Fluorescence microscopy for simultaneous observation of 3D orientation and movement and its application to quantum rod-tagged myosin V.

abstract：:We have studied ten phenotypically similar patients with complete androgen insensitivity. All of the patients tested had significantly elevated serum luteinizing hormone and plasma androgens within or above the normal adult male range. On the basis of specific dihydrotestosterone binding by skin fibroblasts, we identi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amrhein JA,Meyer WJ 3rd,Jones HW Jr,Migeon CJ

更新日期：1976-03-01 00:00:00

abstract：:The Ca2+/calmodulin (CaM)-dependent protein phosphatase calcineurin is rapidly phosphorylated (0.8 mol of 32PO4 per mol of 60-kDa subunit of calcineurin) by brain Ca2+/CaM-dependent protein kinase II (CaM-kinase II). This reaction requires the autophosphorylated, Ca2+-independent form of CaM-kinase II since Ca2+/CaM b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hashimoto Y,King MM,Soderling TR

更新日期：1988-09-01 00:00:00

abstract：:In cultures of spleen cells from normal mice, recombinant human granulocyte colony-stimulating factor (G-CSF) supported the formation of multipotential blast cell colonies. Serial replating of the blast cell colonies in the presence of G-CSF, however, failed to demonstrate any direct effect of G-CSF on murine multipot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ikebuchi K,Clark SC,Ihle JN,Souza LM,Ogawa M

更新日期：1988-05-01 00:00:00

abstract：:T cells are activated physiologically by triggering the T-cell receptor-CD3 complex. There is evidence that invariant accessory molecules on the T-cell membrane (CD8 and CD4) are involved in the major histocompatibility complex-restricted recognition process. Moreover, binding and crosslinking of these accessory molec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Samstag Y,Emmrich F,Staehelin T

更新日期：1988-12-01 00:00:00

abstract：:A nuclear protein, termed leucine-rich acidic nuclear protein (LANP), has been isolated from among rat cerebellar proteins whose expression was transiently increased during an early stage of postnatal development. The amino acid sequence, deduced from its cDNA, showed that LANP contains 247 amino acids consisting of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matsuoka K,Taoka M,Satozawa N,Nakayama H,Ichimura T,Takahashi N,Yamakuni T,Song SY,Isobe T

更新日期：1994-10-11 00:00:00

abstract：:Antibodies to the insulin receptor were prepared in BALB/c mice by immunization with IM-9 human lymphocytes, a cell type that has a large number of plasma membrane insulin receptors. The spleens of these mice were then removed, and their lymphocytes were fused to a mouse myeloma cell line, FO cells. After screening ov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roth RA,Cassell DJ,Wong KY,Maddux BA,Goldfine ID

更新日期：1982-12-01 00:00:00

abstract：:Nogo isoforms (Nogo-A and -B) have been implicated in regulating neural and cardiovascular functions, such as cell spreading and chemotaxis. Unlike the loop domain (Nogo-66) found in all Nogo isoforms that can interact with a neural-specific Nogo-66 receptor, the receptor for the amino terminus of Nogo-B that mediates...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miao RQ,Gao Y,Harrison KD,Prendergast J,Acevedo LM,Yu J,Hu F,Strittmatter SM,Sessa WC

更新日期：2006-07-18 00:00:00

abstract：:We use sequential large-scale crawl data to empirically investigate and validate the dynamics that underlie the evolution of the structure of the web. We find that the overall structure of the web is defined by an intricate interplay between experience or entitlement of the pages (as measured by the number of inbound ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kong JS,Sarshar N,Roychowdhury VP

更新日期：2008-09-16 00:00:00

abstract：:A major goal for developmental biologists is to define the behaviors and molecular contents of differentiating cells. We have devised a strategy for isolating cells from diverse embryonic regions and stages in the zebrafish, using computer-guided laser photoconversion of injected Kaede protein and flow cytometry. This...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown JL,Snir M,Noushmehr H,Kirby M,Hong SK,Elkahloun AG,Feldman B

更新日期：2008-08-26 00:00:00

abstract：:The suppressor of Hairy-wing [su(Hw)] protein exerts a polar effect on gene expression by repressing the function of transcriptional enhancers located distally from the promoter with respect to the location of su(Hw) binding sequences. The directionality of this effect suggests that the su(Hw) protein specifically int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Georgiev PG,Corces VG

