gene affects transcript abundance and protects against bipolar disorder.

    abstract：:Underactivity of the glutamatergic system is an attractive model for the pathophysiology of several major mental illnesses. We previously described a chromosome abnormality disrupting the kainate class ionotropic glutamate receptor gene, GRIK4/KA1, in an individual with schizophrenia and learning disability (mental re...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0800643105

    authors： Pickard BS,Knight HM,Hamilton RS,Soares DC,Walker R,Boyd JK,Machell J,Maclean A,McGhee KA,Condie A,Porteous DJ,St Clair D,Davis I,Blackwood DH,Muir WJ

    更新日期：2008-09-30 00:00:00

  • Acetylcholine receptor-alpha-bungarotoxin interactions: determination of the region-to-region contacts by peptide-peptide interactions and molecular modeling of the receptor cavity.

    abstract：:In previous studies from this laboratory, the binding regions of alpha-neurotoxins on human and Torpedo acetylcholine (AcCho) receptors (AcChoRs) and the binding regions for the receptor on the toxin were characterized with synthetic peptides of the respective molecules. In the present work, peptides representing the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.16.6156

    authors： Ruan KH,Spurlino J,Quiocho FA,Atassi MZ

    更新日期：1990-08-01 00:00:00

  • E2F1 and E1A(12S) have a homologous activation domain regulated by RB and CBP.

    abstract：:The E2F1 transcription factor has a well-characterized activation domain at its C terminus and the E1A protein has a recently defined activation domain at its N terminus. Here we show that these activation domains are highly related in sequence. The sequence homology reflects, at least partly, the conservation of comm...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.4.1439

    authors： Trouche D,Kouzarides T

    更新日期：1996-02-20 00:00:00

  • Complete amino acid sequence of human hemopexin, the heme-binding protein of serum.

    abstract：:We have determined the complete primary structure of human hemopexin, a plasma beta-glycoprotein that specifically binds one heme with high affinity and transports it to hepatocytes for salvage of the iron. Human hemopexin (Mr approximately equal to 63,000) consists of a single polypeptide chain containing 439 amino a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.1.73

    authors： Takahashi N,Takahashi Y,Putnam FW

    更新日期：1985-01-01 00:00:00