Cell-free synthesis of a specific lipoprotein of the Escherichia coli outer membrane directed by purified messenger RNA.

Abstract:

:A specific lipoprotein of the E. coli outer membrane has been synthesized in a cell-free system directed by purified messenger RNA. The mRNA for the lipoprotein was purified as 7S RNA about 250-fold from exponentially growing cells. Protein synthesis of the cell-free system was totally dependent upon the addition of the purified mRNA. The product of the cell-free system was identified as the specific lipoprotein by immunoprecipitation and by peptide mapping.

authors

Hirashima A,Wang S,Inouye M

doi

10.1073/pnas.71.10.4149

subject

Has Abstract

pub_date

1974-10-01 00:00:00

pages

4149-53

issue

10

eissn

0027-8424

issn

1091-6490

journal_volume

71

pub_type

杂志文章
  • Phospholipase D-catalyzed hydrolysis of phosphatidylcholine provides the choline precursor for acetylcholine synthesis in a human neuronal cell line.

    abstract::To identify the metabolic pathway that generates choline (Cho) for acetylcholine (AcCho) from its storage pool in membrane phosphatidylcholine (PtdCho), human neuronal cells (LA-N-2) were radioisotopically labeled with 1-O-hexadecyl-2-hydroxy-sn-glycero(3)phospho[14C]choline. The compound was efficiently taken up by t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.21.10086

    authors: Lee HC,Fellenz-Maloney MP,Liscovitch M,Blusztajn JK

    更新日期:1993-11-01 00:00:00

  • Shifting eating to the circadian rest phase misaligns the peripheral clocks with the master SCN clock and leads to a metabolic syndrome.

    abstract::The light-entrained master central circadian clock (CC) located in the suprachiasmatic nucleus (SCN) not only controls the diurnal alternance of the active phase (the light period of the human light-dark cycle, but the mouse dark period) and the rest phase (the human dark period, but the mouse light period), but also ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1519807112

    authors: Mukherji A,Kobiita A,Damara M,Misra N,Meziane H,Champy MF,Chambon P

    更新日期:2015-12-01 00:00:00

  • Junctophilin-4, a component of the endoplasmic reticulum-plasma membrane junctions, regulates Ca2+ dynamics in T cells.

    abstract::Orai1 and stromal interaction molecule 1 (STIM1) mediate store-operated Ca(2+) entry (SOCE) in immune cells. STIM1, an endoplasmic reticulum (ER) Ca(2+) sensor, detects store depletion and interacts with plasma membrane (PM)-resident Orai1 channels at the ER-PM junctions. However, the molecular composition of these ju...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1524229113

    authors: Woo JS,Srikanth S,Nishi M,Ping P,Takeshima H,Gwack Y

    更新日期:2016-03-08 00:00:00

  • Casimir light: a glimpse.

    abstract::Light emission produced by the reversible collapse of a cavity in a dielectric medium is given an initial, simplified treatment. The agreement between planar and spherical shapes indicates the volume nature of the effect. ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.3.958

    authors: Schwinger J

    更新日期:1993-02-01 00:00:00

  • Network topology for the formation of solvated electrons in binary CaO-Al2O3 composition glasses.

    abstract::Glass formation in the CaO-Al2O3 system represents an important phenomenon because it does not contain typical network-forming cations. We have produced structural models of CaO-Al2O3 glasses using combined density functional theory-reverse Monte Carlo simulations and obtained structures that reproduce experiments (X-...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1300908110

    authors: Akola J,Kohara S,Ohara K,Fujiwara A,Watanabe Y,Masuno A,Usuki T,Kubo T,Nakahira A,Nitta K,Uruga T,Weber JK,Benmore CJ

    更新日期:2013-06-18 00:00:00

  • Seminoma-derived Nagao isozyme is encoded by a germ-cell alkaline phosphatase gene.

    abstract::A full-length placental alkaline phosphatase (PLAP) cDNA was used to identify and clone the PLAP-like Nagao isozyme gene from human genomic libraries. The entire nucleotide sequence of the gene reveals the existence of 11 exons interrupted by 10 small introns (76-427 base pairs). Putative regulatory sequences have bee...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.9.3024

    authors: Millán JL,Manes T

    更新日期:1988-05-01 00:00:00

  • The Escherichia coli RuvB branch migration protein forms double hexameric rings around DNA.

