当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > The effect of overexpression of the protein tyrosine phosphatase PTPMEG on cell growth and on colony formation in soft agar in COS-7 cells.

The effect of overexpression of the protein tyrosine phosphatase PTPMEG on cell growth and on colony formation in soft agar in COS-7 cells.

Abstract：

:We established stable COS-7 cell lines overexpressing recombinant PTPMEG and an inactive mutant form in which the active site cysteine is mutated to serine (PTPMEGCS). We found that both endogenous and recombinant enzyme were primarily located in the membrane and cytoskeletal fractions of COS-7 cells. Endogenous PTPMEG accounts for only 1/3000th of the total tyrosine phosphatase activity in COS-7 cells and transfected cells expressed 2- to 7-fold higher levels of the enzyme. These levels of overexpression did not result in detectable changes in either total tyrosine phosphatase activity or the state of protein tyrosine phosphorylation as determined by immunoblotting of cell homogenates with anti-phosphotyrosine antibodies. Despite the low levels of activity for PTPMEG, we found that overexpressing cells grew slower and reached confluence at a lower density than vector transfected cells. Surprisingly, PTPMEGCS-transfected cells also reach confluence at a lower density than vector-transfected cells, although they grow to higher density than PTPMEG-transfected cells. Both constructs inhibited the ability of COS-7 cells to form colonies in soft agar, with the native PTPMEG having a greater effect (30-fold) than PTPMEGCS (10-fold). These results indicate that in COS-7 cells both PTPMEG and PTPMEGCS inhibit cell proliferation, reduce the saturation density, and block the ability of these cells to grow without adhering to a solid matrix.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Gu M,Meng K,Majerus PW

doi

10.1073/pnas.93.23.12980

subject

Has Abstract

pub_date

1996-11-12 00:00:00

pages

12980-5

issue

23

eissn

0027-8424

issn

1091-6490

journal_volume

93

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • The complete cDNA and deduced amino acid sequence of a type II mouse epidermal keratin of 60,000 Da: analysis of sequence differences between type I and type II keratins.

    abstract：:We present the complete nucleotide and deduced amino acid sequences of a mouse epidermal keratin subunit of 60,000 Da. The keratin possesses a central alpha-helical domain of four tracts (termed 1A, 1B, 2A, and 2B) that can form coiled-coils, interspersed by short linker sequences, and has non-alpha-helical terminal d...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.18.5709

    authors： Steinert PM,Parry DA,Racoosin EL,Idler WW,Steven AC,Trus BL,Roop DR

    更新日期：1984-09-01 00:00:00

  • Multiple movement modes by large herbivores at multiple spatiotemporal scales.

    abstract：:Recent theory suggests that animals should switch facultatively among canonical movement modes as a complex function of internal state, landscape characteristics, motion capacity, and navigational capacity. We tested the generality of this paradigm for free-ranging elk (Cervus elaphus) over 5 orders of magnitude in ti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0801737105

    authors： Fryxell JM,Hazell M,Börger L,Dalziel BD,Haydon DT,Morales JM,McIntosh T,Rosatte RC

    更新日期：2008-12-09 00:00:00

  • Kinesin-73 in the nervous system of Drosophila embryos.

    abstract：:Kinesin-73 cDNA was shown to encode a kinesin heavy chain protein that contains an N-terminal motor domain and a long central region that lacks extensive coiled-coils. The amino acid sequence of the motor domain of kinesin-73 protein is most closely related to the motor domains of Caenorhabditis elegans unc-104 and mo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.4.1086

    authors： Li HP,Liu ZM,Nirenberg M

    更新日期：1997-02-18 00:00:00

  • Regulation of bacterial sugar-H+ symport by phosphoenolpyruvate-dependent enzyme I/HPr-mediated phosphorylation.

    abstract：:The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a C-terminal hydrophilic domain that is homologous to IIA protein(s) domains of the phosphoenolpyruvate:sugar phosphotransferase system (PTS). C-terminal truncation mutants were constructed and expressed in Escherichia coli and their properties we...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.3.778

    authors： Poolman B,Knol J,Mollet B,Nieuwenhuis B,Sulter G

    更新日期：1995-01-31 00:00:00

  • Identification of a structural domain that distinguishes the actions of the type 1 and 2 isoforms of transforming growth factor beta on endothelial cells.

    abstract：:A chimeric transforming growth factor beta (TGF-beta) molecule has been synthesized to map the amino acids responsible for the substantially greater activity of TGF-beta 1 than TGF-beta 2 on growth and migration of endothelial cells. This chimera consists of a dimer of a monomeric unit composed of amino acids 1-39 of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.14.6290

    authors： Qian SW,Burmester JK,Merwin JR,Madri JA,Sporn MB,Roberts AB

    更新日期：1992-07-15 00:00:00

  • Defective CD95/APO-1/Fas signal complex formation in the human autoimmune lymphoproliferative syndrome, type Ia.

