traG protein of the F sex factor of Escherichia coli K-12 and its role in conjugation.

abstract：:To relate transients of force by single kinesin molecules with the elementary steps of the ATPase cycle, we measured the time to force generation by kinesin after photorelease of ATP from caged ATP. Kinesin-coated beads were trapped by an infrared laser and brought onto microtubules fixed to a coverslip. Tension was a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Higuchi H,Muto E,Inoue Y,Yanagida T

更新日期：1997-04-29 00:00:00

abstract：:The kinetics for hydrogen exchange at individual base pairs in self-complementary deoxydodecanucleotide duplexes have been estimated from NMR saturation recovery measurements on the resolved imino protons as a function of temperature. The imino protons of dA . dT base pairs in the center of the fully alternating d(C-G...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patel DJ,Ikuta S,Kozlowski S,Itakura K

更新日期：1983-04-01 00:00:00

abstract：:The search for technosignatures from hypothetical galactic civilizations is going through a new phase of intense activity. For the first time, a significant fraction of the vast search space is expected to be sampled in the foreseeable future, potentially bringing informative data about the abundance of detectable ext...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grimaldi C,Marcy GW

更新日期：2018-10-16 00:00:00

abstract：:Visual processing depends critically on the receptive field (RF) properties of visual neurons. However, comprehensive characterization of RFs beyond the primary visual cortex (V1) remains a challenge. Here we report fine RF structures in secondary visual cortex (V2) of awake macaque monkeys, identified through a proje...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu L,She L,Chen M,Liu T,Lu HD,Dan Y,Poo MM

更新日期：2016-02-16 00:00:00

abstract：:The Kell blood group is a major antigenic system in human erythrocytes. Kell antigens reside on a 93-kDa membrane glycoprotein that is surface-exposed and associated with the underlying cytoskeleton. We isolated tryptic peptides and, based on the amino acid sequence of one of the peptides and by using the PCR, prepare...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee S,Zambas ED,Marsh WL,Redman CM

更新日期：1991-07-15 00:00:00

abstract：:Several different families of DNA viruses encode proteins that inactivate the cellular retinoblastoma tumor suppressor protein (pRb), which normally functions to bind E2F transcription factors and restrict expression of genes necessary for cellular processes including DNA replication. Human cytomegalovirus (HCMV) UL97...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kamil JP,Hume AJ,Jurak I,Münger K,Kalejta RF,Coen DM

更新日期：2009-09-29 00:00:00

abstract：:Morphine produces analgesia at opiate receptors expressed in nociceptive circuits. mu, delta, and kappa opiate receptor subtypes are expressed in circuits that can modulate nociception and receive inputs from endogenous opioid neuropeptide ligands. The roles played by each receptor subtype in nociceptive processing in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sora I,Takahashi N,Funada M,Ujike H,Revay RS,Donovan DM,Miner LL,Uhl GR

更新日期：1997-02-18 00:00:00

abstract：:The aldosterone-induced augmentation of Na+ transport in toad bladder was analyzed by comparing the hormonal actions on the transepithelial short-circuit current and on the amiloride-sensitive 22Na+ uptake in isolated membrane vesicles. Incubating bladders with 0.5 microM aldosterone for 3 hr evoked more than a 2-fold...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Asher C,Garty H

更新日期：1988-10-01 00:00:00

abstract：:The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the DNA lesions 1-methyladenine and 3-methylcytosine, which are generated in single-stranded stretches of DNA. AlkB is an alpha-ketoglutarate- and Fe(II)-dependent dioxygenase that oxidizes the relevant methyl groups...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duncan T,Trewick SC,Koivisto P,Bates PA,Lindahl T,Sedgwick B

更新日期：2002-12-24 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) like other herpesviruses, expresses sequentially immediate early (IE), early, and late genes during lytic infection. Evidence of ability to establish latent infection has not been available, but by analogy with other herpesviruses it could be expected that IE genes that regulate and transac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rotola A,Ravaioli T,Gonelli A,Dewhurst S,Cassai E,Di Luca D

