Molecular mechanisms of substrate-controlled ring dynamics and substepping in a nucleic acid-dependent hexameric motor.

abstract：:A rapid method for quantitating products of the oligodeoxynucleotide processing reaction in vitro has been developed to facilitate enzymatic studies of the retroviral integrases. Unlike earlier procedures, this assay does not depend on polyacrylamide gel electrphoresis but separates products by batch adsorption to PEI...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Müller B,Jones KS,Merkel GW,Skalka AM

更新日期：1993-12-15 00:00:00

abstract：:Transgenic mice have provided invaluable information about gene function and regulation. However, because of marked differences between rodents and primates, some areas of human biology such as early embryonic development, aging, and maternal-fetal interactions would be best studied in a nonhuman primate model. Here, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolfgang MJ,Eisele SG,Browne MA,Schotzko ML,Garthwaite MA,Durning M,Ramezani A,Hawley RG,Thomson JA,Golos TG

更新日期：2001-09-11 00:00:00

abstract：:The spleen is the lymphoid organ that induces immune responses toward blood-borne pathogens. Specialized macrophages in the splenic marginal zone are strategically positioned to phagocytose pathogens and cell debris, but are not known to play a role in the activation of T-cell responses. Here we demonstrate that splen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Backer R,Schwandt T,Greuter M,Oosting M,Jüngerkes F,Tüting T,Boon L,O'Toole T,Kraal G,Limmer A,den Haan JM

更新日期：2010-01-05 00:00:00

abstract：:Representational difference analysis was used to search for pathogens in multiple sclerosis brains. We detected a 341-nucleotide fragment that was 99.4% identical to the major DNA binding protein gene of human herpesvirus 6 (HHV-6). Examination of 86 brain specimens by PCR demonstrated that HHV-6 was present in > 70% ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Challoner PB,Smith KT,Parker JD,MacLeod DL,Coulter SN,Rose TM,Schultz ER,Bennett JL,Garber RL,Chang M

更新日期：1995-08-01 00:00:00

abstract：:An investigation by paramagnetic resonance spectroscopy of the photoreduction of ferredoxin, oxygenically by water and anoxygenically by a direct electron donor to photosystem I, led to the unexpected findings that different reductive mechanisms may be involved. Ferredoxin photoreduced by water was not reoxidized in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arnon DI,Tsujimoto HY,Tang GM

更新日期：1980-05-01 00:00:00

abstract：:The traG protein of the F sex factor is an inner membrane protein with a molecular weight of 116,000. Mutants in traG (or in traN) are able to trigger conjugal DNA replication even though they cannot efficiently form stable mating aggregates with F(-) cells. The traG protein (and the traN protein) probably acts in the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manning PA,Morelli G,Achtman M

更新日期：1981-12-01 00:00:00

abstract：:Kinesin-73 cDNA was shown to encode a kinesin heavy chain protein that contains an N-terminal motor domain and a long central region that lacks extensive coiled-coils. The amino acid sequence of the motor domain of kinesin-73 protein is most closely related to the motor domains of Caenorhabditis elegans unc-104 and mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li HP,Liu ZM,Nirenberg M

更新日期：1997-02-18 00:00:00

abstract：:Several polymorphisms of the transcription factor 4 (TCF4) have been shown to increase the risk for schizophrenia, particularly TCF4 rs9960767. This polymorphism is associated with impaired sensorimotor gating measured by prepulse inhibition--an established endophenotype of schizophrenia. We therefore investigated whe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Quednow BB,Brinkmeyer J,Mobascher A,Nothnagel M,Musso F,Gründer G,Savary N,Petrovsky N,Frommann I,Lennertz L,Spreckelmeyer KN,Wienker TF,Dahmen N,Thuerauf N,Clepce M,Kiefer F,Majic T,Mössner R,Maier W,Gallinat J,D

更新日期：2012-04-17 00:00:00

abstract：:Norharman enhanced the known mutagenicity of 2-acetylaminofluorene derivatives in the Salmonella test system. The mutagenicities of 2-acetylaminofluorene, 2-aminofluorene, and N-hydroxy-2-acetylaminofluorene were enhanced by norharman only when rat liver microsomal enzymes were added, whereas the mutagenicity of N-ace...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Umezawa K,Shirai A,Matsushima T,Sugimura T

更新日期：1978-02-01 00:00:00

abstract：:Five DNA fragments have been cloned from yeast, chicken, and mouse DNA that titrate lac repressor in an Escherichia coli lac+ I+Z+ wild-type strain when on a multi-copy plasmid. The five repressor-binding sequences have been identified by DNA sequence determinations and DNase cleavage-inhibition patterns. They share t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simons A,Tils D,von Wilcken-Bergmann B,Müller-Hill B

