Overexpression of an Aplysia shaker K+ channel gene modifies the electrical properties and synaptic efficacy of identified Aplysia neurons.

abstract：:Expansion of polyglutamine repeats in several unrelated proteins causes neurodegenerative diseases with distinct but related pathologies. To provide a model system for investigating common pathogenic features, we have examined the behavior of polyglutamine expansions expressed in Caenorhabditis elegans. The expression...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Satyal SH,Schmidt E,Kitagawa K,Sondheimer N,Lindquist S,Kramer JM,Morimoto RI

更新日期：2000-05-23 00:00:00

abstract：:Five DNA fragments have been cloned from yeast, chicken, and mouse DNA that titrate lac repressor in an Escherichia coli lac+ I+Z+ wild-type strain when on a multi-copy plasmid. The five repressor-binding sequences have been identified by DNA sequence determinations and DNase cleavage-inhibition patterns. They share t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simons A,Tils D,von Wilcken-Bergmann B,Müller-Hill B

更新日期：1984-03-01 00:00:00

abstract：:Unprecedented population declines and extinctions because of human activities, combined with a growing recognition that such losses affect the stability of ecosystems, underscore the need to better understand how populations persist naturally. We provide field experimental evidence that high biodiversity-in particular...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carr MH,Anderson TW,Hixon MA

更新日期：2002-08-20 00:00:00

abstract：:Kelp forests (Order Laminariales) form key biogenic habitats in coastal regions of temperate and Arctic seas worldwide, providing ecosystem services valued in the range of billions of dollars annually. Although local evidence suggests that kelp forests are increasingly threatened by a variety of stressors, no comprehe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krumhansl KA,Okamoto DK,Rassweiler A,Novak M,Bolton JJ,Cavanaugh KC,Connell SD,Johnson CR,Konar B,Ling SD,Micheli F,Norderhaug KM,Pérez-Matus A,Sousa-Pinto I,Reed DC,Salomon AK,Shears NT,Wernberg T,Anderson RJ,Barre

更新日期：2016-11-29 00:00:00

abstract：:Three fluorescein-labeled lectins have been shown to exhibit specificity for the surface of cells in different layers of the epidermis in the newborn rat. An isolectin from seeds of Bandeiraea simplicifolia with specificity for alpha-D-galactosyl end groups labeled the basal and lower spinous cells; a lectin from Ulex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brabec RK,Peters BP,Bernstein IA,Gray RH,Goldstein IJ

更新日期：1980-01-01 00:00:00

abstract：:The existence of immunoregulatory genes conferring dominant resistance to autoimmunity is well documented. In an effort to better understand the nature and mechanisms of action of these genes, we utilized the murine model of autoimmune orchitis as a prototype. When the orchitis-resistant strain DBA/2J is crossed with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meeker ND,Hickey WF,Korngold R,Hansen WK,Sudweeks JD,Wardell BB,Griffith JS,Teuscher C

更新日期：1995-06-06 00:00:00

abstract：:The genes encoding the variable (V) region of the B-cell antigen receptor (BCR) are assembled from V, D (diversity), and J (joining) elements through a RAG-mediated recombination process that relies on the recognition of recombination signal sequences (RSSs) flanking the individual elements. Secondary V(D)J rearrangem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun A,Novobrantseva TI,Coffre M,Hewitt SL,Jensen K,Skok JA,Rajewsky K,Koralov SB

更新日期：2015-02-03 00:00:00

abstract：:Sheep antibodies directed against an AMP-bovine serum albumin conjugate exhibit highly specific binding toward AMP. These antibodies bind to the AMP moiety of adenylylated glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] from Escherichia coli and to no other antigenic determinant on the prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hohman RJ,Rhee SG,Stadtman ER

更新日期：1980-12-01 00:00:00

abstract：:AIDS remains incurable due to the permanent integration of HIV-1 into the host genome, imparting risk of viral reactivation even after antiretroviral therapy. New strategies are needed to ablate the viral genome from latently infected cells, because current methods are too inefficient and prone to adverse off-target e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu W,Kaminski R,Yang F,Zhang Y,Cosentino L,Li F,Luo B,Alvarez-Carbonell D,Garcia-Mesa Y,Karn J,Mo X,Khalili K