    abstract：:Heterozygous mutations in the CD95 (APO-1/Fas) receptor occur in most individuals with autoimmune lymphoproliferative syndrome (ALPS) and dominantly interfere with apoptosis by an unknown mechanism. We show that local or global alterations in the structure of the cytoplasmic death domain from nine independent ALPS CD9...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.8.4552

    authors： Martin DA,Zheng L,Siegel RM,Huang B,Fisher GH,Wang J,Jackson CE,Puck JM,Dale J,Straus SE,Peter ME,Krammer PH,Fesik S,Lenardo MJ

    更新日期：1999-04-13 00:00:00

  • Protein complex stoichiometry and expression dynamics of transcription factors modulate stem cell division.

    abstract：:Stem cells divide and differentiate to form all of the specialized cell types in a multicellular organism. In the Arabidopsis root, stem cells are maintained in an undifferentiated state by a less mitotically active population of cells called the quiescent center (QC). Determining how the QC regulates the surrounding ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2002166117

    authors： Clark NM,Fisher AP,Berckmans B,Van den Broeck L,Nelson EC,Nguyen TT,Bustillo-Avendaño E,Zebell SG,Moreno-Risueno MA,Simon R,Gallagher KL,Sozzani R

    更新日期：2020-06-30 00:00:00

  • The delta isoform of CaM kinase II is required for pathological cardiac hypertrophy and remodeling after pressure overload.

    abstract：:Acute and chronic injuries to the heart result in perturbation of intracellular calcium signaling, which leads to pathological cardiac hypertrophy and remodeling. Calcium/calmodulin-dependent protein kinase II (CaMKII) has been implicated in the transduction of calcium signals in the heart, but the specific isoforms o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0813013106

    authors： Backs J,Backs T,Neef S,Kreusser MM,Lehmann LH,Patrick DM,Grueter CE,Qi X,Richardson JA,Hill JA,Katus HA,Bassel-Duby R,Maier LS,Olson EN

    更新日期：2009-02-17 00:00:00

  • Randomly inserted and targeted Hox/reporter fusions transcriptionally silenced in Polycomb mutants.

    abstract：:Polycomb-group (Pc-G) proteins ensure late maintenance of transcriptional repression outside the expression domain of target genes in flies and vertebrates. They act in complexes, presumably by modulating chromatin structure. In Drosophila, they have been found to be associated with transcriptionally inactive loci but...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2237046100

    authors： d Graaff W,Tomotsune D,Oosterveen T,Takihara Y,Koseki H,Deschamps J

    更新日期：2003-11-11 00:00:00

  • Mice lacking pro-opiomelanocortin are sensitive to high-fat feeding but respond normally to the acute anorectic effects of peptide-YY(3-36).

    abstract：:Inactivating mutations of the pro-opiomelanocortin (POMC) gene in both mice and humans leads to hyperphagia and obesity. To further examine the mechanisms whereby POMC-deficiency leads to disordered energy homeostasis, we have generated mice lacking all POMC-derived peptides. Consistent with a previously reported mode...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0306931101

    authors： Challis BG,Coll AP,Yeo GS,Pinnock SB,Dickson SL,Thresher RR,Dixon J,Zahn D,Rochford JJ,White A,Oliver RL,Millington G,Aparicio SA,Colledge WH,Russ AP,Carlton MB,O'Rahilly S

    更新日期：2004-03-30 00:00:00

  • Evidence for different interleukin 1 receptors in murine B- and T-cell lines.

    abstract：:Previous studies have shown that binding of interleukin 1 (IL-1) to its receptor and intracellular processing of the IL-1/IL-1 receptor complex appear to be different in B- and T-lymphocyte cell lines. In this study we used a B-lymphoid cell line, 70Z/3, and T-lymphoid cell line, EL-4 6.1 C10, to explore further the d...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.20.8034

    authors： Bomsztyk K,Sims JE,Stanton TH,Slack J,McMahan CJ,Valentine MA,Dower SK

    更新日期：1989-10-01 00:00:00

  • Hydrodynamic narrowing of tubes extruded from cells.

    abstract：:We discuss the pulling force f required to extrude a lipid tube from a living cell as a function of the extrusion velocity L. The main feature is membrane friction on the cytoskeleton. As recently observed for neutrophils, the tether force exhibits a "shear thinning" response over a large range of pulling velocities, ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0602012103

    authors： Brochard-Wyart F,Borghi N,Cuvelier D,Nassoy P

    更新日期：2006-05-16 00:00:00

  • Assessment of individual radionuclide distributions from the Fukushima nuclear accident covering central-east Japan.

