Abstract:
:The fate of two recently described human B lymphocyte-specific antigens (B1 and B2) was studied after B-cell activation in vivo and in vitro. Whereas both B1 and B2 were present on virtually all B cells from normal lymph nodes, B2 was absent from approximately 50% of B cells from hyperplastic lymph nodes. When B cells from spleen, tonsil, or peripheral blood were stimulated in vitro with pokeweed mitogen, activated cells were found to lose B2 (days 4-5) and subsequently B1 (days 6-7). Temporally, B2 loss was accompanied by loss of surface IgD, expression of T10, and the development of intracytoplasmic IgM; B1 loss was correlated with the acquisition of surface IgG and the appearance of intracytoplasmic IgG. Peripheral blood B cells, on which B2 is normally only weakly expressed (B1++++B2+) in contrast to B cells from secondary lymphoid organs (B1++++B2++), exhibited a transitory increase in B2 expression to the B1++++B2++ phenotype prior to B2 disappearance during activation. Taken together with other findings, this observation suggests that peripheral blood may contain a relatively immature subpopulation of B cells.
journal_name
Proc Natl Acad Sci U S Aauthors
Stashenko P,Nadler LM,Hardy R,Schlossman SFdoi
10.1073/pnas.78.6.3848subject
Has Abstractpub_date
1981-06-01 00:00:00pages
3848-52issue
6eissn
0027-8424issn
1091-6490journal_volume
78pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2015-04-21 00:00:00