Identification of a structural domain that distinguishes the actions of the type 1 and 2 isoforms of transforming growth factor beta on endothelial cells.

abstract：:The protein-protein interaction (PPI) network is crucial for cellular information processing and decision-making. With suitable inputs, PPI networks drive the cells to diverse functional outcomes such as cell proliferation or cell death. Here, we characterize the structural controllability of a large directed human PP...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vinayagam A,Gibson TE,Lee HJ,Yilmazel B,Roesel C,Hu Y,Kwon Y,Sharma A,Liu YY,Perrimon N,Barabási AL

更新日期：2016-05-03 00:00:00

abstract：:The increasing frequency of antibiotic resistance in hospital-acquired infections is a major public health concern that has both biological and economic causes. Here we develop conceptual mathematical models that couple the economic incentives and population biology of hospital infection control (HIC). We show that th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith DL,Levin SA,Laxminarayan R

更新日期：2005-02-22 00:00:00

abstract：:A tandemly repeated DNA sequence organized predominantly, if not entirely, in a specific manner on the human X chromosome has been cloned in pBR322 and characterized. The sequence was detected as a 2-kilobase band in ethidium bromide-stained agarose gels of BamHI-digested total human nuclear DNA. Although in situ hybr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang TP,Hansen SK,Oishi KK,Ryder OA,Hamkalo BA

更新日期：1982-11-01 00:00:00

abstract：:Analyses of genomic DNA and clones indicate that the pyruvate, orthophosphate dikinase (PPDK; ATP: pyruvate, orthophosphate phosphotransferase, EC 2.7.9.1) gene family of maize (Zea mays L. subsp. mays, line B73) contains two members. Restriction site and DNA sequence comparisons between PPDK genomic and leaf cDNA clo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glackin CA,Grula JW

更新日期：1990-04-01 00:00:00

abstract：:We present a model for transposition that allows a choice between cointegrate formation (replicon fusion) and direct transposition. We propose that initiation of the process occurs by invasion of the target DNA by a single-stranded end of the transposable element. This leads to nicking of one of the DNA strands of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Galas DJ,Chandler M

更新日期：1981-08-01 00:00:00

abstract：:Eradication of an invasive species can provide significant environmental, economic, and social benefits, but eradication programs often fail. Constant and careful monitoring improves the chance of success, but an invasion may seem to be in decline even when it is expanding in abundance or spatial extent. Determining w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keith JM,Spring D

更新日期：2013-08-13 00:00:00

abstract：:The current study identifies CCR8+ regulatory T cells (Treg cells) as drivers of immunosuppression. We show that in human peripheral blood cells, more than 30% of Treg up-regulate CCR8 following activation in the presence of CCL1. This interaction induces STAT3-dependent up-regulation of FOXp3, CD39, IL-10, and granzy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barsheshet Y,Wildbaum G,Levy E,Vitenshtein A,Akinseye C,Griggs J,Lira SA,Karin N

更新日期：2017-06-06 00:00:00

abstract：:Osteoclasts, bone-resorptive multinucleated cells derived from hematopoietic stem cells, are associated with many bone-related diseases, such as osteoporosis. Osteoclast-targeting small-molecule inhibitors are valuable tools for studying osteoclast biology and for developing antiresorptive agents. Here, we have discov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawatani M,Okumura H,Honda K,Kanoh N,Muroi M,Dohmae N,Takami M,Kitagawa M,Futamura Y,Imoto M,Osada H

更新日期：2008-08-19 00:00:00

abstract：:One goal of regenerative medicine is to repair damaged tissue. This requires not only generating new cells of the proper phenotype, but also selecting for those that properly integrate into sites of injury. In our laboratory we are using a cell-migration-based in vivo selection system to generate antibodies that induc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han KH,Arlian BM,Macauley MS,Paulson JC,Lerner RA

更新日期：2018-01-16 00:00:00

abstract：:Gastrulation is a fundamental process in early development that results in the formation of three primary germ layers. During avian gastrulation, presumptive mesodermal cells in the dorsal epiblast ingress through a furrow called the primitive streak (PS), and subsequently move away from the PS and form adult tissues....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zamir EA,Czirók A,Cui C,Little CD,Rongish BJ

