当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > An approach to membrane protein structure without crystals.

An approach to membrane protein structure without crystals.

Abstract：

:The lactose permease of Escherichia coli catalyzes coupled translocation of galactosides and H(+) across the cell membrane. It is the best-characterized member of the Major Facilitator Superfamily, a related group of membrane proteins with 12 transmembrane domains that mediate transport of various substrates across cell membranes. Despite decades of effort and their functional importance in all kingdoms of life, no high-resolution structures have been solved for any member of this family. However, extensive biochemical, genetic, and biophysical studies on lactose permease have established its transmembrane topology, secondary structure, and numerous interhelical contacts. Here we demonstrate that this information is sufficient to calculate a structural model at the level of helix packing or better.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Sorgen PL,Hu Y,Guan L,Kaback HR,Girvin ME

doi

10.1073/pnas.182552199

keywords:

subject

Has Abstract

pub_date

2002-10-29 00:00:00

pages

14037-40

issue

22

eissn

0027-8424

issn

1091-6490

pii

182552199

journal_volume

99

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Fluorescent probe environment and the structural and charge changes in energy coupling of mitochondrial membranes.

    abstract：:The use of fluorescent probes to give continuous readouts of the structural states of mitochondrial membranes during energy coupling seems a logical extension of their use in the study of protein structural changes. A clear correlation of the probes' fluorescence characteristics with the acquisition of energy coupling...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.67.2.560

    authors： Chance B

    更新日期：1970-10-01 00:00:00

  • Radiocarbon-based approach capable of subannual precision resolves the origins of the site of Por-Bajin.

    abstract：:Inadequate resolution is the principal limitation of radiocarbon dating. However, recent work has shown that exact-year precision is attainable if use can be made of past increases in atmospheric radiocarbon concentration or so-called Miyake events. Here, this nascent method is applied to an archaeological site of pre...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1921301117

    authors： Kuitems M,Panin A,Scifo A,Arzhantseva I,Kononov Y,Doeve P,Neocleous A,Dee M

    更新日期：2020-06-23 00:00:00

  • Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells.

    abstract：:Vasopressin's action in renal cells to regulate water transport depends on protein phosphorylation. Here we used mass spectrometry-based quantitative phosphoproteomics to identify signaling pathways involved in the short-term V2-receptor-mediated response in cultured collecting duct cells (mpkCCD) from mouse. Using St...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0910646107

    authors： Rinschen MM,Yu MJ,Wang G,Boja ES,Hoffert JD,Pisitkun T,Knepper MA

    更新日期：2010-02-23 00:00:00

  • Targeting transcriptional regulation of SARS-CoV-2 entry factors ACE2 and TMPRSS2.

    abstract：:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for COVID-19, employs two key host proteins to gain entry and replicate within cells, angiotensin-converting enzyme 2 (ACE2) and the cell surface transmembrane protease serine 2 (TMPRSS2). TMPRSS2 was first characterized as an androgen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2021450118

    authors： Qiao Y,Wang XM,Mannan R,Pitchiaya S,Zhang Y,Wotring JW,Xiao L,Robinson DR,Wu YM,Tien JC,Cao X,Simko SA,Apel IJ,Bawa P,Kregel S,Narayanan SP,Raskind G,Ellison SJ,Parolia A,Zelenka-Wang S,McMurry L,Su F,Wang R,

    更新日期：2020-12-11 00:00:00

  • Corazonin receptor signaling in ecdysis initiation.

    abstract：:Corazonin is a highly conserved neuropeptide hormone of wide-spread occurrence in insects yet is associated with no universally recognized function. After discovery of the corazonin receptor in Drosophila, we identified its ortholog in the moth, Manduca sexta, as a prelude to physiological studies. The corazonin recep...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0305291101

    authors： Kim YJ,Spalovská-Valachová I,Cho KH,Zitnanova I,Park Y,Adams ME,Zitnan D

    更新日期：2004-04-27 00:00:00

  • Prestimulus influences on auditory perception from sensory representations and decision processes.

    abstract：:The qualities of perception depend not only on the sensory inputs but also on the brain state before stimulus presentation. Although the collective evidence from neuroimaging studies for a relation between prestimulus state and perception is strong, the interpretation in the context of sensory computations or decision...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1524087113

    authors： Kayser SJ,McNair SW,Kayser C

    更新日期：2016-04-26 00:00:00

  • A new form of cerebellar long-term potentiation is postsynaptic and depends on nitric oxide but not cAMP.