更新日期：1998-11-10 00:00:00

abstract：:Autism spectrum disorders (ASDs) comprise a highly heritable, multifarious group of neurodevelopmental disorders, which are characterized by repetitive behaviors and impairments in social interactions. Point mutations have been identified in X-linked Neuroligin (NLGN) 3 and 4X genes in patients with ASDs and all of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bemben MA,Nguyen QA,Wang T,Li Y,Nicoll RA,Roche KW

更新日期：2015-02-24 00:00:00

abstract：:Approximately 15 per cent of the light chains from homogeneous immunoglobulins in patients with multiple myeloma contain an oligosaccharide group. Five human myeloma light chains that contained carbohydrate were studied. The sequence Asn-[unk]-Ser/Thr was at the site of carbohydrate attachment in all light chains. The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sox HC Jr,Hood L

更新日期：1970-07-01 00:00:00

abstract：:This article presents an agent-based computational model of civil violence. Two variants of the civil violence model are presented. In the first a central authority seeks to suppress decentralized rebellion. In the second a central authority seeks to suppress communal violence between two warring ethnic groups. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Epstein JM

更新日期：2002-05-14 00:00:00

abstract：:P elements have been cloned and sequenced from Drosophila nebulosa. Their sequences have diverged less than 6% from P elements of Drosophila melanogaster. However D. nebulosa P elements have nucleotide changes that close all four open reading frames found in the D. melanogaster P element. Microinjection experiments sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lansman RA,Shade RO,Grigliatti TA,Brock HW

更新日期：1987-09-01 00:00:00

abstract：:We have identified and isolated a presumptive leukemogenic provirus from myeloblasts of a chicken in which leukemia had been induced by avian myeloblastosis virus (AMV). Leukemic myeloblasts isolated from peripheral blood or from converted yolk sac cultures of various strains of chickens, regardless of the endogenous ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Souza LM,Komaromy MC,Baluda MA

更新日期：1980-05-01 00:00:00

abstract：:Seed germination is a complex trait of key ecological and agronomic significance. Few genetic factors regulating germination have been identified, and the means by which their concerted action controls this developmental process remains largely unknown. Using publicly available gene expression data from Arabidopsis th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bassel GW,Lan H,Glaab E,Gibbs DJ,Gerjets T,Krasnogor N,Bonner AJ,Holdsworth MJ,Provart NJ

更新日期：2011-06-07 00:00:00

abstract：:Pain is a major health concern even though numerous analgesic agents are available. Side effects and lack of wide-spectrum efficacy of current drugs justify efforts to better understand pain mechanisms. Stabilization of natural epoxy-fatty acids (EFAs) through inhibition of the soluble epoxide hydrolase (sEH) reduces ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Inceoglu B,Wagner K,Schebb NH,Morisseau C,Jinks SL,Ulu A,Hegedus C,Rose T,Brosnan R,Hammock BD

更新日期：2011-03-22 00:00:00

abstract：:We demonstrate here that yeast killer viruses, previously thought to be transmitted only by cytoplasmic mixing during division, mating, or other induced forms of cell fusion, are capable of extracellular transmission. Viral particles from standard K1 and K2 killer strains were used to inoculate sensitive cells of Sacc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： el-Sherbeini M,Bostian KA

更新日期：1987-06-01 00:00:00

abstract：:Neuronal hyperexcitability in both injured and adjacent uninjured neurons is associated with states of chronic injury and pain and is likely subject to neuroinflammatory processes. Chronic inflammatory responses are largely orchestrated by chemokines. One chemokine, monocyte chemoattractant protein-1 (MCP-1), in the p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： White FA,Sun J,Waters SM,Ma C,Ren D,Ripsch M,Steflik J,Cortright DN,Lamotte RH,Miller RJ

更新日期：2005-09-27 00:00:00

abstract：:Functional transcription elongation complexes can be formed by adding RNA polymerase in trans to a preformed nucleic acid construct. This construct consists of a double-stranded DNA fragment that contains a noncomplementary (permanent DNA bubble) region into which an RNA primer oligonucleotide has been hybridized. By ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daube SS,Hart CR,von Hippel PH