更新日期：1984-03-01 00:00:00

abstract：:Class I major histocompatibility complex (MHC) antigens are confronted with an apparently insurmountable dilemma. Each should show a binding preference to a common enough variety of nonapeptides, so that one relevant nonapeptide can be found in at least every other viral protein to provoke a cytotoxic T-cell response....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohno S

更新日期：1992-05-15 00:00:00

abstract：:Hydrogen/deuterium exchange followed by trapping of the labeled species in the aprotic solvent DMSO has been used to elucidate structure in both the burst-phase molten globule-folding intermediate of apomyoglobin and in an equilibrium intermediate that models the kinetic intermediate. Precise estimates can be made of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishimura C,Dyson HJ,Wright PE

更新日期：2005-03-29 00:00:00

abstract：:Ferredoxin:NADPH oxidoreductase (FNR) is a key enzyme of photosynthetic electron transport required for generation of reduction equivalents. Recently, two proteins were found to be involved in membrane-anchoring of FNR by specific interaction via a conserved Ser/Pro-rich motif: Tic62 and Trol. Our crystallographic stu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alte F,Stengel A,Benz JP,Petersen E,Soll J,Groll M,Bölter B

更新日期：2010-11-09 00:00:00

abstract：:On illuminating chloroplasts with "short-wavelength" monochromatic light that supports oxygen evolution, spectral evidence was obtained for a new photoreactive chloroplast component, provisionally designated C550, which shows a reversible decrease of absorbance with a maximum at 550 mmu. The light-induced absorbance c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knaff DB,Arnon DI

更新日期：1969-07-01 00:00:00

abstract：:Not having enough of what one needs has long been shown to have detrimental consequences for decision making. Recent work suggests that the experience of insufficient resources can create a "scarcity" mindset; increasing attention toward the scarce resource itself, but at the cost of attention for unrelated aspects. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huijsmans I,Ma I,Micheli L,Civai C,Stallen M,Sanfey AG

更新日期：2019-06-11 00:00:00

abstract：:The recent characterization of the prokaryotic Cys(2)His(2) zinc-finger domain, identified in Ros protein from Agrobacterium tumefaciens, has demonstrated that, although possessing a similar zinc coordination sphere, this domain is structurally very different from its eukaryotic counterpart. A search in the databases ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baglivo I,Russo L,Esposito S,Malgieri G,Renda M,Salluzzo A,Di Blasio B,Isernia C,Fattorusso R,Pedone PV

更新日期：2009-04-28 00:00:00

abstract：:Transcriptional repressors can be classified as short- or long-range, according to their range of activity. Functional analysis of identified short-range repressors has been carried out largely in transgenic Drosophila, but it is not known whether general properties of short-range repressors are evident in other types...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ryu JR,Olson LK,Arnosti DN

更新日期：2001-11-06 00:00:00

abstract：:Changes in tree growth rates can affect tree mortality and forest feedbacks to the global carbon cycle. As air temperature increases, evaporative demand also increases, increasing effective drought in forest ecosystems. Using a spatially comprehensive network of Douglas fir (Pseudotsuga menziesii) chronologies from 12...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Restaino CM,Peterson DL,Littell J

更新日期：2016-08-23 00:00:00

abstract：:Frogs (Anura) are one of the most diverse groups of vertebrates and comprise nearly 90% of living amphibian species. Their worldwide distribution and diverse biology make them well-suited for assessing fundamental questions in evolution, ecology, and conservation. However, despite their scientific importance, the evol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feng YJ,Blackburn DC,Liang D,Hillis DM,Wake DB,Cannatella DC,Zhang P

更新日期：2017-07-18 00:00:00

abstract：:The BAG6 complex was first identified as an upstream loading factor for tail-anchored membrane proteins entering the TRC40-dependent pathway for posttranslational delivery to the endoplasmic reticulum. Subsequently, BAG6 was shown to enhance the proteasomal degradation of mislocalized proteins by selectively promoting...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leznicki P,High S

更新日期：2012-11-20 00:00:00

abstract：:ErbB3 (HER3) is a member of the EGF receptor (EGFR) family of receptor tyrosine kinases, which, unlike the other three family members, contains a pseudo kinase in place of a tyrosine kinase domain. In cancer, ErbB3 activation is driven by a ligand-dependent mechanism through the formation of heterodimers with EGFR, Er...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee S,Greenlee EB,Amick JR,Ligon GF,Lillquist JS,Natoli EJ Jr,Hadari Y,Alvarado D,Schlessinger J