更新日期：2014-08-05 00:00:00

abstract：:This paper introduces the use of magnetic field tomography (MFT), a noninvasive technique based on distributed source analysis of magnetoencephalography data, which makes possible the three-dimensional reconstruction of dynamic brain activity in humans. MFT has a temporal resolution better than 1 msec and a spatial ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ribary U,Ioannides AA,Singh KD,Hasson R,Bolton JP,Lado F,Mogilner A,Llinás R

更新日期：1991-12-15 00:00:00

abstract：:Mutations in Cu, Zn superoxide dismutase (SOD1) cause the neurodegenerative disease familial amyotrophic lateral sclerosis from an as-yet-unidentified toxic property(ies). Analysis in Saccharomyces cerevisiae of a broad range of human familial amyotrophic lateral sclerosis-linked SOD1 mutants (A4V, G37R, G41D, H46R, H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Corson LB,Strain JJ,Culotta VC,Cleveland DW

更新日期：1998-05-26 00:00:00

abstract：:Crystals of myosin subfragment 1 from avian skeletal muscle have been grown reproducibly. They diffract x-rays to at least 4.5-A resolution. The subfragment 1 crystallizes in space group P2(1)2(1)2(1) where a = 107 A, b = 117 A, and c = 278 A. The cell dimensions and intensity distribution on x-ray diffraction photogr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rayment I,Winkelmann DA

更新日期：1984-07-01 00:00:00

abstract：:The Eastern Arc Mountains of Tanzania and the Atlantic Forest of Brazil are two of the most fragmented biodiversity hotspots. Species-area relationships predict that their habitat fragments will experience a substantial loss of species. Most of these extinctions will occur over an extended time, and therefore, reconne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Newmark WD,Jenkins CN,Pimm SL,McNeally PB,Halley JM

更新日期：2017-09-05 00:00:00

abstract：:The ubiquitous aquaporin channels are able to conduct water across cell membranes, combining the seemingly antagonist functions of a very high selectivity with a remarkable permeability. Whereas molecular details are obvious keys to perform these tasks, the overall efficiency of transport in such nanopores is also str...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gravelle S,Joly L,Detcheverry F,Ybert C,Cottin-Bizonne C,Bocquet L

更新日期：2013-10-08 00:00:00

abstract：:We present a method for predicting protein folding class based on global protein chain description and a voting process. Selection of the best descriptors was achieved by a computer-simulated neural network trained on a data base consisting of 83 folding classes. Protein-chain descriptors include overall composition, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dubchak I,Muchnik I,Holbrook SR,Kim SH

更新日期：1995-09-12 00:00:00

abstract：:The severity of Helicobacter pylori-related disease is correlated with a pathogenicity island (the Cag region of about 26 genes) whose presence is associated with the up-regulation of an IL-8 cytokine inflammatory response in gastric epithelial cells. Statistical analysis of the Cag gene sequences calculated from the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu G,McDaniel TK,Falkow S,Karlin S

更新日期：1999-06-08 00:00:00

abstract：:The RNA virus, hepatitis E virus (HEV) is the most or second-most important cause of acute clinical hepatitis in adults throughout much of Asia, the Middle East, and Africa. In these regions it is an important cause of acute liver failure, especially in pregnant women who have a mortality rate of 20-30%. Until recentl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shukla P,Nguyen HT,Torian U,Engle RE,Faulk K,Dalton HR,Bendall RP,Keane FE,Purcell RH,Emerson SU

更新日期：2011-02-08 00:00:00

abstract：:Let X = Omega/Gamma be a compact quotient of an irreducible bounded symmetric domain Omega of rank >/=2 by a discrete group omega of automorphisms without fixed points. It is well known that the Kähler-Einstein metric g on X carries seminegative curvature (in the sense of Griffiths). I show that any Hermitian metric h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mok N

更新日期：1986-04-01 00:00:00

abstract：:The Ipl (Tssc3) gene lies in an extended imprinted region of distal mouse chromosome 7, which also contains the Igf2 gene. Expression of Ipl is highest in placenta and yolk sac, where its mRNA is derived almost entirely from the maternal allele. Ipl encodes a small cytoplasmic protein with a pleckstrin-homology (PH) d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frank D,Fortino W,Clark L,Musalo R,Wang W,Saxena A,Li CM,Reik W,Ludwig T,Tycko B