    abstract：:Long-term depression (LTD) at cerebellar parallel fiber (PF)-Purkinje cell synapses must be balanced by long-term potentiation (LTP) to prevent saturation and allow reversal of motor learning. The only previously analyzed form of cerebellar LTP is induced by 4-8 Hz PF stimulation and requires cAMP but not nitric oxide...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.122206399

    authors： Lev-Ram V,Wong ST,Storm DR,Tsien RY

    更新日期：2002-06-11 00:00:00

  • Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H.

    abstract：:A plasmid that consists of an 812-base-pair segment containing the replication origin of plasmid ColE1 and of a 1240-base-pair segment containing a beta-lactamase gene has been constructed. The plasmid DNA has three principal sites where transcription is initiated in vitro. One is located in the ColE1 segment 555 nucl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.5.2450

    authors： Itoh T,Tomizawa J

    更新日期：1980-05-01 00:00:00

  • Internalization and phagosome escape required for Francisella to induce human monocyte IL-1beta processing and release.

    abstract：:Macrophage responses to Francisella infection have been characterized previously by subdued proinflammatory responses; however, these studies have generally focused on macrophage cell lines or monocyte-derived macrophages. Therefore, we studied the ability of fresh human blood monocytes to engulf and respond to Franci...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0504271103

    authors： Gavrilin MA,Bouakl IJ,Knatz NL,Duncan MD,Hall MW,Gunn JS,Wewers MD

    更新日期：2006-01-03 00:00:00

  • The agroecological matrix as alternative to the land-sparing/agriculture intensification model.

    abstract：:Among the myriad complications involved in the current food crisis, the relationship between agriculture and the rest of nature is one of the most important yet remains only incompletely analyzed. Particularly in tropical areas, agriculture is frequently seen as the antithesis of the natural world, where the problem i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0905455107

    authors： Perfecto I,Vandermeer J

    更新日期：2010-03-30 00:00:00

  • Common polymorphism in the oxytocin receptor gene (OXTR) is associated with human social recognition skills.

    abstract：:The neuropeptides oxytocin and vasopressin are evolutionarily conserved regulators of social perception and behavior. Evidence is building that they are critically involved in the development of social recognition skills within rodent species, primates, and humans. We investigated whether common polymorphisms in the g...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1302985111

    authors： Skuse DH,Lori A,Cubells JF,Lee I,Conneely KN,Puura K,Lehtimäki T,Binder EB,Young LJ

    更新日期：2014-02-04 00:00:00

  • IL-1-induced NFkappa B and c-Jun N-terminal kinase (JNK) activation diverge at IL-1 receptor-associated kinase (IRAK).

    abstract：:Mutant I1A cells, lacking IL-1 receptor-associated kinase (IRAK) mRNA and protein, have been used to study the involvement of IRAK in NFkappaB and c-Jun N-terminal kinase (JNK) activation. A series of IRAK deletion constructs were expressed in I1A cells, which were then tested for their ability to respond to IL-1. Bot...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.071054198

    authors： Li X,Commane M,Jiang Z,Stark GR

    更新日期：2001-04-10 00:00:00

  • An operator at -280 base pairs that is required for repression of araBAD operon promoter: addition of DNA helical turns between the operator and promoter cyclically hinders repression.

    abstract：:A site has been found that is required for repression of the Escherichia coli araBAD operon. This site was detected by the in vivo properties of deletion mutants. In vitro protection studies with DNase I and dimethylsulfate showed that araC protein can specifically bind in this area to nucleotides lying at position -2...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.16.5017

    authors： Dunn TM,Hahn S,Ogden S,Schleif RF

    更新日期：1984-08-01 00:00:00

  • Expression of utrophin A mRNA correlates with the oxidative capacity of skeletal muscle fiber types and is regulated by calcineurin/NFAT signaling.

    abstract：:Utrophin levels have recently been shown to be more abundant in slow vs. fast muscles, but the nature of the molecular events underlying this difference remains to be fully elucidated. Here, we determined whether this difference is due to the expression of utrophin A or B, and examined whether transcriptional regulato...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0932671100

    authors： Chakkalakal JV,Stocksley MA,Harrison MA,Angus LM,Deschenes-Furry J,St-Pierre S,Megeney LA,Chin ER,Michel RN,Jasmin BJ

    更新日期：2003-06-24 00:00:00

  • Regulation of starvation- and virus-induced autophagy by the eIF2alpha kinase signaling pathway.

