Possible ideal lac operator: Escherichia coli lac operator-like sequences from eukaryotic genomes lack the central G X C pair.

abstract：:A central problem in understanding enzyme regulation is to define the conformational states that account for allosteric changes in catalytic activity. For Escherichia coli aspartate transcarbamoylase (ATCase; EC) the active, relaxed (R state) holoenzyme is generally assumed to be represented by the crystal structure o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Endrizzi JA,Beernink PT,Alber T,Schachman HK

更新日期：2000-05-09 00:00:00

abstract：:We report the nucleotide sequences of iaaM and iaaH, the genetic determinants for, respectively, tryptophan 2-monooxygenase and indoleacetamide hydrolase, the enzymes that catalyze the conversion of L-tryptophan to indoleacetic acid in the tumor-forming bacterium Pseudomonas syringae pv. savastanoi. The sequence analy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamada T,Palm CJ,Brooks B,Kosuge T

更新日期：1985-10-01 00:00:00

abstract：:Chemokine homologs are encoded by many large DNA viruses, suggesting that they contribute to control of host leukocyte transmigration and trafficking during viral infection. Murine cytomegalovirus carries a CC (beta) chemokine homolog gene giving rise to two related proteins, murine cytomegalovirus chemokine 1 and 2 (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saederup N,Lin YC,Dairaghi DJ,Schall TJ,Mocarski ES

更新日期：1999-09-14 00:00:00

abstract：:Ehrlich and Raven formally introduced the concept of stepwise coevolution using butterfly and angiosperm interactions in an attempt to account for the impressive biological diversity of these groups. However, many biologists currently envision butterflies evolving 50 to 30 million years (Myr) after the major angiosper...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wheat CW,Vogel H,Wittstock U,Braby MF,Underwood D,Mitchell-Olds T

更新日期：2007-12-18 00:00:00

abstract：:The skeletal muscle L-type Ca2+ channel is a complex of five subunits that is specifically localized in the triad. Its primary function is the rapid activation of Ca2+ release from cytoplasmic stores in a process called excitation-contraction coupling. To study the role of alpha1S-beta1a interactions in the incorporat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neuhuber B,Gerster U,Döring F,Glossmann H,Tanabe T,Flucher BE

更新日期：1998-04-28 00:00:00

abstract：:Differential scanning microcalorimetry provides a noninvasive method for studying heat evolution in living cells. We used this technique to measure the heat evolved by thioglycollate broth-elicited mouse macrophages, and the effects of NaF, KCN, cycloheximide, and cytochalasins B and D on this parameter. The total hea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loike JD,Silverstein SC,Sturtevant JM

更新日期：1981-10-01 00:00:00

abstract：:Gonadal steroids have traditionally been found to have short-term effects on the mating behavior of adult vertebrates, such that increased steroid hormone levels influence behavior over the course of a few days. Long-term effects also have been observed in which embryonic exposure to steroid hormone influences the sex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Crews D

更新日期：1991-05-01 00:00:00

abstract：:Phosphocholine (PC) is an abundant primary form of organic phosphate that is transported in plant xylem sap. Addition of PC to the perfusate of compressed Pi-starved sycamore cells monitored by 31P NMR spectroscopy resulted in an accumulation of PC and all the other phosphate esters in the cytoplasmic compartment. Add...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gout E,Bligny R,Roby C,Douce R

更新日期：1990-06-01 00:00:00

abstract：:Six alternative hypotheses for the phylogenetic origin of Bilateria are evaluated by using complete 18S rRNA gene sequences for 52 taxa. These data suggest that there is little support for three of these hypotheses. Bilateria is not likely to be the sister group of Radiata or Ctenophora, nor is it likely that Bilateri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collins AG

更新日期：1998-12-22 00:00:00

abstract：:Sessile marine organisms are prolific sources of biologically active natural products. However, these compounds are often found in highly variable amounts, with the abiotic and biotic factors governing their production remaining poorly understood. We present an approach that permits monitoring of in vivo natural produ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Esquenazi E,Jones AC,Byrum T,Dorrestein PC,Gerwick WH

