Cross-species infections of cultured cells by hepatitis E virus and discovery of an infectious virus-host recombinant.

abstract：:Nuclease resistance and RNA affinity are key criteria in the search for optimal antisense nucleic acid modifications, but the origins of the various levels of resistance to nuclease degradation conferred by chemical modification of DNA and RNA are currently not understood. The 2'-O-aminopropyl (AP)-RNA modification di...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teplova M,Wallace ST,Tereshko V,Minasov G,Symons AM,Cook PD,Manoharan M,Egli M

更新日期：1999-12-07 00:00:00

abstract：:To investigate the molecular basis for the tissue-specific expression of the human CD2 gene, its chromatin configuration was assessed by determining DNase I hypersensitivity and the degree of methylation during T-cell lineage commitment and development. Tissue-specific DNase I-hypersensitive sites were found within th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wotton D,Flanagan BF,Owen MJ

更新日期：1989-06-01 00:00:00

abstract：:Fast-atom bombardment mass spectrometry was used to follow the time course of disulfide bond formation during in vitro refolding of recombinant human macrophage-colony-stimulating factor. The content of iodoacetamide-alkylated half-cystines in proteolytic peptides of trapped refolding intermediates collected at 0, 6, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glocker MO,Arbogast B,Milley R,Cowgill C,Deinzer ML

更新日期：1994-06-21 00:00:00

abstract：:The Escherichia coli galactokinase gene (galK) was inserted into a modified early region transcription unit of simian virus 40 (SV40) contained on a bacterial plasmid. Introduction of this pSVK vector into monkey, mouse, and hamster cell lines by transfection resulted in efficient expression of the bacterial galK gene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schümperli D,Howard BH,Rosenberg M

更新日期：1982-01-01 00:00:00

abstract：:Adaptive behavior requires animals to learn from experience. Ideally, learning should both promote choices that lead to rewards and reduce choices that lead to losses. Because the ventral striatum (VS) contains neurons that respond to aversive stimuli and aversive stimuli can drive dopamine release in the VS, it is po...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taswell CA,Costa VD,Murray EA,Averbeck BB

更新日期：2018-12-26 00:00:00

abstract：:L-2-hydroxyglutarate (L-2HG) has emerged as a putative oncometabolite that is capable of inhibiting enzymes involved in metabolism, chromatin modification, and cell differentiation. However, despite the ability of L-2HG to interfere with a broad range of cellular processes, this molecule is often characterized as a me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li H,Chawla G,Hurlburt AJ,Sterrett MC,Zaslaver O,Cox J,Karty JA,Rosebrock AP,Caudy AA,Tennessen JM

更新日期：2017-02-07 00:00:00

abstract：:Actin polymerization provides a powerful propulsion force for numerous types of cell motility. Although tremendous progress has been made in identifying the biochemical components necessary for actin-based motility, the precise biophysical mechanisms of force generation remain unclear. To probe the polymerization forc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Upadhyaya A,Chabot JR,Andreeva A,Samadani A,van Oudenaarden A

更新日期：2003-04-15 00:00:00

abstract：:Vaccinia virus encodes a multisubunit DNA-dependent RNA polymerase (EC 2.7.7.6) that is packaged in the infectious virus particle. This polymerase was found to contain a submolar polypeptide of approximately 85 kDa in addition to the core subunits, which consist of two larger and several smaller polypeptides. The poly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ahn BY,Moss B

更新日期：1992-04-15 00:00:00

abstract：:Membrane vesicles were extracted from etiolated and light-grown plants, a plant cell suspension culture, and an alga. Upon addition of ATP and Mg(2+), active Ca(2+) uptake into the vesicles against a concentration gradient was shown. The dependence of this uptake on ATP and Mg(2+) concentrations, pH, and temperature i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gross J,Marmé D

更新日期：1978-03-01 00:00:00

abstract：:The structural and functional organization of the cell nucleus has been investigated using three-dimensional reconstruction, immunoelectron microscopy, and high-resolution in situ autoradiography. Nuclear regions enriched in small nuclear ribonucleoprotein particles (snRNPs) form a reticular network within the nucleop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spector DL