更新日期：2006-12-26 00:00:00

abstract：:The progesterone receptor (PGR) is a ligand-activated transcription factor with key roles in the regulation of female fertility. Much has been learned of the actions of PGR signaling through the use of pharmacologic inhibitors and genetic manipulation, using mouse mutagenesis. Characterization of rats with a null muta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kubota K,Cui W,Dhakal P,Wolfe MW,Rumi MA,Vivian JL,Roby KF,Soares MJ

更新日期：2016-04-12 00:00:00

abstract：:The ability of beta cells to endure assaults may be relevant in the development of insulin-dependent diabetes mellitus. This study examines the susceptibility of human pancreatic islets to agents that are cytotoxic for rodent beta cells--i.e., sodium nitroprusside (NP, a nitric oxide donor), streptozotocin (SZ), or al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eizirik DL,Pipeleers DG,Ling Z,Welsh N,Hellerström C,Andersson A

更新日期：1994-09-27 00:00:00

abstract：:mRNA-dependent cell-free protein synthesis systems were prepared from a heme-deficient ole3 mutant of the yeast Saccharomyces cerevisiae grown either in the absence or in the presence of the heme precursor delta-aminolevulinate. When supplemented with total yeast mRNA, the two systems-from heme-deficient and from heme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hamilton B,Hofbauer R,Ruis H

更新日期：1982-12-01 00:00:00

abstract：:Ring-shaped hexameric helicases and translocases support essential DNA-, RNA-, and protein-dependent transactions in all cells and many viruses. How such systems coordinate ATPase activity between multiple subunits to power conformational changes that drive the engagement and movement of client substrates is a fundame...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomsen ND,Lawson MR,Witkowsky LB,Qu S,Berger JM

更新日期：2016-11-29 00:00:00

abstract：:We have grown crystals of a soluble recombinant form of human CD4, a transmembrane glycoprotein found predominantly on the surface of helper T cells. Crystals composed of the entire extracellular portion of CD4 exhibit extensive polymorphism. Of the five crystal types that have been grown, the best diffracts to Bragg ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kwong PD,Ryu SE,Hendrickson WA,Axel R,Sweet RM,Folena-Wasserman G,Hensley P,Sweet RW

更新日期：1990-08-01 00:00:00

abstract：:The complete amino acid sequences of the variable regions of two human IgM anti-gamma globulins are reported. The two proteins, Sie and Wol, share idiotypic determinants that are distinct from those shared by the two previously sequenced IgM anti-gamma globulins, Lay and Pom [Capra, J. D. & Kehoe, J. M. (1974) Proc. N...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Andrews DW,Capra JD

更新日期：1981-06-01 00:00:00

abstract：:Single molecule fluorescence polarization techniques have been used for three-dimensional (3D) orientation measurements to observe the dynamic properties of single molecules. However, only few techniques can simultaneously measure 3D orientation and position. Furthermore, these techniques often require complex equipme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohmachi M,Komori Y,Iwane AH,Fujii F,Jin T,Yanagida T

更新日期：2012-04-03 00:00:00

abstract：:The Ediacara biota include macroscopic, morphologically complex soft-bodied organisms that appear globally in the late Ediacaran Period (575-542 Ma). The physiology, feeding strategies, and functional morphology of the modular Ediacara organisms (rangeomorphs and erniettomorphs) remain debated but are critical for und...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laflamme M,Xiao S,Kowalewski M

更新日期：2009-08-25 00:00:00

abstract：:Strongylocentrotus intermedius (A. Agassiz, 1863) is an economically important sea urchin inhabiting the northwest Pacific region of Asia. The northern Primorye (Sea of Japan) populations of S. intermedius consist of two sympatric morphological forms, "usual" (U) and "gray" (G). The two forms are significantly differe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balakirev ES,Pavlyuchkov VA,Ayala FJ

更新日期：2008-10-21 00:00:00

abstract：:A rapid method for quantitating products of the oligodeoxynucleotide processing reaction in vitro has been developed to facilitate enzymatic studies of the retroviral integrases. Unlike earlier procedures, this assay does not depend on polyacrylamide gel electrphoresis but separates products by batch adsorption to PEI...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Müller B,Jones KS,Merkel GW,Skalka AM

更新日期：1993-12-15 00:00:00

abstract：:It is a well known fact that stirring keeps particles suspended in fluids. This is apparent, for instance, when shaking medicine flasks, when agitating tea deposits in a mug, or when heavy winds fill the air with dust particles. The commonplace nature of such observations makes it easy to accept that this feature will...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruiz J,Macías D,Peters F