更新日期：1994-09-27 00:00:00

abstract：:Mutations are a hallmark of cancer. Normal cells minimize spontaneous mutations through the combined actions of polymerase base selectivity, 3' --> 5' exonucleolytic proofreading, mismatch correction, and DNA damage repair. To determine the consequences of defective proofreading in mammals, we created mice with a poin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldsby RE,Hays LE,Chen X,Olmsted EA,Slayton WB,Spangrude GJ,Preston BD

更新日期：2002-11-26 00:00:00

abstract：:Arf family GTP-binding proteins are best characterized as regulators of membrane traffic, but recent studies indicate an additional role in cytoskeletal organization. An Arf GTPase-activating protein of the centaurin beta family, ASAP1 (also known as centaurin beta4), binds Arf and two other known regulators of the ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Randazzo PA,Andrade J,Miura K,Brown MT,Long YQ,Stauffer S,Roller P,Cooper JA

更新日期：2000-04-11 00:00:00

abstract：:The NMR spectra at 220 MHz of lysinevasopressin and its precursors were measured in dimethyl sulfoxide, and the peaks were assigned to specific protons. Information about hydrogen bonding was obtained from the temperature coefficients of the chemical shifts. With these data, and with several chemical-shift positions a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Von Dreele PH,Brewster AI,Scheraga HA,Ferger MF,Du Vigneaud V

更新日期：1971-05-01 00:00:00

abstract：:Mutants of Arabidopsis that contain reduced levels of polyunsaturated fatty acids showed growth characteristics at 22 degrees C that were very similar to wild type. By contrast, at 12 degrees C, the mutants failed to undergo stem elongation during reproductive growth although they produced normal flowers and fertile s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miquel M,James D Jr,Dooner H,Browse J

更新日期：1993-07-01 00:00:00

abstract：:Phosphatidylinositol 3-kinases (PI 3-kinases) have been implicated in membrane trafficking in the secretory and endocytic pathways of yeast and mammalian cells, but the molecular mechanisms by which these lipid kinases operate are not known. Here we identify a protein of 170 kDa that is rapidly released from cell memb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patki V,Virbasius J,Lane WS,Toh BH,Shpetner HS,Corvera S

更新日期：1997-07-08 00:00:00

abstract：:Ethylenediaminetetraacetate and other divalent-cation-complexing agents greatly stimulate the cellular binding of DNA molecules to competent pneumococci, while the appearance of genetic transformants and nuclease-resistant DNA binding are completely inhibited. Based on this finding, we developed an experimental system...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seto H,Tomasz A

更新日期：1974-04-01 00:00:00

abstract：:Previously, we showed using a computational agent-based model that a group of animals moving together can make a collective decision on direction of motion, even if there is a conflict between the directional preferences of two small subgroups of "informed" individuals and the remaining "uninformed" individuals have n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leonard NE,Shen T,Nabet B,Scardovi L,Couzin ID,Levin SA

更新日期：2012-01-03 00:00:00

abstract：:Viruses are fundamental components of marine microbial communities that significantly influence oceanic productivity, biogeochemistry, and ecosystem processes. Despite their importance, the temporal activities and dynamics of viral assemblages in natural settings remain largely unexplored. Here we report the transcrip...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aylward FO,Boeuf D,Mende DR,Wood-Charlson EM,Vislova A,Eppley JM,Romano AE,DeLong EF

更新日期：2017-10-24 00:00:00

abstract：:Photosystem II (PSII) is an intrinsic membrane protein complex that functions as a light-driven water:plastoquinone oxidoreductase in oxygenic photosynthesis. Electron transport in PSII is associated with formation of reactive oxygen species (ROS) responsible for oxidative modifications of PSII proteins. In this study...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kumar A,Prasad A,Sedlářová M,Kale R,Frankel LK,Sallans L,Bricker TM,Pospíšil P

更新日期：2021-01-26 00:00:00

abstract：:We extend and apply theories of filled foam elasticity and failure to recently available data on foods. The predictions of elastic modulus and failure mode dependence on internal pressure and on wall integrity are borne out by photographic evidence of distortion and failure under compressive loading and under the loca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warner M,Thiel BL,Donald AM

更新日期：2000-02-15 00:00:00