    abstract：:A tremendous amount of radioactivity was discharged because of the damage to cooling systems of nuclear reactors in the Fukushima No. 1 nuclear power plant in March 2011. Fukushima and its adjacent prefectures were contaminated with fission products from the accident. Here, we show a geographical distribution of radio...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1111724108

    authors： Kinoshita N,Sueki K,Sasa K,Kitagawa J,Ikarashi S,Nishimura T,Wong YS,Satou Y,Handa K,Takahashi T,Sato M,Yamagata T

    更新日期：2011-12-06 00:00:00

  • Regulatory T-cell expansion during chronic viral infection is dependent on endogenous retroviral superantigens.

    abstract：:Regulatory T cells (Treg) play critical roles in the modulation of immune responses to infectious agents. Further understanding of the factors that control Treg activation and expansion in response to pathogens is needed to manipulate Treg function in acute and chronic infections. Here we show that chronic, but not ac...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1100213108

    authors： Punkosdy GA,Blain M,Glass DD,Lozano MM,O'Mara L,Dudley JP,Ahmed R,Shevach EM

    更新日期：2011-03-01 00:00:00

  • Trans effect of the E1 region of adenoviruses on the expression of a prokaryotic gene in mammalian cells: resistance to 5' -CCGG- 3' methylation.

    abstract：:The plasmid construct pSVO-CAT has been used to test adenovirus promoter activities in the unmethylated or methylated state. We have now observed that the E2A late promoter of adenovirus type 2 (Ad2) DNA also activated the chloramphenicol acetyltransferase (CAT) gene upon transfection of the pAd2E2A-CAT construct into...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.6.1598

    authors： Langner KD,Weyer U,Doerfler W

    更新日期：1986-03-01 00:00:00

  • Corneal collagen fibril structure in three dimensions: Structural insights into fibril assembly, mechanical properties, and tissue organization.

    abstract：:The ability of the cornea to transmit light while being mechanically resilient is directly attributable to the formation of an extracellular matrix containing orthogonal sheets of collagen fibrils. The detailed structure of the fibrils and how this structure underpins the mechanical properties and organization of the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.111150598

    authors： Holmes DF,Gilpin CJ,Baldock C,Ziese U,Koster AJ,Kadler KE

    更新日期：2001-06-19 00:00:00

  • Foundational model of structural connectivity in the nervous system with a schema for wiring diagrams, connectome, and basic plan architecture.

    abstract：:The nervous system is a biological computer integrating the body's reflex and voluntary environmental interactions (behavior) with a relatively constant internal state (homeostasis)-- promoting survival of the individual and species. The wiring diagram of the nervous system's structural connectivity provides an obliga...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1015128107

    authors： Swanson LW,Bota M

    更新日期：2010-11-30 00:00:00

  • Heparanase-neutralizing antibodies attenuate lymphoma tumor growth and metastasis.

    abstract：:Heparanase is an endoglycosidase that cleaves heparan sulfate side chains of proteoglycans, resulting in disassembly of the extracellular matrix underlying endothelial and epithelial cells and associating with enhanced cell invasion and metastasis. Heparanase expression is induced in carcinomas and sarcomas, often ass...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1519453113

    authors： Weissmann M,Arvatz G,Horowitz N,Feld S,Naroditsky I,Zhang Y,Ng M,Hammond E,Nevo E,Vlodavsky I,Ilan N

    更新日期：2016-01-19 00:00:00

  • Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair.

    abstract：:Fanconi anemia (FA) is a recessive disorder characterized by congenital abnormalities, progressive bone-marrow failure, and cancer susceptibility. Cells from FA patients are hypersensitive to agents that produce DNA crosslinks and, after treatment with these agents, have pronounced chromosome breakage and other cytoge...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0407796102

    authors： Nakanishi K,Yang YG,Pierce AJ,Taniguchi T,Digweed M,D'Andrea AD,Wang ZQ,Jasin M

    更新日期：2005-01-25 00:00:00

  • Dynamic motility selection drives population segregation in a bacterial swarm.

    abstract：:Population expansion in space, or range expansion, is widespread in nature and in clinical settings. Space competition among heterogeneous subpopulations during range expansion is essential to population ecology, and it may involve the interplay of multiple factors, primarily growth and motility of individuals. Struct...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1917789117

    authors： Zuo W,Wu Y

    更新日期：2020-03-03 00:00:00

  • A simple generative model of collective online behavior.

    abstract：:Human activities increasingly take place in online environments, providing novel opportunities for relating individual behaviors to population-level outcomes. In this paper, we introduce a simple generative model for the collective behavior of millions of social networking site users who are deciding between different...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1313895111

    authors： Gleeson JP,Cellai D,Onnela JP,Porter MA,Reed-Tsochas F

    更新日期：2014-07-22 00:00:00

  • Tubular arrays of the actin-DNase I complex induced by gadolinium.