更新日期：1995-05-23 00:00:00

abstract：:In animals and in man, diverse immunologic functions are mediated by specialized T-cell (thymus-derived lymphocyte) subsets that are distinguishable from one another on the basis of differences in cell surface determinants. Unfortunately, in humans, subset-specific antibodies have been difficult to generate. In this s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Engleman EG,Benike C,Osborne B,Goldsby R

更新日期：1980-03-01 00:00:00

abstract：:Although heparin and low-molecular-weight heparins (LMWH) have been widely used clinically as anticoagulants, their broader use has been limited by the lack of noninvasive delivery methods for this class of molecules. In this study, we demonstrate an efficient, rapid, and reproducible delivery system for heparin throu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qi Y,Zhao G,Liu D,Shriver Z,Sundaram M,Sengupta S,Venkataraman G,Langer R,Sasisekharan R

更新日期：2004-06-29 00:00:00

abstract：:The important role that cilia and flagella play in human disease creates an urgent need to identify genes involved in ciliary assembly and function. The strong and specific induction of flagellar-coding genes during flagellar regeneration in Chlamydomonas reinhardtii suggests that transcriptional profiling of such cel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stolc V,Samanta MP,Tongprasit W,Marshall WF

更新日期：2005-03-08 00:00:00

abstract：:Using a tissue culture system based on a nearly pure population of avian precartilage mesenchymal cells, we have found that ambient glucose levels as little as 50% lower, or 100% higher, than normally present in embryonic sera are deleterious to cartilage development, as measured by the accumulation of highly sulfated...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leonard CM,Bergman M,Frenz DA,Macreery LA,Newman SA

更新日期：1989-12-01 00:00:00

abstract：:Mutations at position 187 in secreted gelsolin enable aberrant proteolysis at the 172-173 and 243-244 amide bonds, affording the 71-residue amyloidogenic peptide deposited in Familial Amyloidosis of Finnish Type (FAF). Thermodynamic comparisons of two different domain 2 constructs were carried out to study possible ef...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ratnaswamy G,Huff ME,Su AI,Rion S,Kelly JW

更新日期：2001-02-27 00:00:00

abstract：:Small proteins can fold remarkably rapidly, even in μs. What limits their rate of folding? The Engrailed homeodomain is a particularly well-characterized example, which folds ultrafast via an intermediate, I, of solved structure. It is a puzzle that the helix2-turn-helix3 motif of the 3-helix bundle forms in approxima...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neuweiler H,Banachewicz W,Fersht AR

更新日期：2010-12-21 00:00:00

abstract：:In eukaryotes, transfer RNAs (tRNAs) are transcribed in the nucleus yet function in the cytoplasm; thus, tRNA movement within the cell was believed to be unidirectional--from the nucleus to the cytoplasm. It is now known that mature tRNAs also move in a retrograde direction from the cytoplasm to the nucleus via retrog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kramer EB,Hopper AK

更新日期：2013-12-24 00:00:00

abstract：:Dietary diversity often varies inversely with prey resource abundance. This pattern, although typically measured at the population level, is usually assumed to also characterize the behavior of individual animals within the population. However, the pattern might also be produced by changes in the degree of variation a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tinker MT,Bentall G,Estes JA

更新日期：2008-01-15 00:00:00

abstract：:Biodistribution studies of a water-soluble radioactive metallofullerene compound have been conducted using BALB/c mice. To this end, a sample containing Hox@C82 (x = 1, 2) was purified and derivatized to prepare the water-soluble metallofullerol, Hox@C82(OH)y. This metallofullerol was then neutron-activated (165Ho[n,g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cagle DW,Kennel SJ,Mirzadeh S,Alford JM,Wilson LJ

更新日期：1999-04-27 00:00:00

abstract：:Amyotrophic lateral sclerosis (ALS) is a progressive degenerative disease of motor neurons. The inherited form of the disease, familial ALS, represents 5-10% of the total cases, and the best documented of these are due to lesions in SOD1, the gene encoding copper-zinc superoxide dismutase (CuZnSOD). The mechanism by w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Valentine JS,Hart PJ

更新日期：2003-04-01 00:00:00

abstract：:Type 1A diabetes (T1D) is an autoimmune disorder the risk of which is increased by specific HLA DR/DQ alleles [e.g., DRB1*03-DQB1*0201 (DR3) or DRB1*04-DQB1*0302 (DR4)]. The genotype associated with the highest risk for T1D is the DR3/4-DQ8 (DQ8 is DQA1*0301, DQB1*0302) heterozygous genotype. We determined HLA-DR and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aly TA,Ide A,Jahromi MM,Barker JM,Fernando MS,Babu SR,Yu L,Miao D,Erlich HA,Fain PR,Barriga KJ,Norris JM,Rewers MJ,Eisenbarth GS