更新日期：2004-12-21 00:00:00

abstract：:Sympathetic activation of β-adrenoreceptors (β-AR) represents a hallmark in the development of heart failure (HF). However, little is known about the underlying mechanisms of gene regulation. In human ventricular myocardium from patients with end-stage HF, we found high levels of phosphorylated histone 3 at serine-28 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saadatmand AR,Sramek V,Weber S,Finke D,Dewenter M,Sticht C,Gretz N,Wüstemann T,Hagenmueller M,Kuenzel SR,Meyer-Roxlau S,Kramer M,Sossalla S,Lehmann LH,Kämmerer S,Backs J,El-Armouche A

更新日期：2019-10-29 00:00:00

abstract：:Adult cardiomyocytes are irreversibly postmitotic but respond to a variety of stimuli by hypertrophic growth, which is associated with an increase in cell size and protein content, organization of sarcomeres, and activation of a fetal gene program. Recently, we described a novel cardiac helicase activated by MEF2 prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu ZP,Olson EN

更新日期：2002-02-19 00:00:00

abstract：:Individuals homozygous for the C-C chemokine receptor type 5 gene with 32-bp deletions (CCR5Δ32) are resistant to HIV-1 infection. In this study, we generated induced pluripotent stem cells (iPSCs) homozygous for the naturally occurring CCR5Δ32 mutation through genome editing of wild-type iPSCs using a combination of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ye L,Wang J,Beyer AI,Teque F,Cradick TJ,Qi Z,Chang JC,Bao G,Muench MO,Yu J,Levy JA,Kan YW

更新日期：2014-07-01 00:00:00

abstract：:Mutant, but not wild-type p53 binds with high affinity to a variety of MAR-DNA elements (MARs), suggesting that MAR-binding of mutant p53 relates to the dominant-oncogenic activities proposed for mutant p53. MARs recognized by mutant p53 share AT richness and contain variations of an AATATATTT "DNA-unwinding motif," w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Will K,Warnecke G,Wiesmüller L,Deppert W

更新日期：1998-11-10 00:00:00

abstract：:Two mutants of Balb/c 3T3 cells defective in adhesiveness to substratum have been isolated. After exposure to a mutagen a cell population was treated with prostaglandin E1 and methylisobutylxanthine to elevate the cells 3':5'-cyclic AMP levels and adhesion to the substratum; then, loosley adherent cells were harvested...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pouysségur JM,Pastan I

更新日期：1976-02-01 00:00:00

abstract：:Bipedalism is a defining trait of the hominin lineage, associated with a transition from a more arboreal to a more terrestrial environment. While there is debate about when modern human-like bipedalism first appeared in hominins, all known South African hominins show morphological adaptations to bipedalism, suggesting...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Georgiou L,Dunmore CJ,Bardo A,Buck LT,Hublin JJ,Pahr DH,Stratford D,Synek A,Kivell TL,Skinner MM

更新日期：2020-04-14 00:00:00

abstract：:When propagated on arl strains (a subclass of Escherichia coli hyper-rec mutants), lambda "Red-" duplication phages accumulated an enhanced potential for recombination. The physical properties of the recombinogenic phages thus obtained ("Arl-" phages) were similar to those of phages grown on arl+ bacteria. However, Ar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hays JB,Korba BE

更新日期：1979-12-01 00:00:00

abstract：:Experimental observations of unexpected shear rigidity in confined liquids, on very low frequency scales on the order of 0.01 to 0.1 Hz, call into question our basic understanding of the elasticity of liquids and have posed a challenge to theoretical models of the liquid state ever since. Here we combine the nonaffine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zaccone A,Trachenko K

更新日期：2020-08-18 00:00:00

abstract：:Mycobacterium marinum, a natural pathogen of fish and frogs and an occasional pathogen of humans, is capable of inducing actin tail formation within the cytoplasm of macrophages, leading to actin-based motility and intercellular spread. Actin tail formation by M. marinum is markedly reduced in macrophages deficient in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stamm LM,Pak MA,Morisaki JH,Snapper SB,Rottner K,Lommel S,Brown EJ

更新日期：2005-10-11 00:00:00