Abstract:
:Phosphocholine (PC) is an abundant primary form of organic phosphate that is transported in plant xylem sap. Addition of PC to the perfusate of compressed Pi-starved sycamore cells monitored by 31P NMR spectroscopy resulted in an accumulation of PC and all the other phosphate esters in the cytoplasmic compartment. Addition of hemicholinium-3, an inhibitor of choline uptake, to the perfusate inhibited PC accumulation but not inorganic phosphate (Pi). When the Pi-starved cells were perfused with a medium containing either Pi or PC, the resulting Pi distribution in the cell was the same. Addition of choline instead of PC to the perfusate of compressed cells resulted in an accumulation of PC in the cytoplasmic compartment from choline kinase activity. In addition, PC phosphatase activity has been discovered associated with the cell wall. These results indicate that PC was rapidly hydrolyzed outside the cell and that choline and Pi entered the cytosolic compartment where choline kinase re-forms PC.
journal_name
Proc Natl Acad Sci U S Aauthors
Gout E,Bligny R,Roby C,Douce Rdoi
10.1073/pnas.87.11.4280keywords:
subject
Has Abstractpub_date
1990-06-01 00:00:00pages
4280-3issue
11eissn
0027-8424issn
1091-6490journal_volume
87pub_type
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