    abstract::The RuvB protein is induced in Escherichia coli as part of the SOS response to DNA damage. It is required for genetic recombination and the postreplication repair of DNA. In vitro, the RuvB protein promotes the branch migration of Holliday junctions and has a DNA helicase activity in reactions that require ATP hydroly...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.16.7618

    authors: Stasiak A,Tsaneva IR,West SC,Benson CJ,Yu X,Egelman EH

    更新日期:1994-08-02 00:00:00

  • Hyper innate responses in neonates lead to increased morbidity and mortality after infection.

    abstract::Neonates suffer high morbidity and mortality in infection, presumably because of the lack of a fully developed adaptive and innate immune system. Evidence of poor innate responses in neonates has been shown by using a model that sensitizes the host to Toll-like receptor (TLR)-mediated inflammation with d-galactosamine...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0800152105

    authors: Zhao J,Kim KD,Yang X,Auh S,Fu YX,Tang H

    更新日期:2008-05-27 00:00:00

  • beta-endorphin is a potent analgesic agent.

    abstract::beta-Endorphin, an opiate-like peptide, has potent antinociceptive properties when it is administered directly into the brain and assayed in the the tail-flick, hot-plate, and writhing tests in mice and in the wet shake test in rats. On a molar basis, beta-endorphin is 18 to 33 times more potent than morphine and its ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.73.8.2895

    authors: Loh HH,Tseng LF,Wei E,Li CH

    更新日期:1976-08-01 00:00:00

  • Identification of Id4 as a regulator of BRCA1 expression by using a ribozyme-library-based inverse genomics approach.

    abstract::Expression of the breast and ovarian cancer susceptibility gene BRCA1 is down-regulated in sporadic breast and ovarian cancer cases. Therefore, the identification of genes involved in the regulation of BRCA1 expression might lead to new insights into the pathogenesis and treatment of these tumors. In the present study...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.98.1.130

    authors: Beger C,Pierce LN,Kruger M,Marcusson EG,Robbins JM,Welcsh P,Welch PJ,Welte K,King MC,Barber JR,Wong-Staal F

    更新日期:2001-01-02 00:00:00

  • Differentiation of myoblast cell lines and biological methylation: 3-deazaadenosine stimulates formation of multinucleated myofibers.

    abstract::Treatment of myoblast cell lines with 3-deazaadenosine stimulates differentiation into myofibers. Myoblast clone L5/ 3B5 , which does not form myofibers after 6 days in fusion medium, was stimulated to form myofibers after 5 days of culture in fusion medium containing 50 microM 3-deazaadenosine. Myoblast clone L5/ 3C4...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.81.10.3064

    authors: Scarpa S,Strom R,Bozzi A,Aksamit RR,Backlund PS Jr,Chen J,Cantoni GL

    更新日期:1984-05-01 00:00:00

  • Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro.

    abstract::The enhancer-binding factor NF-kappa B, which is found only in cells that transcribe immunoglobulin light chain genes, has been purified from nuclear extracts of Namalwa cells (human Burkitt lymphoma cells) by sequence-specific DNA affinity chromatography. The purified NF-kappa B has been identified as a 51-kDa polype...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.13.4700

    authors: Kawakami K,Scheidereit C,Roeder RG

    更新日期:1988-07-01 00:00:00

  • Fusion of dipalmitoylphosphatidylcholine vesicle membranes induced by concanavalin A.

    abstract::The temperature dependence of fatty acid spin label resonance spectra and freeze fracture micrographs of sonicated dipalmitoylphosphatidylcholine vesicles in the absence and presence of concanavalin A demonstrate a strong interaction of concanavalin A with these lipid membranes, which results in fusion of the vesicles...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.72.11.4409

    authors: van der Bosch J,McConnell M

    更新日期:1975-11-01 00:00:00

  • Hippocampus-dependent emergence of spatial sequence coding in retrosplenial cortex.

    abstract::Retrosplenial cortex (RSC) is involved in visuospatial integration and spatial learning, and RSC neurons exhibit discrete, place cell-like sequential activity that resembles the population code of space in hippocampus. To investigate the origins and population dynamics of this activity, we combined longitudinal cellul...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1803224115

    authors: Mao D,Neumann AR,Sun J,Bonin V,Mohajerani MH,McNaughton BL

    更新日期:2018-07-31 00:00:00

  • Studies of Lp-lipoprotein as a quantitative genetic trait.