更新日期：2002-05-28 00:00:00

abstract：:Maltose-binding protein (MBP) is a two-domain protein that undergoes a ligand-mediated conformational rearrangement from an "open" to a "closed" structure on binding to maltooligosaccharides. To characterize the energy landscape associated with this transition, we have generated five variants of MBP with mutations loc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Millet O,Hudson RP,Kay LE

更新日期：2003-10-28 00:00:00

abstract：:Interaction of the alpha beta T cell receptor (TCR) with major histocompatibility (MHC) molecules occupied with any of a large collection of peptides derived from self proteins is a critical step in driving T cell "positive" selection in the thymus. Interaction with this same pool of self-peptide/MHC ligands deletes T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu CP,Crawford F,Marrack P,Kappler J

更新日期：1998-04-14 00:00:00

abstract：:The highly selective alpha 1-adrenergic receptor antagonist prazosin was used to identify binding sites having alpha-adrenergic specificity in rat hepatic plasma membranes. Solubilization of the membrane-bound receptors was achieved by incubation with the nonionic detergent digitonin, and binding activity was assayed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Graham RM,Hess HJ,Homcy CJ

更新日期：1982-04-01 00:00:00

abstract：:We have used wild-type and variants of the T-lymphoma cell line S49 to explore internalization and down-regulation of adenylate cyclase-linked beta-adrenergic receptors. Internalization was defined by the loss in "surface receptors" detected at 4 degrees C on intact cells by the antagonists [3H]CGP-12177 or [125I]iodo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mahan LC,Koachman AM,Insel PA

更新日期：1985-01-01 00:00:00

abstract：:In a metabolic study of human and mouse preimplantation embryos (preembryos), we measured glucose uptake and phosphorylation with nonradioactive 2-deoxyglucose (DG) as tracer. Initial experiments indicated an active hexose transport capacity, a property thought to be restricted in mammals to intestinal villi and kidne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chi MM,Manchester JK,Basuray R,Mahendra S,Strickler RC,McDougal DB Jr,Lowry OH

更新日期：1993-11-01 00:00:00

abstract：:The high-pressure behavior of amorphous selenium has been investigated with time-resolved diamond anvil cell synchrotron x-ray diffraction and computed microtomography techniques. A two-step dynamic crystallization process is observed in which the monoclinic phase crystallized from the amorphous selenium and gradually...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu H,Wang L,Xiao X,De Carlo F,Feng J,Mao HK,Hemley RJ

更新日期：2008-09-09 00:00:00

abstract：:Ethylenediaminetetraacetate and other divalent-cation-complexing agents greatly stimulate the cellular binding of DNA molecules to competent pneumococci, while the appearance of genetic transformants and nuclease-resistant DNA binding are completely inhibited. Based on this finding, we developed an experimental system...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seto H,Tomasz A

更新日期：1974-04-01 00:00:00

abstract：:O-GlcNAc glycosylation (or O-GlcNAcylation) is a dynamic, inducible posttranslational modification found on proteins associated with neurodegenerative diseases such as α-synuclein, amyloid precursor protein, and tau. Deletion of the O-GlcNAc transferase (ogt) gene responsible for the modification causes early postnata...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang AC,Jensen EH,Rexach JE,Vinters HV,Hsieh-Wilson LC

更新日期：2016-12-27 00:00:00

abstract：:It has been recognized since ancient times that malaria fever is highly periodic but the mechanism has been poorly understood. Malaria fever is related to the parasite growth cycle in erythrocytes. After a fixed period of replication, a mature parasite (schizont) causes the infected erythrocyte to rupture, releasing p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kwiatkowski D,Nowak M

更新日期：1991-06-15 00:00:00

abstract：:Mouse beta-globin in encoded in a discontinuous structural gene interrupted by a 550-base pair intervening sequence of DNA. Correspondingly, the mature beta-globin mRNA appears to be synthesized via a 15S precursor, the length of which roughly equals the total length of the coding and intervening sequences of the beta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tilghman SM,Curtis PJ,Tiemeier DC,Leder P,Weissmann C

更新日期：1978-03-01 00:00:00

abstract：:Perhydrohistrionicotoxin, at concentrations of 10(-12)-10(-7) M, depressed the current generated by iontophoretic application of acetylcholine to endplate regions of soleus and extensor digitorum longus muscles of rats. However, no changes in the amplitude or time course of spontaneous miniature endplate potentials or...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Albuquerque EX,Gage PW

更新日期：1978-03-01 00:00:00