Abstract:
:The important role that cilia and flagella play in human disease creates an urgent need to identify genes involved in ciliary assembly and function. The strong and specific induction of flagellar-coding genes during flagellar regeneration in Chlamydomonas reinhardtii suggests that transcriptional profiling of such cells would reveal new flagella-related genes. We have conducted a genome-wide analysis of RNA transcript levels during flagellar regeneration in Chlamydomonas by using maskless photolithography method-produced DNA oligonucleotide microarrays with unique probe sequences for all exons of the 19,803 predicted genes. This analysis represents previously uncharacterized whole-genome transcriptional activity profiling study in this important model organism. Analysis of strongly induced genes reveals a large set of known flagellar components and also identifies a number of important disease-related proteins as being involved with cilia and flagella, including the zebrafish polycystic kidney genes Qilin, Reptin, and Pontin, as well as the testis-expressed tubby-like protein TULP2.
journal_name
Proc Natl Acad Sci U S Aauthors
Stolc V,Samanta MP,Tongprasit W,Marshall WFdoi
10.1073/pnas.0408358102keywords:
subject
Has Abstractpub_date
2005-03-08 00:00:00pages
3703-7issue
10eissn
0027-8424issn
1091-6490pii
0408358102journal_volume
102pub_type
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