    abstract：:We describe the preparation and structural analysis of ordered tubular arrays of the actin-DNase I complex. These structures consist of helically stacked rings; each ring is 73 A thick, has a 240 A outer and a 120 A inner diameter, and has 7-fold rotational symmetry. The actin-DNase I complex forms tubes under conditi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.6.1669

    authors： Fowler WE,Buhle EL,Aebi U

    更新日期：1984-03-01 00:00:00

  • Antibiotics cause parthenogenetic Trichogramma (Hymenoptera/Trichogrammatidae) to revert to sex.

    abstract：:Completely parthenogenetic Trichogramma wasps can be rendered permanently bisexual by treatment with three different antibiotics or high temperatures. The evidence strongly suggests that maternally inherited microorganisms cause parthenogenesis in these wasps. Theories predict female-biased sex ratio in offspring unde...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.7.2424

    authors： Stouthamer R,Luck RF,Hamilton WD

    更新日期：1990-04-01 00:00:00

  • Repression by hematin of porphyrin biosynthesis in erythrocyte precursors in congenital erythropoietic porphyria.

    abstract：:Hematin administered intravenously in a patient with congenital erythropoietic porphyria evidently entered erythrocyte precursors in the bone marrow, producing the well-known negative feedback repression of porphyrin biosynthesis with marked decline of porphyrin concentrations in urine, circulating plasma, and erythro...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.2.278

    authors： Watson CJ,Bossenmaier I,Cardinal R,Petryka ZJ

    更新日期：1974-02-01 00:00:00

  • The entropic contributions in vitamin B12 enzymes still reflect the electrostatic paradigm.

    abstract：:The catalytic power of enzymes containing coenzyme B12 has been, in some respects, the "last bastion" for the strain hypothesis. Our previous study of this system established by a careful sampling that the major part of the catalytic effect is due to the electrostatic interaction between the ribose of the ado group an...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1503828112

    authors： Schopf P,Mills MJ,Warshel A

    更新日期：2015-04-07 00:00:00

  • Aphidicolin inhibits the synthesis and joining of short DNA fragments but not the union of 10-kilobase DNA replication intermediates.

    abstract：:DNA replication intermediates in human melanoma cells have been investigated by using the drug aphidicolin, which inhibits DNA polymerase alpha. In untreated cells, Okazaki fragments and 10-kilobase (kb) DNA intermediates are formed. In aphidicolin-treated cells, the replication fork is stopped and there is no formati...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.13.3996

    authors： Lönn U,Lönn S

    更新日期：1983-07-01 00:00:00

  • Surge of neurophysiological coherence and connectivity in the dying brain.

    abstract：:The brain is assumed to be hypoactive during cardiac arrest. However, the neurophysiological state of the brain immediately following cardiac arrest has not been systematically investigated. In this study, we performed continuous electroencephalography in rats undergoing experimental cardiac arrest and analyzed change...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1308285110

    authors： Borjigin J,Lee U,Liu T,Pal D,Huff S,Klarr D,Sloboda J,Hernandez J,Wang MM,Mashour GA

    更新日期：2013-08-27 00:00:00

  • Relative CO2/NH3 selectivities of AQP1, AQP4, AQP5, AmtB, and RhAG.

    abstract：:The water channel aquaporin 1 (AQP1) and certain Rh-family members are permeable to CO(2) and NH(3). Here, we use changes in surface pH (pH(S)) to assess relative CO(2) vs. NH(3) permeability of Xenopus oocytes expressing members of the AQP or Rh family. Exposed to CO(2) or NH(3), AQP1 oocytes exhibit a greater maxima...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0813231106

    authors： Musa-Aziz R,Chen LM,Pelletier MF,Boron WF

    更新日期：2009-03-31 00:00:00

  • Protective immunity and eosinophilia in IgE-deficient SJA/9 mice infected with Nippostrongylus brasiliensis and Trichinella spiralis.

    abstract：:To elucidate the roles of IgE antibody in host responses to helminth infection, selective IgE-deficient SJA/9 mice were infected with the nematode parasites Nippostrongylus brasiliensis, which elicits remarkable potentiated IgE production, and Trichinella spiralis, which induces strong anti-Trichinella IgE antibody pr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.12.4460

    authors： Watanabe N,Katakura K,Kobayashi A,Okumura K,Ovary Z

    更新日期：1988-06-01 00:00:00

  • Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2).

    abstract：:Like other guanine nucleotide-exchange proteins (GEPs) that activate ADP-ribosylation factor (ARF) GTPases, brefeldin A-inhibited GEP2, BIG2, contains an approximately 200-aa Sec7 domain that is responsible for this catalytic activity and its inhibition by brefeldin A. The Sec7 domain is located near the center of the...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0337678100

    authors： Li H,Adamik R,Pacheco-Rodriguez G,Moss J,Vaughan M

    更新日期：2003-02-18 00:00:00