    abstract：:The eIF2alpha kinases are a family of evolutionarily conserved serine/threonine kinases that regulate stress-induced translational arrest. Here, we demonstrate that the yeast eIF2alpha kinase, GCN2, the target phosphorylation site of Gcn2p, Ser-51 of eIF2alpha, and the eIF2alpha-regulated transcriptional transactivato...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.012485299

    authors： Tallóczy Z,Jiang W,Virgin HW 4th,Leib DA,Scheuner D,Kaufman RJ,Eskelinen EL,Levine B

    更新日期：2002-01-08 00:00:00

  • A general model of invariant chain association with class II major histocompatibility complex proteins.

    abstract：:The binding of invariant chain to major histocompatibility complex (MHC) proteins is an important step in processing of MHC class II proteins and in antigen presentation. The question of how invariant chain can bind to all MHC class II proteins is central to understanding these processes. We have employed molecular mo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.18.8269

    authors： Lee C,McConnell HM

    更新日期：1995-08-29 00:00:00

  • A Ras activation pathway dependent on Syk phosphorylation of protein kinase C.

    abstract：:Protein kinase C (PKC) and Syk protein tyrosine kinase play critical roles in immune cell activation including that through the high-affinity IgE receptor, FcepsilonRI. Mechanisms by which PKC activation leads to the activation of Ras, a family of GTPases essential for immune cell activation, have been elusive. We pre...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1633695100

    authors： Kawakami Y,Kitaura J,Yao L,McHenry RW,Kawakami Y,Newton AC,Kang S,Kato RM,Leitges M,Rawlings DJ,Kawakami T

    更新日期：2003-08-05 00:00:00

  • Parkinson's disease-associated iPLA2-VIA/PLA2G6 regulates neuronal functions and α-synuclein stability through membrane remodeling.

    abstract：:Mutations in the iPLA2-VIA/PLA2G6 gene are responsible for PARK14-linked Parkinson's disease (PD) with α-synucleinopathy. However, it is unclear how iPLA2-VIA mutations lead to α-synuclein (α-Syn) aggregation and dopaminergic (DA) neurodegeneration. Here, we report that iPLA2-VIA-deficient Drosophila exhibits defects ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1902958116

    authors： Mori A,Hatano T,Inoshita T,Shiba-Fukushima K,Koinuma T,Meng H,Kubo SI,Spratt S,Cui C,Yamashita C,Miki Y,Yamamoto K,Hirabayashi T,Murakami M,Takahashi Y,Shindou H,Nonaka T,Hasegawa M,Okuzumi A,Imai Y,Hattori N

    更新日期：2019-10-08 00:00:00

  • Lipid monolayer image dipoles.

    abstract：:A simple model is described for calculating the electrostatic energy of lipid domains at the air-water interface, taking account of dipole-dipole repulsions between the lipid molecules themselves, as well as interactions between the molecular dipoles and image dipoles in the subphase. The model assumes that the molecu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.19.8823

    authors： McConnell HM,Bazaliy YB

    更新日期：1995-09-12 00:00:00

  • Interferon regulatory factor 4 differentially regulates the production of Th2 cytokines in naive vs. effector/memory CD4+ T cells.

    abstract：:Interferon regulatory factor (IRF) 4 is a member of the IRF family of transcription factors and plays critical roles in the development of CD4(+) T cells into Th2 and Th17 cells. Using the infection model of Nippostrongyrus brasiliensis, we have confirmed the critical roles of IRF-4 in Th2 development in vivo by using...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0803171105

    authors： Honma K,Kimura D,Tominaga N,Miyakoda M,Matsuyama T,Yui K

    更新日期：2008-10-14 00:00:00

  • Cell envelope and shape of Escherichia coli K12. Crosslinking with dimethyl imidoesters of the whole cell wall.

    abstract：:E. coli cells treated with the bifunctional crosslinking reagents dimethyl malonimidate, succinimidate, adipimidate, suberimidate, and sebacinimidate served for the isolation of rod-shaped "ghosts." These ghosts proved to be crosslinked over their entire surface; i.e., a macromolecule (resistant to boiling 1% Na dodec...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.5.2018

    authors： Haller I,Henning U

    更新日期：1974-05-01 00:00:00

  • The circadian clock controls the expression pattern of the circadian input photoreceptor, phytochrome B.

    abstract：:Developmental and physiological responses are regulated by light throughout the entire life cycle of higher plants. To sense changes in the light environment, plants have developed various photoreceptors, including the red/far-red light-absorbing phytochromes and blue light-absorbing cryptochromes. A wide variety of p...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.25.14652

    authors： Bognár LK,Hall A,Adám E,Thain SC,Nagy F,Millar AJ

    更新日期：1999-12-07 00:00:00

  • Structural basis underlying the dual gate properties of KcsA.