更新日期：2006-09-19 00:00:00

abstract：:Infection by certain human papillomavirus types is regarded as the major risk factor in the development of cervical cancer, one of the most common cancers of women worldwide. Analysis of the immunogenic and structural features of papillomavirus virions has been hampered by the inability to efficiently propagate the vi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirnbauer R,Booy F,Cheng N,Lowy DR,Schiller JT

更新日期：1992-12-15 00:00:00

abstract：:Host factor protein Cyclophilin A (CypA) regulates HIV-1 viral infectivity through direct interactions with the viral capsid, by an unknown mechanism. CypA can either promote or inhibit viral infection, depending on host cell type and HIV-1 capsid (CA) protein sequence. We have examined the role of conformational dyna...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu M,Hou G,Zhang H,Suiter CL,Ahn J,Byeon IJ,Perilla JR,Langmead CJ,Hung I,Gor'kov PL,Gan Z,Brey W,Aiken C,Zhang P,Schulten K,Gronenborn AM,Polenova T

更新日期：2015-11-24 00:00:00

abstract：:Goblet cell metaplasia and mucus hypersecretion are observed in many pulmonary diseases, including asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis. However, the regulation of goblet cell differentiation remains unclear. Here, we identify a regulator of this process in an N-ethyl-N-nitrosourea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim HT,Yin W,Nakamichi Y,Panza P,Grohmann B,Buettner C,Guenther S,Ruppert C,Kobayashi Y,Guenther A,Stainier DYR

更新日期：2019-12-17 00:00:00

abstract：:Bordetella pertussis, the causative agent of whooping cough, secretes several toxins implicated in this disease. One of these putative virulence factors is the adenylate cyclase (AC) toxin that elevates intracellular cAMP in eukaryotic cells to cytotoxic levels. This toxin is a bifunctional protein comprising both AC ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gross MK,Au DC,Smith AL,Storm DR

更新日期：1992-06-01 00:00:00

abstract：:Genetically engineered mice with targeted disruption of the neuronal nitric oxide synthase (nNOS) gene established the inhibitory role of nitric oxide (NO) in male impulsive aggressive behavior. This was later confirmed by using selective nNOS inhibitors in male wild-type mice. The molecular mechanisms accounting for ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chiavegatto S,Dawson VL,Mamounas LA,Koliatsos VE,Dawson TM,Nelson RJ

更新日期：2001-01-30 00:00:00

abstract：:cDNA clones encoding a glucose transporter-like protein have been isolated from adult human liver and kidney cDNA libraries by cross-hybridization with the human HepG2/erythrocyte glucose transporter cDNA. Analysis of the sequence of this 524-amino acid glucose transporter-like protein indicates that it has 55.5% iden...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fukumoto H,Seino S,Imura H,Seino Y,Eddy RL,Fukushima Y,Byers MG,Shows TB,Bell GI

更新日期：1988-08-01 00:00:00

abstract：:The energy landscape theory provides a general framework for describing protein folding reactions. Because a large number of studies, however, have focused on two-state proteins with single well-defined folding pathways and without detectable intermediates, the extent to which free energy landscapes are shaped up by t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Calosci N,Chi CN,Richter B,Camilloni C,Engström A,Eklund L,Travaglini-Allocatelli C,Gianni S,Vendruscolo M,Jemth P

更新日期：2008-12-09 00:00:00

abstract：:As the most prototypical G protein-coupled receptor, beta-adrenergic receptor (betaAR) regulates the pace and strength of heart beating by enhancing and synchronizing L-type channel (LCC) Ca(2+) influx, which in turn elicits greater sarcoplasmic reticulum (SR) Ca(2+) release flux via ryanodine receptors (RyRs). Howeve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou P,Zhao YT,Guo YB,Xu SM,Bai SH,Lakatta EG,Cheng H,Hao XM,Wang SQ

更新日期：2009-10-20 00:00:00

abstract：:Incommensurate charge order in hole-doped oxides is intertwined with exotic phenomena such as colossal magnetoresistance, high-temperature superconductivity, and electronic nematicity. Here, we map, at atomic resolution, the nature of incommensurate charge-lattice order in a manganite using scanning transmission elect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： El Baggari I,Savitzky BH,Admasu AS,Kim J,Cheong SW,Hovden R,Kourkoutis LF

更新日期：2018-02-13 00:00:00