    abstract::Sera from 11 individuals which were classified as Lp(a-) by direct gel diffusion and by absorption tests showed the presence of Lp(a) lipoprotein when the sera were concentrated 120-fold. This Lp(a) antigen was demonstrated by gel diffusion to be indistinguishable from Lp-lipoprotein isolated from known Lp(a+) sera. T...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.66.1.99

    authors: Harvie NR,Schultz JS

    更新日期:1970-05-01 00:00:00

  • Quinacrine promotes replication and conformational mutation of chronic wasting disease prions.

    abstract::Quinacrine's ability to reduce levels of pathogenic prion protein (PrP(Sc)) in mouse cells infected with experimentally adapted prions led to several unsuccessful clinical studies in patients with prion diseases, a 10-y investment to understand its mechanism of action, and the production of related compounds with expe...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1322377111

    authors: Bian J,Kang HE,Telling GC

    更新日期:2014-04-22 00:00:00

  • Myofibrillogenesis visualized in living embryonic cardiomyocytes.

    abstract::Myofibril formation was visualized in cultured live cardiomyocytes that were transfected with plasmids expressing green fluorescent protein (GFP) linked to the Z-band protein, alpha-actinin. The expression of this fluorescent protein provided an in vivo label for structures containing alpha-actinin. The GFP-alpha-acti...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.94.17.9493

    authors: Dabiri GA,Turnacioglu KK,Sanger JM,Sanger JW

    更新日期:1997-08-19 00:00:00

  • Activity of synthetic tat peptides in human immunodeficiency virus type 1 long terminal repeat-promoted transcription in a cell-free system.

    abstract::The tat protein encoded by the human immunodeficiency virus type 1 is a potent trans-activator of gene expression from the viral long terminal repeat. The domains that are essential for trans-activation, a Pro-Xaa3-Pro triad, a cysteine-rich metal-binding sequence motif, and a cluster of basic residues, are present wi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.18.7030

    authors: Jeyapaul J,Reddy MR,Khan SA

    更新日期:1990-09-01 00:00:00

  • Satellite observations reveal extreme methane leakage from a natural gas well blowout.

    abstract::Methane emissions due to accidents in the oil and natural gas sector are very challenging to monitor, and hence are seldom considered in emission inventories and reporting. One of the main reasons is the lack of measurements during such events. Here we report the detection of large methane emissions from a gas well bl...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1908712116

    authors: Pandey S,Gautam R,Houweling S,Denier van der Gon H,Sadavarte P,Borsdorff T,Hasekamp O,Landgraf J,Tol P,van Kempen T,Hoogeveen R,van Hees R,Hamburg SP,Maasakkers JD,Aben I

    更新日期:2019-12-16 00:00:00

  • Hydrogen peroxide production, chemiluminescence, and the respiratory burst of fertilization: interrelated events in early sea urchin development.

    abstract::After fertilization of the sea urchin, Strongyl-ocentrotus purpuratus, a crosslinked fertilization membrane is formed; the crosslinks (dityrosine residues) are synthesized in a reaction catalyzed by an ovoperoxidase that is released from the cortical granules during fertilization. The substrate for ovoperoxidase activ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.75.7.3183

    authors: Foerder CA,Klebanoff SJ,Shapiro BM

    更新日期:1978-07-01 00:00:00

  • Loss of p53 synthesis in zebrafish tumors with ribosomal protein gene mutations.

    abstract::Zebrafish carrying heterozygous mutations for 17 different ribosomal protein (rp) genes are prone to developing malignant peripheral nerve sheath tumors (MPNSTs), a tumor type that is seldom seen in laboratory strains of zebrafish. Interestingly, the same rare tumor type arises in zebrafish that are homozygous for a l...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0805036105

    authors: MacInnes AW,Amsterdam A,Whittaker CA,Hopkins N,Lees JA

    更新日期:2008-07-29 00:00:00

  • Common polymorphism in the oxytocin receptor gene (OXTR) is associated with human social recognition skills.

    abstract::The neuropeptides oxytocin and vasopressin are evolutionarily conserved regulators of social perception and behavior. Evidence is building that they are critically involved in the development of social recognition skills within rodent species, primates, and humans. We investigated whether common polymorphisms in the g...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1302985111

    authors: Skuse DH,Lori A,Cubells JF,Lee I,Conneely KN,Puura K,Lehtimäki T,Binder EB,Young LJ

    更新日期:2014-02-04 00:00:00

  • Cell-free synthesis of the precursor polypeptide for avian myeloblastosis virus DNA polymerase.

    abstract::High molecular weight RNA (35S) isolated from avian myeloblastosis virus directs the cell-free synthesis of two prominent polypeptides of 180,000 and 76,000 molecular weight. The latter polypeptide has previously been identified as the precursor to the group-specific antigens of the virus ("gag" proteins) [Vogt, V. M....