    abstract：:KcsA is a prokaryotic pH-dependent potassium (K) channel. Its activation, by a decrease in the intracellular pH, is coupled with its subsequent inactivation, but the underlying mechanisms remain elusive. Here, we have investigated the conformational changes and equilibrium of KcsA by using solution NMR spectroscopy. C...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0911270107

    authors： Imai S,Osawa M,Takeuchi K,Shimada I

    更新日期：2010-04-06 00:00:00

  • Discovery of sulfated metabolites in mycobacteria with a genetic and mass spectrometric approach.

    abstract：:The study of the metabolome presents numerous challenges, first among them being the cataloging of its constituents. A step in this direction will be the development of tools to identify metabolites that share common structural features. The importance of sulfated molecules in cell-cell communication motivated us to d...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.252514899

    authors： Mougous JD,Leavell MD,Senaratne RH,Leigh CD,Williams SJ,Riley LW,Leary JA,Bertozzi CR

    更新日期：2002-12-24 00:00:00

  • Trophic cascades in a complex terrestrial community.

    abstract：:To test for direct and indirect effects of a top predator on three lower trophic levels, we conducted two multiyear predator addition experiments in a tropical wet forest. Periodic additions of a top predator (predatory clerid beetle) to a wet forest understory shrub caused a reduction in the predatory beetle's prey (...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.9.5072

    authors： Dyer LA,Letourneau DK

    更新日期：1999-04-27 00:00:00

  • Ligand-directed retroviral targeting of human breast cancer cells.

    abstract：:We explored the feasibility of designing retroviral vectors that can target human breast cancer cells with characteristic receptors via ligand-receptor interaction. The ecotropic Moloney murine leukemia virus envelope was modified by insertion of sequences encoding human heregulin. Ecotropic virus, which normally does...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.21.9747

    authors： Han X,Kasahara N,Kan YW

    更新日期：1995-10-10 00:00:00

  • Crystal structure of dihydrofolate reductase from Plasmodium vivax: pyrimethamine displacement linked with mutation-induced resistance.

    abstract：:Pyrimethamine (Pyr) targets dihydrofolate reductase of Plasmodium vivax (PvDHFR) as well as other malarial parasites, but its use as antimalarial is hampered by the widespread high resistance. Comparison of the crystal structures of PvDHFR from wild-type and the Pyr-resistant (SP21, Ser-58 --> Arg + Ser-117 --> Asn) s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0501747102

    authors： Kongsaeree P,Khongsuk P,Leartsakulpanich U,Chitnumsub P,Tarnchompoo B,Walkinshaw MD,Yuthavong Y

    更新日期：2005-09-13 00:00:00

  • Tying synaptonemal complex initiation to the formation and programmed repair of DNA double-strand breaks.

    abstract：:During meiosis, homologous chromosomes recombine and become closely apposed along their lengths within the synaptonemal complex (SC). In part because Spo11 is required both to make the double-strand breaks (DSBs) that initiate recombination and to promote normal SC formation in many organisms, it is clear that these t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0400843101

    authors： Henderson KA,Keeney S

    更新日期：2004-03-30 00:00:00

  • PPARα-targeted mitochondrial bioenergetics mediate repair of intestinal barriers at the host-microbe intersection during SIV infection.

    abstract：:Chronic gut inflammatory diseases are associated with disruption of intestinal epithelial barriers and impaired mucosal immunity. HIV-1 (HIV) causes depletion of mucosal CD4+ T cells early in infection and disruption of gut epithelium, resulting in chronic inflammation and immunodeficiency. Although antiretroviral the...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1908977116

    authors： Crakes KR,Santos Rocha C,Grishina I,Hirao LA,Napoli E,Gaulke CA,Fenton A,Datta S,Arredondo J,Marco ML,Sankaran-Walters S,Cortopassi G,Giulivi C,Dandekar S

    更新日期：2019-12-03 00:00:00

  • Regulation of synthesis of cell-specific ribosomal proteins during differentiation of Dictyostelium discoideum.

    abstract：:Proteins of ribosomes from various stages of development in Dictyostelium discoideum were analyzed by two-dimensional polyacrylamide gel electrophoresis. Significant changes in protein composition were observed; the data demonstrate that cell differentiation in a eukaryotic system is accompanied by ribosome heterogene...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.5.3083

    authors： Ramagopal S,Ennis HL

    更新日期：1981-05-01 00:00:00