更新日期：1990-01-01 00:00:00

abstract：:With efforts underway to develop a preventive human immunodeficiency virus type 1 (HIV-1) vaccine, it remains unclear which immune responses are sufficient to protect against infection and whether prior HIV-1 immunity can alter the subsequent course of HIV-1 infection. We investigated these issues in the context of a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McElrath MJ,Corey L,Greenberg PD,Matthews TJ,Montefiori DC,Rowen L,Hood L,Mullins JI

更新日期：1996-04-30 00:00:00

abstract：:Splice variants (SVs) of receptors for growth hormone-releasing hormone (GHRH) have been found in primary human prostate cancers and diverse human cancer cell lines. GHRH antagonists inhibit growth of various experimental human cancers, including pancreatic and colorectal, xenografted into nude mice or cultured in vit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Busto R,Schally AV,Varga JL,Garcia-Fernandez MO,Groot K,Armatis P,Szepeshazi K

更新日期：2002-09-03 00:00:00

abstract：:Genes encoded by mitochondrial DNA (mtDNA) exist in large numbers per cell but can be selected very rapidly as a result of unequal partitioning of mtDNA between germ cells during embryogenesis. However, empirical studies of this "bottlenecking" effect are rare because of the apparent scarcity of heteroplasmic individu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Leeuwen T,Vanholme B,Van Pottelberge S,Van Nieuwenhuyse P,Nauen R,Tirry L,Denholm I

更新日期：2008-04-22 00:00:00

abstract：:Excitation-evoked Ca(2+) influx is the fastest and most ubiquitous chemical trigger for cellular processes, including neurotransmitter release, muscle contraction, and gene expression. The voltage dependence and timing of Ca(2+) entry are thought to be functions of voltage-gated calcium (CaV) channels composed of a ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pantazis A,Savalli N,Sigg D,Neely A,Olcese R

更新日期：2014-12-23 00:00:00

abstract：:The breast and ovarian cancer suppressor BRCA2 controls the enzyme RAD51 during homologous DNA recombination (HDR) to preserve genome stability. BRCA2 binds to RAD51 through 8 conserved BRC repeat motifs dispersed in an 1127-residue region (BRCA2([BRC1-8])). Here, we show that BRCA2([BRC1-8]) exerts opposing effects o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shivji MK,Mukund SR,Rajendra E,Chen S,Short JM,Savill J,Klenerman D,Venkitaraman AR

更新日期：2009-08-11 00:00:00

abstract：:The functional capacities of CD8(+) T cells important for virus clearance are influenced by interactions with antigen presenting cells (APCs) and CD4(+) T cells during initial selection, subsequent expansion, and development of memory. Recently, investigators have shown that polyfunctional T cells correlate best with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ndhlovu ZM,Oelke M,Schneck JP,Griffin DE

更新日期：2010-02-23 00:00:00

abstract：:Neurobiological theories of awareness propose divergent accounts of the spatial extent of brain changes that support conscious perception. Whereas focal theories posit mostly local regional changes, global theories propose that awareness emerges from the propagation of neural signals across a broad extent of sensory a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Godwin D,Barry RL,Marois R

更新日期：2015-03-24 00:00:00

abstract：:Shell shape and shell thickness of the intertidal snail Littorina obtusata changed markedly between 1871 and 1984 in northern New England. Shells collected prior to 1900 were high-spired with thin walls, whereas shells collected in 1982-84 were low-spired with thick walls. An intertidal crab (Carcinus maenas) which pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seeley RH

更新日期：1986-09-01 00:00:00

abstract：:In many species that form pair bonds, males display to their mate after pair formation. These displays elevate the female's investment into the brood. This is a form of cooperation because without the display, female investment is reduced to levels that are suboptimal for both sexes. The presence of such displays is p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Servedio MR,Powers JM,Lande R,Price TD

更新日期：2019-11-12 00:00:00

abstract：:The poly(A) site of bovine prolactin (bPRL) mRNA was examined by phased priming of cDNA synthesis with oligodeoxynucleotides of the general sequence d(pT8-N-N'). The existence of multiple poly(A)-adjacent sequences in bPRL mRNA was indicated by the production of specific chain-termination fragments with at least three...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sasavage NL,Smith M,Gillam S,Woychik RP,Rottman FM

更新日期：1982-01-01 00:00:00

abstract：:The polyamines, putrescine, spermidine, and spermine, are essential polycations, intimately involved in the regulation of cellular proliferation. Although polyamines exert dynamic effects on the conformation of nucleic acids and macromolecular synthesis in vitro, their specific functions in vivo are poorly understood....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mandal S,Mandal A,Johansson HE,Orjalo AV,Park MH