更新日期：2011-03-29 00:00:00

abstract：:Our understanding of membranes and membrane lipid function has lagged far behind that of nucleic acids and proteins, largely because it is difficult to manipulate cellular membrane lipid composition. To help solve this problem, we show that methyl-α-cyclodextrin (MαCD)-catalyzed lipid exchange can be used to maximally...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li G,Kim J,Huang Z,St Clair JR,Brown DA,London E

更新日期：2016-12-06 00:00:00

abstract：:Key predictions of the Hubble law are inconsistent with direct observations on equitable complete samples of extragalactic sources in the optical, infrared, and x-ray wave bands-e.g., the predicted dispersion in apparent magnitude is persistently greatly in excess of its observed value, precluding an explanation via h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Segal IE

更新日期：1993-06-01 00:00:00

abstract：:Clostridium ljungdahlii is an anaerobic homoacetogen, able to ferment sugars, other organic compounds, or CO(2)/H(2) and synthesis gas (CO/H(2)). The latter feature makes it an interesting microbe for the biotech industry, as important bulk chemicals and proteins can be produced at the expense of CO(2), thus combining...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Köpke M,Held C,Hujer S,Liesegang H,Wiezer A,Wollherr A,Ehrenreich A,Liebl W,Gottschalk G,Dürre P

更新日期：2010-07-20 00:00:00

abstract：:Atherosclerosis is a complex disease resulting from the interaction of multiple genes. We have used the Ldlr knockout mouse model in an interspecific genetic cross to map atherosclerosis susceptibility loci. A total of 174 (MOLF/Ei x B6.129S7-Ldlr(tm1Her)) x C57BL/6J-Ldlr(tm1Her) backcross mice, homozygous for the Ldl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Welch CL,Bretschger S,Latib N,Bezouevski M,Guo Y,Pleskac N,Liang CP,Barlow C,Dansky H,Breslow JL,Tall AR

更新日期：2001-07-03 00:00:00

abstract：:In layered materials, a common mode of deformation involves buckling of the layers under tensile deformation in the direction perpendicular to the layers. The instability mechanism, which operates in elastic materials from geological to nanometer scales, involves the elastic contrast between different layers. In a reg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Makke A,Perez M,Lame O,Barrat JL

更新日期：2012-01-17 00:00:00

abstract：:Platelet factor 4 is shown to be a chemotactic protein for human polymorphonuclear leukocytes and monocytes at concentrations found in human serum and reached locally in injured tissue. The maximum chemotactic response to platelet factor 4 nearly equals that achieved with saturating concentrations of the chemotactic a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deuel TF,Senior RM,Chang D,Griffin GL,Heinrikson RL,Kaiser ET

更新日期：1981-07-01 00:00:00

abstract：:A membrane-associated folate binding protein (FBP) and a soluble FBP, which is released into the culture medium, have been purified from human KB cells using affinity chromatography. By NaDodSO4/PAGE, both proteins have an apparent Mr of approximately 42,000. However, in the presence of Triton X-100, the soluble FBP e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luhrs CA,Pitiranggon P,da Costa M,Rothenberg SP,Slomiany BL,Brink L,Tous GI,Stein S

更新日期：1987-09-01 00:00:00

abstract：:The electrical currents at the surface of the proximal portion of an isolated and transected lamprey spinal cord were measured with an extracellular vibrating probe. Soon after transection, currents of about 0.5 mA/cm2 enter the cut surface of the spinal cord. These currents fall to about a quarter of their initial va...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Borgens RB,Jaffe LF,Cohen MJ

更新日期：1980-02-01 00:00:00

abstract：:An important goal in biology is to predict from sequence data the high-resolution structures of proteins and the interactions that occur between them. In this paper, we describe a computational approach that can make these types of predictions for a series of coiled-coil dimers. Our method comprises a dual strategy th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keating AE,Malashkevich VN,Tidor B,Kim PS

更新日期：2001-12-18 00:00:00

abstract：:Maternal deletion of the NESP55 differentially methylated region (DMR) (delNESP55/ASdel3-4(m), delNAS(m)) from the GNAS locus in humans causes autosomal dominant pseudohypoparathyroidism type Ib (AD-PHP-Ib(delNASm)), a disorder of proximal tubular parathyroid hormone (PTH) resistance associated with loss of maternal G...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernández-Rebollo E,Maeda A,Reyes M,Turan S,Fröhlich LF,Plagge A,Kelsey G,Jüppner H,Bastepe M