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.74.11.4951

    authors: Paterson BM,Marciani DJ,Papas TS

    更新日期:1977-11-01 00:00:00

  • A mutation in segment IVS6 disrupts fast inactivation of sodium channels.

    abstract::Na(+)-channel inactivation is proposed to occur by binding of an intracellular inactivation gate to a hydrophobic inactivation gate receptor in the intracellular mouth of the pore. Amino acid residues in transmembrane segment S6 of domain IV (IVS6) that are critical for fast inactivation were identified by alanine-sca...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.25.12346

    authors: McPhee JC,Ragsdale DS,Scheuer T,Catterall WA

    更新日期:1994-12-06 00:00:00

  • Dependence receptor TrkC is a putative colon cancer tumor suppressor.

    abstract::The TrkC neurotrophin receptor belongs to the functional dependence receptor family, members of which share the ability to induce apoptosis in the absence of their ligands. Such a trait has been hypothesized to confer tumor-suppressor activity. Indeed, cells that express these receptors are thought to be dependent on ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1212333110

    authors: Genevois AL,Ichim G,Coissieux MM,Lambert MP,Lavial F,Goldschneider D,Jarrosson-Wuilleme L,Lepinasse F,Gouysse G,Herceg Z,Scoazec JY,Tauszig-Delamasure S,Mehlen P

    更新日期:2013-02-19 00:00:00

  • In vitro translation of hypoxanthine/guanine phosphoribosyltransferase mRNA: characterization of a mouse neuroblastoma cell line that has elevated levels of hypoxanthine/guanine phosphoribosyltransferase protein.

    abstract::Antibody specific for the native form of Chinese hamster hypoxanthine/guanine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) was used to detect the synthesis of HPRT protein in a rabbit reticulocyte lysate translation system primed with mRNA from Chinese hamster tissues and c...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.11.6977

    authors: Melton DW,Konecki DS,Ledbetter DH,Hejtmancik JF,Caskey CT

    更新日期:1981-11-01 00:00:00

  • Identification of an IL-27/osteopontin axis in dendritic cells and its modulation by IFN-gamma limits IL-17-mediated autoimmune inflammation.

    abstract::Dendritic cells (DCs) play a central role in determining the induction of T cell responses. IL-27 production by DCs favors induction of IL-10-producing regulatory T cells, whereas osteopontin (OPN) promotes pathogenic IL-17 T cell responses. The regulatory mechanisms in DCs that control these two cells types are not u...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1002099107

    authors: Murugaiyan G,Mittal A,Weiner HL

    更新日期:2010-06-22 00:00:00

  • Simultaneous mastering of two abstract concepts by the miniature brain of bees.

    abstract::Sorting objects and events into categories and concepts is a fundamental cognitive capacity that reduces the cost of learning every particular situation encountered in our daily lives. Relational concepts such as "same," "different," "better than," or "larger than"--among others--are essential in human cognition becau...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1202576109

    authors: Avarguès-Weber A,Dyer AG,Combe M,Giurfa M

    更新日期:2012-05-08 00:00:00

  • Structural basis for receptor subtype-specific regulation revealed by a chimeric beta 3/beta 2-adrenergic receptor.

    abstract::The physiological significance of multiple G-protein-coupled receptor subtypes, such as the beta-adrenergic receptors (beta ARs), remains obscure, since in many cases several subtypes activate the same effector and utilize the same physiological agonists. We inspected the deduced amino acid sequences of the beta AR su...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.8.3665

    authors: Liggett SB,Freedman NJ,Schwinn DA,Lefkowitz RJ

    更新日期:1993-04-15 00:00:00

  • Decentralization for cost-effective conservation.

    abstract::Since 1930, areas of state-managed forest in the central Himalayas of India have increasingly been devolved to management by local communities. This article studies the long-run effects of the devolution on the cost of forest management and on forest conservation. Village council-management costs an order of magnitude...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0810049106

    authors: Somanathan E,Prabhakar R,Mehta BS

    更新日期:2009-03-17 00:00:00