更新日期：2013-02-05 00:00:00

abstract：:Overexpression of membrane proteins in Escherichia coli frequently leads to the formation of aggregates or inclusion bodies, which is undesirable for most studies. Ideally, one would like to optimize the expression conditions by monitoring simultaneously and rapidly both the amounts of properly folded and aggregated m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geertsma ER,Groeneveld M,Slotboom DJ,Poolman B

更新日期：2008-04-15 00:00:00

abstract：:The Pyrococcus furiosus (PF) ornithine carbamoyltransferase (OTCase; EC 2.1.3.3) is an extremely heat-stable enzyme that maintains about 50% of its activity after heat treatment for 60 min at 100 degrees C. To understand the molecular basis of thermostability of this enzyme, we have determined its three-dimensional st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Villeret V,Clantin B,Tricot C,Legrain C,Roovers M,Stalon V,Glansdorff N,Van Beeumen J

更新日期：1998-03-17 00:00:00

abstract：:Fast neurotransmission requires that docked synaptic vesicles be located near the presynaptic N-type or P/Q-type calcium channels. Specific protein-protein interactions between a synaptic protein interaction (synprint) site on N-type and P/Q-type channels and the presynaptic SNARE proteins syntaxin, SNAP-25, and synap...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim DK,Catterall WA

更新日期：1997-12-23 00:00:00

abstract：:Chronic pancreatitis (CP) is a progressive inflammatory disease of the pancreas, leading to its fibrotic destruction. There are currently no drugs that can stop or slow the progression of the disease. The etiology of the disease is multifactorial, whereas recurrent attacks of acute pancreatitis are thought to precede ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hao W,Komar HM,Hart PA,Conwell DL,Lesinski GB,Friedman A

更新日期：2017-05-09 00:00:00

abstract：:A 165-kilodalton (kDal) surface glycoprotein encoded by human chromosome 7 (SA-7) had been characterized by using antisera raised against human chromosome 7-containing somatic cell hybrids. We now present evidence that SA-7 is the human receptor for epidermal growth factor (EGF) and that these antisera recognize human...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carlin CR,Knowles BB

更新日期：1982-08-01 00:00:00

abstract：:Specific binding properties of the tritium-labeled delta opiate receptor agonist [3H][2-D-penicillamine, 5-D-penicillamine]enkephalin [( 3H][D-Pen2, D-Pen5]enkephalin) were characterized in the rat brain and in a mouse neuroblastoma-rat glioma hybrid cell line (NG 108-15). Saturation isotherms of [3H][D-Pen2, D-Pen5]e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akiyama K,Gee KW,Mosberg HI,Hruby VJ,Yamamura HI

更新日期：1985-04-01 00:00:00

abstract：:Neurofibrillary tangles are one of two lesions found in the brain of Alzheimer disease victims. With synthetic peptide fragments of human neurofilament NF-M17 (Glu-Glu-Lys-Gly-Lys-Ser-Pro- Val-Pro-Lys-Ser-Pro-Val-Glu-Glu-Lys-Gly, phosphorylated and unphosphorylated), CD studies were done to examine the effect of sodiu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fasman GD,Moore CD

更新日期：1994-11-08 00:00:00

abstract：:Liver ischemia and reperfusion injury (IRI) is a major challenge in liver surgery. Diet restriction reduces liver damage by increasing stress resistance; however, the underlying molecular mechanisms remain unclear. We investigated the preventive effect of 12-h fasting on mouse liver IRI. Partial warm hepatic IRI model...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miyauchi T,Uchida Y,Kadono K,Hirao H,Kawasoe J,Watanabe T,Ueda S,Okajima H,Terajima H,Uemoto S

更新日期：2019-07-02 00:00:00

abstract：:In a previous paper we described the induction by x-irradiation or radiation-induced leukemia virus-inoculation of two classes of lymphoid T-cell neoplasms: The first class, designated T-cell lymphoblastoma (TCLB), consists of growth-factor-dependent eudiploid cells that home to the spleen and give rise to splenic tum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haas M,Altman A,Rothenberg E,Bogart MH,Jones OW

更新日期：1984-03-01 00:00:00