更新日期：2012-04-24 00:00:00

abstract：:cDNAs representing the alpha subunit of polyomavirus enhancer binding protein 2 (PEBP2; also called PEA2) were isolated. The products of the cDNAs are highly homologous to that of Drosophila segmentation gene runt (run) for an N-proximal 128-amino acid region showing 66% identity. The run homology region encompasses t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ogawa E,Maruyama M,Kagoshima H,Inuzuka M,Lu J,Satake M,Shigesada K,Ito Y

更新日期：1993-07-15 00:00:00

abstract：:The protective effects of estrogen in the cardiovascular system result from both systemic effects and direct actions of the hormone on the vasculature. Two estrogen receptors have been identified, ERalpha and ERbeta. We demonstrated previously that estrogen inhibits the response to vascular injury in both wild-type an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karas RH,Hodgin JB,Kwoun M,Krege JH,Aronovitz M,Mackey W,Gustafsson JA,Korach KS,Smithies O,Mendelsohn ME

更新日期：1999-12-21 00:00:00

abstract：:Analysis of the activity and structure of lower vertebrate alcohol dehydrogenases reveals that relationships between the classical liver and yeast enzymes need not be continuous. Both the ethanol activity of class I-type alcohol dehydrogenase (alcohol:NAD+ oxidoreductase, EC 1.1.1.1) and the glutathione-dependent form...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Danielsson O,Jörnvall H

更新日期：1992-10-01 00:00:00

abstract：:It is shown that a very simple semiempirical equation represents quite accurately the thermodynamic properties of aqueous sodium chloride from 373 to 823 K. The equation comprises one Margules term and a Debye-Hückel term. Just the one Margules parameter is freely adjustable because the Debye-Hückel parameter is deter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pitzer KS,Li YG

更新日期：1983-12-01 00:00:00

abstract：:The binding of killer cell Ig-like Receptors (KIR) to their Class I MHC ligands was shown previously to be characterized by extremely rapid association and dissociation rate constants. During experiments to investigate the biochemistry of receptor-ligand binding in more detail, the kinetic parameters of the interactio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Valés-Gómez M,Erskine RA,Deacon MP,Strominger JL,Reyburn HT

更新日期：2001-02-13 00:00:00

abstract：:PUF proteins control both growth and differentiation in the C. elegans germ line. These conserved RNA-binding proteins inhibit expression of target mRNAs, either by repressing translation or promoting degradation. Previous studies showed that PUF-8, a PUF protein with striking similarity to human Pumilio, prevents ret...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bachorik JL,Kimble J

更新日期：2005-08-02 00:00:00

abstract：:Glycogen synthase kinase 3β (GSK3β) and cyclin-dependent kinase 5 (CDK5) are tau kinases and have been proposed to contribute to the pathogenesis of Alzheimer's disease. The 3D structures of these kinases are remarkably similar, which led us to hypothesize that both might be capable of binding cyclin proteins--the act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chow HM,Guo D,Zhou JC,Zhang GY,Li HF,Herrup K,Zhang J

更新日期：2014-11-11 00:00:00

abstract：:Feasting is one of humanity's most universal and unique social behaviors. Although evidence for feasting is common in the early agricultural societies of the Neolithic, evidence in pre-Neolithic contexts is more elusive. We found clear evidence for feasting on wild cattle and tortoises at Hilazon Tachtit cave, a Late ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Munro ND,Grosman L

更新日期：2010-08-31 00:00:00

abstract：:It has often been suggested that the frequently observed Watson-Crick base-pair compensatory substitutions in RNA helical structures occur mainly through a slightly deleterious G.U intermediate state. We have scored base substitutions in a set of 82 related Drosophila species for the D1 and D2 variable domains of the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rousset F,Pélandakis M,Solignac M

更新日期：1991-11-15 00:00:00

abstract：:Glucose is a fundamental metabolite, yet how cells sense and respond to changes in extracellular glucose concentration is not completely understood. We recently reported that the MondoA:Mlx dimeric transcription factor directly regulates glycolysis. In this article, we consider whether MondoA:Mlx complexes have a broa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stoltzman CA,Peterson CW,Breen KT,Muoio DM,Billin AN,Ayer DE

更新日期：2008-05-13 00:00:00