更新日期：2015-10-27 00:00:00

abstract：:The Arabidopsis HYL1 gene encodes a nuclear double-stranded RNA-binding protein. A knockout mutation of the hyl1 gene is recessive and pleiotropic, causing developmental abnormalities, increasing sensitivity to abscisic acid, and reducing sensitivity to auxin and cytokinin. We report that levels of several microRNAs (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han MH,Goud S,Song L,Fedoroff N

更新日期：2004-01-27 00:00:00

abstract：:Although potassium channels play a variety of roles in shaping the electrical properties of neurons, little is known about how these channels are constituted in neurons. To examine the assembly and physiological function of A-type K+ channels in mature differentiated neurons, we have developed a highly efficient gene ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaang BK,Pfaffinger PJ,Grant SG,Kandel ER,Furukawa Y

更新日期：1992-02-01 00:00:00

abstract：:The building evidence for the contribution of microbiota to human disease has spurred an effort to develop therapies that target the gut microbiota. This is particularly evident in inflammatory bowel diseases (IBDs), where clinical trials of fecal microbiota transplantation have shown some efficacy. To aid the develop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Britton GJ,Contijoch EJ,Spindler MP,Aggarwala V,Dogan B,Bongers G,San Mateo L,Baltus A,Das A,Gevers D,Borody TJ,Kaakoush NO,Kamm MA,Mitchell H,Paramsothy S,Clemente JC,Colombel JF,Simpson KW,Dubinsky MC,Grinspan A,

更新日期：2020-09-01 00:00:00

abstract：:The oxidative capacity of cat skeletal muscles (soleus, gracilis, and gracilis chronically stimulated for 28 days) was derived from the total mitochondrial content in the muscle, the surface area of mitochondrial inner membranes, and respiratory activities of isolated mitochondria. Mitochondrial content was estimated ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwerzmann K,Hoppeler H,Kayar SR,Weibel ER

更新日期：1989-03-01 00:00:00

abstract：:Thrombospondin, a 450-kDa multinodular glycoprotein with lectin-type activity, is found in human platelets, endothelial cells, fibroblasts, smooth muscle cells, monocytes, and granular pneumocytes. Thrombospondin interacts with heparin, fibrinogen, fibronectin, collagen, histidine-rich glycoprotein, and plasminogen. R...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Asch AS,Leung LL,Shapiro J,Nachman RL

更新日期：1986-05-01 00:00:00

abstract：:Executing gene circuits by cell-free transcription-translation into cell-sized compartments, such as liposomes, is one of the major bottom-up approaches to building minimal cells. The dynamic synthesis and proper self-assembly of macromolecular structures inside liposomes, the cytoskeleton in particular, stands as a c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garenne D,Libchaber A,Noireaux V

更新日期：2020-01-28 00:00:00

abstract：:The positive transcription elongation factor b (P-TEFb) is involved in physiological and pathological events including inflammation, cancer, AIDS, and cardiac hypertrophy. The balance between its active and inactive form is tightly controlled to ensure cellular integrity. We report that the transcriptional repressor C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cherrier T,Le Douce V,Eilebrecht S,Riclet R,Marban C,Dequiedt F,Goumon Y,Paillart JC,Mericskay M,Parlakian A,Bausero P,Abbas W,Herbein G,Kurdistani SK,Grana X,Van Driessche B,Schwartz C,Candolfi E,Benecke AG,Van Lin

更新日期：2013-07-30 00:00:00

abstract：:The bacterial RsgA, a circularly permutated GTPase, whose GTPase activity is dependent on the 30S ribosomal subunit, is a late-stage ribosome biogenesis factor involved in the 30S subunit maturation. The role of RsgA is to release another 30S biogenesis factor, RbfA, from the mature 30S subunit in a GTP-dependent mann...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo Q,Yuan Y,Xu Y,Feng B,Liu L,Chen K,Sun M,Yang Z,Lei J,Gao N

更新日期：2011-08-09 00:00:00

abstract：:Polymorphism in materials often leads to significantly different physical properties--the rutile and anatase polymorphs of TiO2 are a prime example. Polytypism is a special type of polymorphism, occurring in layered materials when the geometry of a repeating structural layer is maintained but the layer-stacking sequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luo H,Xie W,Tao J,Inoue H,Gyenis A,Krizan JW,Yazdani A,Zhu Y,Cava RJ

更新日期：2015-03-17 00:00:00